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The map label for this gene is aspA [H]

Identifier: 119773926

GI number: 119773926

Start: 971562

End: 972980

Strand: Direct

Name: aspA [H]

Synonym: Sama_0789

Alternate gene names: 119773926

Gene position: 971562-972980 (Clockwise)

Preceding gene: 119773919

Following gene: 119773930

Centisome position: 22.56

GC content: 59.06

Gene sequence:

>1419_bases
ATGGATACACGTATTGAACACGACCTGCTGGGGGATGCCCCGGTTCCTGCCCAAGCCTGGTATGGCATTCAAACCCAGAG
AGCGCTGGAAAACTTCAGCCTGAGCGGTACCCCAATCAATGCTTTCCCTGAGCTTATCAGGGCGCTGGCCAAGGTTAAGG
CCGCTGCCGCCCGCGCCAACCAGAGTCTCGGGCAGCTGTCTGACATCAAAGCCAACGCCATTGCCGCCGCCTGCGACGAT
ATTGTTCAGGGCGCACTGCACGACCAGTTTGTGGTGGATCTCATTCAGGGGGGCGCTGGCACCTCCACCAACATGAACGC
CAACGAAGTGATTGCCAACCTGGCGCTTGCCAAGCTTGGCCATGGCAAGGGAGAGTACCGCCATCTGCACCCCAACAATG
ATGTGAACTGCTCACAGTCCACCAACGATGCCTACCCGACGGCGGCGCGTCTGGCCATGGTGGAGGCGACAGCCCCACTC
AAGGTGGCTATCGAGGCCATTTGCTTAAGCCTTGAGGTCAAGGGCCGGGAGTTTAGCCATATCCTCAAGATGGGCCGCAC
CCAGCTGCAGGATGCGGTGCCCATGACCCTGGGGCAAGAGTTCGATGCTTTTGCCTCCAGCCTTAAATCGGACATAGGCC
GCATCGATGATGCCTGCAAGGAGCTTTGCGTGGTGAACCTTGGCGGCACCGCCATTGGCACCGGTATTAACACCCATCCG
GCCTATGGCGTGTTGGCAGTAAAGGCCCTTGCCGACATTACTGGACTGCCGGTGACTCAGGCTGACAATCTAATCGATGC
CACCACCGATATGGGCGCCTTTGTGACGCTGTCATCTGTGCTTAAGCGCCTGGCGGTGAAGCTGTCCAAGCTCAGTAATG
ACCTGCGGTTGCTCTCCAGCGGTCCACGCACCGGTCTTGGGGAAATTCGTTTGCCTGCGATGCAGCCGGGATCCTCCATC
ATGCCGGGCAAGGTGAATCCGGTTATCCCTGAGGCGGTGAATCAGGTGGCGTTTCAGGTGATTGGCACCGACATGACCAT
CACCATGGCCGCCGAGGCGGCGCAGCTGCAACTGAATGCCATGGAACCGGTGATTGTTTATAACCTTTTGAATAACTGCG
CCTTGCTGACCCGTGCCATTGCCATGCTCGACAGCCGCTGTATTCAGGGCATCGAAGCCAACGAAGCCAAATGCGAGCAG
CATGTGAGCAGCAGTATCGGCATAGTCACGGCGTTGGTGCCCCACATCGGCTACGAGAACGCCACCCGTATCGCGGGTGA
AGCGCTGCTGAGTGGCGCCGGTGTGGCCGAGCTTGTGCGCCGCGATGGGCTGCTTAGCGATGATGCCTTAAGCCAAATCT
TAAGTCCGGCCGCCATGGTGACGCCGGTGGAGCTGACTGGTGCCGCCAAGGCGCATTGA

Upstream 100 bases:

>100_bases
CTCCCTTAATGCAGTGCAGGCTGCTGTAATAGCGCGAGTTTATCGGGCTTGGTCATAGAAAAAGCCCAGCTCACACAGCG
TCCGGGAGCGCACCATAAAG

Downstream 100 bases:

>100_bases
TTCAGCGTTTTTTAAAATATAACGCGATAAAAAAGCAGCCACCCGGCTGCTTTTTTGTGTGTGCTTGAGTCTCACAAAGT
CAGAAACTTAACTTGTATGC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDTRIEHDLLGDAPVPAQAWYGIQTQRALENFSLSGTPINAFPELIRALAKVKAAAARANQSLGQLSDIKANAIAAACDD
IVQGALHDQFVVDLIQGGAGTSTNMNANEVIANLALAKLGHGKGEYRHLHPNNDVNCSQSTNDAYPTAARLAMVEATAPL
KVAIEAICLSLEVKGREFSHILKMGRTQLQDAVPMTLGQEFDAFASSLKSDIGRIDDACKELCVVNLGGTAIGTGINTHP
AYGVLAVKALADITGLPVTQADNLIDATTDMGAFVTLSSVLKRLAVKLSKLSNDLRLLSSGPRTGLGEIRLPAMQPGSSI
MPGKVNPVIPEAVNQVAFQVIGTDMTITMAAEAAQLQLNAMEPVIVYNLLNNCALLTRAIAMLDSRCIQGIEANEAKCEQ
HVSSSIGIVTALVPHIGYENATRIAGEALLSGAGVAELVRRDGLLSDDALSQILSPAAMVTPVELTGAAKAH

Sequences:

