Click here to switch to the map view.

The map label for this gene is pepE

Identifier: 119774579

GI number: 119774579

Start: 1752234

End: 1752941

Strand: Reverse

Name: pepE

Synonym: Sama_1442

Alternate gene names: 119774579

Gene position: 1752941-1752234 (Counterclockwise)

Preceding gene: 119774584

Following gene: 119774574

Centisome position: 40.71

GC content: 56.64

Gene sequence:

>708_bases
ATGAACATCCGCGCCCTGCTGCTCAGCGCTTCCCGCGTTGGCGATACCCCATATCTTGAACACACTTTACCCTTTATCGC
TCCCCTGACGGAGCATGCCCGCAATTGGGTGTTTATTCCCTATGCCGGCATCAGCCTGGGGTATGATGTGTACCTTGAGA
AAGTTCGCGAGGGCCTGCGCAACCTGAATATCAACATCAGTGGTATCCATGAGCACGCTGACCCCCGTCAAGCGATTCGC
GATGCCGACGGCATTTTTGTTGGCGGCGGCAACACCTTCCATCTGCTGCACGAGCTGTACCGCTACGACTTGCTGTTTGT
CATTCGTGAGCAGGTAGAAGCCGGTAAGCCTTATGTAGGCTGGTCCGCTGGCTCAAACATCGCCGGTTTATCCATCCGGA
CCACCAACGACATGCCAATCATAGAGCCGCCCTCCTTCACCGCGCTGGGCTTGTTGCCGTTTCAGCTTAACCCCCATTAC
ACCGATTATCAGGCCCCGGGCCATAACGGTGAGACCCGCGCCCAGCGTCTGCTGGAGTTCACCATGGTCGACCCTCTGAC
CCCGGTTGTGGGCATTCAGGAAGGCAGTGCCCTGTATCGCCAAGGCGATAAGCTGACCTTATTGGGCGATAAAGAAGCCT
ACTTCTTCAAAGGCAGTGTGCAAAAATCGCCCATCGCTGCGGGCGCCGATCTGAGCGAGCTGCTGTAA

Upstream 100 bases:

>100_bases
CCATGAATCTTTGTTAACATTGGTCCACTCAAGCCCATCACGTCTTATTTTTAAACGGGCATACCGATCTAATAACGACC
TCTGAAAATCAGGAAATCCC

Downstream 100 bases:

>100_bases
TCGACTGTAAATAATAAAGGTCGCCTAACGGTGACCTTTATTATTTCTTGCTTAACTGACTTTTGCGGCTTGGCTTATCA
AACACACCTTGGCGTCAGTC

Product: peptidase E

Products: NA

Alternate protein names: Alpha-aspartyl dipeptidase; Asp-specific dipeptidase; Dipeptidase E

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MNIRALLLSASRVGDTPYLEHTLPFIAPLTEHARNWVFIPYAGISLGYDVYLEKVREGLRNLNINISGIHEHADPRQAIR
DADGIFVGGGNTFHLLHELYRYDLLFVIREQVEAGKPYVGWSAGSNIAGLSIRTTNDMPIIEPPSFTALGLLPFQLNPHY
TDYQAPGHNGETRAQRLLEFTMVDPLTPVVGIQEGSALYRQGDKLTLLGDKEAYFFKGSVQKSPIAAGADLSELL

Sequences:

>Translated_235_residues
MNIRALLLSASRVGDTPYLEHTLPFIAPLTEHARNWVFIPYAGISLGYDVYLEKVREGLRNLNINISGIHEHADPRQAIR
DADGIFVGGGNTFHLLHELYRYDLLFVIREQVEAGKPYVGWSAGSNIAGLSIRTTNDMPIIEPPSFTALGLLPFQLNPHY
TDYQAPGHNGETRAQRLLEFTMVDPLTPVVGIQEGSALYRQGDKLTLLGDKEAYFFKGSVQKSPIAAGADLSELL
>Mature_235_residues
MNIRALLLSASRVGDTPYLEHTLPFIAPLTEHARNWVFIPYAGISLGYDVYLEKVREGLRNLNINISGIHEHADPRQAIR
DADGIFVGGGNTFHLLHELYRYDLLFVIREQVEAGKPYVGWSAGSNIAGLSIRTTNDMPIIEPPSFTALGLLPFQLNPHY
TDYQAPGHNGETRAQRLLEFTMVDPLTPVVGIQEGSALYRQGDKLTLLGDKEAYFFKGSVQKSPIAAGADLSELL

Specific function: Hydrolyzes dipeptides containing N-terminal aspartate residues. May play a role in allowing the cell to use peptide aspartate to spare carbon otherwise required for the synthesis of the aspartate family of amino acids

COG id: COG3340

COG function: function code E; Peptidase E

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S51 family

Homologues:

Organism=Escherichia coli, GI1790452, Length=223, Percent_Identity=43.4977578475336, Blast_Score=187, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24641669, Length=222, Percent_Identity=47.7477477477478, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPE_SHEAM (A1S5J3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927319.1
- ProteinModelPortal:   A1S5J3
- SMR:   A1S5J3
- STRING:   A1S5J3
- MEROPS:   S51.001
- GeneID:   4603694
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1442
- NMPDR:   fig|326297.7.peg.1360
- eggNOG:   COG3340
- HOGENOM:   HBG298965
- OMA:   ALWRQGD
- PhylomeDB:   A1S5J3
- ProtClustDB:   PRK05282
- GO:   GO:0005737
- GO:   GO:0006508
- HAMAP:   MF_00510
- InterPro:   IPR005320
- InterPro:   IPR023172

Pfam domain/function: PF03575 Peptidase_S51

EC number: =3.4.13.21

Molecular weight: Translated: 26018; Mature: 26018

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: ACT_SITE 122-122 ACT_SITE 137-137 ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRALLLSASRVGDTPYLEHTLPFIAPLTEHARNWVFIPYAGISLGYDVYLEKVREGLR
CCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
NLNINISGIHEHADPRQAIRDADGIFVGGGNTFHLLHELYRYDLLFVIREQVEAGKPYVG
HCCCEEEEECCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WSAGSNIAGLSIRTTNDMPIIEPPSFTALGLLPFQLNPHYTDYQAPGHNGETRAQRLLEF
ECCCCCEEEEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TMVDPLTPVVGIQEGSALYRQGDKLTLLGDKEAYFFKGSVQKSPIAAGADLSELL
HHCCCCCCEEECCCCCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHC
>Mature Secondary Structure
MNIRALLLSASRVGDTPYLEHTLPFIAPLTEHARNWVFIPYAGISLGYDVYLEKVREGLR
CCCEEEEEEHHHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
NLNINISGIHEHADPRQAIRDADGIFVGGGNTFHLLHELYRYDLLFVIREQVEAGKPYVG
HCCCEEEEECCCCCHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
WSAGSNIAGLSIRTTNDMPIIEPPSFTALGLLPFQLNPHYTDYQAPGHNGETRAQRLLEF
ECCCCCEEEEEEEECCCCCEECCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
TMVDPLTPVVGIQEGSALYRQGDKLTLLGDKEAYFFKGSVQKSPIAAGADLSELL
HHCCCCCCEEECCCCCHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA