Click here to switch to the map view.

The map label for this gene is epsD [H]

Identifier: 119773310

GI number: 119773310

Start: 198247

End: 200358

Strand: Direct

Name: epsD [H]

Synonym: Sama_0169

Alternate gene names: 119773310

Gene position: 198247-200358 (Clockwise)

Preceding gene: 119773309

Following gene: 119773311

Centisome position: 4.6

GC content: 54.12

Gene sequence:

>2112_bases
ATGAACAATCAAGGATTCCGACGCAAACTGATCGCCAGCCTGGTCGCTGGTACAGTGCTCCTTTCGCCTGCGATTGCCTG
GTCTGCTTCCAACGAGCAGCTGGCGCCAAGTTTCAAAAATGCCGAAATTCAGGAATTTATTAATACTGTTGGTAAACGAC
TGAACAAGACCATCATTGTCGATCCGACCATCCGCGGCAAAATCAACGTCAGAAGTTACGATAACCTCACCGAGGACCAA
TACTTCCAGTTTTTCCTGAACGTGCTCCAGGTGTATGGCTATGCTGTGGTGGAAATGGAAAATGGCGTCCTTAAGGTCAT
TAAAGACAAGGATGCCAAGACCTCCAACATCCGTGTAGCAGATGACAGTGCCCCGGGCTTGGGAGATGAATTGGTCACCC
GCATCGTGCCCCTGTACAACTCCGAAGCCAAGCAGCTGGCACCGCTTTTGCGTCAGCTTAACGACAACGCCGGTGGCGGT
AACGTGGTTAACTACGACCCGTCCAACGTACTGATGCTCACCGGCCGTGCTGCCGTGGTGAACAAGTTGGTGGAAATCGT
TAAACGTGTAGATAAACAGGGCGATACCGAAGTTCTGGTCGTGCCTTTGCAGCACGCCTCCGCTTCTGAAATGGTGCGGA
TTATCGATACCCTCTACCGCGCTTCCGCCAACCAGGCACAGCTGCCCGGTCAGGCCCCCAAGGTGGTGGCTGATGAGCGT
ATTAACGCCGTGGTGATCAGTGGCGATGAAAAGAGTCGCCAGCGTGTAGTGGAATTGGTGAAACGTCTGGACGCCGAGCA
GGCCAGCACAGGCAACACCAAGGTGCGTTACCTGCGTTATGCCAAGGCCGAAGATTTGGTTGAAGTGCTGACCGGCTTTG
CCCAAAAACTGGAAGAAAGCAAAGACAACGGCGCACAGCAAGCTTCGGGCAAGCGTCGCAGCCAAATCAATATCATGGCG
CACACAGACACCAATGCTCTGGTCATCAGCGCCGAGCCTGACCAGATGCGTACCCTAGAAAGCGTCATCAATCAGCTCGA
TATCCGCCGTGCTCAGGTGCTGGTGGAAGCCATCATTGTGGAAGTGGCAGAAGGTGACGATGTGGGCTTTGGTGTGCAGT
GGGCCACTGAGTCCGGTGGCGGCACCCAATTCAATAATCTGGGCCCTACCATTGGTGAAATTGGCGCCGGTATCTGGCAG
GCCCGGGGCGAAGAAGCCTCCGAAGTGACCACCATTGACGGCAACGGTAACCCAATCGTTTCCAAGAACCCTGAAAAACG
TGGTGATATCACCTTGCTGGCACAGGCGCTGGGCAAGGTGAATGGCATGGCGTGGGGTGTGGCCATGGGCGACTTTGGTG
CCCTGATTCAGGCCGTGTCCTCCGATACCAACTCAAACGTGCTGGCTACGCCTTCCATCACCACTCTGGATAACCAGGAA
GCTGCCTTTATCGTGGGTGATGAGGTGCCGGTACTGACAGGGGCGCAAAACTCCAGCAACGGTAACTCCAACCCCTTCCA
GACGGTTGAACGTAAGGAAGTGGGTGTGAAGCTGAAAGTGACGCCACAAATCAACGAAGGCAATGCAGTGAAGCTGGCCA
TTGAGCAGGAAGTGTCTGGTATCAATGGCAAGACGGGCGTAGACGTGACCTTCGCTACCCGCCGTTTGACCACCACAGTT
ATGGCTGACTCCGGCCAAATCGTGGTGCTGGGTGGGTTGATCAACGAAGAGGTGCAGGAGAGCGTGCAGAAGGTGCCTAT
CCTGGGCGATATTCCGCTGCTTGGCCATCTGTTCAAGTCGTCTTCCAGCGGTAAGAAAAAGAAAAACCTGATGGTGTTTA
TCAAACCCACCATCATTCGTGATGGCATTACCATGGAAGGCATCGCCGGTCGCAAATACAACTATTTCCGTGCCCTGCAG
CTCGAGCAACAGGAACGTGGCGTTAATCTGATGCCCAATACCAGTGTGCCCGTGCTTGAAGAGTGGGATCAGTCGCAGCA
TCTGCCACCGGAAGTGAATGACATCCTTGACCGCTACAAAGAAGGCAAGGGGTTGGACACCGACATGCGTGAAAAGGACG
CCGCACTGAATTACATCAACGAGAACAAGTAA

Upstream 100 bases:

>100_bases
AACTGACCGAAATCTCCGTCATGGTAGAAAGGGAAGGTCAGCTGATTGAAATCATGTTCAGTTTGCCGCAATAAGCGAAG
TTTTAGGGGACAATAATTAG

Downstream 100 bases:

>100_bases
CGGGCGGCCTATGAGTGACATCAATCAGGGCGAAGTGCTGGAACGCCTGTCCGATGAACTTGGCGTAGAGGCCGAGACCC
AGGTAGACGAGGCGTTTCGC

Product: type II and III secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MNNQGFRRKLIASLVAGTVLLSPAIAWSASNEQLAPSFKNAEIQEFINTVGKRLNKTIIVDPTIRGKINVRSYDNLTEDQ
YFQFFLNVLQVYGYAVVEMENGVLKVIKDKDAKTSNIRVADDSAPGLGDELVTRIVPLYNSEAKQLAPLLRQLNDNAGGG
NVVNYDPSNVLMLTGRAAVVNKLVEIVKRVDKQGDTEVLVVPLQHASASEMVRIIDTLYRASANQAQLPGQAPKVVADER
INAVVISGDEKSRQRVVELVKRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEESKDNGAQQASGKRRSQINIMA
HTDTNALVISAEPDQMRTLESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATESGGGTQFNNLGPTIGEIGAGIWQ
ARGEEASEVTTIDGNGNPIVSKNPEKRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTNSNVLATPSITTLDNQE
AAFIVGDEVPVLTGAQNSSNGNSNPFQTVERKEVGVKLKVTPQINEGNAVKLAIEQEVSGINGKTGVDVTFATRRLTTTV
MADSGQIVVLGGLINEEVQESVQKVPILGDIPLLGHLFKSSSSGKKKKNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQ
LEQQERGVNLMPNTSVPVLEEWDQSQHLPPEVNDILDRYKEGKGLDTDMREKDAALNYINENK

Sequences:

>Translated_703_residues
MNNQGFRRKLIASLVAGTVLLSPAIAWSASNEQLAPSFKNAEIQEFINTVGKRLNKTIIVDPTIRGKINVRSYDNLTEDQ
YFQFFLNVLQVYGYAVVEMENGVLKVIKDKDAKTSNIRVADDSAPGLGDELVTRIVPLYNSEAKQLAPLLRQLNDNAGGG
NVVNYDPSNVLMLTGRAAVVNKLVEIVKRVDKQGDTEVLVVPLQHASASEMVRIIDTLYRASANQAQLPGQAPKVVADER
INAVVISGDEKSRQRVVELVKRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEESKDNGAQQASGKRRSQINIMA
HTDTNALVISAEPDQMRTLESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATESGGGTQFNNLGPTIGEIGAGIWQ
ARGEEASEVTTIDGNGNPIVSKNPEKRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTNSNVLATPSITTLDNQE
AAFIVGDEVPVLTGAQNSSNGNSNPFQTVERKEVGVKLKVTPQINEGNAVKLAIEQEVSGINGKTGVDVTFATRRLTTTV
MADSGQIVVLGGLINEEVQESVQKVPILGDIPLLGHLFKSSSSGKKKKNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQ
LEQQERGVNLMPNTSVPVLEEWDQSQHLPPEVNDILDRYKEGKGLDTDMREKDAALNYINENK
>Mature_703_residues
MNNQGFRRKLIASLVAGTVLLSPAIAWSASNEQLAPSFKNAEIQEFINTVGKRLNKTIIVDPTIRGKINVRSYDNLTEDQ
YFQFFLNVLQVYGYAVVEMENGVLKVIKDKDAKTSNIRVADDSAPGLGDELVTRIVPLYNSEAKQLAPLLRQLNDNAGGG
NVVNYDPSNVLMLTGRAAVVNKLVEIVKRVDKQGDTEVLVVPLQHASASEMVRIIDTLYRASANQAQLPGQAPKVVADER
INAVVISGDEKSRQRVVELVKRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEESKDNGAQQASGKRRSQINIMA
HTDTNALVISAEPDQMRTLESVINQLDIRRAQVLVEAIIVEVAEGDDVGFGVQWATESGGGTQFNNLGPTIGEIGAGIWQ
ARGEEASEVTTIDGNGNPIVSKNPEKRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTNSNVLATPSITTLDNQE
AAFIVGDEVPVLTGAQNSSNGNSNPFQTVERKEVGVKLKVTPQINEGNAVKLAIEQEVSGINGKTGVDVTFATRRLTTTV
MADSGQIVVLGGLINEEVQESVQKVPILGDIPLLGHLFKSSSSGKKKKNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQ
LEQQERGVNLMPNTSVPVLEEWDQSQHLPPEVNDILDRYKEGKGLDTDMREKDAALNYINENK

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=668, Percent_Identity=46.2574850299401, Blast_Score=560, Evalue=1e-160,
Organism=Escherichia coli, GI1789793, Length=304, Percent_Identity=24.6710526315789, Blast_Score=76, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 76381; Mature: 76381

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQGFRRKLIASLVAGTVLLSPAIAWSASNEQLAPSFKNAEIQEFINTVGKRLNKTIIV
CCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
DPTIRGKINVRSYDNLTEDQYFQFFLNVLQVYGYAVVEMENGVLKVIKDKDAKTSNIRVA
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEE
DDSAPGLGDELVTRIVPLYNSEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLTGRAAVV
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHH
NKLVEIVKRVDKQGDTEVLVVPLQHASASEMVRIIDTLYRASANQAQLPGQAPKVVADER
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC
INAVVISGDEKSRQRVVELVKRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEES
CCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
KDNGAQQASGKRRSQINIMAHTDTNALVISAEPDQMRTLESVINQLDIRRAQVLVEAIIV
HCCCHHHCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAEGDDVGFGVQWATESGGGTQFNNLGPTIGEIGAGIWQARGEEASEVTTIDGNGNPIV
HHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCHHHHCCCCCCCCEEEECCCCCCCC
SKNPEKRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTNSNVLATPSITTLDNQE
CCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCC
AAFIVGDEVPVLTGAQNSSNGNSNPFQTVERKEVGVKLKVTPQINEGNAVKLAIEQEVSG
EEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECEECCCCEEEEEEECCCCC
INGKTGVDVTFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPILGDIPLLGHLFKS
CCCCCCCEEEEEHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHC
SSSGKKKKNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVNLMPNTSVPVLE
CCCCCCCCCEEEEEEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHH
EWDQSQHLPPEVNDILDRYKEGKGLDTDMREKDAALNYINENK
HCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNQGFRRKLIASLVAGTVLLSPAIAWSASNEQLAPSFKNAEIQEFINTVGKRLNKTIIV
CCCCHHHHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEE
DPTIRGKINVRSYDNLTEDQYFQFFLNVLQVYGYAVVEMENGVLKVIKDKDAKTSNIRVA
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCCCCEEEEE
DDSAPGLGDELVTRIVPLYNSEAKQLAPLLRQLNDNAGGGNVVNYDPSNVLMLTGRAAVV
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCHHHH
NKLVEIVKRVDKQGDTEVLVVPLQHASASEMVRIIDTLYRASANQAQLPGQAPKVVADER
HHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCC
INAVVISGDEKSRQRVVELVKRLDAEQASTGNTKVRYLRYAKAEDLVEVLTGFAQKLEES
CCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHH
KDNGAQQASGKRRSQINIMAHTDTNALVISAEPDQMRTLESVINQLDIRRAQVLVEAIIV
HCCCHHHCCCCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAEGDDVGFGVQWATESGGGTQFNNLGPTIGEIGAGIWQARGEEASEVTTIDGNGNPIV
HHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCHHHHCCCCCCCCEEEECCCCCCCC
SKNPEKRGDITLLAQALGKVNGMAWGVAMGDFGALIQAVSSDTNSNVLATPSITTLDNQE
CCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCCEEECCCEEECCCCC
AAFIVGDEVPVLTGAQNSSNGNSNPFQTVERKEVGVKLKVTPQINEGNAVKLAIEQEVSG
EEEEECCCCCEEECCCCCCCCCCCCHHHHHHHHCCEEEEEECEECCCCEEEEEEECCCCC
INGKTGVDVTFATRRLTTTVMADSGQIVVLGGLINEEVQESVQKVPILGDIPLLGHLFKS
CCCCCCCEEEEEHHEEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHC
SSSGKKKKNLMVFIKPTIIRDGITMEGIAGRKYNYFRALQLEQQERGVNLMPNTSVPVLE
CCCCCCCCCEEEEEEEEEEECCCEECCCCCCCCCEEEEEEEHHHHCCCCCCCCCCCCHHH
EWDQSQHLPPEVNDILDRYKEGKGLDTDMREKDAALNYINENK
HCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]