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The map label for this gene is thiB [H]

Identifier: 119714697

GI number: 119714697

Start: 462413

End: 463492

Strand: Direct

Name: thiB [H]

Synonym: Noca_0433

Alternate gene names: 119714697

Gene position: 462413-463492 (Clockwise)

Preceding gene: 119714696

Following gene: 119714698

Centisome position: 9.27

GC content: 70.83

Gene sequence:

>1080_bases
ATGAGACCCATCCATGCCCTGGCGCCACTGGCACCGCTCGTCGCGCTCGCCCTGGCGGCGACCGGCTGCTCGACCATCGG
AAACGGCTCGTCCGGCGACGAGTCGACGGCCAGCCCGGGCGGCGAGCCCGGGCGGGTCGTGCTGGTCACCCACGAGTCGT
TCTCGCTGCCCAAGCCGCTGATCAAGCAGTTCGAGCAGCAGTCCGGCTACGACCTGGTGGTCCGCGCGTCCGGCGACGCC
GGGATCCTCACCAACAAGCTGGTGCTGACCAAGGGCGACCCGCTCGGCGACGTGGCGTTCGGCATCGACAACACCTTCGC
CTCGCGGGCGCTCGACGAGGGCGTGTTCGCGCCGTACGACGCTCCGCTGCCGGACGGCGCCGACCAGTACCGGTTGCCGG
GTGACGACGAGCACGACCTCACGCCGATCGACAACGCCAGCGTGTGCGTGAACGTCGACGACACCTGGTTCGCCGACCAC
CATCTCGCCCCGCCGAAGACCCTGGACGACCTGGCCGCCCCGGAGTACCAGGGCCTGTTCGTCACCCCGGGCGCGGCCAC
CAGCTCGCCCGGCCTGGCGTTCCTGCTGAGCACGATCGCGGCGTACGGCGAGGACGGCTGGCAGGACTACTGGGCGCGGC
TGATGGAGAACGGGACCAAGCTGACGGCCGGCTGGTCCGACGCCTACGAGGTCGACTTCACCCAGGGCGGCGGCCAGGGT
GACCGGCCGATCGTGCTGTCCTACGACTCCTCGCCGGCGTTCACCGTCGCCGACGGGGAGTCGTCGACGAGCGCCCTGCT
CGACACCTGCTTCCGCCAGGTGGAGTACGCCGGCGTGCTGGACGGTGCCGCGAACCCCGCCGGCGCCGAGCAGCTGGTCG
ACTTCCTGCTCTCGCCGGAGGTGCAGGCCGCACTGCCCGAGAGCATGTACGTGTTCCCGGTCGACTCCTCGGTCCAGCTG
CCGAAGGAGTGGGCCCGGTTCGCGAAGCAGCCGAGCGACCCGTTCTCGGTGGACCCCGCGTCGATCGACGAGCACCGCGA
CGAGTGGCTGCGCGAGTGGACCGACGTGACCTCTCGATGA

Upstream 100 bases:

>100_bases
CAAACGCAGGGGAGCACGCCTCAACAACGTGCTGAGAGTGCGGATCCGCCGCAGACCCTATGAACCTGATCCGGTTAGCA
CCGGCGAAGGGAGTCGGAAG

Downstream 100 bases:

>100_bases
CCGGCACCGCGCGGACGGGGTGACGGGCAGGCCATGAGGCGCTCGCTCGAGCTGGTCGGGCTCGCGGCGCTGCCGCTGGC
CGTGCTCGGCGTGTTCTTCC

Product: ABC transporter periplasmic-binding protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MRPIHALAPLAPLVALALAATGCSTIGNGSSGDESTASPGGEPGRVVLVTHESFSLPKPLIKQFEQQSGYDLVVRASGDA
GILTNKLVLTKGDPLGDVAFGIDNTFASRALDEGVFAPYDAPLPDGADQYRLPGDDEHDLTPIDNASVCVNVDDTWFADH
HLAPPKTLDDLAAPEYQGLFVTPGAATSSPGLAFLLSTIAAYGEDGWQDYWARLMENGTKLTAGWSDAYEVDFTQGGGQG
DRPIVLSYDSSPAFTVADGESSTSALLDTCFRQVEYAGVLDGAANPAGAEQLVDFLLSPEVQAALPESMYVFPVDSSVQL
PKEWARFAKQPSDPFSVDPASIDEHRDEWLREWTDVTSR

