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The map label for this gene is pdxT

Identifier: 119716609

GI number: 119716609

Start: 2538014

End: 2538619

Strand: Direct

Name: pdxT

Synonym: Noca_2382

Alternate gene names: 119716609

Gene position: 2538014-2538619 (Clockwise)

Preceding gene: 119716608

Following gene: 119716610

Centisome position: 50.9

GC content: 70.63

Gene sequence:

>606_bases
GTGACGACCGCCCCCACGATCGGTGTCTTCGCCCTCCAGGGCGACGTCCGCGAGCACCTCGGGATGCTCACCGGCCTCGG
TGTCGAGGCGATCGCCGTACGCCGTCCCGCCGAGCTGGACGTGTGCGCGGGCCTGGTGATCCCAGGTGGCGAGTCGACCA
CGATGGCCAAGCTGGCTCGCACCTTCGACCTGTTCGAGCCGATCCGGCAGCGGATCAAGGAGGGCATGCCCGCCTTCGGG
ACGTGCGCCGGCATGATCATGCTCGCGGACCGGATCGAGGACGGCACCCGCGACCAGGAGACGCTCGGCGGCCTGGACAT
CACGGTGCGGCGCAACGCGTTCGGGCGCCAGGTCGAGTCGTTCGAGGGTGAGATCGACGTCGTCGGCCTCGACGCGCCCC
TGCACGCGGTCTTCATCCGCGCGCCCTGGGTGGAGGCCGTGGGCGACTCGGTCGAGGTGCTCGCGCGTGTCGAGCAGGGC
GAGGCCGCGGGTAGGATCGTCGCGGTCCGTCAGGGTTCGCTGATGGCGACGTCGTTCCACCCCGAGGTGGGTGGCGACTC
CCGGGTCCACCGGCTGTTCGTGGACCTGGTCACCGAGGAGAGATAG

Upstream 100 bases:

>100_bases
CCGACGTGGTCGCGAAGGTCTCCCGCGGTCTCGGTGAGGCCATGGTCGGCATCAACGTCGAGGAGCTTCCCCAGCCGCAC
CGGCTCGCCGAGCGCGGCTG

Downstream 100 bases:

>100_bases
GGATACGCCATGTCTGGCCACTCCAAGTGGGCGACCACCAAGCACAAGAAGGCGGCGATCGACGCCAAGCGCGGCAAGCT
GTTCGCCAAGCTGATCAAGA

Product: SNO glutamine amidotransferase

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MTTAPTIGVFALQGDVREHLGMLTGLGVEAIAVRRPAELDVCAGLVIPGGESTTMAKLARTFDLFEPIRQRIKEGMPAFG
TCAGMIMLADRIEDGTRDQETLGGLDITVRRNAFGRQVESFEGEIDVVGLDAPLHAVFIRAPWVEAVGDSVEVLARVEQG
EAAGRIVAVRQGSLMATSFHPEVGGDSRVHRLFVDLVTEER

Sequences:

>Translated_201_residues
MTTAPTIGVFALQGDVREHLGMLTGLGVEAIAVRRPAELDVCAGLVIPGGESTTMAKLARTFDLFEPIRQRIKEGMPAFG
TCAGMIMLADRIEDGTRDQETLGGLDITVRRNAFGRQVESFEGEIDVVGLDAPLHAVFIRAPWVEAVGDSVEVLARVEQG
EAAGRIVAVRQGSLMATSFHPEVGGDSRVHRLFVDLVTEER
>Mature_200_residues
TTAPTIGVFALQGDVREHLGMLTGLGVEAIAVRRPAELDVCAGLVIPGGESTTMAKLARTFDLFEPIRQRIKEGMPAFGT
CAGMIMLADRIEDGTRDQETLGGLDITVRRNAFGRQVESFEGEIDVVGLDAPLHAVFIRAPWVEAVGDSVEVLARVEQGE
AAGRIVAVRQGSLMATSFHPEVGGDSRVHRLFVDLVTEER

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=36.7647058823529, Blast_Score=120, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6323995, Length=210, Percent_Identity=35.2380952380952, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321048, Length=210, Percent_Identity=34.7619047619048, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_NOCSJ (A1SJA2)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_923574.1
- ProteinModelPortal:   A1SJA2
- SMR:   A1SJA2
- STRING:   A1SJA2
- GeneID:   4599482
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_2382
- eggNOG:   COG0311
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- PhylomeDB:   A1SJA2
- BioCyc:   NSP35761:NOCA_2382-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21626; Mature: 21495

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 82-82 ACT_SITE 180-180 ACT_SITE 182-182 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAPTIGVFALQGDVREHLGMLTGLGVEAIAVRRPAELDVCAGLVIPGGESTTMAKLAR
CCCCCCEEEEEECCHHHHHHHHHHCCCHHEEEECCCCCCHHEEEEEECCCCCHHHHHHHH
TFDLFEPIRQRIKEGMPAFGTCAGMIMLADRIEDGTRDQETLGGLDITVRRNAFGRQVES
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCHHHHHHHH
FEGEIDVVGLDAPLHAVFIRAPWVEAVGDSVEVLARVEQGEAAGRIVAVRQGSLMATSFH
CCCCEEEEEECCCEEEEEEECCHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
PEVGGDSRVHRLFVDLVTEER
CCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTAPTIGVFALQGDVREHLGMLTGLGVEAIAVRRPAELDVCAGLVIPGGESTTMAKLAR
CCCCCEEEEEECCHHHHHHHHHHCCCHHEEEECCCCCCHHEEEEEECCCCCHHHHHHHH
TFDLFEPIRQRIKEGMPAFGTCAGMIMLADRIEDGTRDQETLGGLDITVRRNAFGRQVES
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCEEEEEECCHHHHHHHH
FEGEIDVVGLDAPLHAVFIRAPWVEAVGDSVEVLARVEQGEAAGRIVAVRQGSLMATSFH
CCCCEEEEEECCCEEEEEEECCHHHHHCCHHHHHHHHHCCCCCCEEEEEECCCEEEEECC
PEVGGDSRVHRLFVDLVTEER
CCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA