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The map label for this gene is leuD

Identifier: 119717521

GI number: 119717521

Start: 3503181

End: 3503789

Strand: Reverse

Name: leuD

Synonym: Noca_3297

Alternate gene names: 119717521

Gene position: 3503789-3503181 (Counterclockwise)

Preceding gene: 119717522

Following gene: 119717520

Centisome position: 70.27

GC content: 69.62

Gene sequence:

>609_bases
ATGGACAAGTTCACCACCCACACCGGCGTCGGGATCCCGCTGCGGCGCAGCAACGTCGACACCGACCAGATCATCCCGGC
CGTCTACCTCAAGCGGGTCACCCGCACCGGCTTCGAGGACGGGCTGTTTGCGGCCTGGCGCAACGACCCGAGCTTCGTGC
TCAACAACGAGGGGTACGCCGGGGCCTCGGTCCTCGTGGCCGGGCCGGACTTCGGCACCGGTTCGTCGCGCGAGCACGCC
GTGTGGGCGCTGCAGAACTACGGCTTCAAGGTCGTGATCTCCCCGCGGTTCGCCGACATCTTCCGCGGCAACTCCGGCAA
GGCGGGGCTGCTGGCCGCGCAGGTCGACGAGTCGGTCGTCCAGAAGATCTGGGACCTGCTCGACGAGCACCCTGGCACGG
CCGTGACCGTCGACCTGGAGTCGCGGACCGTCCGGGCGGGCGAGGGGGTCGACGCGATCGAGGCCTCCTTCGACATCGAC
GACTACACCCGCTGGCGGCTGCTCGAAGGGCTCGACGACATCGGCATCACGCTCGGGCACGCCGACGCGATCGCGTCGTA
CGAGGCGACCCGACCGAGCTGGAGGCCGGCGACGATCCACGCGCACTGA

Upstream 100 bases:

>100_bases
GGCCGCACCCACCTCGTGTCGGTGCCGGTCGCCGCCGCGACCGCGATCCGCGGCACCCTGTCCTCGCCCGCCGACCTCGA
GCCCGTTGGGAGCAACTGAG

Downstream 100 bases:

>100_bases
CGGCGCCACGAGAACATTCGAGAAGGGCGAATCCCCAAGCAGGCTTGGGGATTCGCCCTTTCCATAACCGCAATCCCGCT
CTGCTGACCGGATTCCGGCG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 202; Mature: 202

Protein sequence:

>202_residues
MDKFTTHTGVGIPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAAWRNDPSFVLNNEGYAGASVLVAGPDFGTGSSREHA
VWALQNYGFKVVISPRFADIFRGNSGKAGLLAAQVDESVVQKIWDLLDEHPGTAVTVDLESRTVRAGEGVDAIEASFDID
DYTRWRLLEGLDDIGITLGHADAIASYEATRPSWRPATIHAH

Sequences:

>Translated_202_residues
MDKFTTHTGVGIPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAAWRNDPSFVLNNEGYAGASVLVAGPDFGTGSSREHA
VWALQNYGFKVVISPRFADIFRGNSGKAGLLAAQVDESVVQKIWDLLDEHPGTAVTVDLESRTVRAGEGVDAIEASFDID
DYTRWRLLEGLDDIGITLGHADAIASYEATRPSWRPATIHAH
>Mature_202_residues
MDKFTTHTGVGIPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAAWRNDPSFVLNNEGYAGASVLVAGPDFGTGSSREHA
VWALQNYGFKVVISPRFADIFRGNSGKAGLLAAQVDESVVQKIWDLLDEHPGTAVTVDLESRTVRAGEGVDAIEASFDID
DYTRWRLLEGLDDIGITLGHADAIASYEATRPSWRPATIHAH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=201, Percent_Identity=49.2537313432836, Blast_Score=190, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321429, Length=202, Percent_Identity=40.5940594059406, Blast_Score=146, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_NOCSJ (A1SLW4)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924486.1
- STRING:   A1SLW4
- GeneID:   4598169
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3297
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   A1SLW4
- BioCyc:   NSP35761:NOCA_3297-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22057; Mature: 22057

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKFTTHTGVGIPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAAWRNDPSFVLNNEGYA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCC
GASVLVAGPDFGTGSSREHAVWALQNYGFKVVISPRFADIFRGNSGKAGLLAAQVDESVV
CCEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCEEEEHHHHHHHH
QKIWDLLDEHPGTAVTVDLESRTVRAGEGVDAIEASFDIDDYTRWRLLEGLDDIGITLGH
HHHHHHHHCCCCCEEEEEECCCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEECC
ADAIASYEATRPSWRPATIHAH
HHHHHHCCCCCCCCCCEEEECC
>Mature Secondary Structure
MDKFTTHTGVGIPLRRSNVDTDQIIPAVYLKRVTRTGFEDGLFAAWRNDPSFVLNNEGYA
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCC
GASVLVAGPDFGTGSSREHAVWALQNYGFKVVISPRFADIFRGNSGKAGLLAAQVDESVV
CCEEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCHHHHHCCCCCCCEEEEHHHHHHHH
QKIWDLLDEHPGTAVTVDLESRTVRAGEGVDAIEASFDIDDYTRWRLLEGLDDIGITLGH
HHHHHHHHCCCCCEEEEEECCCEEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEECC
ADAIASYEATRPSWRPATIHAH
HHHHHHCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA