Click here to switch to the map view.

The map label for this gene is puuA [H]

Identifier: 119774311

GI number: 119774311

Start: 1433642

End: 1435009

Strand: Reverse

Name: puuA [H]

Synonym: Sama_1174

Alternate gene names: 119774311

Gene position: 1435009-1433642 (Counterclockwise)

Preceding gene: 119774316

Following gene: 119774309

Centisome position: 33.32

GC content: 57.75

Gene sequence:

>1368_bases
ATGCCCTTTGCCAATCCACAGGAAGCGATAGACTTTCTTGATAAACATCCCCAGGTTCAGCACGTCGAAGTCTTCATCAT
CGACCCCAATGGCATTCCCCGGGGCAAACTCCTGCACCGTCAGGAAGTGCTGTCTATGTATCGTCACGGCCGTCCTCTGC
CCAGCACTATTTTGGGACTGACGGTTCAGGGGGACGATGTAGAAGATACCGGTCTGGTTTGGGAAGTCGGTGACGCCGAC
TGCCTGGCGTTTCCCATCGAAGGCGGTTTGCTGATGCAGCCATGGCGTGCTCAGCCCACCGCTCAGGTGCATCTCACCAT
GCACCCCGAGGCGGGTATGCCCGCTGCTGTGGCCGATCCCCGCCTGGTGCTTTCCAAAGTAATAGATTCCCTGCAAGCCG
ATGGTTTTTATCCGGTAATGGCAGCAGAGTTGGAGTTTTTCCTGCTGGATCAGCGTTTCGATGCCAATGGCAGACCCCAG
CCAGCAATGCAAAGCGATGGCCACAGACCCCACCAAACTCAGGTGTATGGCATTTTGGAGTTGGAGCGACTGCAGCCCTT
CCTGGACGATCTTTACCACGCCTGTGAGGTGCAGGGCATTCCTGCGCGCACCGCCATTTCCGAGTATGCGCCCGGCCAGG
TAGAAATCACCCTCGAGCACAGATTCGACGCGCTTCAGGCCATGGATGAAGCGGTTCGCTACAAGCGACTGGTCAAGGGG
GTGGCCGCAAAACATGGTATGCAGGCCTGCTTCATGGCTAAACCCTTTGGTGAGTTGGCAGGCAGCGGCATGCATATGCA
CGTCAGCCTGGCCGACGCCGATGGCAACAACCGGTTTGCCAGTGACAACCCCGAAGGTACACCAGAGTTGACCCAATCCA
TCGCCGGCATGATGGCGACCCTGGAAGATGCCCAGTTGCTGTTTTGCCCCAATGCCAACTCCTTCCGCCGCTTTCAAAGC
GCAAGCTACGCCCCAATCGCCAAGACCTGGGGCGTGAACAACCGAACCGTTTCATTCAGGGTACCCGGTGGCCCGGCGCC
CAGTCGCCATGTGGAACATCGCATCTGTGGCGCAGACGCCAATCCTTATCTGGCGGCAGCGGCGATATTGGCGGCATGCC
ATCATGGTATCCGCAATCAGTTGTCACCCGGTCAGGCGATTGTCGGCAACGGCTACGAAGGCAAACATCAAACCCTGCCC
ACCGACTGGCTGTCGGCACTGACCAACTTCGAGCGCTCCGAGTGGATGAAGTCAGTATTGGGTGAGGATTTCCACCGTAT
TTACGGCAGAATTAAGCGCGCCGAGTACCAGGAGTTTATGGCCGAGGTTGGCCAGCAGGACTGGCACTGGTACTTAACCC
ACGCCTGA

Upstream 100 bases:

>100_bases
AAATACTGTCAAATAAATAAAACAACATGTTCAATATCAGTCAAAGCTTGTCGGAATTCTGTGGTATCCCCAAGCCGCGA
TTGCTCAACTCGAGGCCGCT

Downstream 100 bases:

>100_bases
TAATGACTACCTAAAAAGCAAAAAGCGCCCGAGGGCGCTTTTTAGTTTCAGCACTGCTAAAGGCTCAGTCCCACACCAGC
TCACTGAAGTGTTTGCGGCA

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MPFANPQEAIDFLDKHPQVQHVEVFIIDPNGIPRGKLLHRQEVLSMYRHGRPLPSTILGLTVQGDDVEDTGLVWEVGDAD
CLAFPIEGGLLMQPWRAQPTAQVHLTMHPEAGMPAAVADPRLVLSKVIDSLQADGFYPVMAAELEFFLLDQRFDANGRPQ
PAMQSDGHRPHQTQVYGILELERLQPFLDDLYHACEVQGIPARTAISEYAPGQVEITLEHRFDALQAMDEAVRYKRLVKG
VAAKHGMQACFMAKPFGELAGSGMHMHVSLADADGNNRFASDNPEGTPELTQSIAGMMATLEDAQLLFCPNANSFRRFQS
ASYAPIAKTWGVNNRTVSFRVPGGPAPSRHVEHRICGADANPYLAAAAILAACHHGIRNQLSPGQAIVGNGYEGKHQTLP
TDWLSALTNFERSEWMKSVLGEDFHRIYGRIKRAEYQEFMAEVGQQDWHWYLTHA

Sequences:

>Translated_455_residues
MPFANPQEAIDFLDKHPQVQHVEVFIIDPNGIPRGKLLHRQEVLSMYRHGRPLPSTILGLTVQGDDVEDTGLVWEVGDAD
CLAFPIEGGLLMQPWRAQPTAQVHLTMHPEAGMPAAVADPRLVLSKVIDSLQADGFYPVMAAELEFFLLDQRFDANGRPQ
PAMQSDGHRPHQTQVYGILELERLQPFLDDLYHACEVQGIPARTAISEYAPGQVEITLEHRFDALQAMDEAVRYKRLVKG
VAAKHGMQACFMAKPFGELAGSGMHMHVSLADADGNNRFASDNPEGTPELTQSIAGMMATLEDAQLLFCPNANSFRRFQS
ASYAPIAKTWGVNNRTVSFRVPGGPAPSRHVEHRICGADANPYLAAAAILAACHHGIRNQLSPGQAIVGNGYEGKHQTLP
TDWLSALTNFERSEWMKSVLGEDFHRIYGRIKRAEYQEFMAEVGQQDWHWYLTHA
>Mature_454_residues
PFANPQEAIDFLDKHPQVQHVEVFIIDPNGIPRGKLLHRQEVLSMYRHGRPLPSTILGLTVQGDDVEDTGLVWEVGDADC
LAFPIEGGLLMQPWRAQPTAQVHLTMHPEAGMPAAVADPRLVLSKVIDSLQADGFYPVMAAELEFFLLDQRFDANGRPQP
AMQSDGHRPHQTQVYGILELERLQPFLDDLYHACEVQGIPARTAISEYAPGQVEITLEHRFDALQAMDEAVRYKRLVKGV
AAKHGMQACFMAKPFGELAGSGMHMHVSLADADGNNRFASDNPEGTPELTQSIAGMMATLEDAQLLFCPNANSFRRFQSA
SYAPIAKTWGVNNRTVSFRVPGGPAPSRHVEHRICGADANPYLAAAAILAACHHGIRNQLSPGQAIVGNGYEGKHQTLPT
DWLSALTNFERSEWMKSVLGEDFHRIYGRIKRAEYQEFMAEVGQQDWHWYLTHA

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=434, Percent_Identity=24.4239631336406, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI87081870, Length=450, Percent_Identity=38, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1790301, Length=345, Percent_Identity=28.695652173913, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50501; Mature: 50370

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFANPQEAIDFLDKHPQVQHVEVFIIDPNGIPRGKLLHRQEVLSMYRHGRPLPSTILGL
CCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEE
TVQGDDVEDTGLVWEVGDADCLAFPIEGGLLMQPWRAQPTAQVHLTMHPEAGMPAAVADP
EEECCCCCCCCCEEEECCCCEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCH
RLVLSKVIDSLQADGFYPVMAAELEFFLLDQRFDANGRPQPAMQSDGHRPHQTQVYGILE
HHHHHHHHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHEE
LERLQPFLDDLYHACEVQGIPARTAISEYAPGQVEITLEHRFDALQAMDEAVRYKRLVKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
VAAKHGMQACFMAKPFGELAGSGMHMHVSLADADGNNRFASDNPEGTPELTQSIAGMMAT
HHHHHCCHHHHHCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEDAQLLFCPNANSFRRFQSASYAPIAKTWGVNNRTVSFRVPGGPAPSRHVEHRICGADA
HCCCEEEECCCCCHHHHHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCC
NPYLAAAAILAACHHGIRNQLSPGQAIVGNGYEGKHQTLPTDWLSALTNFERSEWMKSVL
CHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHH
GEDFHRIYGRIKRAEYQEFMAEVGQQDWHWYLTHA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
PFANPQEAIDFLDKHPQVQHVEVFIIDPNGIPRGKLLHRQEVLSMYRHGRPLPSTILGL
CCCCHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHEEE
TVQGDDVEDTGLVWEVGDADCLAFPIEGGLLMQPWRAQPTAQVHLTMHPEAGMPAAVADP
EEECCCCCCCCCEEEECCCCEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCCCCCCCH
RLVLSKVIDSLQADGFYPVMAAELEFFLLDQRFDANGRPQPAMQSDGHRPHQTQVYGILE
HHHHHHHHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEHEE
LERLQPFLDDLYHACEVQGIPARTAISEYAPGQVEITLEHRFDALQAMDEAVRYKRLVKG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
VAAKHGMQACFMAKPFGELAGSGMHMHVSLADADGNNRFASDNPEGTPELTQSIAGMMAT
HHHHHCCHHHHHCCCHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHH
LEDAQLLFCPNANSFRRFQSASYAPIAKTWGVNNRTVSFRVPGGPAPSRHVEHRICGADA
HCCCEEEECCCCCHHHHHHCCCCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHCCCCC
NPYLAAAAILAACHHGIRNQLSPGQAIVGNGYEGKHQTLPTDWLSALTNFERSEWMKSVL
CHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHH
GEDFHRIYGRIKRAEYQEFMAEVGQQDWHWYLTHA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]