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The map label for this gene is ilvB [H]

Identifier: 119714308

GI number: 119714308

Start: 39830

End: 41461

Strand: Reverse

Name: ilvB [H]

Synonym: Noca_0036

Alternate gene names: 119714308

Gene position: 41461-39830 (Counterclockwise)

Preceding gene: 119714309

Following gene: 119714302

Centisome position: 0.83

GC content: 73.22

Gene sequence:

>1632_bases
ATGCCCCATGTAGGCGACGCGATGATCGACGCGATCGCGAGCGCAGGCGTCCGACGGCTCTACACCGTGCCCGGCGAGAG
CTTCTTGGAGGTGCTCGACGCCGCCGACCGGCACCCCGCGCTCCGCCAGTTCTCCACCCGGCACGAGTCGGGCGCCTCGT
TCATGGCCGACGCGGACGCCAAGACGTCCGGCGTGCCCGCCGTCGCGATGGCCACCCGGGGTCCGGGCGCGGCGAACCTC
AGCATCGGCGTGCACACCGCTCACCAGGACGGCACGCCCATGCTGGTTCTGATCGGGGACGTGGAGACGCCGCGGATCTT
CCGCGGCGCCTTCCAGGAGGTCGATCTGCCGGCGTTCTACCGGCCGATCACCAAGGCGGCCATGACCGCCCGCCGCGGGG
ACCGGCTGCCCGAGATGGTGACCGACGCGCTCCGGATCGCAGTCTCCGGACGCCCCGGCCCGGTCATGATCTCGCTGCCC
GCCGACCTGCTCGCGGAAGAGTTCACCGGGCCTGCGCCGGCGCCGCTCGCGCCCGTCGCGCCCGCCGGATGTCCCCCCGA
GACCGTCCGCCGGCTGCGGAGCACCCTGGAACGGGCGCAGCGACCGGTGGTCATCGCCGGCGCGGGCGTTCGCCACGCCA
CCGACCGCCTCGTCGCGCTCTGCGAGGCCTACGGTCTCGGCGTGTACGCCGCGATGCGACGGCAGGACGTCTTCCCCAAC
GACCACCCGCTCTACCTCGGCCACCTCGGTGTCTCGCCCGCGCCCGGCACCGTGGACGCGCTCCGCGAGGCCGACGTGGT
GCTGATCCTCGGCGCTGCGCTGGACCAGATGACGACCCAGCAGTTCACGCTGCCCGCCGCCTCGGCGCACCGGATCCACG
TCGACGCCGATCCGCTCACCCTCGGCGCCGGCCTGCCCGTCGACGAGGCTGTCGTCGCCGAGCCGCGCGAGCTGATCGAG
GCGCTGCTAGCCGAACCGCCCACCGCGCCGCACCGGGCCTGGGACGCCGCGCACCGACGGTTCCTGGAGTCGACATGCGC
CCCCCCGCGCACCCCGGCCCGGGGCTGCGACCCCGGTGCTGTGATCGAGGCCATGAAGCGCGCGTGGCCCGCCGACACGA
TCGTCACCAGCGACGCGGGCAACTTCTCGGGATTCCTCCACCAGTACTGGCGGTTCACGACCCCCCGCAGCCAGGCCGCG
CCCATCAACGGCGCGATGGGCTATGCCGTGCCAGGCGCCGTCGGCGCCAAGGCTGCCTCCCCCGACCGGCACGTGCTCGG
CGTGGTCGGCGACGGTGGCTTCCTGATGACCGGCAGCGAGGTCGAGACCGCCGTGCGCTACGGCCTGCCGCTGACGATCG
TCGTCCTGCGCAACGGGCTCTACGGCACGATCGCGATGCACCAGGCCCAGGAGATGGGCCGCCTGTCGGCCGTCGACATC
GGCGACGTGGACCTCGCGGCATACGGCCGCAGCCTCGGCGCGGAGGGCATCACGGTCGAGGAGCCGGGTCAGCTCGACGA
GGCCATGCGCACTGCCGCGACCAGCGATGCGGTCACCGTCGTCGACGTCGTCACCGACCCGGACCTCATCACCGCGTCCG
GCCGGCTCTCGGAGATGTTCCAGACCTCCTGA

Upstream 100 bases:

>100_bases
CTGGCCGCTCCCCGCCTCGTCGACGGGGCGCTGAGCACCGGTCCCAGCTGACGCGATCTTCCGCGCCGGCACCACCTTCG
AGGTCCCGAGGAGCACATTC

Downstream 100 bases:

