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The map label for this gene is leuC

Identifier: 119717522

GI number: 119717522

Start: 3503791

End: 3505206

Strand: Reverse

Name: leuC

Synonym: Noca_3298

Alternate gene names: 119717522

Gene position: 3505206-3503791 (Counterclockwise)

Preceding gene: 119717524

Following gene: 119717521

Centisome position: 70.3

GC content: 70.83

Gene sequence:

>1416_bases
ATGGGCAGGACCTTGGCCGAGAAGGTCTGGGACGAGCATGTCGTCCGGTCGACACCGGGGGAGCCGGACCTCCTCTACAT
CGACCTGCACCTGATCCACGAGGTCACCTCCCCGCAGGCCTTCGACGGCCTCCGGCTCGCCGGCCGCACCGTGCGTCGCC
CGGACCTCACGCTGGCCACCGAGGACCACAACGTCCCCACCCTCGACTGGGACAAGCCCATCGCCGACCCGGTCTCGAAG
ACCCAGGTCGACACGCTGCGCCGCAACGCCGCGGAGTTCGGAGTCCGGCTGCACCCCCTCGGCGACGTCGAGCAGGGCAT
CGTGCACGTCGTCGGCCCGCAGCTCGGGCTGACCCAGCCCGGGATGACGATCGTGTGCGGCGACAGCCACACCAGCACGC
ACGGAGCATTCGGCGCGATCGCGTTCGGGATCGGCACCTCGGAGGTCGAGCACGTCCTCGCCACGCAGACGCTGCCGCAG
GCCAAGCCGAAGACGATGGCCGTCACGGTCGAGGGCAGCCTGCCCGACGGCGTGACCGCGAAGGACCTGGTGCTGACCCT
GATCGCCCACACGGGCACCGGTGGCGGGCAGGGCTACATCGTCGAGTACCGCGGCCCGGCCATCGAGGAGCTCTCGATGG
AGGGCCGGATGACCGTCTGCAACATGTCCATCGAGTGGGGGGCCAAGGCCGGCCTGATCGCCCCCGACCAGACGACGTTC
GACTACATCGAGGGCCGGCCGGAGGCGCCGAAGGGCGCCGACTGGGACGCCGCCGTTGCGCACTGGAAGACGCTGGTCAC
CGACGCGGACGCGACGTTCGACAAGGAGATCGTGCTCGACGCGAGCACGATGACGCCGTTCGTCACCTGGGGCACCAATC
CCGGCCAGGGCGTGCCGCTCGGGGGCAGCGTTCCGGACCCGGCGCAGTACGACGACCCCTCGGACCGGATCGCCGCTGAG
AAGGCATGCGAGTACATGGGCCTCGAGGCCGGCACGCCGATGCGCGACATCAAGGTCGACACCGTCTTCATCGGCTCGTG
CACCAACGGCCGGATCGAGGACCTGCGCGCGGCCGCGGAGATCATCAAGGGCCGCCAGGTCGACAAGTCCACCCGGCTGC
TCGTCGTACCGGGCTCGGTGCGCGTGCGTCTCCAGGCCCAGGACGAGGGCCTCGACGTGATCTTCAAGGAGGCCGGCGGC
GAGTGGCGCGGCGCGGGCTGCTCGATGTGCCTGGGCATGAACCCCGACACCCTGCAGCCCGGTGAGCGCAGCGCCTCGAC
GTCCAACCGCAACTTCGAGGGCCGCCAGGGCAAGGGCGGCCGCACCCACCTCGTGTCGGTGCCGGTCGCCGCCGCGACCG
CGATCCGCGGCACCCTGTCCTCGCCCGCCGACCTCGAGCCCGTTGGGAGCAACTGA

Upstream 100 bases:

>100_bases
GCGTCTCAGATCATGGGATGCAAGCGCTAGGATCTGTGATGTTGTCGAACGAGGTGTCGCCGATCCGGGCGGGCACCGTG
AGCTCACGAGGGAGAGCGCC

Downstream 100 bases:

