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The map label for this gene is gcvA [H]

Identifier: 119387118

GI number: 119387118

Start: 1613891

End: 1614793

Strand: Direct

Name: gcvA [H]

Synonym: Pden_4413

Alternate gene names: 119387118

Gene position: 1613891-1614793 (Clockwise)

Preceding gene: 119387115

Following gene: 119387120

Centisome position: 93.28

GC content: 68.11

Gene sequence:

>903_bases
TTGCGGCAAAGGCGCTTTCTTCCGAACCTCAGCCTGCTGCTGGCCTTCGACGCGGTGATGCGTACCGGCTCGGTCACCGG
CGCGGCGCAGGACCTGGGGCTGACGCAAAGCACGGTCAGCCGGCTGCTGCAAAGCCTGGAGGCGCAACTGGGGCAGGAAC
TGTTCATCCGCCACAAGAAGCGCCTGACCCCGACCGAGGCGGCCGAGCGGTATTTCCGCGATATTTCCGCCGCGCTGGAC
CTGATCCAGCGCGGCAGCATGTCGCTGATCGCCAACCCGGATGGAGGCTCACTGGATCTGGCGGTTCTGCCGACCTTTGC
CACGCGCTGGCTGGCGCCGCGGCTGCCGGATTTCCTGTCAGGGCATCCGGGCGTGTCGATCAACCTGACCACGCGCTTTC
GCCGCTTCAGCTTCGATGAGGAGCCCTTTGACGCGATGATCTTCTACGGCCGGGACGACTGGCCGGGGGCCGAGCATCTG
AAACTGTTCGATGAACGCCTCACCGCCTGCGCCGCACCCGATCTGCTGGCACGAAACCCGGTGGCAACGCCCGCCGATCT
GGCCGGGATGGTGCTGTTGCAGTTGCCGACCCGCCCCGATGCCTGGCAGGCATGGTTCCGGGGACAGGGCGCCGAGCCGC
CCGCGCCTCTGGACGGGATGATGATGGACCAGTTCTCGATGATGATCCAGGCGGCGATTTCGGGACTGGGGATCGCGCTC
CTGCCCGATTACCTGGCCCGGGTCGAGATCGCCGAGGGCCGGCTGGCGCCGGTGCTGCGCCAGGGCGTGCCGGGCAGCGG
CGCCTATTGGCTGGCCTGGCCGGAGCGCAAGGCCGGGCTGAAGCCGCTGGCCGCCTTTCGCGACTGGATCGCCGACCGCA
TGGCACGGGACGGCATGGGATAG

Upstream 100 bases:

>100_bases
ATGCCTCATGCGCTGATGCTTGTCGGATCATTCATTACCGCTTTCAAACCGGGCCGGAAAATGGATAATCCTCATTACTT
CATGCACGGGGTGAATGTTC

Downstream 100 bases:

>100_bases
CGGGCGGCCGGGGCCGTCCGCTTTCAGGCCGGGACCAGCGCCGCCTCGATGGCCGCTTCCAGGATCGCCAGCGCCTCGGA
CAATTGCTCGTCGGGGATGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRQRRFLPNLSLLLAFDAVMRTGSVTGAAQDLGLTQSTVSRLLQSLEAQLGQELFIRHKKRLTPTEAAERYFRDISAALD
LIQRGSMSLIANPDGGSLDLAVLPTFATRWLAPRLPDFLSGHPGVSINLTTRFRRFSFDEEPFDAMIFYGRDDWPGAEHL
KLFDERLTACAAPDLLARNPVATPADLAGMVLLQLPTRPDAWQAWFRGQGAEPPAPLDGMMMDQFSMMIQAAISGLGIAL
LPDYLARVEIAEGRLAPVLRQGVPGSGAYWLAWPERKAGLKPLAAFRDWIADRMARDGMG

Sequences:

>Translated_300_residues
MRQRRFLPNLSLLLAFDAVMRTGSVTGAAQDLGLTQSTVSRLLQSLEAQLGQELFIRHKKRLTPTEAAERYFRDISAALD
LIQRGSMSLIANPDGGSLDLAVLPTFATRWLAPRLPDFLSGHPGVSINLTTRFRRFSFDEEPFDAMIFYGRDDWPGAEHL
KLFDERLTACAAPDLLARNPVATPADLAGMVLLQLPTRPDAWQAWFRGQGAEPPAPLDGMMMDQFSMMIQAAISGLGIAL
LPDYLARVEIAEGRLAPVLRQGVPGSGAYWLAWPERKAGLKPLAAFRDWIADRMARDGMG
>Mature_300_residues
MRQRRFLPNLSLLLAFDAVMRTGSVTGAAQDLGLTQSTVSRLLQSLEAQLGQELFIRHKKRLTPTEAAERYFRDISAALD
LIQRGSMSLIANPDGGSLDLAVLPTFATRWLAPRLPDFLSGHPGVSINLTTRFRRFSFDEEPFDAMIFYGRDDWPGAEHL
KLFDERLTACAAPDLLARNPVATPADLAGMVLLQLPTRPDAWQAWFRGQGAEPPAPLDGMMMDQFSMMIQAAISGLGIAL
LPDYLARVEIAEGRLAPVLRQGVPGSGAYWLAWPERKAGLKPLAAFRDWIADRMARDGMG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=290, Percent_Identity=36.8965517241379, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=30.6620209059233, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=285, Percent_Identity=25.9649122807018, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=28.2758620689655, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=27.0547945205479, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=26.8211920529801, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790262, Length=244, Percent_Identity=27.4590163934426, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789639, Length=262, Percent_Identity=27.0992366412214, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=24.9134948096886, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=125, Percent_Identity=38.4, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33105; Mature: 33105

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQRRFLPNLSLLLAFDAVMRTGSVTGAAQDLGLTQSTVSRLLQSLEAQLGQELFIRHKK
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLTPTEAAERYFRDISAALDLIQRGSMSLIANPDGGSLDLAVLPTFATRWLAPRLPDFLS
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCCCHHHC
GHPGVSINLTTRFRRFSFDEEPFDAMIFYGRDDWPGAEHLKLFDERLTACAAPDLLARNP
CCCCEEEEEEHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHCCC
VATPADLAGMVLLQLPTRPDAWQAWFRGQGAEPPAPLDGMMMDQFSMMIQAAISGLGIAL
CCCCHHHHCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LPDYLARVEIAEGRLAPVLRQGVPGSGAYWLAWPERKAGLKPLAAFRDWIADRMARDGMG
HHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRQRRFLPNLSLLLAFDAVMRTGSVTGAAQDLGLTQSTVSRLLQSLEAQLGQELFIRHKK
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLTPTEAAERYFRDISAALDLIQRGSMSLIANPDGGSLDLAVLPTFATRWLAPRLPDFLS
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEHHHHHHHHHCCCCCHHHC
GHPGVSINLTTRFRRFSFDEEPFDAMIFYGRDDWPGAEHLKLFDERLTACAAPDLLARNP
CCCCEEEEEEHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHCCC
VATPADLAGMVLLQLPTRPDAWQAWFRGQGAEPPAPLDGMMMDQFSMMIQAAISGLGIAL
CCCCHHHHCEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHH
LPDYLARVEIAEGRLAPVLRQGVPGSGAYWLAWPERKAGLKPLAAFRDWIADRMARDGMG
HHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]