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The map label for this gene is dctA2 [H]

Identifier: 119387073

GI number: 119387073

Start: 1559113

End: 1560438

Strand: Direct

Name: dctA2 [H]

Synonym: Pden_4368

Alternate gene names: 119387073

Gene position: 1559113-1560438 (Clockwise)

Preceding gene: 119387070

Following gene: 119387075

Centisome position: 90.12

GC content: 65.91

Gene sequence:

>1326_bases
ATGCCGAATTGGTCCAGGTCCTTGTTTGGACAGGTTTGCGTGGCACTGGTGCTGGGGGTCGTCGCCGGCTTCTTCGCGCC
CGAGTTCGCCGCCAAGATGAAGCCGCTTGGGGACGGGTTCATCAAGCTCATCAAGATGCTGATCCCCGTCATCGTGTTCT
GCGTCGTGGTGCATGGCATCGCCGGCGCGGGCGACCTGAAAAAGGTCGGCAAGGTCGGCGTGCGCGCCATCATCTATTTC
GAGATCATCACCACCATCGCGCTGGCCCTGGGCATCGCGGTCGCCTATCTGTTCGGGCCGGGCCACGGCATGAACATCGA
CGTGAGCCAACTCGACGCCTCGGCGCTGTCGGCCTATGTCGAGCGCGCGGACCAGGTCACCAGCGGCGGCACCGTCGACT
TCCTGATGAAGCTGATCCCGACCACGATGATCAGCGGCTTCGCACAGGGCGACATCCTGCAGGTGCTGCTGGTCGCGATC
CTGTTCGGCTGCGCGCTGTCGCTGGTGGGCGAACGCGGCAAGCCGCTTCTGCACATGATCGAGCTGACGGGCGACGTCAC
CTTCAAGATCATGTTCTTCATCGTCCGGCTGGCGCCGCTGGGCGTGTTCGGCGCCATCGCCTTCACCGTCGGGCAATACG
GCATCGGCTCGCTGGCGCAGCTGGGCTATCTGGTGGGGCTGTTCTATGTCACCATCGCCGTGTTCGTCTTCGTGGTGCTG
GGCTTCGTCCTGCGCCTTGCGGGCTTCAGCATCTTCAAGCTGCTGCGCTATCTGCGCACCGAGGTCGGCATCGTCGCCGG
CACGGCCAGCAGCGACGCCGTGCTGCCGCAGACCATGCGCAAGCTGGAACACCTGGGCATCAAGGACTCGACCGTGGGGC
TGGTTGTGCCGACGGGCTATTCCTTCAACCTCGACGCCTTCTCGATCTACCTGACGCTGGCGGCGGTCTTCATCGCCCAG
GCCACCAACACGCCGCTGTCGACCACCGACCTGCTGGCCATCCTGGGCGTGGCGCTGGTGACCTCGAAGGGCGCGCATGG
CGTACCGGGCTCGGCCATCGTGATCCTGGCCGCGACGCTGGCCGCGATCCCGGCGATCCCCGCCATTGGGCTGGTCCTGG
TGCTGTCGGTCGACTGGTTCATGGGCATCGCCCGGGCGCTGGGGAACTATCTGGGCAATTGCGTCGCCACCGTGGTCGTC
GCCTCCTGGGTGGGCGACATCGACCGCGACCGCGCCCGCCGCGTCCTGGAAGGCGAGGACATGCCGACGCTGGACGTGCT
GGACGAGGATACGCCGATGTCCGAGCCCGAGGCCCAGCACGGTTGA

Upstream 100 bases:

>100_bases
GGGGTCAGCCGCGACCGCGCGCCATCGATGCGAAGGGAGACGCATCCGAAGGCGCCGCGCCGCAGCCTGCCCCTTCCGTC
ACAGGGAGGAACGGAAACAC

Downstream 100 bases:

