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The map label for this gene is nifS [H]

Identifier: 119774643

GI number: 119774643

Start: 1828047

End: 1830308

Strand: Reverse

Name: nifS [H]

Synonym: Sama_1506

Alternate gene names: 119774643

Gene position: 1830308-1828047 (Counterclockwise)

Preceding gene: 119774645

Following gene: 119774642

Centisome position: 42.5

GC content: 56.23

Gene sequence:

>2262_bases
ATGAGCCAGTCAAAGCCCTTGATCTATCTCGACGCCAACGCCACCACTCCCGTATTACCCGCCGCCGCCAAAGCCGCCAT
GGATGCCATGGAGCATATCTACGGGAATCCTTCCAGCAGCCATATCACGGGTCTTCAGGCCAAAGCCCTGATGGAACAAA
CCCGCGCCGCCGCCCGCAAACAGCTGGGTTCCGGCACAGGTAAACTGGTATTCACCAGCGGTGCAACCGAAGGCATTCAA
ACCGCTATTTTGTCGGCATTGGTGGCAGCCAGAGACACGGGCGTCCCTGCAGGCGCCTGCTTGCTGTATGGTGCCACCGA
GCACAAGGCTGTCCCCGAATCCCTCAAACACTGGAACAAGGTACTTGGCATCAATGCCGATGTGTTGGCGATTCCCGTGG
ATACTCAAGGCCGGTTGGATATGGCTTTTATCCGCGACAAGGCCCCCAACGCGCTGCTTATCTGTACTATGGCCGTGAAT
AACGAGACCGGTGTGTATCAGGATTTGGATGCCCTTTCACGTTGCATCAGACAAGCCAATCCTACCGTTTTCTGGATGGT
CGACTGTGTGCAGGCACTGGGAAAACTCGCTATCGATTTGGCCAGCAGCGGCATCGACTACGCGCCCTTCAGTGGCCATA
AACTCTATGCCCCCAAAGGCATTGGCATGCTCTATATCCGCGACGGTGCGCCCTTTACCCCATTTATCGCCGGTGGTGGT
CAGGAAGGTGGCTTGCGTTCAGGCACTGAAAACCTGCCGGCCATGGCAGCGCTCAAGGTCGTGCTGGATATGCTCAATGG
TGATGCTCAAGAGCGCTTTGAGTCCAAGGAAACCCTCAAGGGCTATCGCCTCAAGCTGCTTGCGGCACTTGAGCAAGCCT
TCGGTGAAGTGACGCTGAACCACAGCCTGGAAAACACGGTCCCTACCACCCTGAACTTTTCTATTGCGGGTTTTTCCAGC
AAGGAAATCATGGATCTCTTTGATGCCGCCGGGCTCCGTGTCAGCTCAGGCTCTGCCTGCAGCTCTAAAGTCACCCGCAG
TTTTGTGCTGGATGCCATGGGATTACCCCGTTGGCAGAGTGAATCGGCCATTCGCCTCTCATTTGGCCCGGCCATGACAG
CGGCCGAACTGGAGGCAGCCTGCGAACGTATCGTGACTGCCGCGTCCTCCCTGACCCATTCCTGTCTACTCACCCGAGAC
TCCCGCAGCACCGACGCAGACCGCGAGCCGCTGCAAGGCCTGGTGCAGTTTAAAGACGATGGCGCCTGTTGCTGGGTATA
CACAGACAAAGATAGCCGTGAAACCCTGATAGTTGACCCGCTGCCAGCGCTGAAACACCGTTTACTCAATCTGGTGCGTT
GCCAGGAACTCAAAGTCCTGGCAGTACTGGATACCCATGGCCATGGCGACCATCAGAGCATACGCAGCGAGCTCATCTGC
GAGCTTGGGCTAACAGACGCTTGCGACCATCTGGGATGGCCTTTAAGCAGCAAAGGCCAGATGTGGCGTGGTGAACAGGT
AAAGAGCCTGAGTGTCGGAAACAAGACATTGGTTTCACTGCCAAGCCCGGGTCACACAGACGACAGTCACAGCTTTATCA
TGCTGGATGATACACAGGCACCTCAATTTGCCTTTGTGGGCGATACTGTGTTGCCGGGCAGTATTGGCCGCAGCAACTTC
CCATCCTCTTCCAGTGTGGCACTCTTTCATACCCTGAAGCGACTGCATAGCCTGTTGGGAGACCAGAGCCTGCTGCTGTC
TTCCCATGACTATCACAACGATTTCTTCACCAATTTTGGCGTCGAACTCAAACAGTCCGCACTGCTTAACGACGTCCTTA
CCGGTGCCATGGATGAAGCCACCTTTGTTGCCCGTAAAGATGCCATGGATGCGACTCTCAAGGATGTGCAGGGTGAGACC
ATCATGTGCGGTGCCTATGGCCAGTGCCAACAGGCCAAAGGGGTGGAAGAGTATAGTGCTGACACTCTGGAGCAGTTGCT
TCACAGCAATCCCGAAGCCCTGGTATTGGACATTCGTGAACCTCACGAGTATCAGCTATCCCATCGAGAGAGCCGCTGCG
TTAACGTGCCCCTCACCCGCCTGGCGGGGTTTGTGGCGCGGGAGCCAGGAGCACGCAGTAAGCCTCTGGTATTGATTTGC
CGCAGTGGCAGCCGCTCGCTGGTGGCAGCAAAGGCTCTTGAGCGTTTTGGTTTCAGCGCTGTTGCCCACGTGGCAGGGGG
TTATGCCTTAAGTCAGTGCTGA

Upstream 100 bases:

>100_bases
ATAAGCCATCTTGTCGTTACACTAAAAACTGCTAATATAATCCATCAGAATTTCATATAACCAATAATTATAACAATTCA
ACACGAGCACAGAAAAAGCC

Downstream 100 bases:

>100_bases
GTGGGCCGATTGCCCTGATGGTAAGATAAACAGTAAAAAAGCGCCTGCGGGCGCTTTTTTACTTTTGGGGCATGAGCTAC
CGCCCACTGTTGCAATCAAG

Product: cysteine desulfurase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MSQSKPLIYLDANATTPVLPAAAKAAMDAMEHIYGNPSSSHITGLQAKALMEQTRAAARKQLGSGTGKLVFTSGATEGIQ
TAILSALVAARDTGVPAGACLLYGATEHKAVPESLKHWNKVLGINADVLAIPVDTQGRLDMAFIRDKAPNALLICTMAVN
NETGVYQDLDALSRCIRQANPTVFWMVDCVQALGKLAIDLASSGIDYAPFSGHKLYAPKGIGMLYIRDGAPFTPFIAGGG
QEGGLRSGTENLPAMAALKVVLDMLNGDAQERFESKETLKGYRLKLLAALEQAFGEVTLNHSLENTVPTTLNFSIAGFSS
KEIMDLFDAAGLRVSSGSACSSKVTRSFVLDAMGLPRWQSESAIRLSFGPAMTAAELEAACERIVTAASSLTHSCLLTRD
SRSTDADREPLQGLVQFKDDGACCWVYTDKDSRETLIVDPLPALKHRLLNLVRCQELKVLAVLDTHGHGDHQSIRSELIC
ELGLTDACDHLGWPLSSKGQMWRGEQVKSLSVGNKTLVSLPSPGHTDDSHSFIMLDDTQAPQFAFVGDTVLPGSIGRSNF
PSSSSVALFHTLKRLHSLLGDQSLLLSSHDYHNDFFTNFGVELKQSALLNDVLTGAMDEATFVARKDAMDATLKDVQGET
IMCGAYGQCQQAKGVEEYSADTLEQLLHSNPEALVLDIREPHEYQLSHRESRCVNVPLTRLAGFVAREPGARSKPLVLIC
RSGSRSLVAAKALERFGFSAVAHVAGGYALSQC

Sequences:

