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The map label for this gene is phoB [H]

Identifier: 119774211

GI number: 119774211

Start: 1300792

End: 1301481

Strand: Direct

Name: phoB [H]

Synonym: Sama_1074

Alternate gene names: 119774211

Gene position: 1300792-1301481 (Clockwise)

Preceding gene: 119774210

Following gene: 119774212

Centisome position: 30.21

GC content: 55.8

Gene sequence:

>690_bases
ATGACTGCAAGGATTTTGATTGTTGAAGATGAGTTGGCCATCCGCGAGATGCTGACATTCGTGATGGAACAACACGGTTT
CACGACCTCGGTTGCCGAAGATTTTGACTCGGCTCTTGGGCAGCTTCAGGAACCCTACCCAGATCTCATCCTGCTGGACT
GGATGTTTCCGGGCGGCAGTGGCATTCAACTTGCCAAACGCCTCAAGCAAGACGAGTTTACCCGCCAGATCCCGGTCATC
ATGCTCACCGCCCGTGGTGAGGAAGAAGACAAGGTCAAGGGCCTGGAAGTGGGGGCCGACGACTATATTACCAAGCCTTT
CTCCCCCAAGGAGCTGGTGGCCCGTATCAAGGCTGTACTGCGCCGCGCAGCGCCAACCCGCCTTGAAGAAACAATCGATG
TGCAGGGGTTGACCCTGGATCCGGTAAGCCACCGAGTCACAGCCGGTGATACAGTGCTGGATATGGGACCGACCGAGTTT
CGTCTGCTGCATTTCTTTATGACTCACCCCGAGCGTGTTTACAGCCGTGAACAGCTGCTGGATAACGTCTGGGGCACCAA
TGTGTACGTAGAAGACAGAACCGTAGACGTGCACATTCGTCGTTTGCGCAAGGCCGTTGAGCCTTCGGGGCATGACCGCC
TCATTCAAACCGTCCGCGGCGCAGGATACCGTTTTTCCACTCGGGTTTAG

Upstream 100 bases:

>100_bases
TTCTGCTGAAATGTGTCATCATTTACCCCGGCGATCATAAATTTGTCACAAAACTGACCAATAATGGCCATCGTGGACAT
TCACTGATAGAAAACCGCTT

Downstream 100 bases:

>100_bases
TTCGGGATAGGCACAGTTAGCCGGATAGGTTATCTTGTGCCCCCAGACACAACCCTCGGCTGGATATAGCTATGTTTGAC
TCTTATTCAGGGTATCGCCT

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTARILIVEDELAIREMLTFVMEQHGFTTSVAEDFDSALGQLQEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPVI
MLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRAAPTRLEETIDVQGLTLDPVSHRVTAGDTVLDMGPTEF
RLLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEPSGHDRLIQTVRGAGYRFSTRV

Sequences:

>Translated_229_residues
MTARILIVEDELAIREMLTFVMEQHGFTTSVAEDFDSALGQLQEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPVI
MLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRAAPTRLEETIDVQGLTLDPVSHRVTAGDTVLDMGPTEF
RLLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEPSGHDRLIQTVRGAGYRFSTRV
>Mature_228_residues
TARILIVEDELAIREMLTFVMEQHGFTTSVAEDFDSALGQLQEPYPDLILLDWMFPGGSGIQLAKRLKQDEFTRQIPVIM
LTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVLRRAAPTRLEETIDVQGLTLDPVSHRVTAGDTVLDMGPTEFR
LLHFFMTHPERVYSREQLLDNVWGTNVYVEDRTVDVHIRRLRKAVEPSGHDRLIQTVRGAGYRFSTRV

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=75.8771929824561, Blast_Score=369, Evalue=1e-104,
Organism=Escherichia coli, GI1788394, Length=226, Percent_Identity=40.2654867256637, Blast_Score=164, Evalue=3e-42,
Organism=Escherichia coli, GI1789809, Length=232, Percent_Identity=37.9310344827586, Blast_Score=147, Evalue=6e-37,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=37.7272727272727, Blast_Score=146, Evalue=9e-37,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=37.219730941704, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790860, Length=221, Percent_Identity=38.0090497737557, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1786911, Length=223, Percent_Identity=36.7713004484305, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI2367329, Length=229, Percent_Identity=41.0480349344978, Blast_Score=133, Evalue=8e-33,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=37.1794871794872, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787375, Length=225, Percent_Identity=31.1111111111111, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=35.9090909090909, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=33.4745762711864, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI145693140, Length=234, Percent_Identity=31.6239316239316, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=42.1052631578947, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=27.5, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=136, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26107; Mature: 25976

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARILIVEDELAIREMLTFVMEQHGFTTSVAEDFDSALGQLQEPYPDLILLDWMFPGGS
CCEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GIQLAKRLKQDEFTRQIPVIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRAAPTRLEETIDVQGLTLDPVSHRVTAGDTVLDMGPTEFRLLHFFMTHPERVYSREQLL
HHCCCHHHHHHHCCCCEEECCCCCEECCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVEPSGHDRLIQTVRGAGYRFSTRV
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TARILIVEDELAIREMLTFVMEQHGFTTSVAEDFDSALGQLQEPYPDLILLDWMFPGGS
CEEEEEEECHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
GIQLAKRLKQDEFTRQIPVIMLTARGEEEDKVKGLEVGADDYITKPFSPKELVARIKAVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCCCCCHHHHHHHHHHHH
RRAAPTRLEETIDVQGLTLDPVSHRVTAGDTVLDMGPTEFRLLHFFMTHPERVYSREQLL
HHCCCHHHHHHHCCCCEEECCCCCEECCCCEEEECCCHHHHHHHHHHHCHHHHHHHHHHH
DNVWGTNVYVEDRTVDVHIRRLRKAVEPSGHDRLIQTVRGAGYRFSTRV
HHHCCCCEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]