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The map label for this gene is fusA2 [H]

Identifier: 119773722

GI number: 119773722

Start: 729256

End: 731346

Strand: Direct

Name: fusA2 [H]

Synonym: Sama_0584

Alternate gene names: 119773722

Gene position: 729256-731346 (Clockwise)

Preceding gene: 119773721

Following gene: 119773723

Centisome position: 16.94

GC content: 52.13

Gene sequence:

>2091_bases
ATGACCGATTTATCAAAATACAGAAACATTGGTATTTTCGCTCACGTTGACGCGGGTAAAACCACGACCACCGAGCGTAT
CCTCAAGCTGACCGGTAAAATCCACAAACTGGGCGAAGTTCACGATGGCGCTTCTACCATGGACTTCATGGATCAGGAAG
CAGAGCGTGGTATTACCATTCAGTCTGCTGCCACCACCTGTTTCTGGAAAGGTCACCGCTTTAACGTAATCGACACCCCC
GGTCACGTTGACTTCACCGTTGAAGTTTATCGTTCTCTGAAGGTTCTGGACGGCGGTATCGGTGTATTCTGCGGTAGCGG
CGGTGTAGAACCACAATCAGAAACCAACTGGCGTTATGCCAACGAGTCTGAAGTAGCTCGTTTGATCTTCGTAAACAAGC
TGGACCGTATCGGTGCCGACTTCCTGCGCGTAGTAGGTCAGGTTAAGAAAGTACTGGCTGCCAACCCGCTGGTAATGACT
CTGCCTATCGGTCGTGAAGACGAATTCACAGGCGTTGTTGACGTACTGAACCGTCAGGCTTTCATTTGGGACGATTCAGG
TCTGCCAGAAAACTACACTGTGACCGACGTTCCTGCCGACATGGTTGACCTGGTTGAAGAATACCGTGAAATGCTGGTTG
AGACTGCCGTTGAGCAGGACGACGACCTGATGGAAGCTTACATGGAAGGTGAAGAGCCTTCTATCGAAGACCTGAAGCGT
TGTATCCGTAAGGGTACCATCAACCTGTCTTTCTTCCCTACCTTCTGCGGCTCTGCGTTCAAGAACAAAGGTATGCAGCT
GGTACTGGACGCAGTAGTTGACTACCTGCCTTCTCCAACTGAAGTTGAGCCACAGCCTCTGACTGATCCAGAGACTGGTG
AAGAAACCGGCAAAGTTGCTACCGTTTCTGTAGACGAGCCACTGCGCGCTCTGGCGTTCAAGATCATGGACGACCGTTTC
GGCGCCCTGACCTTCGTACGTATCTATTCTGGTAAGCTGAAGAAGGGTGACACCATCCTGAACTCTGCTACCGGTAAAAC
TGAACGCATCGGCCGTATGGTGGAAATGCACGCCAACGATCGTAACGAAATCGACTCTGCCCAGGCTGGTGACATCATCG
CCGTTGTAGGTATGAAGAACGTTCAGACCGGTCACACCCTGTGTGATCCTAAGCACGAGTGTACTCTCGAGCCAATGATC
TTCCCAACGCCTGTAATCTCTATCGCTGTTACTCCTAAGGACAAAGGCGCTTCTGAGAAGCTGGGTGTTGCTCTGGGTAA
GATGGTTGCTGAAGATCCATCATTCCAGGTTGAAACCGACCAGGAAACCGGTGACACCATCCTGAAAGGTATGGGTGAGC
TGCACCTGGACATCAAGGTAGACATCCTGAAGCGTACTTTCGGCGTTGAGCTGACTGTAGGTGCTCCACAGGTAGCCTAT
CGTGAAACCATCACTGCCGCCATCGAAGACAGCTACACTCACAAGAAGCAGTCTGGTGGTTCTGGTCAGTACGGTAAGAT
TGACTACCGCATCCGTCCTGGTGAGCCAGGTACCGGCTTCACCTTCAAGTCTGCCGTAGTGGGTGGTAACGTTCCTCGTG
AATTCTGGCCTGCCGTTGAGTCTGGCTTCGAAGAAATGATGAGCCAAGGTCCTCTGGCTGGCTTCCCCGTACTGGACGTT
GAAGTTGAGCTGTTTGACGGTGCTTACCACGCAGTTGACTCCTCTGCCATCGCGTTCGAAATCGCCGCTAAAGGTGCATT
CCGTCAGTCCATGCCTAAGGCCAAGCCACAGCTGCTCGAGCCTATCATGAAGGTTGACGTGTTCACTCCAGACGATCACG
TAGGTGACGTAATCGGTGACCTGAACCGTCGTCGCGGTATGATCAAGGATCAGGAAGCTGGTCTGACTGGCGTACGCGTT
AAGGCAGACGTACCGCTGGCCGAAATGTTCGGTTACATCGGTCACCTGCGTACCATGACCTCTGGTCGTGGTCAGTTCTC
CATGGAATTCTCTCACTACAATCCATGTCCAGCCAACGTGGCTGAGACTGTAATTGCTGAGACCAAGGCCAAGAACGCTG
CCAAGAAGTAA