>Translated_472_residues
MDTRIEHDLLGDAPVPAQAWYGIQTQRALENFSLSGTPINAFPELIRALAKVKAAAARANQSLGQLSDIKANAIAAACDD
IVQGALHDQFVVDLIQGGAGTSTNMNANEVIANLALAKLGHGKGEYRHLHPNNDVNCSQSTNDAYPTAARLAMVEATAPL
KVAIEAICLSLEVKGREFSHILKMGRTQLQDAVPMTLGQEFDAFASSLKSDIGRIDDACKELCVVNLGGTAIGTGINTHP
AYGVLAVKALADITGLPVTQADNLIDATTDMGAFVTLSSVLKRLAVKLSKLSNDLRLLSSGPRTGLGEIRLPAMQPGSSI
MPGKVNPVIPEAVNQVAFQVIGTDMTITMAAEAAQLQLNAMEPVIVYNLLNNCALLTRAIAMLDSRCIQGIEANEAKCEQ
HVSSSIGIVTALVPHIGYENATRIAGEALLSGAGVAELVRRDGLLSDDALSQILSPAAMVTPVELTGAAKAH
>Mature_472_residues
MDTRIEHDLLGDAPVPAQAWYGIQTQRALENFSLSGTPINAFPELIRALAKVKAAAARANQSLGQLSDIKANAIAAACDD
IVQGALHDQFVVDLIQGGAGTSTNMNANEVIANLALAKLGHGKGEYRHLHPNNDVNCSQSTNDAYPTAARLAMVEATAPL
KVAIEAICLSLEVKGREFSHILKMGRTQLQDAVPMTLGQEFDAFASSLKSDIGRIDDACKELCVVNLGGTAIGTGINTHP
AYGVLAVKALADITGLPVTQADNLIDATTDMGAFVTLSSVLKRLAVKLSKLSNDLRLLSSGPRTGLGEIRLPAMQPGSSI
MPGKVNPVIPEAVNQVAFQVIGTDMTITMAAEAAQLQLNAMEPVIVYNLLNNCALLTRAIAMLDSRCIQGIEANEAKCEQ
HVSSSIGIVTALVPHIGYENATRIAGEALLSGAGVAELVRRDGLLSDDALSQILSPAAMVTPVELTGAAKAH

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=40, Blast_Score=335, Evalue=4e-92,
Organism=Escherichia coli, GI87082375, Length=462, Percent_Identity=54.1125541125541, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1787896, Length=462, Percent_Identity=41.3419913419913, Blast_Score=339, Evalue=2e-94,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=39.9132321041215, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=39.2638036809816, Blast_Score=246, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324993, Length=465, Percent_Identity=40.6451612903226, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=40, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=39.0928725701944, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=39.0928725701944, Blast_Score=331, Evalue=5e-91,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=37.6623376623377, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI24583245, Length=464, Percent_Identity=36.4224137931034, Blast_Score=306, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 49472; Mature: 49472

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTRIEHDLLGDAPVPAQAWYGIQTQRALENFSLSGTPINAFPELIRALAKVKAAAARAN
CCCCCCHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSLGQLSDIKANAIAAACDDIVQGALHDQFVVDLIQGGAGTSTNMNANEVIANLALAKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
HGKGEYRHLHPNNDVNCSQSTNDAYPTAARLAMVEATAPLKVAIEAICLSLEVKGREFSH
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCHHHH
ILKMGRTQLQDAVPMTLGQEFDAFASSLKSDIGRIDDACKELCVVNLGGTAIGTGINTHP
HHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCCCCC
AYGVLAVKALADITGLPVTQADNLIDATTDMGAFVTLSSVLKRLAVKLSKLSNDLRLLSS
CHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRTGLGEIRLPAMQPGSSIMPGKVNPVIPEAVNQVAFQVIGTDMTITMAAEAAQLQLNA
CCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCC
MEPVIVYNLLNNCALLTRAIAMLDSRCIQGIEANEAKCEQHVSSSIGIVTALVPHIGYEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ATRIAGEALLSGAGVAELVRRDGLLSDDALSQILSPAAMVTPVELTGAAKAH
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCHHHCCCEEECCCCCCC
>Mature Secondary Structure
MDTRIEHDLLGDAPVPAQAWYGIQTQRALENFSLSGTPINAFPELIRALAKVKAAAARAN
CCCCCCHHHCCCCCCCHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QSLGQLSDIKANAIAAACDDIVQGALHDQFVVDLIQGGAGTSTNMNANEVIANLALAKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
HGKGEYRHLHPNNDVNCSQSTNDAYPTAARLAMVEATAPLKVAIEAICLSLEVKGREFSH
CCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEECCCHHHH
ILKMGRTQLQDAVPMTLGQEFDAFASSLKSDIGRIDDACKELCVVNLGGTAIGTGINTHP
HHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEECCCCCCCC
AYGVLAVKALADITGLPVTQADNLIDATTDMGAFVTLSSVLKRLAVKLSKLSNDLRLLSS
CHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPRTGLGEIRLPAMQPGSSIMPGKVNPVIPEAVNQVAFQVIGTDMTITMAAEAAQLQLNA
CCCCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHCC
MEPVIVYNLLNNCALLTRAIAMLDSRCIQGIEANEAKCEQHVSSSIGIVTALVPHIGYEN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
ATRIAGEALLSGAGVAELVRRDGLLSDDALSQILSPAAMVTPVELTGAAKAH
HHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHCCHHHCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3096982 [H]