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The map label for this gene is cysK [H]

Identifier: 119774645

GI number: 119774645

Start: 1831345

End: 1832313

Strand: Reverse

Name: cysK [H]

Synonym: Sama_1508

Alternate gene names: 119774645

Gene position: 1832313-1831345 (Counterclockwise)

Preceding gene: 119774649

Following gene: 119774643

Centisome position: 42.55

GC content: 55.21

Gene sequence:

>969_bases
ATGAGCAAAATCTTTGAAGACAATTCTTACACGATTGGCAATACCCCCCTGGTACGATTGAACCGTGTCAGCAACGGCAA
GGTGCTGGCCAAGGTTGAAGCCCGTAACCCCAGCTTCTCTGTAAAGTGCCGTATCGGCGCCAACATGATTTGGGATGCCG
AAAAGCGTGGCGTGCTGACCAAAGACAAGGAACTCATCGAACCTACGTCCGGTAACACTGGTATTGCGCTGGCCTATGTG
GCCGCGGCCCGTGGTTACAAACTGACCCTGACCATGCCCAACACCATGAGCCTGGAGCGCCGTAAGCTGCTCAAGGCACT
GGGTGCCAACCTGGTGCTGACCGAAGGCGCCAAGGGCATGAAAGGCGCTATCGAAAAAGCCGAAGAAATCCGTCAGTCTG
CACCGGAAAAATACGTTCTGCTGCAGCAGTTTGACAACCCTGCCAACCCGGAAATCCACGAAAAAACCACAGGCCCAGAG
ATCTGGAATGATACCGACGGTGCCGTTGACGTGGTGGTGGCCGGTGTGGGTACCGGTGGTACCATCACAGGTGTGAGCCG
CTATATCAAGAAGGCACAGGGCAAGGCCATAGTGTCTGTGGCCGTTGAGCCTGTTGACTCACCGGTTATCAGCCAAACAC
TTGCTGGTCAGCCTGTTCAGCCAGGTCCGCACAAAATTCAGGGCATTGGCGCCGGTTTTATTCCGGGCAACCTTGACCTT
GAACTGATTGACCGTGTTGAAACCGTCAGCAACGAAGACGCCATCGAGATGGCCCACCGCCTGATGAAGGAAGAAGGCAT
TCTGGTGGGCATTTCCTCCGGTGCAGCCGTGGTTGCCGCCAACCGCATCGCAGCCCTGCCCGAGTTTGCTGATAAAAACA
TCGTGGTCATACTGCCATCCGCCGCTGAACGTTACCTGTCTTCAGTACTGTTCCAGGGTCAGTTTGGCGACCAGGAAAAC
GTGCAGTAA

Upstream 100 bases:

>100_bases
TTTAATAACTAAAAGAAATAACGAAGAACAAAGAATCACTTCTCAATGTCGTAACAGCGTTTATGCTTAAATGCGATAAA
GTCACAGGAGCTGCATTCCC

Downstream 100 bases:

>100_bases
GCCGTCAACGTAATATACGTGCGATGGGTTGATTAATGACAACCCACACAAAAAAGCCCGGTCCCAATGATTTGGGGGCG
GGCTTTTTACATGAAATCGT

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSKIFEDNSYTIGNTPLVRLNRVSNGKVLAKVEARNPSFSVKCRIGANMIWDAEKRGVLTKDKELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIEKAEEIRQSAPEKYVLLQQFDNPANPEIHEKTTGPE
IWNDTDGAVDVVVAGVGTGGTITGVSRYIKKAQGKAIVSVAVEPVDSPVISQTLAGQPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVETVSNEDAIEMAHRLMKEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDQEN
VQ

Sequences:

>Translated_322_residues
MSKIFEDNSYTIGNTPLVRLNRVSNGKVLAKVEARNPSFSVKCRIGANMIWDAEKRGVLTKDKELIEPTSGNTGIALAYV
AAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIEKAEEIRQSAPEKYVLLQQFDNPANPEIHEKTTGPE
IWNDTDGAVDVVVAGVGTGGTITGVSRYIKKAQGKAIVSVAVEPVDSPVISQTLAGQPVQPGPHKIQGIGAGFIPGNLDL
ELIDRVETVSNEDAIEMAHRLMKEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDQEN
VQ
>Mature_321_residues
SKIFEDNSYTIGNTPLVRLNRVSNGKVLAKVEARNPSFSVKCRIGANMIWDAEKRGVLTKDKELIEPTSGNTGIALAYVA
AARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGMKGAIEKAEEIRQSAPEKYVLLQQFDNPANPEIHEKTTGPEI
WNDTDGAVDVVVAGVGTGGTITGVSRYIKKAQGKAIVSVAVEPVDSPVISQTLAGQPVQPGPHKIQGIGAGFIPGNLDLE
LIDRVETVSNEDAIEMAHRLMKEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPSAAERYLSSVLFQGQFGDQENV
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=324, Percent_Identity=37.6543209876543, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI295821200, Length=324, Percent_Identity=37.6543209876543, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI4557415, Length=324, Percent_Identity=37.6543209876543, Blast_Score=185, Evalue=6e-47,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=76.875, Blast_Score=479, Evalue=1e-137,
Organism=Escherichia coli, GI2367138, Length=316, Percent_Identity=42.7215189873418, Blast_Score=208, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI17535051, Length=304, Percent_Identity=52.9605263157895, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=314, Percent_Identity=53.8216560509554, Blast_Score=277, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17562970, Length=312, Percent_Identity=49.3589743589744, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=50.1628664495114, Blast_Score=271, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17561720, Length=312, Percent_Identity=49.6794871794872, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=49.5454545454545, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=41.3249211356467, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=318, Percent_Identity=37.7358490566038, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17561716, Length=125, Percent_Identity=51.2, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=35.8730158730159, Blast_Score=169, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=334, Percent_Identity=32.9341317365269, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24643623, Length=307, Percent_Identity=37.4592833876222, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI20129101, Length=307, Percent_Identity=37.4592833876222, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34384; Mature: 34253

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFEDNSYTIGNTPLVRLNRVSNGKVLAKVEARNPSFSVKCRIGANMIWDAEKRGVLT
CCCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDKELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCH
KGAIEKAEEIRQSAPEKYVLLQQFDNPANPEIHEKTTGPEIWNDTDGAVDVVVAGVGTGG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYIKKAQGKAIVSVAVEPVDSPVISQTLAGQPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVETVSNEDAIEMAHRLMKEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPS
HHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCHHCCCCEEEEECC
AAERYLSSVLFQGQFGDQENVQ
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SKIFEDNSYTIGNTPLVRLNRVSNGKVLAKVEARNPSFSVKCRIGANMIWDAEKRGVLT
CCEECCCCEEECCCCEEEEEECCCCEEEEEEECCCCCEEEEEEECCCEEECCCCCCCCC
KDKELIEPTSGNTGIALAYVAAARGYKLTLTMPNTMSLERRKLLKALGANLVLTEGAKGM
CCHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCH
KGAIEKAEEIRQSAPEKYVLLQQFDNPANPEIHEKTTGPEIWNDTDGAVDVVVAGVGTGG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
TITGVSRYIKKAQGKAIVSVAVEPVDSPVISQTLAGQPVQPGPHKIQGIGAGFIPGNLDL
HHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCH
ELIDRVETVSNEDAIEMAHRLMKEEGILVGISSGAAVVAANRIAALPEFADKNIVVILPS
HHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCEEEEHHHHHHCCHHCCCCEEEEECC
AAERYLSSVLFQGQFGDQENVQ
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]