BacMap

Definition	Nocardioides sp. JS614 chromosome, complete genome.
Accession	NC_008699
Length	4,985,871

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The map label for this gene is braF [H]

Identifier: 119715417

GI number: 119715417

Start: 1254437

End: 1255249

Strand: Direct

Name: braF [H]

Synonym: Noca_1181

Alternate gene names: 119715417

Gene position: 1254437-1255249 (Clockwise)

Preceding gene: 119715416

Following gene: 119715418

Centisome position: 25.16

GC content: 66.42

Gene sequence:

>813_bases
GTGCCTGAATCCAGCACGACCTTCCGCACCGAGCTGCCGACCCGGACCGCCCCGCGCCCGCGGCTCACCCTCACCGACGT
CTCGAAGAGCTTCGCCGGCCGCACGGTGGTCGATGTCGAGGACCTCAGCCTCGGCGACCATCCCATCGAAGGACTCATCG
GCCCGAACGGTGCGGGCAAGACGACCCTGATGCGCCTGATCATGGGGTCTCTCAGGGCTGACACCGGGCAGATCCGTCTC
GAGCGGACGGGGCATCACGACGTCGTGCTCTCGCAGCTGCGCGCACACAAGATCGCTCAGTGCGGAGTCGTGAAGACCAA
CCAGGTGATCACTGACTTCGAGGGGCTGACGATCCTCGACTCGCTTCTGCTCTCGGTCACTCCCGAACGTCGTGAGTTCC
CCCTTCACCTGAAGGGGGAACGATCGACCTACGACGAGCACCGGGCCGAGATCGAGCGCCAGCTCGATCACTTCGGGCTG
GGGAACACCCGCCGGCTGGCGCTCTCGGCGGGCGAGAAGAAGCTCGTCGACATCATCCGATGCCTGCTGCTGAAGCCTGC
CGTCCTGCTCCTGGACGAGCCGACGGCGGGGCTTCCGGCAGAGGTGACCCAGAGGGTCATGGACGCCGTGGGCGGCCTGG
CCGCCGACGGCACCACCGTCGTCATCATCGAGCACGACCTCGACGTGATCTGGAACCTCTGTACCCAGGTGACCTTCATG
GCCGAAGGCCGGGTGATGCTCCGCGGTGAGCCGTCGGAGATCCGGGAACACGGGACCGTCGTCGAGAGCTACCTGGGCCA
GGGTCATGTCTGA

Upstream 100 bases:

>100_bases
GAGGTCCTGTTCGGACTGGCGCTCATCCTTGTCCTGATCTTCCGGCCGCTCGGCCTGCTCGGCCACATGCGGCGCGACCG
ACTCATGAGGAGGATCCACG

Downstream 100 bases:

>100_bases
GGCCACCAGGGTGCTCGAGGTCAGCGGCCTGCGAGCCGGCTACGACGGGATCGCCGTCCTCGACGAGGTCGACTTCACGG
TCGGCGACGAGGTCTTCGCG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPESSTTFRTELPTRTAPRPRLTLTDVSKSFAGRTVVDVEDLSLGDHPIEGLIGPNGAGKTTLMRLIMGSLRADTGQIRL
ERTGHHDVVLSQLRAHKIAQCGVVKTNQVITDFEGLTILDSLLLSVTPERREFPLHLKGERSTYDEHRAEIERQLDHFGL
GNTRRLALSAGEKKLVDIIRCLLLKPAVLLLDEPTAGLPAEVTQRVMDAVGGLAADGTTVVIIEHDLDVIWNLCTQVTFM
AEGRVMLRGEPSEIREHGTVVESYLGQGHV

Sequences:

>Translated_270_residues
MPESSTTFRTELPTRTAPRPRLTLTDVSKSFAGRTVVDVEDLSLGDHPIEGLIGPNGAGKTTLMRLIMGSLRADTGQIRL
ERTGHHDVVLSQLRAHKIAQCGVVKTNQVITDFEGLTILDSLLLSVTPERREFPLHLKGERSTYDEHRAEIERQLDHFGL
GNTRRLALSAGEKKLVDIIRCLLLKPAVLLLDEPTAGLPAEVTQRVMDAVGGLAADGTTVVIIEHDLDVIWNLCTQVTFM
AEGRVMLRGEPSEIREHGTVVESYLGQGHV
>Mature_269_residues
PESSTTFRTELPTRTAPRPRLTLTDVSKSFAGRTVVDVEDLSLGDHPIEGLIGPNGAGKTTLMRLIMGSLRADTGQIRLE
RTGHHDVVLSQLRAHKIAQCGVVKTNQVITDFEGLTILDSLLLSVTPERREFPLHLKGERSTYDEHRAEIERQLDHFGLG
NTRRLALSAGEKKLVDIIRCLLLKPAVLLLDEPTAGLPAEVTQRVMDAVGGLAADGTTVVIIEHDLDVIWNLCTQVTFMA
EGRVMLRGEPSEIREHGTVVESYLGQGHV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI46592964, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592956, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592978, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592971, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592915, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI46592898, Length=210, Percent_Identity=27.6190476190476, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI9955963, Length=246, Percent_Identity=28.8617886178862, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI215820660, Length=201, Percent_Identity=24.8756218905473, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI20143975, Length=201, Percent_Identity=24.8756218905473, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27881501, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=6e-11,
Organism=Homo sapiens, GI30795238, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=28.735632183908, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787792, Length=235, Percent_Identity=29.7872340425532, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789593, Length=246, Percent_Identity=29.2682926829268, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788897, Length=237, Percent_Identity=29.1139240506329, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788225, Length=245, Percent_Identity=28.9795918367347, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788472, Length=228, Percent_Identity=24.5614035087719, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790739, Length=237, Percent_Identity=29.535864978903, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787029, Length=242, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787370, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788761, Length=259, Percent_Identity=30.1158301158301, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI48995001, Length=220, Percent_Identity=29.5454545454545, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1790525, Length=245, Percent_Identity=25.3061224489796, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082268, Length=246, Percent_Identity=26.4227642276423, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789672, Length=238, Percent_Identity=27.3109243697479, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081709, Length=251, Percent_Identity=26.6932270916335, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787089, Length=226, Percent_Identity=28.7610619469027, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081782, Length=245, Percent_Identity=28.9795918367347, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786872, Length=227, Percent_Identity=25.9911894273128, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790544, Length=243, Percent_Identity=27.1604938271605, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790190, Length=249, Percent_Identity=24.0963855421687, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786319, Length=207, Percent_Identity=27.536231884058, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786398, Length=248, Percent_Identity=27.4193548387097, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788210, Length=253, Percent_Identity=23.3201581027668, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994997, Length=243, Percent_Identity=28.3950617283951, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788644, Length=87, Percent_Identity=34.4827586206897, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789873, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1790467, Length=245, Percent_Identity=28.1632653061224, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1786345, Length=238, Percent_Identity=26.890756302521, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=250, Percent_Identity=24.8, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25146777, Length=251, Percent_Identity=25.4980079681275, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI212646699, Length=243, Percent_Identity=24.2798353909465, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193211015, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323217, Length=259, Percent_Identity=27.7992277992278, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324498, Length=247, Percent_Identity=26.7206477732794, Blast_Score=62, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24581191, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581189, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581193, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24581195, Length=210, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI221512771, Length=236, Percent_Identity=22.0338983050847, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28573573, Length=238, Percent_Identity=24.7899159663866, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI28573571, Length=238, Percent_Identity=24.7899159663866, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI116007184, Length=232, Percent_Identity=23.2758620689655, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221500365, Length=232, Percent_Identity=23.2758620689655, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24659289, Length=208, Percent_Identity=28.3653846153846, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29648; Mature: 29516

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESSTTFRTELPTRTAPRPRLTLTDVSKSFAGRTVVDVEDLSLGDHPIEGLIGPNGAGK
CCCCCCEEEECCCCCCCCCCCEEHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCCH
TTLMRLIMGSLRADTGQIRLERTGHHDVVLSQLRAHKIAQCGVVKTNQVITDFEGLTILD
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCHHCCCHHHHHH
SLLLSVTPERREFPLHLKGERSTYDEHRAEIERQLDHFGLGNTRRLALSAGEKKLVDIIR
HHHHHCCCCHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
CLLLKPAVLLLDEPTAGLPAEVTQRVMDAVGGLAADGTTVVIIEHDLDVIWNLCTQVTFM
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHEEE
AEGRVMLRGEPSEIREHGTVVESYLGQGHV
ECCEEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PESSTTFRTELPTRTAPRPRLTLTDVSKSFAGRTVVDVEDLSLGDHPIEGLIGPNGAGK
CCCCCEEEECCCCCCCCCCCEEHHHHHHHHCCCEEEEHHHCCCCCCCCCCCCCCCCCCH
TTLMRLIMGSLRADTGQIRLERTGHHDVVLSQLRAHKIAQCGVVKTNQVITDFEGLTILD
HHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEECCCCHHCCCHHHHHH
SLLLSVTPERREFPLHLKGERSTYDEHRAEIERQLDHFGLGNTRRLALSAGEKKLVDIIR
HHHHHCCCCHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
CLLLKPAVLLLDEPTAGLPAEVTQRVMDAVGGLAADGTTVVIIEHDLDVIWNLCTQVTFM
HHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHEEE
AEGRVMLRGEPSEIREHGTVVESYLGQGHV
ECCEEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]