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The map label for this gene is folE

Identifier: 119773463

GI number: 119773463

Start: 389155

End: 389805

Strand: Reverse

Name: folE

Synonym: Sama_0322

Alternate gene names: 119773463

Gene position: 389805-389155 (Counterclockwise)

Preceding gene: 119773464

Following gene: 119773460

Centisome position: 9.05

GC content: 55.76

Gene sequence:

>651_bases
ATGGCACTCAGCGAAGCAGCACAAAAGGTTCAGGCGGCGCTCTTGGAGCGGGGGCTGGAAACTCCCATGGTTCCCTGCGG
ACTCTCCCGCGAGGAACGCAAAGACAAAATCGAACAGCATATGCGGGCAATTCTTGGCCTGATGTCACTCGATTTGACCG
ACGACAGCCTTGCCGATACCCCCCGTCGCATCGCCAAAATGTACGTGGATGAAATCTTCTCTGGCCTCGACTACGAGAAC
TTCCCCAAAATCACTGTCATCGACAACAAGATGGGCGTGGATGAAATGGTACGGGTACAGGATATCAGCCTGACCAGCAC
CTGCGAACACCATTTGGTGACCATAGACGGCACTGCCACAGTGGCCTATCTGCCGCGTAAAAAAATCATCGGCCTGTCGA
AAATCAACCGTATCGTGCGCTTCTTTGCCCAGCGCCCTCAGGTGCAGGAACGCCTGACTCAACAGGTTCTGGTGGCACTG
CAGGCACTGCTGGAAACCAAAGATGTGGCGGTGAAAATCGATGCGGTGCACTACTGCGTTAAATCCCGCGGCGTGATGGA
TGCCACCAGCTCCACCACCACCACGGCACTGGGTGGCATTTTCAAATCCAACCCGGCCACCCGGGCGGAATTTTTACATC
AGCCCCGCTGA

Upstream 100 bases:

>100_bases
CGCCAAATAGCGGTATCCTGCGCCACTCGCGCAATACCCAAGTAATTTGCCCTGATATTGCGCAGCGGCCAAGGCGCCGA
TGATGTGGTTGGAGGTAAAA

Downstream 100 bases:

>100_bases
ACTTAAGCGTATTCCCAATAAAAAACGCCGCTGATTGCGGCGTTTTTTATGCTTAAGCCAAGGGCCCAAACTGGCGGGCG
CTTACCTTTTATGGCTTGGG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MALSEAAQKVQAALLERGLETPMVPCGLSREERKDKIEQHMRAILGLMSLDLTDDSLADTPRRIAKMYVDEIFSGLDYEN
FPKITVIDNKMGVDEMVRVQDISLTSTCEHHLVTIDGTATVAYLPRKKIIGLSKINRIVRFFAQRPQVQERLTQQVLVAL
QALLETKDVAVKIDAVHYCVKSRGVMDATSSTTTTALGGIFKSNPATRAEFLHQPR

Sequences:

>Translated_216_residues
MALSEAAQKVQAALLERGLETPMVPCGLSREERKDKIEQHMRAILGLMSLDLTDDSLADTPRRIAKMYVDEIFSGLDYEN
FPKITVIDNKMGVDEMVRVQDISLTSTCEHHLVTIDGTATVAYLPRKKIIGLSKINRIVRFFAQRPQVQERLTQQVLVAL
QALLETKDVAVKIDAVHYCVKSRGVMDATSSTTTTALGGIFKSNPATRAEFLHQPR
>Mature_215_residues
ALSEAAQKVQAALLERGLETPMVPCGLSREERKDKIEQHMRAILGLMSLDLTDDSLADTPRRIAKMYVDEIFSGLDYENF
PKITVIDNKMGVDEMVRVQDISLTSTCEHHLVTIDGTATVAYLPRKKIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQ
ALLETKDVAVKIDAVHYCVKSRGVMDATSSTTTTALGGIFKSNPATRAEFLHQPR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=119, Percent_Identity=47.8991596638655, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI4503949, Length=119, Percent_Identity=47.8991596638655, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI66932970, Length=98, Percent_Identity=44.8979591836735, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI66932972, Length=82, Percent_Identity=50, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1788476, Length=217, Percent_Identity=68.6635944700461, Blast_Score=288, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17560486, Length=174, Percent_Identity=40.8045977011494, Blast_Score=126, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6321706, Length=182, Percent_Identity=40.1098901098901, Blast_Score=126, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24656782, Length=187, Percent_Identity=40.1069518716578, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24656796, Length=187, Percent_Identity=40.1069518716578, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24656791, Length=187, Percent_Identity=40.1069518716578, Blast_Score=117, Evalue=7e-27,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_SHEAM (A1S2C7)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926203.1
- ProteinModelPortal:   A1S2C7
- SMR:   A1S2C7
- STRING:   A1S2C7
- GeneID:   4602578
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0322
- NMPDR:   fig|326297.7.peg.307
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   MAPIIGR
- PhylomeDB:   A1S2C7
- ProtClustDB:   PRK09347
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 24083; Mature: 23952

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSEAAQKVQAALLERGLETPMVPCGLSREERKDKIEQHMRAILGLMSLDLTDDSLADT
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
PRRIAKMYVDEIFSGLDYENFPKITVIDNKMGVDEMVRVQDISLTSTCEHHLVTIDGTAT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCCCEEEEECCCEE
VAYLPRKKIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLETKDVAVKIDAVHYCV
EEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHH
KSRGVMDATSSTTTTALGGIFKSNPATRAEFLHQPR
HHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure 
ALSEAAQKVQAALLERGLETPMVPCGLSREERKDKIEQHMRAILGLMSLDLTDDSLADT
CHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
PRRIAKMYVDEIFSGLDYENFPKITVIDNKMGVDEMVRVQDISLTSTCEHHLVTIDGTAT
HHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHCCHHHCCCCEEEEECCCEE
VAYLPRKKIIGLSKINRIVRFFAQRPQVQERLTQQVLVALQALLETKDVAVKIDAVHYCV
EEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHH
KSRGVMDATSSTTTTALGGIFKSNPATRAEFLHQPR
HHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA