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The map label for this gene is polB [H]

Identifier: 119774387

GI number: 119774387

Start: 1533668

End: 1536073

Strand: Direct

Name: polB [H]

Synonym: Sama_1250

Alternate gene names: 119774387

Gene position: 1533668-1536073 (Clockwise)

Preceding gene: 119774380

Following gene: 119774388

Centisome position: 35.62

GC content: 52.95

Gene sequence:

>2406_bases
ATGGAATTGTCGTCCCCTTGCTACCCTGGCTTCAATGAATCACTGGCTCCGATACTGCTCAGGGGGCGGGTGCTCACGCG
CGCCAATGTGCAGCGCAGCGGTGACACTGTGCTGGAATATCTGATTAAAACCGTCAACGGGCCGGTGAAGGTGGAAGTGC
ATAATCAAAGACCCATGGCCTTTTGCCATAGCGATGATGTAGAGCGCTTGCTCGTGGAGGGCGCTGAAACTGAAGTCTGG
TCGCACCCACTCGAGCTGCAAAGTTTCAGCAGGCAACCCGTGCATGCAATCTATTGCCGTACAGGGCTTATGCTCAAGCG
CCTCGTAAATCGAGCGCAGGAGTTGGGTATTGCGCTTTATGAGGCCGATATCAAACCAGAGCAGCGCTTCCTGATTGAAC
GTTTTGTGGCCCTTGATGCCGAGTTTTTCGGCCACTTCGACTCGAGCGGCCCAAGCCGGTTTGTTGCCAGCCGGGTGAGG
GCAACCAGTCAACACATTCCCCTGAAAGCGGTTTCCCTCGATATCGAATGCAGTATGTCGGGGGAGCTCTATTCCATTGG
TTTATATGGTGACTGCGAACCCAAAGTCATCATAGTCGGTGAGGGGGAAACCGAGCTCACTTGCCATCGGGATGGGGTGG
AACATAAGGTCGCCGTGGAGTGGGCTTGTGATGAAGTGGCCCTGCTGTTATCGCTGCAGCGCTGGTTTATGGAGGTTGAC
CCCGACATCCTCTTGGGCTGGGCTGTGGTGACTTTCGATCTGTCGCTCCTGTGGCGTAGGGCAAAACACCATGGTATGCG
GCTTTGCATCGGGCGGTTCGGTAAACCACTCGCTTGGAAAGTAGAAGACAAGCACAGACCGGAGACCCTGGACTTGTCCG
GACGGGTAGTTCTCGATGGTATTGATTGGTTAAAGGCCGCATTTTACCAGTTTGACAGTTTCGCCTTGGACAGGGTGGCG
GGTGAACTCCTGGGGGAGGGCAAAGCTATCCATAATCCTGATGACCGTGGCGAAGAAATCACCCGGTTGTTTCGTGAAGA
CAAAGCTGCTTTGGCCTATTACAACCTCACCGACTGCCGCCTGGTGTGGGATATTTTTCAAAAGACCGATCTCATCCATT
TTGCCCTTGAGCGGGCGCGCCTCACTGGATTGGAATTTGGTAGGGTGGGTGCCAGTATTGCGGCTTTTAATCACCTTTAT
CTGCCCCATTTGCACCGGGCGGGTTTTGTGGCACCGGCAATGCAAACAGTCCCCGGGCCCGATAGCCCGGGTGGCTATGT
GATGGATTCGGTTCCGGGCTTATATCGCAACGTATTGGTGCTGGATTACAAGAGCCTGTATCCCTCCATCATCCGAACCT
TTTTAATCGACCCCAAGGGGCTCGTGCTCGGTCTATCTGAACCTGAGGCCATGTCTGTTGAAGGATTTCGCGGCGCGAGA
TTCAGTCGACAGTCGCCCATTTTGCCCAAACTTGTTGCAACACTGGCGAGTCGTAGAGAAGAGGCAAAAGCTAACCAGAA
CGGCCCCATGTCTCAGGCGGTGAAAATCATCATGAACTCTCTCTATGGGGTGCTGGGTTCCCGTGGATGTGTATTTCACG
ATTATCGTTTGGCAAGCTCCATTACCCTGCGGGGGCATGAGATCATGCGCACAACCAAGGCCTGGATAGAAGCGGAAGGA
TTTCAGGTCATCTATGGCGACACCGACTCTACCTTCGTTTGGTTGGGTGACGAGGCCGGGAATGCAAAGGCCACGGGGCG
TGCGCTTGTGGACATGGTGAATCAGCGGTGGCGTGAGCAGCTGCGAGAGGATAAAGGACTTGAGTGCTTTTTGGAGCTTG
AGTTTGAACGGCACTTCGAGCAATTTTTTATGCCCACACTCAGACACTCCACCGAAGGAAGCAAAAAACGCTATGTTGGG
CTGTACGACAATGGCAATGAAAAGGTACTTGTGTTCAAGGGAATGGAGCAGGTTAGAAGTGACTGGAGTCCGCTGGCTCG
CCGGGTTCAATACGAACTTTACCGGCGGTTATTCCAGCAGGAAAACTTGACTGAGTATGTGCAGGAAGTAGAGAAGTCGT
TGTATTCCGGAGAGCTCGATGATGAGCTGGTGTTCCATAAACGTCTCAAGCGTGATATTAGCCAGTACACGGCAAAATCC
GCTCCCCACGTGAAAGCCGCTGCCTTGCTTGTCAGTGCAACGGGTGATGCCAACCGGGGAAAGCGTGGTGCAGCTATCAG
GTACCTCTTTACCAAAGCGGGGGCTGAGCCCTTGGGTTTTCAGTCTCAGCCAATTGATTATGACTACTACTACGACCGCC
AATTGTTACCCATTTTGGAACCCATACTCGCCGTTTTGAAAATTAATTACCAAAAATCAGGCGCTGAGCAATTGTTGTTA
ATCTAG

Upstream 100 bases:

>100_bases
GGGCTCTCGGCAGCTCAATTATGCTGTACATAATAACAGTGATTGCTTAGGCTAAGAACCCTGCAATCATTGCTTATGTG
TGTTTTTTACTCCTTTACCC

Downstream 100 bases:

>100_bases
TATGTCGCCATGCAATCTTGAGTGTTTTAGGTTAATAGTCGGTTAATTGCTTATCTGATAGAGATTTCAATTGATGGCTG
TTGTGATTTTTAACACGTTT

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMAFCHSDDVERLLVEGAETEVW
SHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALYEADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVR
ATSQHIPLKAVSLDIECSMSGELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDGIDWLKAAFYQFDSFALDRVA
GELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCRLVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLY
LPHLHRAGFVAPAMQTVPGPDSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASSITLRGHEIMRTTKAWIEAEG
FQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQLREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVG
LYDNGNEKVLVFKGMEQVRSDWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILEPILAVLKINYQKSGAEQLLL
I

Sequences:

>Translated_801_residues
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMAFCHSDDVERLLVEGAETEVW
SHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALYEADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVR
ATSQHIPLKAVSLDIECSMSGELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDGIDWLKAAFYQFDSFALDRVA
GELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCRLVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLY
LPHLHRAGFVAPAMQTVPGPDSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASSITLRGHEIMRTTKAWIEAEG
FQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQLREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVG
LYDNGNEKVLVFKGMEQVRSDWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILEPILAVLKINYQKSGAEQLLL
I
>Mature_801_residues
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMAFCHSDDVERLLVEGAETEVW
SHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALYEADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVR
ATSQHIPLKAVSLDIECSMSGELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDGIDWLKAAFYQFDSFALDRVA
GELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCRLVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLY
LPHLHRAGFVAPAMQTVPGPDSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASSITLRGHEIMRTTKAWIEAEG
FQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQLREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVG
LYDNGNEKVLVFKGMEQVRSDWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILEPILAVLKINYQKSGAEQLLL
I

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=699, Percent_Identity=26.4663805436338, Blast_Score=146, Evalue=1e-34,
Organism=Homo sapiens, GI106507301, Length=662, Percent_Identity=23.4138972809668, Blast_Score=110, Evalue=6e-24,
Organism=Escherichia coli, GI1786246, Length=795, Percent_Identity=46.5408805031447, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=701, Percent_Identity=26.5335235378031, Blast_Score=136, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32565317, Length=634, Percent_Identity=23.6593059936909, Blast_Score=111, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324227, Length=444, Percent_Identity=26.5765765765766, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320101, Length=703, Percent_Identity=24.4665718349929, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6325090, Length=726, Percent_Identity=22.4517906336088, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24664937, Length=745, Percent_Identity=25.9060402684564, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24648780, Length=524, Percent_Identity=22.1374045801527, Blast_Score=87, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90753; Mature: 90753

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMA
CCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
FCHSDDVERLLVEGAETEVWSHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALY
EECCCHHHHHHHCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
EADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVRATSQHIPLKAVSLDIECSMS
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECC
GELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
CCEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDG
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCEEEEECCCCCCCEECCCCEEEECC
IDWLKAAFYQFDSFALDRVAGELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHH
LVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLYLPHLHRAGFVAPAMQTVPGP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
DSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
CCCCCEEECCCCHHHHHHHEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCE
ITLRGHEIMRTTKAWIEAEGFQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQ
EEECCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVGLYDNGNEKVLVFKGMEQVRS
HHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCHHHHHC
DWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILE
CCCCEEEEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEECCCHHHHHH
PILAVLKINYQKSGAEQLLLI
HHHHHHEECCCCCCCCCEEEC
>Mature Secondary Structure
MELSSPCYPGFNESLAPILLRGRVLTRANVQRSGDTVLEYLIKTVNGPVKVEVHNQRPMA
CCCCCCCCCCCCCCHHHHHHHCCHHHHCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCEE
FCHSDDVERLLVEGAETEVWSHPLELQSFSRQPVHAIYCRTGLMLKRLVNRAQELGIALY
EECCCHHHHHHHCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
EADIKPEQRFLIERFVALDAEFFGHFDSSGPSRFVASRVRATSQHIPLKAVSLDIECSMS
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEECC
GELYSIGLYGDCEPKVIIVGEGETELTCHRDGVEHKVAVEWACDEVALLLSLQRWFMEVD
CCEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHCC
PDILLGWAVVTFDLSLLWRRAKHHGMRLCIGRFGKPLAWKVEDKHRPETLDLSGRVVLDG
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHCCCCEEEEECCCCCCCEECCCCEEEECC
IDWLKAAFYQFDSFALDRVAGELLGEGKAIHNPDDRGEEITRLFREDKAALAYYNLTDCR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCHHH
LVWDIFQKTDLIHFALERARLTGLEFGRVGASIAAFNHLYLPHLHRAGFVAPAMQTVPGP
HHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCC
DSPGGYVMDSVPGLYRNVLVLDYKSLYPSIIRTFLIDPKGLVLGLSEPEAMSVEGFRGAR
CCCCCEEECCCCHHHHHHHEEEHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCC
FSRQSPILPKLVATLASRREEAKANQNGPMSQAVKIIMNSLYGVLGSRGCVFHDYRLASS
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCE
ITLRGHEIMRTTKAWIEAEGFQVIYGDTDSTFVWLGDEAGNAKATGRALVDMVNQRWREQ
EEECCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LREDKGLECFLELEFERHFEQFFMPTLRHSTEGSKKRYVGLYDNGNEKVLVFKGMEQVRS
HHCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEECCHHHHHC
DWSPLARRVQYELYRRLFQQENLTEYVQEVEKSLYSGELDDELVFHKRLKRDISQYTAKS
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
APHVKAAALLVSATGDANRGKRGAAIRYLFTKAGAEPLGFQSQPIDYDYYYDRQLLPILE
CCCCEEEEEEEEECCCCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCEECCCHHHHHH
PILAVLKINYQKSGAEQLLLI
HHHHHHEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]