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The map label for this gene is nuoM [H]

Identifier: 119714796

GI number: 119714796

Start: 563712

End: 565241

Strand: Direct

Name: nuoM [H]

Synonym: Noca_0532

Alternate gene names: 119714796

Gene position: 563712-565241 (Clockwise)

Preceding gene: 119714795

Following gene: 119714797

Centisome position: 11.31

GC content: 68.89

Gene sequence:

>1530_bases
ATGAACGACCTTCCCTGGCTCACCGCGCTCTGGGTGCTGCCGCTGGTCGGCGCCCTGGTGACGGCGTTCGTGCCCCGGGC
GACCGGGTCGGCGCTGCCCAAGCAGGTCGGGCTGCTGTTCGCGCTCCTCACGCTCGCCGTGGGCATCGCGATCGCCGTCC
AGTACGACACCGGCGACGGCATGCAGCTGACCGAGACGCACACCTGGATCGAGGCCTTCGGCGTCAACTACGCCCTGGGC
GTCGACGGCCTCGGGCTGCTGCTCATCCTGATGACGGTCGCCCTGGTCCCGTTGGTGCTGGTGGCCACCTGGAACGACGC
CGACGACCGCTCGTCCGCGGCCTTCGTGGCCTGGGCGCTCGCGCTCGAGTCGTTCTCCCTCGCGGTGTTCGCCGCCACCG
ACGTCTTCCTCTTCTACATGGTCTTCGAGGCGACGCTGATCCCGGCGTACTTCCTCATCGGCCGCTTCGGCCAGGCCGGT
CGCGGTCGCGCCGCGCTGAAGTTCCTCATGTATCAGCTCGGCGGCGGCCTGGTGCTGCTGGCGTCGGTGATCGGGCTCTA
CGTCGTCTCGGCCGACGCCGGCAACCCGTCCTACCTGCTCTCCGACCTGCAGCAGCTCGACCTGAGCACCAACACCGAGC
GCTGGCTGTTCGTCGGGTTCATGATCGCGTTCGCGGTGAAGGCCCCGCTGTTCCCGCTGCACACCTGGCTGGCCGACACG
ACCGAGAAGGCGACGCCCGGCACCAGCGTGCTGCTGGTCTGCATTCTCGACAAGATCGGCACCTTCGGGATGCTGCGCTT
CGGCCTGGGCCTGTTCCCGGAGGCCTCGCAGTGGGCGACGCCGGTCGTCGTCGCGCTCGCGCTGGTCTCGATCGTGTACG
GCGCGCTGGTCGCGATCGGCCAGGACGACATCCTGCGCCTGATCGGCCTGACCTCGCTGAGCCACTTCGGCTTCATCACC
CTGGGCATCTTCGCGTTCAGCAGCCAGGGCGGGTCCGGCGCGATCCTCTACATGGTCAACCACGGTGTCGGCACGGCGGC
GCTCTTCCTGATCGCCGGCTACCTGATCCGGCGCAAGGGCACCCAGCTGATCAGCCAGATCGCCGGCGTCGAGACCAAGG
CGCCGCTGCTCGCCGGGCTGTTCCTGGTCGCCGGGCTCTCCACGCTGGGCCTGCCTGGCCTGAGCCAGTTCGTCTCCGAG
ATCCTCGTGCTGATCTCGGCCTTCGACTACCACTGGTGGGTCGGCGCGATCGCCGTCACGGGCATCGTGCTCGCCGCGAT
CTACGTGCTCTGGCTCTACCAGCGCACGATGACCGGGCCGGCGACGCCGGGCGACGAGGCGATGACCGACCTGAACCGGC
GCGAGCTGGTCGCGGTGGTGCCCCTGATCGCCGCGCTGGTGTTCTTCGGCTTCTTCCCGATGCCACTGCTCGACGTCAGC
AATCCGACGGTCGACTCCCTGCTGCAACACGTCGGCGTGTCCGACGAGGCTCCGACGGTCGCTGACCACGCCGAGGAAGG
TGCGCACTGA

Upstream 100 bases:

>100_bases
TCGCCCGGCGGGTCCAGAACGGCTTCGTCCGCACCTACGCCCTGTCCCTCCTCGGCGGCGCCCTCATCGTCGTCCTCGCT
CTCCTGGCGGTGAATCTCGC

Downstream 100 bases:

>100_bases
TGGACTTCATCAAGCCCACGATCGAGTACGGCGACGTCTGGCCGCTCCTCGTGGTCTTCGGCGTCGCCGCCGTCGGCGTG
CTCGTCGAGGGGTTCGTGCC

Product: proton-translocating NADH-quinone oxidoreductase, chain M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNDLPWLTALWVLPLVGALVTAFVPRATGSALPKQVGLLFALLTLAVGIAIAVQYDTGDGMQLTETHTWIEAFGVNYALG
VDGLGLLLILMTVALVPLVLVATWNDADDRSSAAFVAWALALESFSLAVFAATDVFLFYMVFEATLIPAYFLIGRFGQAG
RGRAALKFLMYQLGGGLVLLASVIGLYVVSADAGNPSYLLSDLQQLDLSTNTERWLFVGFMIAFAVKAPLFPLHTWLADT
TEKATPGTSVLLVCILDKIGTFGMLRFGLGLFPEASQWATPVVVALALVSIVYGALVAIGQDDILRLIGLTSLSHFGFIT
LGIFAFSSQGGSGAILYMVNHGVGTAALFLIAGYLIRRKGTQLISQIAGVETKAPLLAGLFLVAGLSTLGLPGLSQFVSE
ILVLISAFDYHWWVGAIAVTGIVLAAIYVLWLYQRTMTGPATPGDEAMTDLNRRELVAVVPLIAALVFFGFFPMPLLDVS
NPTVDSLLQHVGVSDEAPTVADHAEEGAH

Sequences:

>Translated_509_residues
MNDLPWLTALWVLPLVGALVTAFVPRATGSALPKQVGLLFALLTLAVGIAIAVQYDTGDGMQLTETHTWIEAFGVNYALG
VDGLGLLLILMTVALVPLVLVATWNDADDRSSAAFVAWALALESFSLAVFAATDVFLFYMVFEATLIPAYFLIGRFGQAG
RGRAALKFLMYQLGGGLVLLASVIGLYVVSADAGNPSYLLSDLQQLDLSTNTERWLFVGFMIAFAVKAPLFPLHTWLADT
TEKATPGTSVLLVCILDKIGTFGMLRFGLGLFPEASQWATPVVVALALVSIVYGALVAIGQDDILRLIGLTSLSHFGFIT
LGIFAFSSQGGSGAILYMVNHGVGTAALFLIAGYLIRRKGTQLISQIAGVETKAPLLAGLFLVAGLSTLGLPGLSQFVSE
ILVLISAFDYHWWVGAIAVTGIVLAAIYVLWLYQRTMTGPATPGDEAMTDLNRRELVAVVPLIAALVFFGFFPMPLLDVS
NPTVDSLLQHVGVSDEAPTVADHAEEGAH
>Mature_509_residues
MNDLPWLTALWVLPLVGALVTAFVPRATGSALPKQVGLLFALLTLAVGIAIAVQYDTGDGMQLTETHTWIEAFGVNYALG
VDGLGLLLILMTVALVPLVLVATWNDADDRSSAAFVAWALALESFSLAVFAATDVFLFYMVFEATLIPAYFLIGRFGQAG
RGRAALKFLMYQLGGGLVLLASVIGLYVVSADAGNPSYLLSDLQQLDLSTNTERWLFVGFMIAFAVKAPLFPLHTWLADT
TEKATPGTSVLLVCILDKIGTFGMLRFGLGLFPEASQWATPVVVALALVSIVYGALVAIGQDDILRLIGLTSLSHFGFIT
LGIFAFSSQGGSGAILYMVNHGVGTAALFLIAGYLIRRKGTQLISQIAGVETKAPLLAGLFLVAGLSTLGLPGLSQFVSE
ILVLISAFDYHWWVGAIAVTGIVLAAIYVLWLYQRTMTGPATPGDEAMTDLNRRELVAVVPLIAALVFFGFFPMPLLDVS
NPTVDSLLQHVGVSDEAPTVADHAEEGAH

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=376, Percent_Identity=30.5851063829787, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI251831117, Length=409, Percent_Identity=22.9828850855746, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788613, Length=505, Percent_Identity=33.4653465346535, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788827, Length=288, Percent_Identity=25.3472222222222, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788829, Length=421, Percent_Identity=25.8907363420428, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788831, Length=292, Percent_Identity=26.027397260274, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI145693160, Length=362, Percent_Identity=27.6243093922652, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788614, Length=397, Percent_Identity=24.4332493702771, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 54099; Mature: 54099

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLPWLTALWVLPLVGALVTAFVPRATGSALPKQVGLLFALLTLAVGIAIAVQYDTGDG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCC
MQLTETHTWIEAFGVNYALGVDGLGLLLILMTVALVPLVLVATWNDADDRSSAAFVAWAL
CEEHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALESFSLAVFAATDVFLFYMVFEATLIPAYFLIGRFGQAGRGRAALKFLMYQLGGGLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHH
ASVIGLYVVSADAGNPSYLLSDLQQLDLSTNTERWLFVGFMIAFAVKAPLFPLHTWLADT
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
TEKATPGTSVLLVCILDKIGTFGMLRFGLGLFPEASQWATPVVVALALVSIVYGALVAIG
CCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QDDILRLIGLTSLSHFGFITLGIFAFSSQGGSGAILYMVNHGVGTAALFLIAGYLIRRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
TQLISQIAGVETKAPLLAGLFLVAGLSTLGLPGLSQFVSEILVLISAFDYHWWVGAIAVT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIVLAAIYVLWLYQRTMTGPATPGDEAMTDLNRRELVAVVPLIAALVFFGFFPMPLLDVS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NPTVDSLLQHVGVSDEAPTVADHAEEGAH
CCCHHHHHHHCCCCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MNDLPWLTALWVLPLVGALVTAFVPRATGSALPKQVGLLFALLTLAVGIAIAVQYDTGDG
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCC
MQLTETHTWIEAFGVNYALGVDGLGLLLILMTVALVPLVLVATWNDADDRSSAAFVAWAL
CEEHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALESFSLAVFAATDVFLFYMVFEATLIPAYFLIGRFGQAGRGRAALKFLMYQLGGGLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHH
ASVIGLYVVSADAGNPSYLLSDLQQLDLSTNTERWLFVGFMIAFAVKAPLFPLHTWLADT
HHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCC
TEKATPGTSVLLVCILDKIGTFGMLRFGLGLFPEASQWATPVVVALALVSIVYGALVAIG
CCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
QDDILRLIGLTSLSHFGFITLGIFAFSSQGGSGAILYMVNHGVGTAALFLIAGYLIRRKG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHH
TQLISQIAGVETKAPLLAGLFLVAGLSTLGLPGLSQFVSEILVLISAFDYHWWVGAIAVT
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIVLAAIYVLWLYQRTMTGPATPGDEAMTDLNRRELVAVVPLIAALVFFGFFPMPLLDVS
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
NPTVDSLLQHVGVSDEAPTVADHAEEGAH
CCCHHHHHHHCCCCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]