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The map label for this gene is thiP [H]

Identifier: 119714698

GI number: 119714698

Start: 463526

End: 465160

Strand: Direct

Name: thiP [H]

Synonym: Noca_0434

Alternate gene names: 119714698

Gene position: 463526-465160 (Clockwise)

Preceding gene: 119714697

Following gene: 119714699

Centisome position: 9.3

GC content: 75.05

Gene sequence:

>1635_bases
ATGAGGCGCTCGCTCGAGCTGGTCGGGCTCGCGGCGCTGCCGCTGGCCGTGCTCGGCGTGTTCTTCCTGCTCCCGGTCAG
CGGCATGGTCTCCGAGGGCTTCGTCACCGACGGCCGCTTCGACCCGGGTGGGGTGCTCGAGGTGCTCGGCCGGCCCCGGG
TGCACCGGGTGCTGTGGTTCACGGTCTGGTCCTCGACGGTCGCGACCCTGCTCGCCGTGCTCCTCGGCCTGCCCGTGGCG
CACCTGCTGCACCGGCGCCGGTTCTGGGGCCGGCGGGCGCTGCGCTCGCTGCTGCTGGTGCCGTTCGTGCTGCCGACGGT
GGTGGTCGGGGTCGCGTTCCGCGAGCTGATCGGCGAGGCCGGGCCGTTGGGCTTCCTCGGCCTGGACGGCTCGGCCGTGG
CCATCGTCGCCGGCCTCGTGTTCTTCAACGTGGCGGTCGTGATCCGGGCGGTCGGCGCCGCCTGGGAGTCCCTCGACCCG
CGGCCGGCGGAGGCGGCCGCGGCGCTCGGTGCGGGGCCGGCCCACGTCTTCCGGACCGTCACCCTGCCCGCGCTGCGCCC
GGCGATCGTCAGCGCCGCCAGCGTGGTCTTCCTCTTCTGCGCCACCGCGTTCGGCGTGGTGCTCACCCTGGGCGGGCTGC
GCTACTCCTCGGTCGAGACCGAGATCTACCTGCTCACCACGAACCTGCTCGACCTCCAGGCCGCGGCGGCGCTCTCGATC
CTCCAGCTGCTCGCGATCACGGCCCTGCTGGTGGTGACCGGCCGGTTGCGCGCCGTACCCGACCCGGCTCTGGCCCGCTC
CACCCGGCGCCCGCCGCGGCCGGCGCCCGGCGACTGGCCCGGCCTGGTCGCGACCGGCCTGGTGCTGCTGCTGGTGGGGG
CGCCGGTGGCCGCCCTGGTCGCCGGCTCGCTGCGGGTCGGCGACGCGTGGAGCCTGGGCAACTACCGCGCGCTGACCGGC
GAGGGCACGCAGCACGCCCTGCTCGCCTCCGCGACCGACGCGCTCGTCACCTCGCTGCGCACCGCCGTCGACGCCACCTG
GATGTCGCTGTCCCTGGGCGTGCTGGTCACCGTGGTGGTCACCCGCCGGTCGCGCACGCGGGGTGAGCGCCGGGCGCGCG
GCCTGCTGGACGGGCTGTTCATGCTCCCGCTCGGGGTGTCCGCGGTGACGCTCGGCTTCGGGTTCCTCATCACCCTCGAC
CGGCCGCCCCTCGACCTGAGGGACTCACCGCTCCTGGTCCCGATCGCGCAGGCGCTGGTGGCGCTGCCGCTCGTCGTCCG
GACCCTGGTGCCGGTGCTCGGTGGCATCGACGACCGCCAGCGGCAGGCCGCCGCGTCGCTCGGTGCCGGCCCGTTGCGTA
CCTTCGCCACCGTGGACCTCCCCGCCATGTGGCGGCCGCTGACCGCCGCGGCCGGCTTCGCGTTCGCGGTGTCGCTCGGT
GAGTTCGGGGCGACCTCGTTCCTCGCCCGGGAGGACCACCCCACGCTGCCGATCGTGATCTACCACCTGATCGGGCACCC
GGGTGCGATGAACTACGGCATGGCCCTCGCCGCGTCCGTCGTCCTCGCCACCACGACCGCGCTCGTGATGCTCGCCGTCG
ACCGGCTGCGGGTGCCGTCGGTGGGGGCGTTCTGA

Upstream 100 bases:

>100_bases
CCCCGCGTCGATCGACGAGCACCGCGACGAGTGGCTGCGCGAGTGGACCGACGTGACCTCTCGATGACCGGCACCGCGCG
GACGGGGTGACGGGCAGGCC

Downstream 100 bases:

>100_bases
TGCTGGCCCTGGACCACCTCAGCGTCTCGTACGACGGCGTGCCGGCTGTCGTCGACGCGACGCTGGAGCTCGCGGACGGG
CACGTCCTCGCGGTCCTGGG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MRRSLELVGLAALPLAVLGVFFLLPVSGMVSEGFVTDGRFDPGGVLEVLGRPRVHRVLWFTVWSSTVATLLAVLLGLPVA
HLLHRRRFWGRRALRSLLLVPFVLPTVVVGVAFRELIGEAGPLGFLGLDGSAVAIVAGLVFFNVAVVIRAVGAAWESLDP
RPAEAAAALGAGPAHVFRTVTLPALRPAIVSAASVVFLFCATAFGVVLTLGGLRYSSVETEIYLLTTNLLDLQAAAALSI
LQLLAITALLVVTGRLRAVPDPALARSTRRPPRPAPGDWPGLVATGLVLLLVGAPVAALVAGSLRVGDAWSLGNYRALTG
EGTQHALLASATDALVTSLRTAVDATWMSLSLGVLVTVVVTRRSRTRGERRARGLLDGLFMLPLGVSAVTLGFGFLITLD
RPPLDLRDSPLLVPIAQALVALPLVVRTLVPVLGGIDDRQRQAAASLGAGPLRTFATVDLPAMWRPLTAAAGFAFAVSLG
EFGATSFLAREDHPTLPIVIYHLIGHPGAMNYGMALAASVVLATTTALVMLAVDRLRVPSVGAF

Sequences:

>Translated_544_residues
MRRSLELVGLAALPLAVLGVFFLLPVSGMVSEGFVTDGRFDPGGVLEVLGRPRVHRVLWFTVWSSTVATLLAVLLGLPVA
HLLHRRRFWGRRALRSLLLVPFVLPTVVVGVAFRELIGEAGPLGFLGLDGSAVAIVAGLVFFNVAVVIRAVGAAWESLDP
RPAEAAAALGAGPAHVFRTVTLPALRPAIVSAASVVFLFCATAFGVVLTLGGLRYSSVETEIYLLTTNLLDLQAAAALSI
LQLLAITALLVVTGRLRAVPDPALARSTRRPPRPAPGDWPGLVATGLVLLLVGAPVAALVAGSLRVGDAWSLGNYRALTG
EGTQHALLASATDALVTSLRTAVDATWMSLSLGVLVTVVVTRRSRTRGERRARGLLDGLFMLPLGVSAVTLGFGFLITLD
RPPLDLRDSPLLVPIAQALVALPLVVRTLVPVLGGIDDRQRQAAASLGAGPLRTFATVDLPAMWRPLTAAAGFAFAVSLG
EFGATSFLAREDHPTLPIVIYHLIGHPGAMNYGMALAASVVLATTTALVMLAVDRLRVPSVGAF
>Mature_544_residues
MRRSLELVGLAALPLAVLGVFFLLPVSGMVSEGFVTDGRFDPGGVLEVLGRPRVHRVLWFTVWSSTVATLLAVLLGLPVA
HLLHRRRFWGRRALRSLLLVPFVLPTVVVGVAFRELIGEAGPLGFLGLDGSAVAIVAGLVFFNVAVVIRAVGAAWESLDP
RPAEAAAALGAGPAHVFRTVTLPALRPAIVSAASVVFLFCATAFGVVLTLGGLRYSSVETEIYLLTTNLLDLQAAAALSI
LQLLAITALLVVTGRLRAVPDPALARSTRRPPRPAPGDWPGLVATGLVLLLVGAPVAALVAGSLRVGDAWSLGNYRALTG
EGTQHALLASATDALVTSLRTAVDATWMSLSLGVLVTVVVTRRSRTRGERRARGLLDGLFMLPLGVSAVTLGFGFLITLD
RPPLDLRDSPLLVPIAQALVALPLVVRTLVPVLGGIDDRQRQAAASLGAGPLRTFATVDLPAMWRPLTAAAGFAFAVSLG
EFGATSFLAREDHPTLPIVIYHLIGHPGAMNYGMALAASVVLATTTALVMLAVDRLRVPSVGAF

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=464, Percent_Identity=29.7413793103448, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1788764, Length=200, Percent_Identity=30.5, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1787570, Length=266, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87082099, Length=244, Percent_Identity=27.4590163934426, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56962; Mature: 56962

Theoretical pI: Translated: 11.62; Mature: 11.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRSLELVGLAALPLAVLGVFFLLPVSGMVSEGFVTDGRFDPGGVLEVLGRPRVHRVLWF
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHH
TVWSSTVATLLAVLLGLPVAHLLHRRRFWGRRALRSLLLVPFVLPTVVVGVAFRELIGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPLGFLGLDGSAVAIVAGLVFFNVAVVIRAVGAAWESLDPRPAEAAAALGAGPAHVFRTV
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHH
TLPALRPAIVSAASVVFLFCATAFGVVLTLGGLRYSSVETEIYLLTTNLLDLQAAAALSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCHHHHHHHHHHHH
LQLLAITALLVVTGRLRAVPDPALARSTRRPPRPAPGDWPGLVATGLVLLLVGAPVAALV
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHH
AGSLRVGDAWSLGNYRALTGEGTQHALLASATDALVTSLRTAVDATWMSLSLGVLVTVVV
HCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRSRTRGERRARGLLDGLFMLPLGVSAVTLGFGFLITLDRPPLDLRDSPLLVPIAQALV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ALPLVVRTLVPVLGGIDDRQRQAAASLGAGPLRTFATVDLPAMWRPLTAAAGFAFAVSLG
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHH
EFGATSFLAREDHPTLPIVIYHLIGHPGAMNYGMALAASVVLATTTALVMLAVDRLRVPS
HCCCHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VGAF
CCCC
>Mature Secondary Structure
MRRSLELVGLAALPLAVLGVFFLLPVSGMVSEGFVTDGRFDPGGVLEVLGRPRVHRVLWF
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHH
TVWSSTVATLLAVLLGLPVAHLLHRRRFWGRRALRSLLLVPFVLPTVVVGVAFRELIGEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GPLGFLGLDGSAVAIVAGLVFFNVAVVIRAVGAAWESLDPRPAEAAAALGAGPAHVFRTV
CCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHH
TLPALRPAIVSAASVVFLFCATAFGVVLTLGGLRYSSVETEIYLLTTNLLDLQAAAALSI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEECCHHHHHHHHHHHH
LQLLAITALLVVTGRLRAVPDPALARSTRRPPRPAPGDWPGLVATGLVLLLVGAPVAALV
HHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHH
AGSLRVGDAWSLGNYRALTGEGTQHALLASATDALVTSLRTAVDATWMSLSLGVLVTVVV
HCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TRRSRTRGERRARGLLDGLFMLPLGVSAVTLGFGFLITLDRPPLDLRDSPLLVPIAQALV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ALPLVVRTLVPVLGGIDDRQRQAAASLGAGPLRTFATVDLPAMWRPLTAAAGFAFAVSLG
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHH
EFGATSFLAREDHPTLPIVIYHLIGHPGAMNYGMALAASVVLATTTALVMLAVDRLRVPS
HCCCHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VGAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]