Click here to switch to the map view.

The map label for this gene is thcD [H]

Identifier: 119715077

GI number: 119715077

Start: 873799

End: 875076

Strand: Direct

Name: thcD [H]

Synonym: Noca_0832

Alternate gene names: 119715077

Gene position: 873799-875076 (Clockwise)

Preceding gene: 119715076

Following gene: 119715078

Centisome position: 17.53

GC content: 72.38

Gene sequence:

>1278_bases
GTGAGCGCCCGAACGCTGAGCGACAAGGTGGAGGTGGCTCAGCCGCACGCCGACGTACTCATCGTCGGCGGCGGCGAGGC
CGGGCTCGGCGTCGCCGACGAGCTGCGGGCACTGGGCTTCGCCGGCACCATCACCATGGCGGGCGAGGAGCCCAGGCTGC
CCTACCAGCGTCCTCCGCTGTCCAAGGGCTATCTCTCAGGAGACAGCGACGACGAGAGCCTGGAGCTGCGCGCCCCGGAG
TACCTGCGCGAGCAGGGCATCGACGTGTGGCTCGGGCGCCGAGTCGCCTCGGTCGACCTGACCGACCAGGGCGGCAGCGC
CGTCTTCGACGACTCCACCTCGGTGGCGTTCGACCGTCTGGTGCTCGCCACGGGCGCTGTCGCCCGGGAGCTGCCGGTCC
CTGGCGCCGAGCTGGCCGGGGTCCACTCGCTGCGAAGCGTCGCCGACGCCGCGCTCATTCGCGACGCCCTGCGAGCCGGC
GGCCCTCTCGTGGTGATCGGAGGCGGTTTCATCGGCCTCGAGATCGCCGCCACCGCCCGTGCGCGGGGGCTCTCGGTCAC
CGTGGTCGAGGCGGCCGGCCGACTCCTCGAGCGCGTCTGCGCCGAACCGCTCTCCGACTTCTGCTTGCGGACGCACCGTG
CCGCCGGCATCGACATCCGGCTCGACGCGGCCGTGGTCGAGCTCGTCGGGACCGACGTCGGGGCGGTCAGCGGCGTCCGG
CTCGCGGACGGGACGCTGTTGCCGGCCGAGGTCGTGATCGTCGGGGTCGGCGCGGTGCCGGCGACCGGGATCGCCGAGAA
GGCGGGCCTCGAGTGTCGCCGCGGAATCGTGGTGGACCCGGCAGGACGCACGACACACCCGCGCGTCGTCGCCGCGGGCG
ATTGCACCGAGCAACCCCACCCTCACCTGGAGGGGGAGCTGCTGGCGATCGAGTCGGTCAACAACGCCGTCGAGCAGTCG
AAGGCCGCCGCGCACACCCTGCTCGGCCTAGACCCGCCGGCACGTGGGGTGCCGTGGTTCTGGTCCGACCAGGGCGGCAT
GAAGATCCAGATCGCCGGCGTCTCACACGGCCACGACGGCTACGTGGTGCGCGAGGAGGCCGAGCGGATCACGGTCTTGT
ACTTCCGCGGAGGCACGCTGATCGCCGCGGATGTGGCCAACAACCCCCGCGACTTCATGGCCGTCAAGCGGGCCGTCGCC
GAACGGCGGACCGTGGACCGCGAGCGCGCTGGTGACCTGACTCGCTCCGTCAAGGACCTCCTCAGGGAAGGCGCCTGA

Upstream 100 bases:

>100_bases
ACGACGCGGAGACCCCGCGCACGCCGACGAGCAGACTGTCCTGCCAGATCCGCGTGACCGACGACCTCGACGGCCTGGAA
CTCACGATCGCGCCCGAGCA

Downstream 100 bases:

>100_bases
TGGCCTCCGCGCCGACCGGGGTCACGGTCACCACGCTCGAGCTGACGGTCGACCGAGACGCGCCGCTGCTGGTGGTCGGC
CCGTCGCTGGGCACCTCGGT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSARTLSDKVEVAQPHADVLIVGGGEAGLGVADELRALGFAGTITMAGEEPRLPYQRPPLSKGYLSGDSDDESLELRAPE
YLREQGIDVWLGRRVASVDLTDQGGSAVFDDSTSVAFDRLVLATGAVARELPVPGAELAGVHSLRSVADAALIRDALRAG
GPLVVIGGGFIGLEIAATARARGLSVTVVEAAGRLLERVCAEPLSDFCLRTHRAAGIDIRLDAAVVELVGTDVGAVSGVR
LADGTLLPAEVVIVGVGAVPATGIAEKAGLECRRGIVVDPAGRTTHPRVVAAGDCTEQPHPHLEGELLAIESVNNAVEQS
KAAAHTLLGLDPPARGVPWFWSDQGGMKIQIAGVSHGHDGYVVREEAERITVLYFRGGTLIAADVANNPRDFMAVKRAVA
ERRTVDRERAGDLTRSVKDLLREGA

Sequences:

>Translated_425_residues
MSARTLSDKVEVAQPHADVLIVGGGEAGLGVADELRALGFAGTITMAGEEPRLPYQRPPLSKGYLSGDSDDESLELRAPE
YLREQGIDVWLGRRVASVDLTDQGGSAVFDDSTSVAFDRLVLATGAVARELPVPGAELAGVHSLRSVADAALIRDALRAG
GPLVVIGGGFIGLEIAATARARGLSVTVVEAAGRLLERVCAEPLSDFCLRTHRAAGIDIRLDAAVVELVGTDVGAVSGVR
LADGTLLPAEVVIVGVGAVPATGIAEKAGLECRRGIVVDPAGRTTHPRVVAAGDCTEQPHPHLEGELLAIESVNNAVEQS
KAAAHTLLGLDPPARGVPWFWSDQGGMKIQIAGVSHGHDGYVVREEAERITVLYFRGGTLIAADVANNPRDFMAVKRAVA
ERRTVDRERAGDLTRSVKDLLREGA
>Mature_424_residues
SARTLSDKVEVAQPHADVLIVGGGEAGLGVADELRALGFAGTITMAGEEPRLPYQRPPLSKGYLSGDSDDESLELRAPEY
LREQGIDVWLGRRVASVDLTDQGGSAVFDDSTSVAFDRLVLATGAVARELPVPGAELAGVHSLRSVADAALIRDALRAGG
PLVVIGGGFIGLEIAATARARGLSVTVVEAAGRLLERVCAEPLSDFCLRTHRAAGIDIRLDAAVVELVGTDVGAVSGVRL
ADGTLLPAEVVIVGVGAVPATGIAEKAGLECRRGIVVDPAGRTTHPRVVAAGDCTEQPHPHLEGELLAIESVNNAVEQSK
AAAHTLLGLDPPARGVPWFWSDQGGMKIQIAGVSHGHDGYVVREEAERITVLYFRGGTLIAADVANNPRDFMAVKRAVAE
RRTVDRERAGDLTRSVKDLLREGA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=410, Percent_Identity=31.219512195122, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI65787454, Length=374, Percent_Identity=32.0855614973262, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI226437568, Length=374, Percent_Identity=32.0855614973262, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4757732, Length=367, Percent_Identity=25.8855585831063, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22202629, Length=369, Percent_Identity=25.7452574525745, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1788892, Length=404, Percent_Identity=34.4059405940594, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789065, Length=289, Percent_Identity=26.643598615917, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1789765, Length=281, Percent_Identity=27.7580071174377, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17559934, Length=334, Percent_Identity=29.940119760479, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564386, Length=288, Percent_Identity=26.0416666666667, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24639257, Length=341, Percent_Identity=29.6187683284457, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI281359715, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI281359713, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24639250, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI18543267, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24639252, Length=341, Percent_Identity=29.6187683284457, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24585130, Length=280, Percent_Identity=25.7142857142857, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI28573993, Length=367, Percent_Identity=25.8855585831063, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24581020, Length=367, Percent_Identity=25.8855585831063, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44467; Mature: 44336

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARTLSDKVEVAQPHADVLIVGGGEAGLGVADELRALGFAGTITMAGEEPRLPYQRPPL
CCCCCCCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCC
SKGYLSGDSDDESLELRAPEYLREQGIDVWLGRRVASVDLTDQGGSAVFDDSTSVAFDRL
CCCCCCCCCCCCCEEECCHHHHHHCCCEEEECCEEEEEEEECCCCCEEECCCCHHHHHHH
VLATGAVARELPVPGAELAGVHSLRSVADAALIRDALRAGGPLVVIGGGFIGLEIAATAR
HHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEECC
ARGLSVTVVEAAGRLLERVCAEPLSDFCLRTHRAAGIDIRLDAAVVELVGTDVGAVSGVR
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCE
LADGTLLPAEVVIVGVGAVPATGIAEKAGLECRRGIVVDPAGRTTHPRVVAAGDCTEQPH
EECCCCCCEEEEEEECCCCCCCCCHHHCCCHHHCCEEECCCCCCCCCEEEECCCCCCCCC
PHLEGELLAIESVNNAVEQSKAAAHTLLGLDPPARGVPWFWSDQGGMKIQIAGVSHGHDG
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCC
YVVREEAERITVLYFRGGTLIAADVANNPRDFMAVKRAVAERRTVDRERAGDLTRSVKDL
EEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREGA
HHCCC
>Mature Secondary Structure 
SARTLSDKVEVAQPHADVLIVGGGEAGLGVADELRALGFAGTITMAGEEPRLPYQRPPL
CCCCCCCCHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCC
SKGYLSGDSDDESLELRAPEYLREQGIDVWLGRRVASVDLTDQGGSAVFDDSTSVAFDRL
CCCCCCCCCCCCCEEECCHHHHHHCCCEEEECCEEEEEEEECCCCCEEECCCCHHHHHHH
VLATGAVARELPVPGAELAGVHSLRSVADAALIRDALRAGGPLVVIGGGFIGLEIAATAR
HHHHCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEEEECC
ARGLSVTVVEAAGRLLERVCAEPLSDFCLRTHRAAGIDIRLDAAVVELVGTDVGAVSGVR
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCE
LADGTLLPAEVVIVGVGAVPATGIAEKAGLECRRGIVVDPAGRTTHPRVVAAGDCTEQPH
EECCCCCCEEEEEEECCCCCCCCCHHHCCCHHHCCEEECCCCCCCCCEEEECCCCCCCCC
PHLEGELLAIESVNNAVEQSKAAAHTLLGLDPPARGVPWFWSDQGGMKIQIAGVSHGHDG
CCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCC
YVVREEAERITVLYFRGGTLIAADVANNPRDFMAVKRAVAERRTVDRERAGDLTRSVKDL
EEEEECCCEEEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LREGA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]