>100_bases
GCCCGCGGCGACCCGGACCGGGTTTCAGTCTTCGTCCGCTGCCTGGAGCACCCGCCGGTGCGTGAGGTAGGCCGAGCGGG
TGCGCTCGTTGTGCGAGCGC

Product: thiamine pyrophosphate binding domain-containing protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MPHVGDAMIDAIASAGVRRLYTVPGESFLEVLDAADRHPALRQFSTRHESGASFMADADAKTSGVPAVAMATRGPGAANL
SIGVHTAHQDGTPMLVLIGDVETPRIFRGAFQEVDLPAFYRPITKAAMTARRGDRLPEMVTDALRIAVSGRPGPVMISLP
ADLLAEEFTGPAPAPLAPVAPAGCPPETVRRLRSTLERAQRPVVIAGAGVRHATDRLVALCEAYGLGVYAAMRRQDVFPN
DHPLYLGHLGVSPAPGTVDALREADVVLILGAALDQMTTQQFTLPAASAHRIHVDADPLTLGAGLPVDEAVVAEPRELIE
ALLAEPPTAPHRAWDAAHRRFLESTCAPPRTPARGCDPGAVIEAMKRAWPADTIVTSDAGNFSGFLHQYWRFTTPRSQAA
PINGAMGYAVPGAVGAKAASPDRHVLGVVGDGGFLMTGSEVETAVRYGLPLTIVVLRNGLYGTIAMHQAQEMGRLSAVDI
GDVDLAAYGRSLGAEGITVEEPGQLDEAMRTAATSDAVTVVDVVTDPDLITASGRLSEMFQTS

Sequences:

>Translated_543_residues
MPHVGDAMIDAIASAGVRRLYTVPGESFLEVLDAADRHPALRQFSTRHESGASFMADADAKTSGVPAVAMATRGPGAANL
SIGVHTAHQDGTPMLVLIGDVETPRIFRGAFQEVDLPAFYRPITKAAMTARRGDRLPEMVTDALRIAVSGRPGPVMISLP
ADLLAEEFTGPAPAPLAPVAPAGCPPETVRRLRSTLERAQRPVVIAGAGVRHATDRLVALCEAYGLGVYAAMRRQDVFPN
DHPLYLGHLGVSPAPGTVDALREADVVLILGAALDQMTTQQFTLPAASAHRIHVDADPLTLGAGLPVDEAVVAEPRELIE
ALLAEPPTAPHRAWDAAHRRFLESTCAPPRTPARGCDPGAVIEAMKRAWPADTIVTSDAGNFSGFLHQYWRFTTPRSQAA
PINGAMGYAVPGAVGAKAASPDRHVLGVVGDGGFLMTGSEVETAVRYGLPLTIVVLRNGLYGTIAMHQAQEMGRLSAVDI
GDVDLAAYGRSLGAEGITVEEPGQLDEAMRTAATSDAVTVVDVVTDPDLITASGRLSEMFQTS
>Mature_542_residues
PHVGDAMIDAIASAGVRRLYTVPGESFLEVLDAADRHPALRQFSTRHESGASFMADADAKTSGVPAVAMATRGPGAANLS
IGVHTAHQDGTPMLVLIGDVETPRIFRGAFQEVDLPAFYRPITKAAMTARRGDRLPEMVTDALRIAVSGRPGPVMISLPA
DLLAEEFTGPAPAPLAPVAPAGCPPETVRRLRSTLERAQRPVVIAGAGVRHATDRLVALCEAYGLGVYAAMRRQDVFPND
HPLYLGHLGVSPAPGTVDALREADVVLILGAALDQMTTQQFTLPAASAHRIHVDADPLTLGAGLPVDEAVVAEPRELIEA
LLAEPPTAPHRAWDAAHRRFLESTCAPPRTPARGCDPGAVIEAMKRAWPADTIVTSDAGNFSGFLHQYWRFTTPRSQAAP
INGAMGYAVPGAVGAKAASPDRHVLGVVGDGGFLMTGSEVETAVRYGLPLTIVVLRNGLYGTIAMHQAQEMGRLSAVDIG
DVDLAAYGRSLGAEGITVEEPGQLDEAMRTAATSDAVTVVDVVTDPDLITASGRLSEMFQTS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21361361, Length=560, Percent_Identity=25.7142857142857, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=24.5931283905967, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI87081685, Length=549, Percent_Identity=30.7832422586521, Blast_Score=238, Evalue=7e-64,
Organism=Escherichia coli, GI1790104, Length=512, Percent_Identity=32.6171875, Blast_Score=225, Evalue=5e-60,
Organism=Escherichia coli, GI1787096, Length=535, Percent_Identity=27.8504672897196, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786717, Length=551, Percent_Identity=27.0417422867514, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1788716, Length=571, Percent_Identity=25.9194395796848, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17542570, Length=582, Percent_Identity=24.5704467353952, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17531299, Length=477, Percent_Identity=26.6247379454927, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17531301, Length=477, Percent_Identity=26.6247379454927, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=556, Percent_Identity=26.4388489208633, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6320816, Length=516, Percent_Identity=25, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922626, Length=554, Percent_Identity=26.5342960288809, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57122; Mature: 56990

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHVGDAMIDAIASAGVRRLYTVPGESFLEVLDAADRHPALRQFSTRHESGASFMADADA
CCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCC
KTSGVPAVAMATRGPGAANLSIGVHTAHQDGTPMLVLIGDVETPRIFRGAFQEVDLPAFY
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHH
RPITKAAMTARRGDRLPEMVTDALRIAVSGRPGPVMISLPADLLAEEFTGPAPAPLAPVA
HHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCEEEECCHHHHHHHCCCCCCCCCCCCC
PAGCPPETVRRLRSTLERAQRPVVIAGAGVRHATDRLVALCEAYGLGVYAAMRRQDVFPN
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DHPLYLGHLGVSPAPGTVDALREADVVLILGAALDQMTTQQFTLPAASAHRIHVDADPLT
CCCEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHEECCCCCCCEEEECCCCEE
LGAGLPVDEAVVAEPRELIEALLAEPPTAPHRAWDAAHRRFLESTCAPPRTPARGCDPGA
ECCCCCCCHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VIEAMKRAWPADTIVTSDAGNFSGFLHQYWRFTTPRSQAAPINGAMGYAVPGAVGAKAAS
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDRHVLGVVGDGGFLMTGSEVETAVRYGLPLTIVVLRNGLYGTIAMHQAQEMGRLSAVDI
CCCEEEEEEECCCEEEECHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCEEECCC
GDVDLAAYGRSLGAEGITVEEPGQLDEAMRTAATSDAVTVVDVVTDPDLITASGRLSEMF
CCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
QTS
HCC
>Mature Secondary Structure 
PHVGDAMIDAIASAGVRRLYTVPGESFLEVLDAADRHPALRQFSTRHESGASFMADADA
CCCHHHHHHHHHHHCCCEEEECCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCC
KTSGVPAVAMATRGPGAANLSIGVHTAHQDGTPMLVLIGDVETPRIFRGAFQEVDLPAFY
CCCCCCEEEEEECCCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHH
RPITKAAMTARRGDRLPEMVTDALRIAVSGRPGPVMISLPADLLAEEFTGPAPAPLAPVA
HHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCCCEEEECCHHHHHHHCCCCCCCCCCCCC
PAGCPPETVRRLRSTLERAQRPVVIAGAGVRHATDRLVALCEAYGLGVYAAMRRQDVFPN
CCCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCC
DHPLYLGHLGVSPAPGTVDALREADVVLILGAALDQMTTQQFTLPAASAHRIHVDADPLT
CCCEEEEECCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHEECCCCCCCEEEECCCCEE
LGAGLPVDEAVVAEPRELIEALLAEPPTAPHRAWDAAHRRFLESTCAPPRTPARGCDPGA
ECCCCCCCHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VIEAMKRAWPADTIVTSDAGNFSGFLHQYWRFTTPRSQAAPINGAMGYAVPGAVGAKAAS
HHHHHHHHCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PDRHVLGVVGDGGFLMTGSEVETAVRYGLPLTIVVLRNGLYGTIAMHQAQEMGRLSAVDI
CCCEEEEEEECCCEEEECHHHHHHHHHCCCEEEEEECCCCEEEHHHHHHHHCCCEEECCC
GDVDLAAYGRSLGAEGITVEEPGQLDEAMRTAATSDAVTVVDVVTDPDLITASGRLSEMF
CCCHHHHHHHHCCCCCCEECCCCHHHHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
QTS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]