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The map label for this gene is thyA

Identifier: 119774004

GI number: 119774004

Start: 1058241

End: 1059035

Strand: Direct

Name: thyA

Synonym: Sama_0867

Alternate gene names: 119774004

Gene position: 1058241-1059035 (Clockwise)

Preceding gene: 119774003

Following gene: 119774005

Centisome position: 24.58

GC content: 52.2

Gene sequence:

>795_bases
ATGAAGCAGTATCTCGACTTAATGAAGCACATCCTCGATAAGGGGACAGATAAAAGTGACAGAACCGGTACAGGCACCCG
TTCGGTTTTCGGTTATCAGATGCGCTTTGACCTCAATGAGGGGTTTCCGCTGGTTACCACCAAGAAGTGCCACCTGCGCT
CCATCATTCATGAACTGCTGTGGTTCCTGAAAGGTGAAACCAATGTGGATTATCTGCACGAAAACAAGGTGTCGATTTGG
GACGAGTGGGCCGATGAAAAGGGCAATCTGGGACCTGTCTATGGCGCCCAATGGCGCAGCTGGCCAACCCCTGATGGCCG
CCATATTGATCAGATTTCTCAAGTTATTGAGCAGATTAAAGCCACGCCGGACTCTCGCCGGTTAATCGTATCAGCCTGGA
ACGTGGGAGAGCTGGATAAAATGGCGCTGGCACCCTGCCATGCGTTTTTCCAATTTTACGTGGCCGATGGCAAACTGAGC
TGCCAGCTCTATCAGCGTTCCTGTGATGTGTTCCTGGGCCTGCCGTTTAATATTGCCAGTTATGCGCTGCTGACCATGAT
GGTGGCGCAGCAGTGTAATCTTGGCTTGGGCGATTTCGTTTGGACCGGTGGGGATACCCACCTCTACAGCAACCACATGG
AGCAGACTCAATTGCAGCTCTCACGGGAGCCTCGTCCTCTGCCCACCATGAAGATCCTCCGCCATCCAGAGTCGATTTTT
GATTACCGTTTCGAAGACTTTGAACTCAGTGGTTACGACCCCCATCCCCACATCAAGGCGCCCGTTGCTATCTAA

Upstream 100 bases:

>100_bases
TGGGCTTTTACCTCGGTTTTATTACCATGGGCCAAATTCTTTCTCTGCCGATGATCCTCTTCGGTCTTTATTTGATTTTG
CGTAAACAGGAAACCCGCTG

Downstream 100 bases:

>100_bases
AATGGGTGAACTTTCGCCTGATACCTCGGAAATTTCGAGGTTTCAGGCAGAAACTTACGATTATTTTTGATAGTCTTAAT
TCGCTACACTCAATGCATTC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKQYLDLMKHILDKGTDKSDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKCHLRSIIHELLWFLKGETNVDYLHENKVSIW
DEWADEKGNLGPVYGAQWRSWPTPDGRHIDQISQVIEQIKATPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCNLGLGDFVWTGGDTHLYSNHMEQTQLQLSREPRPLPTMKILRHPESIF
DYRFEDFELSGYDPHPHIKAPVAI

Sequences:

>Translated_264_residues
MKQYLDLMKHILDKGTDKSDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKCHLRSIIHELLWFLKGETNVDYLHENKVSIW
DEWADEKGNLGPVYGAQWRSWPTPDGRHIDQISQVIEQIKATPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCNLGLGDFVWTGGDTHLYSNHMEQTQLQLSREPRPLPTMKILRHPESIF
DYRFEDFELSGYDPHPHIKAPVAI
>Mature_264_residues
MKQYLDLMKHILDKGTDKSDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKCHLRSIIHELLWFLKGETNVDYLHENKVSIW
DEWADEKGNLGPVYGAQWRSWPTPDGRHIDQISQVIEQIKATPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
CQLYQRSCDVFLGLPFNIASYALLTMMVAQQCNLGLGDFVWTGGDTHLYSNHMEQTQLQLSREPRPLPTMKILRHPESIF
DYRFEDFELSGYDPHPHIKAPVAI

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=50.354609929078, Blast_Score=282, Evalue=2e-76,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=81.8181818181818, Blast_Score=472, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=45.2296819787986, Blast_Score=249, Evalue=7e-67,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=39.3835616438356, Blast_Score=201, Evalue=1e-52,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=50.530035335689, Blast_Score=276, Evalue=7e-75,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_SHEAM (A1S3W8)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926744.1
- ProteinModelPortal:   A1S3W8
- SMR:   A1S3W8
- STRING:   A1S3W8
- GeneID:   4603119
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0867
- NMPDR:   fig|326297.7.peg.817
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   A1S3W8
- ProtClustDB:   PRK01827
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30411; Mature: 30411

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQYLDLMKHILDKGTDKSDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGETNVDYLHENKVSIWDEWADEKGNLGPVYGAQWRSWPTPDGRHIDQISQVIEQIK
HHHCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ATPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCNLGLGDFVWTGGDTHLYSNHMEQTQLQLSREPRPLPTMKILRHPESIF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHH
DYRFEDFELSGYDPHPHIKAPVAI
HCCCCCEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKQYLDLMKHILDKGTDKSDRTGTGTRSVFGYQMRFDLNEGFPLVTTKKCHLRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCCHHEEEEEEEEECCCCCCEEECCHHHHHHHHHHHH
WFLKGETNVDYLHENKVSIWDEWADEKGNLGPVYGAQWRSWPTPDGRHIDQISQVIEQIK
HHHCCCCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
ATPDSRRLIVSAWNVGELDKMALAPCHAFFQFYVADGKLSCQLYQRSCDVFLGLPFNIAS
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCEEEECCHHHHH
YALLTMMVAQQCNLGLGDFVWTGGDTHLYSNHMEQTQLQLSREPRPLPTMKILRHPESIF
HHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHH
DYRFEDFELSGYDPHPHIKAPVAI
HCCCCCEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA