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The map label for this gene is ogt [H]

Identifier: 119717524

GI number: 119717524

Start: 3506105

End: 3506584

Strand: Reverse

Name: ogt [H]

Synonym: Noca_3300

Alternate gene names: 119717524

Gene position: 3506584-3506105 (Counterclockwise)

Preceding gene: 119717525

Following gene: 119717522

Centisome position: 70.33

GC content: 71.88

Gene sequence:

>480_bases
ATGTGGACCGTGATGGACTCACCCGTCGGTGAGCTGCGGCTCGTCGAGCACAACGGCGCGATCACCGCGATCGAGTTCTC
GCCGTTCCGCGACAACGACGGGCGGCCGCGCGGCGACCGCGGCGACGACCACCCGCTCCTCGTCGAGGCCGCCCGGCAGC
TGCGTGCGTACTTCGACCGCGACCTCAAGGAGTTCGACCTGCCGCTCGCCCCTGCCGGCAGCGCGTTCCAGCAGCGGGTC
TGGCACGAGCTGCTCGGCGTCGGGTGGGGCGAGACCGCGTCGTACGGCGAGATCGCCCACCGCCTCGGCATGACCAACGC
GGCCTCGCGCGCCGTCGGCCTGGCGAACGGCCGCAACCCGATCCCGATCGTGATCCCGTGCCACCGGATCATCGGCGCCA
ACGGCACCCTGACCGGCTACGCCGGCGGCCTGCCGCGCAAGCAGCTGCTCCTCGAGCTCGAGCAGGATGCGCTGTTCTGA

Upstream 100 bases:

>100_bases
CGGGACCCGTCGACGGCGGCGGACCTCGCTCGGCGTTGGTCGCCCTGGCGCTCCTACGCGCAGGTGTACCTCTGGCAGAC
CCTCAGCAAGGAGAACTGAG

Downstream 100 bases:

>100_bases
GCGCAGGTCGAGCGCGACGGAGCAGTCGGGACAGCGATGGCCCGGACGACCGAGCCCGATCCGCTACTCCGCGGCGGCTC
GCCGTAGGGACTCGCTGAGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MWTVMDSPVGELRLVEHNGAITAIEFSPFRDNDGRPRGDRGDDHPLLVEAARQLRAYFDRDLKEFDLPLAPAGSAFQQRV
WHELLGVGWGETASYGEIAHRLGMTNAASRAVGLANGRNPIPIVIPCHRIIGANGTLTGYAGGLPRKQLLLELEQDALF

Sequences:

>Translated_159_residues
MWTVMDSPVGELRLVEHNGAITAIEFSPFRDNDGRPRGDRGDDHPLLVEAARQLRAYFDRDLKEFDLPLAPAGSAFQQRV
WHELLGVGWGETASYGEIAHRLGMTNAASRAVGLANGRNPIPIVIPCHRIIGANGTLTGYAGGLPRKQLLLELEQDALF
>Mature_159_residues
MWTVMDSPVGELRLVEHNGAITAIEFSPFRDNDGRPRGDRGDDHPLLVEAARQLRAYFDRDLKEFDLPLAPAGSAFQQRV
WHELLGVGWGETASYGEIAHRLGMTNAASRAVGLANGRNPIPIVIPCHRIIGANGTLTGYAGGLPRKQLLLELEQDALF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=34.3373493975904, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=53.3980582524272, Blast_Score=103, Evalue=5e-24,
Organism=Escherichia coli, GI1788542, Length=164, Percent_Identity=32.3170731707317, Blast_Score=84, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=40.7407407407407, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=46.4285714285714, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17439; Mature: 17439

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWTVMDSPVGELRLVEHNGAITAIEFSPFRDNDGRPRGDRGDDHPLLVEAARQLRAYFDR
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DLKEFDLPLAPAGSAFQQRVWHELLGVGWGETASYGEIAHRLGMTNAASRAVGLANGRNP
CHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHEECCCCCCC
IPIVIPCHRIIGANGTLTGYAGGLPRKQLLLELEQDALF
CEEEEECHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MWTVMDSPVGELRLVEHNGAITAIEFSPFRDNDGRPRGDRGDDHPLLVEAARQLRAYFDR
CCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
DLKEFDLPLAPAGSAFQQRVWHELLGVGWGETASYGEIAHRLGMTNAASRAVGLANGRNP
CHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHEECCCCCCC
IPIVIPCHRIIGANGTLTGYAGGLPRKQLLLELEQDALF
CEEEEECHHHHCCCCCEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]