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The map label for this gene is rlmA [H]

Identifier: 119773969

GI number: 119773969

Start: 1015265

End: 1016080

Strand: Direct

Name: rlmA [H]

Synonym: Sama_0832

Alternate gene names: 119773969

Gene position: 1015265-1016080 (Clockwise)

Preceding gene: 119773968

Following gene: 119773972

Centisome position: 23.58

GC content: 49.75

Gene sequence:

>816_bases
ATGGAGTTTCAGTGTCCACTTTGTCGCCAACGGCTGCACTTTCACGAAGAGTCCCGTGGATGGCATTGCGATGGCAAACA
TCACTTCGACAAGAAAGAAATCGGCTATTGGGATTTCTACAAGGGCAACAAACCCCGTAATGCCGAAAGCCGTCAGGAGC
TGCGTGCCAGACAGTTTCTGGTGACCTCTGGCCTGTTTCAGCCAGCAGTAGAGGCCATGGTGAAGGTGCTGCAAACCCAG
GACTGCGGAAGCGAGCGCAACTGCGTTAACCTGGGGCCAGGACATGCCTGGATTGGAAAGTTATTGGCGCAAGCGCTGTC
TGAACATGAGTTGCCACTTAAGGTATTCAGTCCATTGGAGTCAGAAAACGAAGCTTTTGCTGCAGCCAAATCAGGAATGG
AGGCCGTTTGTCTGACACCGCTCAAATCGCTGCCCTTTGCCGATGCATCAGCCGATATGGTGTGGCTTCTGGATATTCCA
GCCAAAGGAAAGGAATGGCAGCGAATACTGAAACCGGGCGGATTGTTATTGATGTTGGCAGCCGGACCAAGACACCTTTG
GCAAATTAAAGAATTTGTCTATGAAGGATTAACTGAAAAGCCATTTGTGTTGGAGCTTTCCGGCCAATTTGACTTGATAA
GTCAAACCCCGGTTTCATGGACTCATGCCCTCAGTGTGGCTGATGCCAAGGTATTAATGGGTATGGCTCCCTGGGCCTGG
CGGGTAAAAGAAGCCAGTTTAAAGGCGATGGAAAAGGGAGATTTTTCCGGATTGGAAATGGATTATCGGATCATTCTTGC
CAAAAAGCGGGTATAA

Upstream 100 bases:

>100_bases
TGGGTGTATTCCTCTGCGGCTTTATTTATTTGTAATAGTTGCGTAATTCAGATTGACCAAAGGGGCGGCATTGGCCGCCT
CTTTGATTTAAGGAGTTTTC

Downstream 100 bases:

>100_bases
CTGGCAAGAAAGCGAAAATATCGCGAAAAACAGGTAAAAAAATACCGGCAACGCCCGAAAGCATTGCCGGTACCAACACA
GCATTACTTCTTGTAAGAAG

Product: ribosomal RNA large subunit methyltransferase A

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MEFQCPLCRQRLHFHEESRGWHCDGKHHFDKKEIGYWDFYKGNKPRNAESRQELRARQFLVTSGLFQPAVEAMVKVLQTQ
DCGSERNCVNLGPGHAWIGKLLAQALSEHELPLKVFSPLESENEAFAAAKSGMEAVCLTPLKSLPFADASADMVWLLDIP
AKGKEWQRILKPGGLLLMLAAGPRHLWQIKEFVYEGLTEKPFVLELSGQFDLISQTPVSWTHALSVADAKVLMGMAPWAW
RVKEASLKAMEKGDFSGLEMDYRIILAKKRV

Sequences:

>Translated_271_residues
MEFQCPLCRQRLHFHEESRGWHCDGKHHFDKKEIGYWDFYKGNKPRNAESRQELRARQFLVTSGLFQPAVEAMVKVLQTQ
DCGSERNCVNLGPGHAWIGKLLAQALSEHELPLKVFSPLESENEAFAAAKSGMEAVCLTPLKSLPFADASADMVWLLDIP
AKGKEWQRILKPGGLLLMLAAGPRHLWQIKEFVYEGLTEKPFVLELSGQFDLISQTPVSWTHALSVADAKVLMGMAPWAW
RVKEASLKAMEKGDFSGLEMDYRIILAKKRV
>Mature_271_residues
MEFQCPLCRQRLHFHEESRGWHCDGKHHFDKKEIGYWDFYKGNKPRNAESRQELRARQFLVTSGLFQPAVEAMVKVLQTQ
DCGSERNCVNLGPGHAWIGKLLAQALSEHELPLKVFSPLESENEAFAAAKSGMEAVCLTPLKSLPFADASADMVWLLDIP
AKGKEWQRILKPGGLLLMLAAGPRHLWQIKEFVYEGLTEKPFVLELSGQFDLISQTPVSWTHALSVADAKVLMGMAPWAW
RVKEASLKAMEKGDFSGLEMDYRIILAKKRV

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=271, Percent_Identity=28.4132841328413, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 30649; Mature: 30649

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFQCPLCRQRLHFHEESRGWHCDGKHHFDKKEIGYWDFYKGNKPRNAESRQELRARQFL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VTSGLFQPAVEAMVKVLQTQDCGSERNCVNLGPGHAWIGKLLAQALSEHELPLKVFSPLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCC
SENEAFAAAKSGMEAVCLTPLKSLPFADASADMVWLLDIPAKGKEWQRILKPGGLLLMLA
CCCHHHHHHHCCCCEEEECCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEE
AGPRHLWQIKEFVYEGLTEKPFVLELSGQFDLISQTPVSWTHALSVADAKVLMGMAPWAW
CCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHH
RVKEASLKAMEKGDFSGLEMDYRIILAKKRV
HHHHHHHHHHHCCCCCCCCEEEEEEEEECCC
>Mature Secondary Structure
MEFQCPLCRQRLHFHEESRGWHCDGKHHFDKKEIGYWDFYKGNKPRNAESRQELRARQFL
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHH
VTSGLFQPAVEAMVKVLQTQDCGSERNCVNLGPGHAWIGKLLAQALSEHELPLKVFSPLE
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCHHHHCCCC
SENEAFAAAKSGMEAVCLTPLKSLPFADASADMVWLLDIPAKGKEWQRILKPGGLLLMLA
CCCHHHHHHHCCCCEEEECCHHHCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEE
AGPRHLWQIKEFVYEGLTEKPFVLELSGQFDLISQTPVSWTHALSVADAKVLMGMAPWAW
CCCHHHHHHHHHHHCCCCCCCEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCHH
RVKEASLKAMEKGDFSGLEMDYRIILAKKRV
HHHHHHHHHHHCCCCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]