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The map label for this gene is yvkC [H]

Identifier: 119715420

GI number: 119715420

Start: 1257501

End: 1259279

Strand: Direct

Name: yvkC [H]

Synonym: Noca_1184

Alternate gene names: 119715420

Gene position: 1257501-1259279 (Clockwise)

Preceding gene: 119715419

Following gene: 119715421

Centisome position: 25.22

GC content: 66.39

Gene sequence:

>1779_bases
ATGACCCAGACCGTTGCGACCTTCTTCGGAGACGAGGAGTTCCCTGTCCGCTGGGAGGACGGCCAACAGGACCTCCTGTG
GGTCCACGACGACCTGCACATCCCCAACCCGGTCTCGCCGATGTACGCCGACATCGGCGGATGGTGGCTCAAGTGCGACT
ACATGTTCCGTCGGTTCGGGACACCCTTCGCCTCGGACTGGATCTCCAAGGTCGTCAACGGGTACGTCTACACCGCGGCG
ATCCCGGCGCAGCCCGGACTGTCGGCCGAGACGACCGAGTACGGCGCCCGCTACGTGCCTCGCGTCCCGGACAACGACGA
GTACGCCGCGGAGATCGGTGGCTACCTCGGCTGGACGTTGCCCTACTACGCAGAGAACTTCCTGCACTGGTGGCGGGATC
GTCTCGTGCCCGAGATGACGCGCAACTTCGAGCGCTTCGACGCCTACGACTACGAGTCCGCCAGCCTGGTCGAGCTCGCG
ATCCTCCTCGAGGACGCGATCGACATGCACGACCGCCACTGGCAGATCCACTGGGTGCTCAACTTCGCCCAGTTCTCGTC
CACCACGAACCTCAACGCCATCATCGCCGAGGTGAAGGGTGAGGGCGACCACTCCGACCTGATGGGTCGCCTGCAGAGCT
CGCTGGAGAACCGCAACTGGGACTCCATCGAGGAGCTGTGGAAGATCAAGGAGCAGATCAGGAAGGATGGCGGACCGGTC
GCCGATGCCTTCACGAGGGACACCGCGTCCGAGATCCGGGTGGCGCTGGAATCGACGCCCGAGGGCCAGGCCTTCCTGGC
GAAGGAGATCGAGTCCTACCAGGCCGTCTTCGGGTACAAGTCGATGTACGCCCACGAGTTCTCTTTCAAGACCTGGCGGG
AGGACCCCGCTCCGGTCCTGGAGTCGATCCGCAGCTACCTCGAGGCCGACTACGACTACCCGGCCGAGATCGCTGCGGTC
GGCAAGGACCTCGAGGCGGCCAAGGCGGAGGCGGTCGACGGCGTCCAGGGCGAGGACCTCGACCGCCTCCGGAGCGCGCT
CGACCTGAGCCTCCGGATGAACCCGCTGACACCCGACCACCACTTCTACATCGACCAAGGAACCAACGCCCGGGTCCGGC
TGGTGCTGATCGCGATCGGCGAGATGCTGGTCGACCTCGGCAAGCTCGCCGACCCCGAGGACGTCATGTACCTGCGCTAC
AACGAGCTGCGCACCCTCATGGCCGGCAGCAACGGGTTCGACGCCGAGGCACTGGTCGGTGACCGCCGTGACGAACGTGA
GACCGCCTACGAGCTGCGCCCGCGCGACTGGTTGGGGACCGCGACCGAGGAGAACCTGGCGTTCCCGTACCTGGCGCTGT
GGGCGTTCCCGGACAAGGTCTACCGCAAGCCGTCGGAGGTCGAGGGCGAGATCCAGGGCCTGGGAGCCTCGAAGGGAGTC
ATCGAGGGGACGGCCCGCGTCGTCCTCTCGCCCGAGCAGTTCAGCCAGGTGGAGCAGGGCGAGATCATCATCTGCCGGAT
GACCAGCCCGTCCTGGGTCGTTCTGTTCACGAAGATCGGGGGCCTCGTCACCGATGCCGGTGGCATGGCGTCGCACCCGG
CGGTGGTCTCTCGTGAGTTCGGCATCCCGGCTGTCGTGGGGACCTCGGACGCGACCCGCAGGATCAAGACCGGTGACAAG
ATCCGCGTGAACGGGACGACCGGCATGGTCCAGGTGCTCTCGGCCGACCAAGGTGAGAGCGCGGCGGACCGGGCTGCCCA
TGCGCGAGGTCAGCTGTGA

Upstream 100 bases:

>100_bases
GTCCGAGCCTTGACGAGTGACCCACGTCACTTTAGATTATCGATAATCCATTGCAGTGCCGTAGCCACGAAGCTGACGCC
ACCCACCAGGAGGGGCCCCG

Downstream 100 bases:

>100_bases
GCACGGCGGTCCGACCGGAAGCATCCATTCGGAAGGACGCGTCCATGCCCGAGACCCCCCTCGTCCTCCCCTTCACCGAC
GCGCGATGCCGCGAGGTCGC

Product: PEP-utilising enzyme, mobile region

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MTQTVATFFGDEEFPVRWEDGQQDLLWVHDDLHIPNPVSPMYADIGGWWLKCDYMFRRFGTPFASDWISKVVNGYVYTAA
IPAQPGLSAETTEYGARYVPRVPDNDEYAAEIGGYLGWTLPYYAENFLHWWRDRLVPEMTRNFERFDAYDYESASLVELA
ILLEDAIDMHDRHWQIHWVLNFAQFSSTTNLNAIIAEVKGEGDHSDLMGRLQSSLENRNWDSIEELWKIKEQIRKDGGPV
ADAFTRDTASEIRVALESTPEGQAFLAKEIESYQAVFGYKSMYAHEFSFKTWREDPAPVLESIRSYLEADYDYPAEIAAV
GKDLEAAKAEAVDGVQGEDLDRLRSALDLSLRMNPLTPDHHFYIDQGTNARVRLVLIAIGEMLVDLGKLADPEDVMYLRY
NELRTLMAGSNGFDAEALVGDRRDERETAYELRPRDWLGTATEENLAFPYLALWAFPDKVYRKPSEVEGEIQGLGASKGV
IEGTARVVLSPEQFSQVEQGEIIICRMTSPSWVVLFTKIGGLVTDAGGMASHPAVVSREFGIPAVVGTSDATRRIKTGDK
IRVNGTTGMVQVLSADQGESAADRAAHARGQL

Sequences:

>Translated_592_residues
MTQTVATFFGDEEFPVRWEDGQQDLLWVHDDLHIPNPVSPMYADIGGWWLKCDYMFRRFGTPFASDWISKVVNGYVYTAA
IPAQPGLSAETTEYGARYVPRVPDNDEYAAEIGGYLGWTLPYYAENFLHWWRDRLVPEMTRNFERFDAYDYESASLVELA
ILLEDAIDMHDRHWQIHWVLNFAQFSSTTNLNAIIAEVKGEGDHSDLMGRLQSSLENRNWDSIEELWKIKEQIRKDGGPV
ADAFTRDTASEIRVALESTPEGQAFLAKEIESYQAVFGYKSMYAHEFSFKTWREDPAPVLESIRSYLEADYDYPAEIAAV
GKDLEAAKAEAVDGVQGEDLDRLRSALDLSLRMNPLTPDHHFYIDQGTNARVRLVLIAIGEMLVDLGKLADPEDVMYLRY
NELRTLMAGSNGFDAEALVGDRRDERETAYELRPRDWLGTATEENLAFPYLALWAFPDKVYRKPSEVEGEIQGLGASKGV
IEGTARVVLSPEQFSQVEQGEIIICRMTSPSWVVLFTKIGGLVTDAGGMASHPAVVSREFGIPAVVGTSDATRRIKTGDK
IRVNGTTGMVQVLSADQGESAADRAAHARGQL
>Mature_591_residues
TQTVATFFGDEEFPVRWEDGQQDLLWVHDDLHIPNPVSPMYADIGGWWLKCDYMFRRFGTPFASDWISKVVNGYVYTAAI
PAQPGLSAETTEYGARYVPRVPDNDEYAAEIGGYLGWTLPYYAENFLHWWRDRLVPEMTRNFERFDAYDYESASLVELAI
LLEDAIDMHDRHWQIHWVLNFAQFSSTTNLNAIIAEVKGEGDHSDLMGRLQSSLENRNWDSIEELWKIKEQIRKDGGPVA
DAFTRDTASEIRVALESTPEGQAFLAKEIESYQAVFGYKSMYAHEFSFKTWREDPAPVLESIRSYLEADYDYPAEIAAVG
KDLEAAKAEAVDGVQGEDLDRLRSALDLSLRMNPLTPDHHFYIDQGTNARVRLVLIAIGEMLVDLGKLADPEDVMYLRYN
ELRTLMAGSNGFDAEALVGDRRDERETAYELRPRDWLGTATEENLAFPYLALWAFPDKVYRKPSEVEGEIQGLGASKGVI
EGTARVVLSPEQFSQVEQGEIIICRMTSPSWVVLFTKIGGLVTDAGGMASHPAVVSREFGIPAVVGTSDATRRIKTGDKI
RVNGTTGMVQVLSADQGESAADRAAHARGQL

