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The map label for this gene is rsmB [H]

Identifier: 119773183

GI number: 119773183

Start: 44805

End: 46088

Strand: Reverse

Name: rsmB [H]

Synonym: Sama_0041

Alternate gene names: 119773183

Gene position: 46088-44805 (Counterclockwise)

Preceding gene: 119773184

Following gene: 119773182

Centisome position: 1.07

GC content: 57.55

Gene sequence:

>1284_bases
ATGAATATCCGAGCCCTCGCCGCCAAGGCGATTTTCGATGTGCTGGAGCGTGGCGTGTCTCTGTCGGTGGCTTTGCCTAC
CCAGCAACTTTGGCTTCGTGACGGAAAAGACAAGGCTCTGCTTGCTGAAATCTGTTATGGCGTCATGCGCAGCCTGCCTC
AGCTCGACAAGCTGGTCAGTGGTTATATGCAAAAGCCCCTCAAGGGCAAACAGCGTGTTGTCCACCAGCTGCTATTGGTG
GGTGCCTATCAGCTGTATTTCACCCGGGTGCCGGCCCACGCCGCCATTTCCGAAACCGCCGAAGGCTGTCGCGCCCTCGG
CTTCGAGAGTCTGGTAAAGGTGGTCAACGGGGTGCTGCGCAATATTCAGCGTCAGGTGCCGCCTTTGGATGAGTCCAACG
ACACCTTGAAGTATAACTTTCCGGCCTGGATAATCCGTCGCCTGAAAGACGCCTACCCGGAAACCTGGGAAAACATCATA
GAGGCGTCACACCAACGTCCACCCATGTGGCTTAGAAACAACCGCCGCCACCAGAGCCGTGATGCGTATTTGCAGGCGCT
TAGCGATGCTGAGGTAAGTGCCGTTGCGGGTCCGGGTGAAGATGCCATTTGCCTCGAATTTGCCACCGACGTTGCCCGCC
TGCCGCATTTTGCCGATGGCGCCAGTTCGGTGCAGGACGGCGCAGCCCAGTGGGCCGCAAGCCTGCTGGCCCCCCGAGAT
GGAGAACTGGTACTGGATGCCTGTGCGGCGCCCGGCGGTAAAAGCTGTCATCTGCTGGAGCTTGCCGACATCGAGCTGGT
CGCCGTAGATTTCGACCAGAAACGCCTCGAGCGGGTGTCACAAAACCTCGAGCGACTCAAACTTGATGCCAGGGTCATTC
ACGGCGATGCCGGGGATATCGGCAGTTGGTGGCAGGGCGGCAAGTTTGACCGCATCCTGCTGGATGCGCCCTGCTCTGCG
ACTGGCGTCATCCGCCGCCATCCGGACATCAAATGGCTCAGACAAAACAACGACATTGCCGAGCTTGCCAAACTTCAGCA
GCAAATTCTCGACAACTGCTGGCAGTGGCTTAAGCCTGGTGGCACACTGCTGTACGCAACCTGTTCTATTTTGCCTGAAG
AAAACCGTGAGCAGATCAAGGCTTTCCTCGAACGCACCCCAGATGCGACCCTGGAAACCCTGCCCAATCAGGCAAACAAT
AACGATATTGGCTGGCAAATCCTGCCGGGACAGGACGACATGGACGGGTTTTATTACGCCCGCCTGAAAAAAGCGCTGGT
TTAG

Upstream 100 bases:

>100_bases
TGCCCGGTAAAAAGCCGATGGACGTGGCCGACATCCTCAATAGCCGTGGTGACTGGTTCACTCCCGGCACCCTGCTGGCA
AGCCCAGAGGCAAGTGAGTA

Downstream 100 bases:

>100_bases
GGATTTAGGCAAACGCCATGAAAATTATCATCTTGGGCGCAGGTCAAGTCGGGGGCACCCTCGCAGAAAACCTGGTGGGT
GAAAACAACGACATCACCAT

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNIRALAAKAIFDVLERGVSLSVALPTQQLWLRDGKDKALLAEICYGVMRSLPQLDKLVSGYMQKPLKGKQRVVHQLLLV
GAYQLYFTRVPAHAAISETAEGCRALGFESLVKVVNGVLRNIQRQVPPLDESNDTLKYNFPAWIIRRLKDAYPETWENII
EASHQRPPMWLRNNRRHQSRDAYLQALSDAEVSAVAGPGEDAICLEFATDVARLPHFADGASSVQDGAAQWAASLLAPRD
GELVLDACAAPGGKSCHLLELADIELVAVDFDQKRLERVSQNLERLKLDARVIHGDAGDIGSWWQGGKFDRILLDAPCSA
TGVIRRHPDIKWLRQNNDIAELAKLQQQILDNCWQWLKPGGTLLYATCSILPEENREQIKAFLERTPDATLETLPNQANN
NDIGWQILPGQDDMDGFYYARLKKALV

Sequences:

>Translated_427_residues
MNIRALAAKAIFDVLERGVSLSVALPTQQLWLRDGKDKALLAEICYGVMRSLPQLDKLVSGYMQKPLKGKQRVVHQLLLV
GAYQLYFTRVPAHAAISETAEGCRALGFESLVKVVNGVLRNIQRQVPPLDESNDTLKYNFPAWIIRRLKDAYPETWENII
EASHQRPPMWLRNNRRHQSRDAYLQALSDAEVSAVAGPGEDAICLEFATDVARLPHFADGASSVQDGAAQWAASLLAPRD
GELVLDACAAPGGKSCHLLELADIELVAVDFDQKRLERVSQNLERLKLDARVIHGDAGDIGSWWQGGKFDRILLDAPCSA
TGVIRRHPDIKWLRQNNDIAELAKLQQQILDNCWQWLKPGGTLLYATCSILPEENREQIKAFLERTPDATLETLPNQANN
NDIGWQILPGQDDMDGFYYARLKKALV
>Mature_427_residues
MNIRALAAKAIFDVLERGVSLSVALPTQQLWLRDGKDKALLAEICYGVMRSLPQLDKLVSGYMQKPLKGKQRVVHQLLLV
GAYQLYFTRVPAHAAISETAEGCRALGFESLVKVVNGVLRNIQRQVPPLDESNDTLKYNFPAWIIRRLKDAYPETWENII
EASHQRPPMWLRNNRRHQSRDAYLQALSDAEVSAVAGPGEDAICLEFATDVARLPHFADGASSVQDGAAQWAASLLAPRD
GELVLDACAAPGGKSCHLLELADIELVAVDFDQKRLERVSQNLERLKLDARVIHGDAGDIGSWWQGGKFDRILLDAPCSA
TGVIRRHPDIKWLRQNNDIAELAKLQQQILDNCWQWLKPGGTLLYATCSILPEENREQIKAFLERTPDATLETLPNQANN
NDIGWQILPGQDDMDGFYYARLKKALV

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=324, Percent_Identity=28.7037037037037, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI76150623, Length=324, Percent_Identity=28.7037037037037, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI32698918, Length=178, Percent_Identity=33.7078651685393, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI301336155, Length=189, Percent_Identity=32.8042328042328, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI39995082, Length=189, Percent_Identity=32.8042328042328, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI11545785, Length=179, Percent_Identity=36.3128491620112, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI40316918, Length=197, Percent_Identity=29.4416243654822, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367212, Length=429, Percent_Identity=52.9137529137529, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI87081985, Length=193, Percent_Identity=33.160621761658, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=317, Percent_Identity=28.0757097791798, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25143471, Length=297, Percent_Identity=28.2828282828283, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17509941, Length=160, Percent_Identity=29.375, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=235, Percent_Identity=28.5106382978723, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319447, Length=187, Percent_Identity=31.5508021390374, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI22024126, Length=315, Percent_Identity=28.2539682539683, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=31.6091954022989, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24668781, Length=259, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356513, Length=259, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355201, Length=226, Percent_Identity=29.2035398230088, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47701; Mature: 47701

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRALAAKAIFDVLERGVSLSVALPTQQLWLRDGKDKALLAEICYGVMRSLPQLDKLVS
CCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GYMQKPLKGKQRVVHQLLLVGAYQLYFTRVPAHAAISETAEGCRALGFESLVKVVNGVLR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
NIQRQVPPLDESNDTLKYNFPAWIIRRLKDAYPETWENIIEASHQRPPMWLRNNRRHQSR
HHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHCCCCCHHH
DAYLQALSDAEVSAVAGPGEDAICLEFATDVARLPHFADGASSVQDGAAQWAASLLAPRD
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
GELVLDACAAPGGKSCHLLELADIELVAVDFDQKRLERVSQNLERLKLDARVIHGDAGDI
CCEEEEECCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCC
GSWWQGGKFDRILLDAPCSATGVIRRHPDIKWLRQNNDIAELAKLQQQILDNCWQWLKPG
CCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCC
GTLLYATCSILPEENREQIKAFLERTPDATLETLPNQANNNDIGWQILPGQDDMDGFYYA
CEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEECCCCCCCHHHHH
RLKKALV
HHHHHCC
>Mature Secondary Structure
MNIRALAAKAIFDVLERGVSLSVALPTQQLWLRDGKDKALLAEICYGVMRSLPQLDKLVS
CCHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GYMQKPLKGKQRVVHQLLLVGAYQLYFTRVPAHAAISETAEGCRALGFESLVKVVNGVLR
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
NIQRQVPPLDESNDTLKYNFPAWIIRRLKDAYPETWENIIEASHQRPPMWLRNNRRHQSR
HHHHHCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHHHHHHHCCCCCCHHHHCCCCCHHH
DAYLQALSDAEVSAVAGPGEDAICLEFATDVARLPHFADGASSVQDGAAQWAASLLAPRD
HHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
GELVLDACAAPGGKSCHLLELADIELVAVDFDQKRLERVSQNLERLKLDARVIHGDAGDI
CCEEEEECCCCCCCCEEEEEECCEEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCCCCC
GSWWQGGKFDRILLDAPCSATGVIRRHPDIKWLRQNNDIAELAKLQQQILDNCWQWLKPG
CCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCC
GTLLYATCSILPEENREQIKAFLERTPDATLETLPNQANNNDIGWQILPGQDDMDGFYYA
CEEEEEEEECCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCCCCCEEEECCCCCCCHHHHH
RLKKALV
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]