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The map label for this gene is radA [H]

Identifier: 119774118

GI number: 119774118

Start: 1186543

End: 1187907

Strand: Direct

Name: radA [H]

Synonym: Sama_0981

Alternate gene names: 119774118

Gene position: 1186543-1187907 (Clockwise)

Preceding gene: 119774116

Following gene: 119774120

Centisome position: 27.55

GC content: 57.29

Gene sequence:

>1365_bases
ATGGCAAAAAATAAAACCGCGTACGTATGCAATGAGTGTGGTCAGGACTTTCCCCGCTGGCAAGGGCAGTGCAGCGCTTG
CCTGGAGTGGAACTCCATCACCGAAGTGAGACTGGGCGCCGCCACTGTGGCACGCAGCGGACGTTTCAGCGGATATGCTG
GTGCCGGGGTATCTGAGGTCAAAACTCTCGACCAAATTGATTTGAATGAGCTGCCCCGAATCCCAAGCTCCTTTACCGAG
TTTGACCGGGTTCTGGGCGGAGGCATAGTGCCCGGCAGCGCCATCCTGATTGGTGGTCATCCGGGAGCGGGTAAAAGTAC
GCTGTTATTGCAGACCCTGTGTCAGTTAGCGGAGGTCATGCCGGCTCTCTATGTGACCGGCGAAGAGTCGCTGCAGCAGG
TGGCCATGCGGGCTCATCGACTGGGGCTGCCTACTTCCAAATTAAAGATGCTCTCTGAAACCAGTGTCGAAACCCTCTGT
GACATTGCCCTGAAAGAACAGCCCAAGCTGATAGTGGTGGACTCCATTCAGGTGATGCACATGAGTGATGTGCAGTCCTC
CCCCGGCAGTGTGGCACAGGTGCGTGAATCAGCGTCATTCCTTACCCGGTTTGCCAAGCAAAACGGTATCGCCGTGATCA
TGGTCGGTCACGTCACCAAAGACGGCAGTCTCGCTGGCCCCAAGGTGCTTGAGCACTGTATCGACTGCTCTGTGATGTTT
GAAGGCGACTCCGACAGCCGTTATCGCACCCTCCGTTCCCATAAAAACCGTTTTGGTGCCATCAATGAGCTTGGCGTCTT
TGCCATGACTGAGCGGGGCCTCAAAGAGGTGGCCAACCCGTCGGCAATTTTCCTCTCCCGTGGAGAAGAGGCTTCCAGTG
GCTCCCTGGTGATGGTGGTCTGGGAAGGTACCCGGCCGCTGCTGGTGGAGCTGCAGGTGCTGGTGGATAACTCGGCGCTG
TCCAATCCGCGGCGGGTGGCGGTGGGGATGGATGCCAACCGTCTGGCCATGTTACTGGCGGTGATGCATCGCCATGGCGG
CCTGCAAATGTCGGATCAGGACGTGTTTGTGAACGTAGTGGGTGGGGTAAAGGTCACCGAAACCAGCGCCGACCTGACCT
TATTGCTGGCCATGGTGTCCAGTTTCCGTGGCGAAGTCTTGCCAAGGGATCTGGTGGCCTTTGGTGAGGTGGGACTTTCG
GGCGAAATTCGCCCCGTGCCCAACGGTCAGGAGCGATTGGTGGAAGCGGCCAAGCACGGCTTTAAACGGGCGATTGTGCC
AAGAGCCAATATGCCGAAAAAGCCACCCGAAGGCATGGAAGTGATTGGCGTATCAAAGCTAGCTGAAGCCTTGGCAGCCC
TTTAA

Upstream 100 bases:

>100_bases
ATTTGTCTTTTTGCCGTGCCATTGGAGAGAGGAATTGCTAGTCTATTATCGGCAGCTTTTCTGGGTTCTATACTCAATTT
TATACAGAATTTAGGCAGTT

Downstream 100 bases:

>100_bases
ACAAAAGTGTCAATCACATGAAAAAGGGGACATCAGTCCCCTTTTTCATGGCTCTCGATAAAGTGTTCCAGCTCTCGATT
GAGCAGGGATGCATCCCCCA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACLEWNSITEVRLGAATVARSGRFSGYAGAGVSEVKTLDQIDLNELPRIPSSFTE
FDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCQLAEVMPALYVTGEESLQQVAMRAHRLGLPTSKLKMLSETSVETLC
DIALKEQPKLIVVDSIQVMHMSDVQSSPGSVAQVRESASFLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEASSGSLVMVVWEGTRPLLVELQVLVDNSAL
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEVLPRDLVAFGEVGLS
GEIRPVPNGQERLVEAAKHGFKRAIVPRANMPKKPPEGMEVIGVSKLAEALAAL

Sequences:

>Translated_454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACLEWNSITEVRLGAATVARSGRFSGYAGAGVSEVKTLDQIDLNELPRIPSSFTE
FDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCQLAEVMPALYVTGEESLQQVAMRAHRLGLPTSKLKMLSETSVETLC
DIALKEQPKLIVVDSIQVMHMSDVQSSPGSVAQVRESASFLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEASSGSLVMVVWEGTRPLLVELQVLVDNSAL
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEVLPRDLVAFGEVGLS
GEIRPVPNGQERLVEAAKHGFKRAIVPRANMPKKPPEGMEVIGVSKLAEALAAL
>Mature_453_residues
AKNKTAYVCNECGQDFPRWQGQCSACLEWNSITEVRLGAATVARSGRFSGYAGAGVSEVKTLDQIDLNELPRIPSSFTEF
DRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCQLAEVMPALYVTGEESLQQVAMRAHRLGLPTSKLKMLSETSVETLCD
IALKEQPKLIVVDSIQVMHMSDVQSSPGSVAQVRESASFLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMFE
GDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEASSGSLVMVVWEGTRPLLVELQVLVDNSALS
NPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGEVLPRDLVAFGEVGLSG
EIRPVPNGQERLVEAAKHGFKRAIVPRANMPKKPPEGMEVIGVSKLAEALAAL

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=76.8052516411379, Blast_Score=732, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48677; Mature: 48546

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTAYVCNECGQDFPRWQGQCSACLEWNSITEVRLGAATVARSGRFSGYAGAGVSEV
CCCCCCCHHHHHHCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
KTLDQIDLNELPRIPSSFTEFDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCQLAEVM
HHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
PALYVTGEESLQQVAMRAHRLGLPTSKLKMLSETSVETLCDIALKEQPKLIVVDSIQVMH
HHEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEE
MSDVQSSPGSVAQVRESASFLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEASSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSALSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGEVLPRDLVAFGEVGLSGEIRPVPNGQERLVEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPRANMPKKPPEGMEVIGVSKLAEALAAL
HHEECCCCCCCCCCCCCCCCEECHHHHHHHHHCC
>Mature Secondary Structure 
AKNKTAYVCNECGQDFPRWQGQCSACLEWNSITEVRLGAATVARSGRFSGYAGAGVSEV
CCCCCCHHHHHHCCCCCCCCCCHHHEEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHH
KTLDQIDLNELPRIPSSFTEFDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCQLAEVM
HHHHCCCHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHH
PALYVTGEESLQQVAMRAHRLGLPTSKLKMLSETSVETLCDIALKEQPKLIVVDSIQVMH
HHEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEE
MSDVQSSPGSVAQVRESASFLTRFAKQNGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEASSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSALSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEECCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGEVLPRDLVAFGEVGLSGEIRPVPNGQERLVEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPRANMPKKPPEGMEVIGVSKLAEALAAL
HHEECCCCCCCCCCCCCCCCEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]