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The map label for this gene is ppsA [H]

Identifier: 119715421

GI number: 119715421

Start: 1259324

End: 1260151

Strand: Direct

Name: ppsA [H]

Synonym: Noca_1185

Alternate gene names: 119715421

Gene position: 1259324-1260151 (Clockwise)

Preceding gene: 119715420

Following gene: 119715422

Centisome position: 25.26

GC content: 69.93

Gene sequence:

>828_bases
ATGCCCGAGACCCCCCTCGTCCTCCCCTTCACCGACGCGCGATGCCGCGAGGTCGCGTTGTCGGGGGGCAAGGGCGCGAG
CCTCGCCGCCATGACCCAGAACGGGCTCCCGGTGCCGCCCGGCTTCGTGATCACGTCGACCGCGTTCGGGGAGGCGGTGG
ATGCGGGGAGGCTCTTGGGCTGCCTCGAGGTCCGAGACCTGGAGGGCGCCCGTGCCGTCGTGGCGGAGACCGAGCCGCCC
GTCGAGCTGGTGAGGGCCGCCTACGACGAGCTACCGCGAGGCACCTCCGTGGCGGTGCGGTCCTCGGCCTGCGCCGAGGA
CGGGAACGACGCGTCGTACGCGGGCCAGCAGGAGACCTACCTGTTCGTCGAGTCGTTGGACGACGTGCTGGAGAAGGTGG
TGGACTGCTGGCTCTCCTTCTTCTCCGATCGTGCGCTGTTCTATCGCGAGCACAAGGGAGACCTGCAGGACATCTCCATG
GCCGTCGTCGTCCAGCAGATGGTCGACGCGGAGAAGGCGGGTGTCATGTTCACCGTCGACCCGGTGAACCACCGCCGGGA
CCGCATCGTCGTCGAGGCCGCCCGCGGCGTCGGGGAGCACGTCGTCTCCGGCGAGGTCACCCCCGACTACTACACGCTGG
ACCGGCAGGGAACGCTGAAGAAGGCGCGGGTCGTCGACGAACAGGTCCTCTCGGAGGCCGAGCTGGCCGAGCTCGCGCGG
CTGGGGCTCCACCTCGCGGAGCTGAACGGCTGCCCCCAGGACATCGAGTGGGCCTACGACTCCACCGGCCTCTACATGCT
GCAGTCGCGGCCCGTCACGACGCTCTAG

Upstream 100 bases:

>100_bases
CCGACCAAGGTGAGAGCGCGGCGGACCGGGCTGCCCATGCGCGAGGTCAGCTGTGAGCACGGCGGTCCGACCGGAAGCAT
CCATTCGGAAGGACGCGTCC

Downstream 100 bases:

>100_bases
CGACCACCTGATGTCCATGGGCGGAGCCGGGGAGGCCGGCCAGTGAGGTACGTGACCTACGAGTCTCCGGAAGGCCGCGG
TCGCCGGGTCGGGTACCTCG

Product: pyruvate, water dikinase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase; Mja pepA intein [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MPETPLVLPFTDARCREVALSGGKGASLAAMTQNGLPVPPGFVITSTAFGEAVDAGRLLGCLEVRDLEGARAVVAETEPP
VELVRAAYDELPRGTSVAVRSSACAEDGNDASYAGQQETYLFVESLDDVLEKVVDCWLSFFSDRALFYREHKGDLQDISM
AVVVQQMVDAEKAGVMFTVDPVNHRRDRIVVEAARGVGEHVVSGEVTPDYYTLDRQGTLKKARVVDEQVLSEAELAELAR
LGLHLAELNGCPQDIEWAYDSTGLYMLQSRPVTTL

Sequences:

>Translated_275_residues
MPETPLVLPFTDARCREVALSGGKGASLAAMTQNGLPVPPGFVITSTAFGEAVDAGRLLGCLEVRDLEGARAVVAETEPP
VELVRAAYDELPRGTSVAVRSSACAEDGNDASYAGQQETYLFVESLDDVLEKVVDCWLSFFSDRALFYREHKGDLQDISM
AVVVQQMVDAEKAGVMFTVDPVNHRRDRIVVEAARGVGEHVVSGEVTPDYYTLDRQGTLKKARVVDEQVLSEAELAELAR
LGLHLAELNGCPQDIEWAYDSTGLYMLQSRPVTTL
>Mature_274_residues
PETPLVLPFTDARCREVALSGGKGASLAAMTQNGLPVPPGFVITSTAFGEAVDAGRLLGCLEVRDLEGARAVVAETEPPV
ELVRAAYDELPRGTSVAVRSSACAEDGNDASYAGQQETYLFVESLDDVLEKVVDCWLSFFSDRALFYREHKGDLQDISMA
VVVQQMVDAEKAGVMFTVDPVNHRRDRIVVEAARGVGEHVVSGEVTPDYYTLDRQGTLKKARVVDEQVLSEAELAELARL
GLHLAELNGCPQDIEWAYDSTGLYMLQSRPVTTL

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DOD-type homing endonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=333, Percent_Identity=35.1351351351351, Blast_Score=139, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17564524, Length=285, Percent_Identity=36.8421052631579, Blast_Score=159, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR003587
- InterPro:   IPR004042
- InterPro:   IPR006141
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR006319
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 29865; Mature: 29734

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPETPLVLPFTDARCREVALSGGKGASLAAMTQNGLPVPPGFVITSTAFGEAVDAGRLLG
CCCCCEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
CLEVRDLEGARAVVAETEPPVELVRAAYDELPRGTSVAVRSSACAEDGNDASYAGQQETY
HEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECHHCCCCCCCCCCCCCCHHH
LFVESLDDVLEKVVDCWLSFFSDRALFYREHKGDLQDISMAVVVQQMVDAEKAGVMFTVD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEC
PVNHRRDRIVVEAARGVGEHVVSGEVTPDYYTLDRQGTLKKARVVDEQVLSEAELAELAR
CCCCCHHEEEEEHHHCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LGLHLAELNGCPQDIEWAYDSTGLYMLQSRPVTTL
HHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCC
>Mature Secondary Structure 
PETPLVLPFTDARCREVALSGGKGASLAAMTQNGLPVPPGFVITSTAFGEAVDAGRLLG
CCCCEEEECCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHH
CLEVRDLEGARAVVAETEPPVELVRAAYDELPRGTSVAVRSSACAEDGNDASYAGQQETY
HEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECHHCCCCCCCCCCCCCCHHH
LFVESLDDVLEKVVDCWLSFFSDRALFYREHKGDLQDISMAVVVQQMVDAEKAGVMFTVD
HHHHHHHHHHHHHHHHHHHHHCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHHCCEEEEEC
PVNHRRDRIVVEAARGVGEHVVSGEVTPDYYTLDRQGTLKKARVVDEQVLSEAELAELAR
CCCCCHHEEEEEHHHCCHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
LGLHLAELNGCPQDIEWAYDSTGLYMLQSRPVTTL
HHHHHHHHCCCCCCCCEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]