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The map label for this gene is bioB [H]

Identifier: 119774574

GI number: 119774574

Start: 1747044

End: 1748354

Strand: Reverse

Name: bioB [H]

Synonym: Sama_1437

Alternate gene names: 119774574

Gene position: 1748354-1747044 (Counterclockwise)

Preceding gene: 119774579

Following gene: 119774571

Centisome position: 40.6

GC content: 57.82

Gene sequence:

>1311_bases
ATGAGCATAGATTTTGAGTTTGACCGTAACCACCTTTGGCACCCTTATACCTCCATGGCAGCGCCTTTACCTGTGCTGGG
TGTGCAAAGTGCCCGAGGCTGCCGCCTGACACTGGAGGATGGTCGCGAGTTGGTTGACGGCACCTCTTCCTGGTGGTCGG
CCATCCATGGCTATGGGCATCCGGCGCTGGTAAGCGCGGTGCAGGCTCAGGCCGCTAAACTCAGCCATGTGATGTTCGGC
GGCCTCACCCATGAGCCTGCCACCGCGCTAGGTAAAGCACTGCTTAAGCTGGTTCACCCCACACTGACCAGGGTGTTTTA
TGCCGACTCTGGCTCAGTGGCGGTGGAAGTCGCACTCAAGATGGCCATGCAGTACTGGCAAGGGAGAAGCGAGCCGCAAA
AGCACAAACTGCTGACCGTACTCGGCGGCTATCATGGCGATACCTTTGCGGCCATGAGTGTGTGCGATCCCAACTCAGGC
ATGCACGGCATGTTTGGCCATCTGGTACCCAAGCAAATATTCGCCAAGGCGCCTCCAGCAGGTTTTGGTCGTTCCCTTGC
AGAGCAAGACCTGGCAGAACTGCGCGCATTGCTTCAGGCACATCACCAGGAAATTGCAGCGCTTATCATTGAACCCATCA
TGCAGGGAGCCGGCGGCCTGAGATTCCATAGCCCTGACTATCTCAAATCCGTGCGTGCGCTCTGTGACGAATATAGGGTG
CTGCTGATTTTGGATGAGATTGCCACCGGTTTTGGCCGCACCGGCAAGGCATTCGCCTACGAGCATGCGGGTATAACCCC
TGATTTGCTCTGCCTTGGCAAGGCGCTCACCGGCGGCATGCTGAGTCTCGCTGCAACCCTGTGCACGGATGAGGTGGCAG
CTGGCATTTCGAGTTCACCCGCCGGTGTATTCATGCACGGCCCCACCTTTATGGCTAATCCGCTCGCCTGCGCGGCCGCT
CTGGCAAGCCTTGAACTTTTTGCCGAAAACCGCTGGCCAGCGCAGGTTGCCAATATTGAAGCCAAGCTGACCGAGTTACT
GGAAACCGCCAGAACGCTGCCGCAGGTAGCCGATGTACGAGTGCTCGGCGCCGTGGGTGTGATTGAAATGGTGCAAACCG
TCGACACGGCCTTTGCACTTATGGCCTTTGCCGCGCGAGGAGTGTGGGTTCGCCCCTTCGGCAGATTAATTTACGTGATG
CCGCCCTACTGTATCAGTGATTCAGAGCTAGGCATGCTGACCCGTGCCATGGTGGAGGTTGCTTCGCTGGAAGGCATCTG
CAAAACCGCCAACAACCCGGCGCACGGATAA

Upstream 100 bases:

>100_bases
CAATTAATGTTTTCTGTTGGCTAGGATAACCCTGGCCCTTACACTGTCAACGCCAACCAGTTGGCAGGGTTTACTGATGA
TTAAATTTTGAGCGCGCAAA

Downstream 100 bases:

>100_bases
CGCGAGTTCAGGGCTGAAATGTCCTGGCCATGCGCCTGATGGCGACCAGTGGTACGCCGTGGCTGTTTCGCTCGGCACAG
AGCGCCTGATGGCGCCTGTC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGHPALVSAVQAQAAKLSHVMFG
GLTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALKMAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSG
MHGMFGHLVPKQIFAKAPPAGFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRV
LLILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSPAGVFMHGPTFMANPLACAAA
LASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVRVLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVM
PPYCISDSELGMLTRAMVEVASLEGICKTANNPAHG

Sequences:

>Translated_436_residues
MSIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGHPALVSAVQAQAAKLSHVMFG
GLTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALKMAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSG
MHGMFGHLVPKQIFAKAPPAGFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRV
LLILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSPAGVFMHGPTFMANPLACAAA
LASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVRVLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVM
PPYCISDSELGMLTRAMVEVASLEGICKTANNPAHG
>Mature_435_residues
SIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGHPALVSAVQAQAAKLSHVMFGG
LTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALKMAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSGM
HGMFGHLVPKQIFAKAPPAGFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRVL
LILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSPAGVFMHGPTFMANPLACAAAL
ASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVRVLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVMP
PYCISDSELGMLTRAMVEVASLEGICKTANNPAHG

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=428, Percent_Identity=30.3738317757009, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI284507298, Length=321, Percent_Identity=32.0872274143302, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI37574042, Length=411, Percent_Identity=23.6009732360097, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI226442709, Length=393, Percent_Identity=23.9185750636132, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI13994255, Length=363, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI226442705, Length=366, Percent_Identity=23.7704918032787, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI188536080, Length=292, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI38679950, Length=292, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI38679946, Length=292, Percent_Identity=25, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI24119277, Length=440, Percent_Identity=22.9545454545455, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786991, Length=414, Percent_Identity=57.0048309178744, Blast_Score=466, Evalue=1e-133,
Organism=Escherichia coli, GI145693181, Length=388, Percent_Identity=34.7938144329897, Blast_Score=179, Evalue=4e-46,
Organism=Escherichia coli, GI1789016, Length=399, Percent_Identity=31.328320802005, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789759, Length=352, Percent_Identity=33.5227272727273, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1788044, Length=398, Percent_Identity=32.6633165829146, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1786349, Length=332, Percent_Identity=31.9277108433735, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI1787560, Length=347, Percent_Identity=28.2420749279539, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25144271, Length=415, Percent_Identity=29.3975903614458, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25144274, Length=273, Percent_Identity=32.6007326007326, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71992977, Length=409, Percent_Identity=24.6943765281174, Blast_Score=83, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324386, Length=456, Percent_Identity=42.9824561403509, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6323470, Length=420, Percent_Identity=29.7619047619048, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324432, Length=354, Percent_Identity=29.6610169491525, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21357415, Length=399, Percent_Identity=30.5764411027569, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI28574759, Length=406, Percent_Identity=25.1231527093596, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI161085790, Length=416, Percent_Identity=25, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356575, Length=364, Percent_Identity=26.0989010989011, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281366494, Length=315, Percent_Identity=24.1269841269841, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667139, Length=315, Percent_Identity=24.1269841269841, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667143, Length=315, Percent_Identity=24.1269841269841, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46672; Mature: 46540

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGH
CCCCCEECCCCCCCCHHHHCCCCCCEECCCCCCCEEEHHCCHHHHCCCHHHHHHHHCCCC
PALVSAVQAQAAKLSHVMFGGLTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
MAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSGMHGMFGHLVPKQIFAKAPPA
HHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
GFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCEE
LLILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSP
EEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGVFMHGPTFMANPLACAAALASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVR
CEEEEECCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
VLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVMPPYCISDSELGMLTRAMVEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCHHHHHHHHHHHH
ASLEGICKTANNPAHG
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIDFEFDRNHLWHPYTSMAAPLPVLGVQSARGCRLTLEDGRELVDGTSSWWSAIHGYGH
CCCCEECCCCCCCCHHHHCCCCCCEECCCCCCCEEEHHCCHHHHCCCHHHHHHHHCCCC
PALVSAVQAQAAKLSHVMFGGLTHEPATALGKALLKLVHPTLTRVFYADSGSVAVEVALK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHH
MAMQYWQGRSEPQKHKLLTVLGGYHGDTFAAMSVCDPNSGMHGMFGHLVPKQIFAKAPPA
HHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCC
GFGRSLAEQDLAELRALLQAHHQEIAALIIEPIMQGAGGLRFHSPDYLKSVRALCDEYRV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCEE
LLILDEIATGFGRTGKAFAYEHAGITPDLLCLGKALTGGMLSLAATLCTDEVAAGISSSP
EEEEHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AGVFMHGPTFMANPLACAAALASLELFAENRWPAQVANIEAKLTELLETARTLPQVADVR
CEEEEECCCHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHH
VLGAVGVIEMVQTVDTAFALMAFAARGVWVRPFGRLIYVMPPYCISDSELGMLTRAMVEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCEEEECCCCCCCCCHHHHHHHHHHHH
ASLEGICKTANNPAHG
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA