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The map label for this gene is mdeA [H]

Identifier: 119774379

GI number: 119774379

Start: 1526273

End: 1527451

Strand: Direct

Name: mdeA [H]

Synonym: Sama_1242

Alternate gene names: 119774379

Gene position: 1526273-1527451 (Clockwise)

Preceding gene: 119774378

Following gene: 119774380

Centisome position: 35.44

GC content: 54.54

Gene sequence:

>1179_bases
ATGCAGGAGAAAAGCAAGTTAGCCACCCTCGTCGTTCATGGTGGCCACGAGCGTGATGCCATGGGGGCGCTGGTGAGTCC
CCTCTATCAGAGTGCGACCTTTGTATTCGACAACGCCCGTCAGGGTGGTGCCCGTTTTGCCGGTGATGAGGCTGGCTATA
TCTACACCCGACTGGGTAATCCCACGACAGCTGAGCTTGAGCGCAAGCTGGCCATTCTAGAAGGGGCCGAGGAAGCAGCA
GCTACGGCGTCCGGTATGGGCGCTGTGTCAGCGGCGCTGCTTGCCAATCTGAGCCAGGGCGATCATCTGGTGGCGAGCCG
GGCCGTGTATGGCTGTACCTTTGCCCTGATGACTGATCTGATGGCCCGTTTTGGGATTGAAGTAACTCTGGTGGATTTTA
AAGAGCCTGCCGCCATCGAAGCCGCTATCCGTGACAATACCCGGGCGATTTTCTGTGAAACCCCGGTGAATCCCCATCTG
GATGTATTCGACCTCGATGCCATTGCTGCCATCGGCAAGAGACACGGTTTGCTTACCATTGTCGATAACACCTTTATGAC
TCCGCTGCTGCAGCGCCCGCTGGACCACGGCATTGATATGGTCATTCACAGCGCCACCAAGTACCTCAATGGTCATGGCG
ATGTGATTGCCGGCATGGTGGCAGGCAGCAAGGAACAGATTGATAAAGTGAAATATCAAATCATCAAGGATATAGGCGCT
GTGATGTCGCCCCATGATGCCTGGTTGATACTGAGGGGCATGAAAACCCTAGATGTGCGTGTGCAGCGCCACTGTGATAA
TGCGGAAAAAATCGCGGATTTCCTTGAGGCTCACCCCAGGGTAGGGCGAGTCTACTATCCGGCGCTTAAGAGCCATCAGG
GACACAGGTTCCTGGGAACGCAGATGCGCCGCGCCGGGGGCGTTATTGCCTTTGAACTTAAGTCGGATATCGAAGGTTCC
ATCAATTTTGTCGACAGTCTCAAGGTGTTCACCATCGCTGTAAGCCTGGGGGATGCCGAGTCCCTTATTCAACACCCGGC
ATCCATGACGCACTCACCATACACCCCCGAAGCGCGGCTTGAGGCGGGGATTACAGATACCTTGCTGAGAATTTCGGTAG
GACTGGAAGATGTGGATGATTTGATTGCGGATTTATCTCAGGCTTTGGCAAAAATCTAA

Upstream 100 bases:

>100_bases
GCTGCGGCCCTTGGCGTTTGCGCCCGGGGCAGTCATGTTAGTGCCCACACCACCGCCACAACCTATAATTTCTTAAGGTG
GTGCTTTTGGGAGAGCAGTT

Downstream 100 bases:

>100_bases
CCGGAGATTTGCGCTTTTACAAAGGGTAACTAGAGTTATTGGAGTGGCAAGGAAAGGGAGCCCCGGCCACGTTAAACATC
TCAATGGAGTGAACCTATGA

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MQEKSKLATLVVHGGHERDAMGALVSPLYQSATFVFDNARQGGARFAGDEAGYIYTRLGNPTTAELERKLAILEGAEEAA
ATASGMGAVSAALLANLSQGDHLVASRAVYGCTFALMTDLMARFGIEVTLVDFKEPAAIEAAIRDNTRAIFCETPVNPHL
DVFDLDAIAAIGKRHGLLTIVDNTFMTPLLQRPLDHGIDMVIHSATKYLNGHGDVIAGMVAGSKEQIDKVKYQIIKDIGA
VMSPHDAWLILRGMKTLDVRVQRHCDNAEKIADFLEAHPRVGRVYYPALKSHQGHRFLGTQMRRAGGVIAFELKSDIEGS
INFVDSLKVFTIAVSLGDAESLIQHPASMTHSPYTPEARLEAGITDTLLRISVGLEDVDDLIADLSQALAKI

Sequences:

>Translated_392_residues
MQEKSKLATLVVHGGHERDAMGALVSPLYQSATFVFDNARQGGARFAGDEAGYIYTRLGNPTTAELERKLAILEGAEEAA
ATASGMGAVSAALLANLSQGDHLVASRAVYGCTFALMTDLMARFGIEVTLVDFKEPAAIEAAIRDNTRAIFCETPVNPHL
DVFDLDAIAAIGKRHGLLTIVDNTFMTPLLQRPLDHGIDMVIHSATKYLNGHGDVIAGMVAGSKEQIDKVKYQIIKDIGA
VMSPHDAWLILRGMKTLDVRVQRHCDNAEKIADFLEAHPRVGRVYYPALKSHQGHRFLGTQMRRAGGVIAFELKSDIEGS
INFVDSLKVFTIAVSLGDAESLIQHPASMTHSPYTPEARLEAGITDTLLRISVGLEDVDDLIADLSQALAKI
>Mature_392_residues
MQEKSKLATLVVHGGHERDAMGALVSPLYQSATFVFDNARQGGARFAGDEAGYIYTRLGNPTTAELERKLAILEGAEEAA
ATASGMGAVSAALLANLSQGDHLVASRAVYGCTFALMTDLMARFGIEVTLVDFKEPAAIEAAIRDNTRAIFCETPVNPHL
DVFDLDAIAAIGKRHGLLTIVDNTFMTPLLQRPLDHGIDMVIHSATKYLNGHGDVIAGMVAGSKEQIDKVKYQIIKDIGA
VMSPHDAWLILRGMKTLDVRVQRHCDNAEKIADFLEAHPRVGRVYYPALKSHQGHRFLGTQMRRAGGVIAFELKSDIEGS
INFVDSLKVFTIAVSLGDAESLIQHPASMTHSPYTPEARLEAGITDTLLRISVGLEDVDDLIADLSQALAKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=386, Percent_Identity=40.1554404145078, Blast_Score=256, Evalue=4e-68,
Organism=Homo sapiens, GI299473759, Length=386, Percent_Identity=37.5647668393782, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI25453487, Length=385, Percent_Identity=35.5844155844156, Blast_Score=197, Evalue=2e-50,
Organism=Escherichia coli, GI1790375, Length=385, Percent_Identity=38.7012987012987, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1789383, Length=397, Percent_Identity=31.2342569269521, Blast_Score=188, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=374, Percent_Identity=40.3743315508021, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17560056, Length=388, Percent_Identity=43.0412371134021, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI17538055, Length=388, Percent_Identity=41.2371134020619, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=399, Percent_Identity=39.8496240601504, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=31.8807339449541, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321254, Length=410, Percent_Identity=31.219512195122, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=330, Percent_Identity=31.2121212121212, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321904, Length=406, Percent_Identity=29.3103448275862, Blast_Score=139, Evalue=7e-34,
Organism=Drosophila melanogaster, GI19922554, Length=391, Percent_Identity=40.4092071611253, Blast_Score=276, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42370; Mature: 42370

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKSKLATLVVHGGHERDAMGALVSPLYQSATFVFDNARQGGARFAGDEAGYIYTRLGN
CCCHHCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCC
PTTAELERKLAILEGAEEAAATASGMGAVSAALLANLSQGDHLVASRAVYGCTFALMTDL
CCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MARFGIEVTLVDFKEPAAIEAAIRDNTRAIFCETPVNPHLDVFDLDAIAAIGKRHGLLTI
HHHHCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCEECHHHHHHHHHCCCEEEE
VDNTFMTPLLQRPLDHGIDMVIHSATKYLNGHGDVIAGMVAGSKEQIDKVKYQIIKDIGA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
VMSPHDAWLILRGMKTLDVRVQRHCDNAEKIADFLEAHPRVGRVYYPALKSHQGHRFLGT
HCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECHHHCCCCCHHHHH
QMRRAGGVIAFELKSDIEGSINFVDSLKVFTIAVSLGDAESLIQHPASMTHSPYTPEARL
HHHHHCCEEEEEECCCCCCCCHHHHCEEEEEEEEECCCHHHHHHCCHHCCCCCCCCCHHH
EAGITDTLLRISVGLEDVDDLIADLSQALAKI
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQEKSKLATLVVHGGHERDAMGALVSPLYQSATFVFDNARQGGARFAGDEAGYIYTRLGN
CCCHHCEEEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCEEEEECCC
PTTAELERKLAILEGAEEAAATASGMGAVSAALLANLSQGDHLVASRAVYGCTFALMTDL
CCHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MARFGIEVTLVDFKEPAAIEAAIRDNTRAIFCETPVNPHLDVFDLDAIAAIGKRHGLLTI
HHHHCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCEECHHHHHHHHHCCCEEEE
VDNTFMTPLLQRPLDHGIDMVIHSATKYLNGHGDVIAGMVAGSKEQIDKVKYQIIKDIGA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
VMSPHDAWLILRGMKTLDVRVQRHCDNAEKIADFLEAHPRVGRVYYPALKSHQGHRFLGT
HCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECHHHCCCCCHHHHH
QMRRAGGVIAFELKSDIEGSINFVDSLKVFTIAVSLGDAESLIQHPASMTHSPYTPEARL
HHHHHCCEEEEEECCCCCCCCHHHHCEEEEEEEEECCCHHHHHHCCHHCCCCCCCCCHHH
EAGITDTLLRISVGLEDVDDLIADLSQALAKI
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]