Upstream 100 bases:

>100_bases
TTCCCAATTACAATATGCGCCTCAAATCCTGATGTGTGGTCTGCTGCTTTTTTGTGGCATACTCCGTCTACTTTTATAAA
CCAAGCAAAAAAGTTGAATC

Downstream 100 bases:

>100_bases
TTAATACTTCGGTCCATAAAAACCCGCCTATGTGGCGGGTTTTTTTATGCCTGTAATTTGGGTTATTCAGTTTGAATCAA
GCATGGCGGCTTAGACTGTT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G 2 [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGASTMDFMDQEAERGITIQSAATTCFWKGHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARLIFVNKLDRIGADFLRVVGQVKKVLAANPLVMT
LPIGREDEFTGVVDVLNRQAFIWDDSGLPENYTVTDVPADMVDLVEEYREMLVETAVEQDDDLMEAYMEGEEPSIEDLKR
CIRKGTINLSFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPETGEETGKVATVSVDEPLRALAFKIMDDRF
GALTFVRIYSGKLKKGDTILNSATGKTERIGRMVEMHANDRNEIDSAQAGDIIAVVGMKNVQTGHTLCDPKHECTLEPMI
FPTPVISIAVTPKDKGASEKLGVALGKMVAEDPSFQVETDQETGDTILKGMGELHLDIKVDILKRTFGVELTVGAPQVAY
RETITAAIEDSYTHKKQSGGSGQYGKIDYRIRPGEPGTGFTFKSAVVGGNVPREFWPAVESGFEEMMSQGPLAGFPVLDV
EVELFDGAYHAVDSSAIAFEIAAKGAFRQSMPKAKPQLLEPIMKVDVFTPDDHVGDVIGDLNRRRGMIKDQEAGLTGVRV
KADVPLAEMFGYIGHLRTMTSGRGQFSMEFSHYNPCPANVAETVIAETKAKNAAKK

Sequences:

>Translated_696_residues
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGASTMDFMDQEAERGITIQSAATTCFWKGHRFNVIDTP
GHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARLIFVNKLDRIGADFLRVVGQVKKVLAANPLVMT
LPIGREDEFTGVVDVLNRQAFIWDDSGLPENYTVTDVPADMVDLVEEYREMLVETAVEQDDDLMEAYMEGEEPSIEDLKR
CIRKGTINLSFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPETGEETGKVATVSVDEPLRALAFKIMDDRF
GALTFVRIYSGKLKKGDTILNSATGKTERIGRMVEMHANDRNEIDSAQAGDIIAVVGMKNVQTGHTLCDPKHECTLEPMI
FPTPVISIAVTPKDKGASEKLGVALGKMVAEDPSFQVETDQETGDTILKGMGELHLDIKVDILKRTFGVELTVGAPQVAY
RETITAAIEDSYTHKKQSGGSGQYGKIDYRIRPGEPGTGFTFKSAVVGGNVPREFWPAVESGFEEMMSQGPLAGFPVLDV
EVELFDGAYHAVDSSAIAFEIAAKGAFRQSMPKAKPQLLEPIMKVDVFTPDDHVGDVIGDLNRRRGMIKDQEAGLTGVRV
KADVPLAEMFGYIGHLRTMTSGRGQFSMEFSHYNPCPANVAETVIAETKAKNAAKK
>Mature_695_residues
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGASTMDFMDQEAERGITIQSAATTCFWKGHRFNVIDTPG
HVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYANESEVARLIFVNKLDRIGADFLRVVGQVKKVLAANPLVMTL
PIGREDEFTGVVDVLNRQAFIWDDSGLPENYTVTDVPADMVDLVEEYREMLVETAVEQDDDLMEAYMEGEEPSIEDLKRC
IRKGTINLSFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPETGEETGKVATVSVDEPLRALAFKIMDDRFG
ALTFVRIYSGKLKKGDTILNSATGKTERIGRMVEMHANDRNEIDSAQAGDIIAVVGMKNVQTGHTLCDPKHECTLEPMIF
PTPVISIAVTPKDKGASEKLGVALGKMVAEDPSFQVETDQETGDTILKGMGELHLDIKVDILKRTFGVELTVGAPQVAYR
ETITAAIEDSYTHKKQSGGSGQYGKIDYRIRPGEPGTGFTFKSAVVGGNVPREFWPAVESGFEEMMSQGPLAGFPVLDVE
VELFDGAYHAVDSSAIAFEIAAKGAFRQSMPKAKPQLLEPIMKVDVFTPDDHVGDVIGDLNRRRGMIKDQEAGLTGVRVK
ADVPLAEMFGYIGHLRTMTSGRGQFSMEFSHYNPCPANVAETVIAETKAKNAAKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=691, Percent_Identity=44.1389290882779, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI19923640, Length=713, Percent_Identity=37.4474053295933, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI25306287, Length=688, Percent_Identity=35.4651162790698, Blast_Score=360, Evalue=3e-99,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=42.7848101265823, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=37.7358490566038, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI4503483, Length=503, Percent_Identity=24.4532803180915, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=33.8129496402878, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI1789738, Length=696, Percent_Identity=55.0287356321839, Blast_Score=768, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=516, Percent_Identity=26.5503875968992, Blast_Score=135, Evalue=1e-32,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=35.5191256830601, Blast_Score=100, Evalue=6e-22,
Organism=Escherichia coli, GI48994988, Length=145, Percent_Identity=38.6206896551724, Blast_Score=96, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=696, Percent_Identity=39.5114942528736, Blast_Score=507, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17556745, Length=685, Percent_Identity=29.9270072992701, Blast_Score=274, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=40.1315789473684, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17506493, Length=498, Percent_Identity=24.4979919678715, Blast_Score=101, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323098, Length=700, Percent_Identity=42.8571428571429, Blast_Score=573, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6322359, Length=782, Percent_Identity=31.074168797954, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6324707, Length=418, Percent_Identity=25.8373205741627, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=418, Percent_Identity=25.8373205741627, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=35.0993377483444, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=153, Percent_Identity=31.3725490196078, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=692, Percent_Identity=41.0404624277457, Blast_Score=539, Evalue=1e-153,
Organism=Drosophila melanogaster, GI221458488, Length=724, Percent_Identity=32.7348066298343, Blast_Score=358, Evalue=8e-99,
Organism=Drosophila melanogaster, GI24585711, Length=501, Percent_Identity=24.5508982035928, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585713, Length=501, Percent_Identity=24.5508982035928, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585709, Length=501, Percent_Identity=24.5508982035928, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI78706572, Length=182, Percent_Identity=35.1648351648352, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574573, Length=141, Percent_Identity=34.0425531914894, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 76218; Mature: 76086

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGASTMDFMDQEAERGITI
CCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEE
QSAATTCFWKGHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EECCCEEEECCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARLIFVNKLDRIGADFLRVVGQVKKVLAANPLVMTLPIGREDEFTGVVDVLNRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCE
FIWDDSGLPENYTVTDVPADMVDLVEEYREMLVETAVEQDDDLMEAYMEGEEPSIEDLKR
EEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
CIRKGTINLSFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPETGEETGKVA
HHHHCCCEEEECHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
TVSVDEPLRALAFKIMDDRFGALTFVRIYSGKLKKGDTILNSATGKTERIGRMVEMHAND
EEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHEECCCC
RNEIDSAQAGDIIAVVGMKNVQTGHTLCDPKHECTLEPMIFPTPVISIAVTPKDKGASEK
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCCCCCCHH
LGVALGKMVAEDPSFQVETDQETGDTILKGMGELHLDIKVDILKRTFGVELTVGAPQVAY
HHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEEEEEEEHHHCCEEEEECCCCHHH
RETITAAIEDSYTHKKQSGGSGQYGKIDYRIRPGEPGTGFTFKSAVVGGNVPREFWPAVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEHHCCCCCCCHHHHHHHH
SGFEEMMSQGPLAGFPVLDVEVELFDGAYHAVDSSAIAFEIAAKGAFRQSMPKAKPQLLE
HHHHHHHHCCCCCCCCEEEEEEEEECCHHHHCCCCCEEEEEHHCCHHHHCCCCCCHHHHH
PIMKVDVFTPDDHVGDVIGDLNRRRGMIKDQEAGLTGVRVKADVPLAEMFGYIGHLRTMT
HHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHC
SGRGQFSMEFSHYNPCPANVAETVIAETKAKNAAKK
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TDLSKYRNIGIFAHVDAGKTTTTERILKLTGKIHKLGEVHDGASTMDFMDQEAERGITI
CCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCEEE
QSAATTCFWKGHRFNVIDTPGHVDFTVEVYRSLKVLDGGIGVFCGSGGVEPQSETNWRYA
EECCCEEEECCCEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCC
NESEVARLIFVNKLDRIGADFLRVVGQVKKVLAANPLVMTLPIGREDEFTGVVDVLNRQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCE
FIWDDSGLPENYTVTDVPADMVDLVEEYREMLVETAVEQDDDLMEAYMEGEEPSIEDLKR
EEECCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHH
CIRKGTINLSFFPTFCGSAFKNKGMQLVLDAVVDYLPSPTEVEPQPLTDPETGEETGKVA
HHHHCCCEEEECHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEE
TVSVDEPLRALAFKIMDDRFGALTFVRIYSGKLKKGDTILNSATGKTERIGRMVEMHAND
EEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHCCCCCCHHHHHHHHEECCCC
RNEIDSAQAGDIIAVVGMKNVQTGHTLCDPKHECTLEPMIFPTPVISIAVTPKDKGASEK
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEECCEECCCCEEEEEECCCCCCCCHH
LGVALGKMVAEDPSFQVETDQETGDTILKGMGELHLDIKVDILKRTFGVELTVGAPQVAY
HHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCEEEEEEEEEEHHHCCEEEEECCCCHHH
RETITAAIEDSYTHKKQSGGSGQYGKIDYRIRPGEPGTGFTFKSAVVGGNVPREFWPAVE
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCEEEHHCCCCCCCHHHHHHHH
SGFEEMMSQGPLAGFPVLDVEVELFDGAYHAVDSSAIAFEIAAKGAFRQSMPKAKPQLLE
HHHHHHHHCCCCCCCCEEEEEEEEECCHHHHCCCCCEEEEEHHCCHHHHCCCCCCHHHHH
PIMKVDVFTPDDHVGDVIGDLNRRRGMIKDQEAGLTGVRVKADVPLAEMFGYIGHLRTMT
HHHHHEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHC
SGRGQFSMEFSHYNPCPANVAETVIAETKAKNAAKK
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]