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The map label for this gene is purU [H]

Identifier: 119714891

GI number: 119714891

Start: 682018

End: 682872

Strand: Direct

Name: purU [H]

Synonym: Noca_0643

Alternate gene names: 119714891

Gene position: 682018-682872 (Clockwise)

Preceding gene: 119714890

Following gene: 119714893

Centisome position: 13.68

GC content: 61.87

Gene sequence:

>855_bases
GTGACGAACGACCTCATCCTTACCCTGTCCTGCAAGGACCGGCCCGGCATTGTCGCGGCCGTGGCCGGGTTCCTCGCGGA
TCGGGGCTTCTCCATCCGGGACAGCCAGCAATTCGGAGATGAAGACTCGGAGCTGTTCTTCATCCGTGTCCACGCTGCCA
GCGAGATGCCGGTTGAGTTCGACACGCTGCGAGCCGAGTTCGACACGACGATCGGCAGCGTCCTAGAGGCGTCCTGGGCA
TTGCATGATCCGTCCGTCAAGCACCGATTACTTCTTATGGTGTCCCGCCAGGGTCACTGCCTCAACGATCTGCTGCATCG
CGTGCGAACCGGATCGCTAGCGGCAGACGTCGTCGCTATTGTCTCTAATCACGAGGACTTCCGAGAACTCGTCGAGTGGC
ATGGCATCCCGTTCCACCATGTCCCCGTCACCGCGGAGTCCAAGGACTGGGCCGAGGACGAGTTGAGGAAACTCGTCGCG
GCGTACGACGCCGACTCGGTCATCCTGGCTCGGTACATGCAGATCCTCTCGGACAGTCTTTGCCGAGACTTGGCAGGTCG
CGCTATCAACATCCACCACAGCCTGCTCCCCAGCTTCAAGGGCGCTCGGCCGTATTACCAGGCGCACGCGCGCGGAGTGA
AGGTCATCGGCGCCACGGCACACTACGTGACTGCAGATCTAGACGAGGGCCCGATCATCGAACAGGACTTCATTCGCGTC
GACCACTCGAAGTCTGCGGCAGACCTCACGGCAATCGGTCGCGATCTCGAAGCCCTTGCCCTCGCCCGTGCTGTCACCGC
TCACACGGAGCACCGCGTACTGATGAACGGGTCCCGAACGGTGGTGTTCCGATGA

Upstream 100 bases:

>100_bases
TCGAGTCCGAGCCGTACGAGATCGAGGTAGGCGGCCGGCGCGTCCCCGCTCGAGCTTCCTTGAGTCCCATGTATGACCCG
AAGTCCGAAAGGCCCAAGCA

Downstream 100 bases:

>100_bases
CGATCACCGGGGCATCGATGAGCACCGCGAACTCGTCCGACGCTGGCGCGCACTCTGAAGCCGATGATGAAGTAAGCCGC
GACGGCATCACTATCGGCAG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTNDLILTLSCKDRPGIVAAVAGFLADRGFSIRDSQQFGDEDSELFFIRVHAASEMPVEFDTLRAEFDTTIGSVLEASWA
LHDPSVKHRLLLMVSRQGHCLNDLLHRVRTGSLAADVVAIVSNHEDFRELVEWHGIPFHHVPVTAESKDWAEDELRKLVA
AYDADSVILARYMQILSDSLCRDLAGRAINIHHSLLPSFKGARPYYQAHARGVKVIGATAHYVTADLDEGPIIEQDFIRV
DHSKSAADLTAIGRDLEALALARAVTAHTEHRVLMNGSRTVVFR

Sequences:

>Translated_284_residues
MTNDLILTLSCKDRPGIVAAVAGFLADRGFSIRDSQQFGDEDSELFFIRVHAASEMPVEFDTLRAEFDTTIGSVLEASWA
LHDPSVKHRLLLMVSRQGHCLNDLLHRVRTGSLAADVVAIVSNHEDFRELVEWHGIPFHHVPVTAESKDWAEDELRKLVA
AYDADSVILARYMQILSDSLCRDLAGRAINIHHSLLPSFKGARPYYQAHARGVKVIGATAHYVTADLDEGPIIEQDFIRV
DHSKSAADLTAIGRDLEALALARAVTAHTEHRVLMNGSRTVVFR
>Mature_283_residues
TNDLILTLSCKDRPGIVAAVAGFLADRGFSIRDSQQFGDEDSELFFIRVHAASEMPVEFDTLRAEFDTTIGSVLEASWAL
HDPSVKHRLLLMVSRQGHCLNDLLHRVRTGSLAADVVAIVSNHEDFRELVEWHGIPFHHVPVTAESKDWAEDELRKLVAA
YDADSVILARYMQILSDSLCRDLAGRAINIHHSLLPSFKGARPYYQAHARGVKVIGATAHYVTADLDEGPIIEQDFIRVD
HSKSAADLTAIGRDLEALALARAVTAHTEHRVLMNGSRTVVFR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=167, Percent_Identity=33.5329341317365, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869995, Length=167, Percent_Identity=33.5329341317365, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869993, Length=167, Percent_Identity=33.5329341317365, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=37.9432624113475, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1788846, Length=166, Percent_Identity=33.1325301204819, Blast_Score=81, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=159, Percent_Identity=35.8490566037736, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24582400, Length=163, Percent_Identity=31.9018404907975, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31509; Mature: 31378

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDLILTLSCKDRPGIVAAVAGFLADRGFSIRDSQQFGDEDSELFFIRVHAASEMPVEF
CCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCHH
DTLRAEFDTTIGSVLEASWALHDPSVKHRLLLMVSRQGHCLNDLLHRVRTGSLAADVVAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
VSNHEDFRELVEWHGIPFHHVPVTAESKDWAEDELRKLVAAYDADSVILARYMQILSDSL
HCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CRDLAGRAINIHHSLLPSFKGARPYYQAHARGVKVIGATAHYVTADLDEGPIIEQDFIRV
HHHHCCCEEEEHHHHCCCCCCCCCHHHHHCCCEEEEEEEEEEEEEECCCCCCCHHHHEEE
DHSKSAADLTAIGRDLEALALARAVTAHTEHRVLMNGSRTVVFR
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCEEEEEC
>Mature Secondary Structure 
TNDLILTLSCKDRPGIVAAVAGFLADRGFSIRDSQQFGDEDSELFFIRVHAASEMPVEF
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEEEEECCCCCCCHH
DTLRAEFDTTIGSVLEASWALHDPSVKHRLLLMVSRQGHCLNDLLHRVRTGSLAADVVAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHEEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHH
VSNHEDFRELVEWHGIPFHHVPVTAESKDWAEDELRKLVAAYDADSVILARYMQILSDSL
HCCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
CRDLAGRAINIHHSLLPSFKGARPYYQAHARGVKVIGATAHYVTADLDEGPIIEQDFIRV
HHHHCCCEEEEHHHHCCCCCCCCCHHHHHCCCEEEEEEEEEEEEEECCCCCCCHHHHEEE
DHSKSAADLTAIGRDLEALALARAVTAHTEHRVLMNGSRTVVFR
CCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]