>100_bases
GATGGACAAGTTCACCACCCACACCGGCGTCGGGATCCCGCTGCGGCGCAGCAACGTCGACACCGACCAGATCATCCCGG
CCGTCTACCTCAAGCGGGTC

Product: 3-isopropylmalate dehydratase, large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MGRTLAEKVWDEHVVRSTPGEPDLLYIDLHLIHEVTSPQAFDGLRLAGRTVRRPDLTLATEDHNVPTLDWDKPIADPVSK
TQVDTLRRNAAEFGVRLHPLGDVEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQ
AKPKTMAVTVEGSLPDGVTAKDLVLTLIAHTGTGGGQGYIVEYRGPAIEELSMEGRMTVCNMSIEWGAKAGLIAPDQTTF
DYIEGRPEAPKGADWDAAVAHWKTLVTDADATFDKEIVLDASTMTPFVTWGTNPGQGVPLGGSVPDPAQYDDPSDRIAAE
KACEYMGLEAGTPMRDIKVDTVFIGSCTNGRIEDLRAAAEIIKGRQVDKSTRLLVVPGSVRVRLQAQDEGLDVIFKEAGG
EWRGAGCSMCLGMNPDTLQPGERSASTSNRNFEGRQGKGGRTHLVSVPVAAATAIRGTLSSPADLEPVGSN

Sequences:

>Translated_471_residues
MGRTLAEKVWDEHVVRSTPGEPDLLYIDLHLIHEVTSPQAFDGLRLAGRTVRRPDLTLATEDHNVPTLDWDKPIADPVSK
TQVDTLRRNAAEFGVRLHPLGDVEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQ
AKPKTMAVTVEGSLPDGVTAKDLVLTLIAHTGTGGGQGYIVEYRGPAIEELSMEGRMTVCNMSIEWGAKAGLIAPDQTTF
DYIEGRPEAPKGADWDAAVAHWKTLVTDADATFDKEIVLDASTMTPFVTWGTNPGQGVPLGGSVPDPAQYDDPSDRIAAE
KACEYMGLEAGTPMRDIKVDTVFIGSCTNGRIEDLRAAAEIIKGRQVDKSTRLLVVPGSVRVRLQAQDEGLDVIFKEAGG
EWRGAGCSMCLGMNPDTLQPGERSASTSNRNFEGRQGKGGRTHLVSVPVAAATAIRGTLSSPADLEPVGSN
>Mature_470_residues
GRTLAEKVWDEHVVRSTPGEPDLLYIDLHLIHEVTSPQAFDGLRLAGRTVRRPDLTLATEDHNVPTLDWDKPIADPVSKT
QVDTLRRNAAEFGVRLHPLGDVEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQA
KPKTMAVTVEGSLPDGVTAKDLVLTLIAHTGTGGGQGYIVEYRGPAIEELSMEGRMTVCNMSIEWGAKAGLIAPDQTTFD
YIEGRPEAPKGADWDAAVAHWKTLVTDADATFDKEIVLDASTMTPFVTWGTNPGQGVPLGGSVPDPAQYDDPSDRIAAEK
ACEYMGLEAGTPMRDIKVDTVFIGSCTNGRIEDLRAAAEIIKGRQVDKSTRLLVVPGSVRVRLQAQDEGLDVIFKEAGGE
WRGAGCSMCLGMNPDTLQPGERSASTSNRNFEGRQGKGGRTHLVSVPVAAATAIRGTLSSPADLEPVGSN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=396, Percent_Identity=27.7777777777778, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI8659555, Length=460, Percent_Identity=25.8695652173913, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI41352693, Length=382, Percent_Identity=25.130890052356, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786259, Length=446, Percent_Identity=62.780269058296, Blast_Score=573, Evalue=1e-165,
Organism=Escherichia coli, GI1787531, Length=376, Percent_Identity=26.063829787234, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081781, Length=359, Percent_Identity=26.1838440111421, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI2367097, Length=129, Percent_Identity=35.6589147286822, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564738, Length=406, Percent_Identity=30.2955665024631, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25149337, Length=406, Percent_Identity=30.2955665024631, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=32.0754716981132, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=373, Percent_Identity=26.0053619302949, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321429, Length=451, Percent_Identity=59.6452328159645, Blast_Score=544, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6320440, Length=491, Percent_Identity=25.8655804480652, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=390, Percent_Identity=28.2051282051282, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=399, Percent_Identity=28.0701754385965, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161076999, Length=402, Percent_Identity=27.363184079602, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI281365315, Length=402, Percent_Identity=27.363184079602, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI17864292, Length=402, Percent_Identity=27.363184079602, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571643, Length=408, Percent_Identity=27.9411764705882, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=26.4550264550265, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI17137564, Length=381, Percent_Identity=25.7217847769029, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_NOCSJ (A1SLW5)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924487.1
- STRING:   A1SLW5
- GeneID:   4598170
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3298
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1SLW5
- BioCyc:   NSP35761:NOCA_3298-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50124; Mature: 49993

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTLAEKVWDEHVVRSTPGEPDLLYIDLHLIHEVTSPQAFDGLRLAGRTVRRPDLTLAT
CCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCCHHCCHHHCCCEECCCCEEEEE
EDHNVPTLDWDKPIADPVSKTQVDTLRRNAAEFGVRLHPLGDVEQGIVHVVGPQLGLTQP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCHHHCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQAKPKTMAVTVEGSLPDGVTA
CCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
KDLVLTLIAHTGTGGGQGYIVEYRGPAIEELSMEGRMTVCNMSIEWGAKAGLIAPDQTTF
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCH
DYIEGRPEAPKGADWDAAVAHWKTLVTDADATFDKEIVLDASTMTPFVTWGTNPGQGVPL
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCC
GGSVPDPAQYDDPSDRIAAEKACEYMGLEAGTPMRDIKVDTVFIGSCTNGRIEDLRAAAE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHH
IIKGRQVDKSTRLLVVPGSVRVRLQAQDEGLDVIFKEAGGEWRGAGCSMCLGMNPDTLQP
HHCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCC
GERSASTSNRNFEGRQGKGGRTHLVSVPVAAATAIRGTLSSPADLEPVGSN
CCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GRTLAEKVWDEHVVRSTPGEPDLLYIDLHLIHEVTSPQAFDGLRLAGRTVRRPDLTLAT
CCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEHHCCCCCHHCCHHHCCCEECCCCEEEEE
EDHNVPTLDWDKPIADPVSKTQVDTLRRNAAEFGVRLHPLGDVEQGIVHVVGPQLGLTQP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEECCCCHHHCEEEEECCCCCCCCC
GMTIVCGDSHTSTHGAFGAIAFGIGTSEVEHVLATQTLPQAKPKTMAVTVEGSLPDGVTA
CCEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCH
KDLVLTLIAHTGTGGGQGYIVEYRGPAIEELSMEGRMTVCNMSIEWGAKAGLIAPDQTTF
HHHHHHHHHCCCCCCCCEEEEEECCCCHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCH
DYIEGRPEAPKGADWDAAVAHWKTLVTDADATFDKEIVLDASTMTPFVTWGTNPGQGVPL
HHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEEECCCCCCCCCC
GGSVPDPAQYDDPSDRIAAEKACEYMGLEAGTPMRDIKVDTVFIGSCTNGRIEDLRAAAE
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHEEEEEEEEECCCCCHHHHHHHHHH
IIKGRQVDKSTRLLVVPGSVRVRLQAQDEGLDVIFKEAGGEWRGAGCSMCLGMNPDTLQP
HHCCCCCCCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCCCCCEEEEECCCCCCCCC
GERSASTSNRNFEGRQGKGGRTHLVSVPVAAATAIRGTLSSPADLEPVGSN
CCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA