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The map label for this gene is epsE [H]

Identifier: 119773311

GI number: 119773311

Start: 200369

End: 201931

Strand: Direct

Name: epsE [H]

Synonym: Sama_0170

Alternate gene names: 119773311

Gene position: 200369-201931 (Clockwise)

Preceding gene: 119773310

Following gene: 119773312

Centisome position: 4.65

GC content: 56.56

Gene sequence:

>1563_bases
ATGAGTGACATCAATCAGGGCGAAGTGCTGGAACGCCTGTCCGATGAACTTGGCGTAGAGGCCGAGACCCAGGTAGACGA
GGCGTTTCGCTCCAATAGCCGCGAGCGACTGCCCTTTGCCTTTGCCCACCGTTTTGGCTTGGTGCTCGCCAAAGACGAAC
AGGGTGTGCTGGGGCTGTATTACACAGACAAAACACCGCTGGCCGCCTTGTTGGAAGTGCGCCGCTACGCCGGTGAAGCC
GTGCCATTGCAACGACTGGAAGCTTCGGCCTTCGAGGCCAGACTGACCCAGGCGTATCAGGCCAATTCTTCTGAAGCGCA
GCAGCTGATGGAAGATATTGGCAATGAGATGGACCTCTTCACCCTGGCCGAAGAGCTGCCTCAGACCGAAGATCTGCTGG
AAGGCGACGATGATGCGCCCATTATCCGGCTTATCAACGCGCTCCTGTCGGAAGCCATTAAAGAAGAGGCGTCGGACATC
CACATCGAGACTTACGAGAAGCAGCTCGTGGTGCGTTTCCGGGTGGATGGGGTGCTCAAAGAAGTGCTCAAACCCAACCG
TAAGCTTTCGTCACTGCTGGTCAGCCGTATCAAGGTAATGGCTCGTCTGGACATCGCAGAAAAACGCGTGCCACAGGATG
GCCGTATCTCATTGCGTATCGGTGGCCGAGCCGTGGATGTGCGGGTGTCGACCATGCCATCGAGCCACGGTGAACGGGTG
GTACTGCGTCTGCTCGATAAAAATGCCGGTAATCTGGATTTGGCTCAGCTTGGGATGACTGAAACCATTCGCGAGCAATT
CGATGAGCTTATCCGCAAGCCCCACGGTATCCTGCTGGTGACTGGTCCTACAGGTTCGGGTAAGAGTACGACTCTCTACG
CGGGCCTCACCGAAATCAATTCCCGTGACACCAACATTCTCACGGTGGAAGACCCCATCGAATATGAGCTCGAAGGTATA
GGTCAAACCCAGGTCAACACCAAGGTCGACATGACTTTTGCCCGCGGCCTGCGTGCCATTTTGCGTCAGGACCCTGATGT
GGTGATGATTGGGGAAATCCGGGACCTGGAAACGGCTCAAATCGCGGTGCAGGCGTCCCTTACGGGTCACCTGGTGATTT
CTACTCTGCACACCAACACTGCATCCGGTGCTATTACTCGATTGCAGGATATGGGGGTTGAGCCCTTCCTGGTGTCGTCC
AGTTTGCTCGGTGTATTGGCCCAGCGACTGGTACGGACCCTGTGCCCAAGCTGTAAGGCGCCCCATGTGCCCGATGAGCG
AGAGCGTGAACTGCTGGGTATTGGCGGTGGTGATGCGGTTATCTACCGCGCCACCGGCTGTAAGGCCTGTGGCCATAATG
GTTATCGCGGTCGTACCGGTATCCACGAGCTGCTGTTGGTGGATGACACTGTGCGTGAACTTATCCATGGTGGCAGGGGC
GAGCTGGCGATTGAGAAATACATTCGTCAATCGGTTCCCAGCATTCGCCACGATGGTATGCAAAAGGTCCTGGCGGGCAT
TACCACCCTCGAAGAAGTACTGCGGGTGACCCGCGAGGAGTAA

Upstream 100 bases:

>100_bases
ATCCTTGACCGCTACAAAGAAGGCAAGGGGTTGGACACCGACATGCGTGAAAAGGACGCCGCACTGAATTACATCAACGA
GAACAAGTAACGGGCGGCCT

Downstream 100 bases:

>100_bases
GGCATGCCGGCTTTTGAATACAAGGCGCTCGATGGCAAGGGCAAACAGCAAAAAGGGGTGATTGAGGCAGACAGTGCCCG
TCATGCCCGCAGTCAGCTGC

Product: general secretion pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSDINQGEVLERLSDELGVEAETQVDEAFRSNSRERLPFAFAHRFGLVLAKDEQGVLGLYYTDKTPLAALLEVRRYAGEA
VPLQRLEASAFEARLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIRLINALLSEAIKEEASDI
HIETYEKQLVVRFRVDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLAQLGMTETIREQFDELIRKPHGILLVTGPTGSGKSTTLYAGLTEINSRDTNILTVEDPIEYELEGI
GQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLVRTLCPSCKAPHVPDERERELLGIGGGDAVIYRATGCKACGHNGYRGRTGIHELLLVDDTVRELIHGGRG
ELAIEKYIRQSVPSIRHDGMQKVLAGITTLEEVLRVTREE

Sequences:

>Translated_520_residues
MSDINQGEVLERLSDELGVEAETQVDEAFRSNSRERLPFAFAHRFGLVLAKDEQGVLGLYYTDKTPLAALLEVRRYAGEA
VPLQRLEASAFEARLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIRLINALLSEAIKEEASDI
HIETYEKQLVVRFRVDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERV
VLRLLDKNAGNLDLAQLGMTETIREQFDELIRKPHGILLVTGPTGSGKSTTLYAGLTEINSRDTNILTVEDPIEYELEGI
GQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSS
SLLGVLAQRLVRTLCPSCKAPHVPDERERELLGIGGGDAVIYRATGCKACGHNGYRGRTGIHELLLVDDTVRELIHGGRG
ELAIEKYIRQSVPSIRHDGMQKVLAGITTLEEVLRVTREE
>Mature_519_residues
SDINQGEVLERLSDELGVEAETQVDEAFRSNSRERLPFAFAHRFGLVLAKDEQGVLGLYYTDKTPLAALLEVRRYAGEAV
PLQRLEASAFEARLTQAYQANSSEAQQLMEDIGNEMDLFTLAEELPQTEDLLEGDDDAPIIRLINALLSEAIKEEASDIH
IETYEKQLVVRFRVDGVLKEVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERVV
LRLLDKNAGNLDLAQLGMTETIREQFDELIRKPHGILLVTGPTGSGKSTTLYAGLTEINSRDTNILTVEDPIEYELEGIG
QTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSS
LLGVLAQRLVRTLCPSCKAPHVPDERERELLGIGGGDAVIYRATGCKACGHNGYRGRTGIHELLLVDDTVRELIHGGRGE
LAIEKYIRQSVPSIRHDGMQKVLAGITTLEEVLRVTREE

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=484, Percent_Identity=58.4710743801653, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=43.0809399477807, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082188, Length=169, Percent_Identity=45.5621301775148, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 57393; Mature: 57262

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINQGEVLERLSDELGVEAETQVDEAFRSNSRERLPFAFAHRFGLVLAKDEQGVLGLY
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCEEEEECCCCEEEEE
YTDKTPLAALLEVRRYAGEAVPLQRLEASAFEARLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIRLINALLSEAIKEEASDIHIETYEKQLVVRFRVDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLAQLGMTETIREQFDELIRKPHGILLVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SRDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLVRTLCPSCKA
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PHVPDERERELLGIGGGDAVIYRATGCKACGHNGYRGRTGIHELLLVDDTVRELIHGGRG
CCCCCHHCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHEEECHHHHHHHHCCCC
ELAIEKYIRQSVPSIRHDGMQKVLAGITTLEEVLRVTREE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDINQGEVLERLSDELGVEAETQVDEAFRSNSRERLPFAFAHRFGLVLAKDEQGVLGLY
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHCEEEEECCCCEEEEE
YTDKTPLAALLEVRRYAGEAVPLQRLEASAFEARLTQAYQANSSEAQQLMEDIGNEMDLF
EECCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
TLAEELPQTEDLLEGDDDAPIIRLINALLSEAIKEEASDIHIETYEKQLVVRFRVDGVLK
HHHHHCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHHHHH
EVLKPNRKLSSLLVSRIKVMARLDIAEKRVPQDGRISLRIGGRAVDVRVSTMPSSHGERV
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCCHHH
VLRLLDKNAGNLDLAQLGMTETIREQFDELIRKPHGILLVTGPTGSGKSTTLYAGLTEIN
HHHHHCCCCCCCCHHHHCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHCC
SRDTNILTVEDPIEYELEGIGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQ
CCCCEEEEECCCCCHHHCCCCCEECCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
IAVQASLTGHLVISTLHTNTASGAITRLQDMGVEPFLVSSSLLGVLAQRLVRTLCPSCKA
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
PHVPDERERELLGIGGGDAVIYRATGCKACGHNGYRGRTGIHELLLVDDTVRELIHGGRG
CCCCCHHCCCEEECCCCCEEEEEECCCCCCCCCCCCCCCCHHHHEEECHHHHHHHHCCCC
ELAIEKYIRQSVPSIRHDGMQKVLAGITTLEEVLRVTREE
CHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]