>Translated_753_residues
MSQSKPLIYLDANATTPVLPAAAKAAMDAMEHIYGNPSSSHITGLQAKALMEQTRAAARKQLGSGTGKLVFTSGATEGIQ
TAILSALVAARDTGVPAGACLLYGATEHKAVPESLKHWNKVLGINADVLAIPVDTQGRLDMAFIRDKAPNALLICTMAVN
NETGVYQDLDALSRCIRQANPTVFWMVDCVQALGKLAIDLASSGIDYAPFSGHKLYAPKGIGMLYIRDGAPFTPFIAGGG
QEGGLRSGTENLPAMAALKVVLDMLNGDAQERFESKETLKGYRLKLLAALEQAFGEVTLNHSLENTVPTTLNFSIAGFSS
KEIMDLFDAAGLRVSSGSACSSKVTRSFVLDAMGLPRWQSESAIRLSFGPAMTAAELEAACERIVTAASSLTHSCLLTRD
SRSTDADREPLQGLVQFKDDGACCWVYTDKDSRETLIVDPLPALKHRLLNLVRCQELKVLAVLDTHGHGDHQSIRSELIC
ELGLTDACDHLGWPLSSKGQMWRGEQVKSLSVGNKTLVSLPSPGHTDDSHSFIMLDDTQAPQFAFVGDTVLPGSIGRSNF
PSSSSVALFHTLKRLHSLLGDQSLLLSSHDYHNDFFTNFGVELKQSALLNDVLTGAMDEATFVARKDAMDATLKDVQGET
IMCGAYGQCQQAKGVEEYSADTLEQLLHSNPEALVLDIREPHEYQLSHRESRCVNVPLTRLAGFVAREPGARSKPLVLIC
RSGSRSLVAAKALERFGFSAVAHVAGGYALSQC
>Mature_752_residues
SQSKPLIYLDANATTPVLPAAAKAAMDAMEHIYGNPSSSHITGLQAKALMEQTRAAARKQLGSGTGKLVFTSGATEGIQT
AILSALVAARDTGVPAGACLLYGATEHKAVPESLKHWNKVLGINADVLAIPVDTQGRLDMAFIRDKAPNALLICTMAVNN
ETGVYQDLDALSRCIRQANPTVFWMVDCVQALGKLAIDLASSGIDYAPFSGHKLYAPKGIGMLYIRDGAPFTPFIAGGGQ
EGGLRSGTENLPAMAALKVVLDMLNGDAQERFESKETLKGYRLKLLAALEQAFGEVTLNHSLENTVPTTLNFSIAGFSSK
EIMDLFDAAGLRVSSGSACSSKVTRSFVLDAMGLPRWQSESAIRLSFGPAMTAAELEAACERIVTAASSLTHSCLLTRDS
RSTDADREPLQGLVQFKDDGACCWVYTDKDSRETLIVDPLPALKHRLLNLVRCQELKVLAVLDTHGHGDHQSIRSELICE
LGLTDACDHLGWPLSSKGQMWRGEQVKSLSVGNKTLVSLPSPGHTDDSHSFIMLDDTQAPQFAFVGDTVLPGSIGRSNFP
SSSSVALFHTLKRLHSLLGDQSLLLSSHDYHNDFFTNFGVELKQSALLNDVLTGAMDEATFVARKDAMDATLKDVQGETI
MCGAYGQCQQAKGVEEYSADTLEQLLHSNPEALVLDIREPHEYQLSHRESRCVNVPLTRLAGFVAREPGARSKPLVLICR
SGSRSLVAAKALERFGFSAVAHVAGGYALSQC

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=394, Percent_Identity=30.9644670050761, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=31.2649164677804, Blast_Score=160, Evalue=5e-39,
Organism=Homo sapiens, GI41327741, Length=213, Percent_Identity=29.5774647887324, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=35.4005167958656, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1787970, Length=376, Percent_Identity=24.468085106383, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789175, Length=397, Percent_Identity=25.1889168765743, Blast_Score=66, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI25143064, Length=376, Percent_Identity=30.3191489361702, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=26.605504587156, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319831, Length=389, Percent_Identity=32.133676092545, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI20129463, Length=396, Percent_Identity=31.8181818181818, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 80865; Mature: 80734

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00595 AA_TRANSFER_CLASS_5 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSKPLIYLDANATTPVLPAAAKAAMDAMEHIYGNPSSSHITGLQAKALMEQTRAAARK
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGSGTGKLVFTSGATEGIQTAILSALVAARDTGVPAGACLLYGATEHKAVPESLKHWNK
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCHHHHHHHHH
VLGINADVLAIPVDTQGRLDMAFIRDKAPNALLICTMAVNNETGVYQDLDALSRCIRQAN
HHCCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
PTVFWMVDCVQALGKLAIDLASSGIDYAPFSGHKLYAPKGIGMLYIRDGAPFTPFIAGGG
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCEEECCC
QEGGLRSGTENLPAMAALKVVLDMLNGDAQERFESKETLKGYRLKLLAALEQAFGEVTLN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
HSLENTVPTTLNFSIAGFSSKEIMDLFDAAGLRVSSGSACSSKVTRSFVLDAMGLPRWQS
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCC
ESAIRLSFGPAMTAAELEAACERIVTAASSLTHSCLLTRDSRSTDADREPLQGLVQFKDD
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHEECCC
GACCWVYTDKDSRETLIVDPLPALKHRLLNLVRCQELKVLAVLDTHGHGDHQSIRSELIC
CCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHH
ELGLTDACDHLGWPLSSKGQMWRGEQVKSLSVGNKTLVSLPSPGHTDDSHSFIMLDDTQA
HCCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCCC
PQFAFVGDTVLPGSIGRSNFPSSSSVALFHTLKRLHSLLGDQSLLLSSHDYHNDFFTNFG
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCC
VELKQSALLNDVLTGAMDEATFVARKDAMDATLKDVQGETIMCGAYGQCQQAKGVEEYSA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHHHHH
DTLEQLLHSNPEALVLDIREPHEYQLSHRESRCVNVPLTRLAGFVAREPGARSKPLVLIC
HHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEECCCHHHHHHHHHCCCCCCCCCEEEEE
RSGSRSLVAAKALERFGFSAVAHVAGGYALSQC
CCCCCHHHHHHHHHHHCHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
SQSKPLIYLDANATTPVLPAAAKAAMDAMEHIYGNPSSSHITGLQAKALMEQTRAAARK
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
QLGSGTGKLVFTSGATEGIQTAILSALVAARDTGVPAGACLLYGATEHKAVPESLKHWNK
HHCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHCCCHHHHHHHHH
VLGINADVLAIPVDTQGRLDMAFIRDKAPNALLICTMAVNNETGVYQDLDALSRCIRQAN
HHCCCCCEEEEECCCCCCEEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCC
PTVFWMVDCVQALGKLAIDLASSGIDYAPFSGHKLYAPKGIGMLYIRDGAPFTPFIAGGG
CCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEECCCCCCCEEECCC
QEGGLRSGTENLPAMAALKVVLDMLNGDAQERFESKETLKGYRLKLLAALEQAFGEVTLN
CCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEC
HSLENTVPTTLNFSIAGFSSKEIMDLFDAAGLRVSSGSACSSKVTRSFVLDAMGLPRWQS
CCCCCCCCCEEEEEEECCCHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHHHCCCCCCCC
ESAIRLSFGPAMTAAELEAACERIVTAASSLTHSCLLTRDSRSTDADREPLQGLVQFKDD
CCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHEECCC
GACCWVYTDKDSRETLIVDPLPALKHRLLNLVRCQELKVLAVLDTHGHGDHQSIRSELIC
CCEEEEEECCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHH
ELGLTDACDHLGWPLSSKGQMWRGEQVKSLSVGNKTLVSLPSPGHTDDSHSFIMLDDTQA
HCCCCHHHHHCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCCC
PQFAFVGDTVLPGSIGRSNFPSSSSVALFHTLKRLHSLLGDQSLLLSSHDYHNDFFTNFG
CCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHCC
VELKQSALLNDVLTGAMDEATFVARKDAMDATLKDVQGETIMCGAYGQCQQAKGVEEYSA
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCHHHHH
DTLEQLLHSNPEALVLDIREPHEYQLSHRESRCVNVPLTRLAGFVAREPGARSKPLVLIC
HHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCEECCCHHHHHHHHHCCCCCCCCCEEEEE
RSGSRSLVAAKALERFGFSAVAHVAGGYALSQC
CCCCCHHHHHHHHHHHCHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]