Sequences:

>Translated_359_residues
MRPIHALAPLAPLVALALAATGCSTIGNGSSGDESTASPGGEPGRVVLVTHESFSLPKPLIKQFEQQSGYDLVVRASGDA
GILTNKLVLTKGDPLGDVAFGIDNTFASRALDEGVFAPYDAPLPDGADQYRLPGDDEHDLTPIDNASVCVNVDDTWFADH
HLAPPKTLDDLAAPEYQGLFVTPGAATSSPGLAFLLSTIAAYGEDGWQDYWARLMENGTKLTAGWSDAYEVDFTQGGGQG
DRPIVLSYDSSPAFTVADGESSTSALLDTCFRQVEYAGVLDGAANPAGAEQLVDFLLSPEVQAALPESMYVFPVDSSVQL
PKEWARFAKQPSDPFSVDPASIDEHRDEWLREWTDVTSR
>Mature_359_residues
MRPIHALAPLAPLVALALAATGCSTIGNGSSGDESTASPGGEPGRVVLVTHESFSLPKPLIKQFEQQSGYDLVVRASGDA
GILTNKLVLTKGDPLGDVAFGIDNTFASRALDEGVFAPYDAPLPDGADQYRLPGDDEHDLTPIDNASVCVNVDDTWFADH
HLAPPKTLDDLAAPEYQGLFVTPGAATSSPGLAFLLSTIAAYGEDGWQDYWARLMENGTKLTAGWSDAYEVDFTQGGGQG
DRPIVLSYDSSPAFTVADGESSTSALLDTCFRQVEYAGVLDGAANPAGAEQLVDFLLSPEVQAALPESMYVFPVDSSVQL
PKEWARFAKQPSDPFSVDPASIDEHRDEWLREWTDVTSR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=322, Percent_Identity=27.9503105590062, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38224; Mature: 38224

Theoretical pI: Translated: 3.95; Mature: 3.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPIHALAPLAPLVALALAATGCSTIGNGSSGDESTASPGGEPGRVVLVTHESFSLPKPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHH
IKQFEQQSGYDLVVRASGDAGILTNKLVLTKGDPLGDVAFGIDNTFASRALDEGVFAPYD
HHHHHHCCCCEEEEEECCCCCEEECEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCCCC
APLPDGADQYRLPGDDEHDLTPIDNASVCVNVDDTWFADHHLAPPKTLDDLAAPEYQGLF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHCCCCCCCEE
VTPGAATSSPGLAFLLSTIAAYGEDGWQDYWARLMENGTKLTAGWSDAYEVDFTQGGGQG
EECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCC
DRPIVLSYDSSPAFTVADGESSTSALLDTCFRQVEYAGVLDGAANPAGAEQLVDFLLSPE
CCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
VQAALPESMYVFPVDSSVQLPKEWARFAKQPSDPFSVDPASIDEHRDEWLREWTDVTSR
HHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRPIHALAPLAPLVALALAATGCSTIGNGSSGDESTASPGGEPGRVVLVTHESFSLPKPL
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHH
IKQFEQQSGYDLVVRASGDAGILTNKLVLTKGDPLGDVAFGIDNTFASRALDEGVFAPYD
HHHHHHCCCCEEEEEECCCCCEEECEEEEECCCCCCCEECCCCHHHHHHHHHCCCCCCCC
APLPDGADQYRLPGDDEHDLTPIDNASVCVNVDDTWFADHHLAPPKTLDDLAAPEYQGLF
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCCCCCHHHHCCCCCCCEE
VTPGAATSSPGLAFLLSTIAAYGEDGWQDYWARLMENGTKLTAGWSDAYEVDFTQGGGQG
EECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCCCCEEEEECCCCCCC
DRPIVLSYDSSPAFTVADGESSTSALLDTCFRQVEYAGVLDGAANPAGAEQLVDFLLSPE
CCCEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCC
VQAALPESMYVFPVDSSVQLPKEWARFAKQPSDPFSVDPASIDEHRDEWLREWTDVTSR
HHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]