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=91, Percent_Identity=38.4615384615385, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17564524, Length=336, Percent_Identity=24.1071428571429, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 66490; Mature: 66359

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTVATFFGDEEFPVRWEDGQQDLLWVHDDLHIPNPVSPMYADIGGWWLKCDYMFRRFG
CCCEEEECCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEHHHHHHHHC
TPFASDWISKVVNGYVYTAAIPAQPGLSAETTEYGARYVPRVPDNDEYAAEIGGYLGWTL
CCHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCEECCCCCCCCHHHHHHCCHHCCCH
PYYAENFLHWWRDRLVPEMTRNFERFDAYDYESASLVELAILLEDAIDMHDRHWQIHWVL
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
NFAQFSSTTNLNAIIAEVKGEGDHSDLMGRLQSSLENRNWDSIEELWKIKEQIRKDGGPV
EEHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
ADAFTRDTASEIRVALESTPEGQAFLAKEIESYQAVFGYKSMYAHEFSFKTWREDPAPVL
HHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ESIRSYLEADYDYPAEIAAVGKDLEAAKAEAVDGVQGEDLDRLRSALDLSLRMNPLTPDH
HHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHEEEEEECCCCCCC
HFYIDQGTNARVRLVLIAIGEMLVDLGKLADPEDVMYLRYNELRTLMAGSNGFDAEALVG
EEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHHC
DRRDERETAYELRPRDWLGTATEENLAFPYLALWAFPDKVYRKPSEVEGEIQGLGASKGV
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCCHHCCCCCCCCE
IEGTARVVLSPEQFSQVEQGEIIICRMTSPSWVVLFTKIGGLVTDAGGMASHPAVVSREF
ECCCEEEEECHHHHHHCCCCCEEEEEECCCCEEEEEEHHCCEEECCCCCCCCCCHHHHCC
GIPAVVGTSDATRRIKTGDKIRVNGTTGMVQVLSADQGESAADRAAHARGQL
CCCEEECCCHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
TQTVATFFGDEEFPVRWEDGQQDLLWVHDDLHIPNPVSPMYADIGGWWLKCDYMFRRFG
CCEEEECCCCCCCCEEECCCCCCEEEEECCCCCCCCCCHHHHCCCCEEEEHHHHHHHHC
TPFASDWISKVVNGYVYTAAIPAQPGLSAETTEYGARYVPRVPDNDEYAAEIGGYLGWTL
CCHHHHHHHHHHCCEEEEEEECCCCCCCCCCHHHCCEECCCCCCCCHHHHHHCCHHCCCH
PYYAENFLHWWRDRLVPEMTRNFERFDAYDYESASLVELAILLEDAIDMHDRHWQIHWVL
HHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
NFAQFSSTTNLNAIIAEVKGEGDHSDLMGRLQSSLENRNWDSIEELWKIKEQIRKDGGPV
EEHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
ADAFTRDTASEIRVALESTPEGQAFLAKEIESYQAVFGYKSMYAHEFSFKTWREDPAPVL
HHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
ESIRSYLEADYDYPAEIAAVGKDLEAAKAEAVDGVQGEDLDRLRSALDLSLRMNPLTPDH
HHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHEEEEEECCCCCCC
HFYIDQGTNARVRLVLIAIGEMLVDLGKLADPEDVMYLRYNELRTLMAGSNGFDAEALVG
EEEEECCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCCHHHHC
DRRDERETAYELRPRDWLGTATEENLAFPYLALWAFPDKVYRKPSEVEGEIQGLGASKGV
CCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCCCCHHCCCCCCCCE
IEGTARVVLSPEQFSQVEQGEIIICRMTSPSWVVLFTKIGGLVTDAGGMASHPAVVSREF
ECCCEEEEECHHHHHHCCCCCEEEEEECCCCEEEEEEHHCCEEECCCCCCCCCCHHHHCC
GIPAVVGTSDATRRIKTGDKIRVNGTTGMVQVLSADQGESAADRAAHARGQL
CCCEEECCCHHHHHHCCCCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]