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The map label for this gene is pncA [H]

Identifier: 119715537

GI number: 119715537

Start: 1375042

End: 1375626

Strand: Reverse

Name: pncA [H]

Synonym: Noca_1301

Alternate gene names: 119715537

Gene position: 1375626-1375042 (Counterclockwise)

Preceding gene: 119715538

Following gene: 119715536

Centisome position: 27.59

GC content: 72.48

Gene sequence:

>585_bases
ATGAGCGAGTCGAAGCGTGCCCTCGTCGTGACCGACGTGCAGAACGACTTCTGCGAAGGCGGCTCACTGCCGGTGGCGGG
CGGGGCGCGGGTGGCGGCAGACATCGCCGAGGTCCTGCACCTGTGGAGCGTGCAGGGGCCCGGGGCGCCCGACTACGCGC
ACGTAGTGGCCACCAAGGACCACCACATAGATCCGGGCGAGCACTGGTCCCGCGAGCCGGACTACCTCGACTCCTGGCCG
GTGCACTGCCGGGTGGGGACCGACGGCGCCTCCTTCCACCCGAACCTCGACCCCCAGCCGTTCGACGCCATCTTCTTCAA
GGGCGAGCACCAGGCGGCGTACTCCGGGTTCGAGGGCCGCACCGTCGAGGGGGTGGGGCTGGCCGAGTGGCTGCGGTCGC
GCGGCGTGACCGAGGTGGACGTGTGCGGCATCGCGACCGACTACTGCGTGCGCAAGACCGCCCTGGACGCCGTGCGGGAG
GGCTTCACGACGCGGCTCCTCGCCGACCTGTGCGCGGGCGTGGCCCCGGACACCTCGGCCGCGGCGCTCGAGGAGATGCG
GGCCGCCGGGGTGAGCGTTGCCTGA

Upstream 100 bases:

>100_bases
GTCATGGGCGCGATCGCCGTCGCCCTCGACCCTCTCGAGGCCCCCCGGTCGGGCCGCCAGCACTCCCGGTCGCACGGCGC
CGGGTGACAGACTCCAGGGC

Downstream 100 bases:

>100_bases
CCTCGAGGTCGACACCACCGACGGGGTGCTGCGGCTGAGGTTCAACCGCCCGGAGCTGCTCAACGCGCTCACCGGCGAGA
TGGCCGACGAGCTCGCCGGC

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MSESKRALVVTDVQNDFCEGGSLPVAGGARVAADIAEVLHLWSVQGPGAPDYAHVVATKDHHIDPGEHWSREPDYLDSWP
VHCRVGTDGASFHPNLDPQPFDAIFFKGEHQAAYSGFEGRTVEGVGLAEWLRSRGVTEVDVCGIATDYCVRKTALDAVRE
GFTTRLLADLCAGVAPDTSAAALEEMRAAGVSVA

Sequences:

>Translated_194_residues
MSESKRALVVTDVQNDFCEGGSLPVAGGARVAADIAEVLHLWSVQGPGAPDYAHVVATKDHHIDPGEHWSREPDYLDSWP
VHCRVGTDGASFHPNLDPQPFDAIFFKGEHQAAYSGFEGRTVEGVGLAEWLRSRGVTEVDVCGIATDYCVRKTALDAVRE
GFTTRLLADLCAGVAPDTSAAALEEMRAAGVSVA
>Mature_193_residues
SESKRALVVTDVQNDFCEGGSLPVAGGARVAADIAEVLHLWSVQGPGAPDYAHVVATKDHHIDPGEHWSREPDYLDSWPV
HCRVGTDGASFHPNLDPQPFDAIFFKGEHQAAYSGFEGRTVEGVGLAEWLRSRGVTEVDVCGIATDYCVRKTALDAVREG
FTTRLLADLCAGVAPDTSAAALEEMRAAGVSVA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=208, Percent_Identity=38.4615384615385, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 20690; Mature: 20559

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESKRALVVTDVQNDFCEGGSLPVAGGARVAADIAEVLHLWSVQGPGAPDYAHVVATKD
CCCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
HHIDPGEHWSREPDYLDSWPVHCRVGTDGASFHPNLDPQPFDAIFFKGEHQAAYSGFEGR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCC
TVEGVGLAEWLRSRGVTEVDVCGIATDYCVRKTALDAVREGFTTRLLADLCAGVAPDTSA
EECCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AALEEMRAAGVSVA
HHHHHHHHCCCCCC
>Mature Secondary Structure 
SESKRALVVTDVQNDFCEGGSLPVAGGARVAADIAEVLHLWSVQGPGAPDYAHVVATKD
CCCCCEEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
HHIDPGEHWSREPDYLDSWPVHCRVGTDGASFHPNLDPQPFDAIFFKGEHQAAYSGFEGR
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHHCCCCCC
TVEGVGLAEWLRSRGVTEVDVCGIATDYCVRKTALDAVREGFTTRLLADLCAGVAPDTSA
EECCCCHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
AALEEMRAAGVSVA
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]