>100_bases
CGAAGTCCCTCGCGGAGTGACCGGGACCAGCGCGACGGACCCGGTCGCCCCGCCGAGAAGACGGCCTGCGCCTTCCCTCC
CGATGGCGCAGGCCTTTTCG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MPNWSRSLFGQVCVALVLGVVAGFFAPEFAAKMKPLGDGFIKLIKMLIPVIVFCVVVHGIAGAGDLKKVGKVGVRAIIYF
EIITTIALALGIAVAYLFGPGHGMNIDVSQLDASALSAYVERADQVTSGGTVDFLMKLIPTTMISGFAQGDILQVLLVAI
LFGCALSLVGERGKPLLHMIELTGDVTFKIMFFIVRLAPLGVFGAIAFTVGQYGIGSLAQLGYLVGLFYVTIAVFVFVVL
GFVLRLAGFSIFKLLRYLRTEVGIVAGTASSDAVLPQTMRKLEHLGIKDSTVGLVVPTGYSFNLDAFSIYLTLAAVFIAQ
ATNTPLSTTDLLAILGVALVTSKGAHGVPGSAIVILAATLAAIPAIPAIGLVLVLSVDWFMGIARALGNYLGNCVATVVV
ASWVGDIDRDRARRVLEGEDMPTLDVLDEDTPMSEPEAQHG

Sequences:

>Translated_441_residues
MPNWSRSLFGQVCVALVLGVVAGFFAPEFAAKMKPLGDGFIKLIKMLIPVIVFCVVVHGIAGAGDLKKVGKVGVRAIIYF
EIITTIALALGIAVAYLFGPGHGMNIDVSQLDASALSAYVERADQVTSGGTVDFLMKLIPTTMISGFAQGDILQVLLVAI
LFGCALSLVGERGKPLLHMIELTGDVTFKIMFFIVRLAPLGVFGAIAFTVGQYGIGSLAQLGYLVGLFYVTIAVFVFVVL
GFVLRLAGFSIFKLLRYLRTEVGIVAGTASSDAVLPQTMRKLEHLGIKDSTVGLVVPTGYSFNLDAFSIYLTLAAVFIAQ
ATNTPLSTTDLLAILGVALVTSKGAHGVPGSAIVILAATLAAIPAIPAIGLVLVLSVDWFMGIARALGNYLGNCVATVVV
ASWVGDIDRDRARRVLEGEDMPTLDVLDEDTPMSEPEAQHG
>Mature_440_residues
PNWSRSLFGQVCVALVLGVVAGFFAPEFAAKMKPLGDGFIKLIKMLIPVIVFCVVVHGIAGAGDLKKVGKVGVRAIIYFE
IITTIALALGIAVAYLFGPGHGMNIDVSQLDASALSAYVERADQVTSGGTVDFLMKLIPTTMISGFAQGDILQVLLVAIL
FGCALSLVGERGKPLLHMIELTGDVTFKIMFFIVRLAPLGVFGAIAFTVGQYGIGSLAQLGYLVGLFYVTIAVFVFVVLG
FVLRLAGFSIFKLLRYLRTEVGIVAGTASSDAVLPQTMRKLEHLGIKDSTVGLVVPTGYSFNLDAFSIYLTLAAVFIAQA
TNTPLSTTDLLAILGVALVTSKGAHGVPGSAIVILAATLAAIPAIPAIGLVLVLSVDWFMGIARALGNYLGNCVATVVVA
SWVGDIDRDRARRVLEGEDMPTLDVLDEDTPMSEPEAQHG

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=463, Percent_Identity=25.7019438444924, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI4827012, Length=308, Percent_Identity=27.5974025974026, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI169790839, Length=482, Percent_Identity=23.4439834024896, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI21314632, Length=441, Percent_Identity=25.6235827664399, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI66773030, Length=509, Percent_Identity=25.147347740668, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI194239697, Length=477, Percent_Identity=22.6415094339623, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI223468564, Length=298, Percent_Identity=27.1812080536913, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI223468566, Length=298, Percent_Identity=27.1812080536913, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI5032093, Length=429, Percent_Identity=25.1748251748252, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI301601644, Length=206, Percent_Identity=27.1844660194175, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI262359914, Length=380, Percent_Identity=23.1578947368421, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789947, Length=414, Percent_Identity=51.207729468599, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1790514, Length=435, Percent_Identity=39.7701149425287, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1789473, Length=326, Percent_Identity=26.9938650306748, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788024, Length=401, Percent_Identity=23.6907730673317, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=393, Percent_Identity=26.4631043256997, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI193206654, Length=449, Percent_Identity=24.4988864142539, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI193206505, Length=457, Percent_Identity=24.0700218818381, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71983106, Length=467, Percent_Identity=24.1970021413276, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71983099, Length=467, Percent_Identity=24.1970021413276, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71996953, Length=424, Percent_Identity=24.0566037735849, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17541374, Length=447, Percent_Identity=22.5950782997763, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583025, Length=399, Percent_Identity=25.5639097744361, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17137668, Length=399, Percent_Identity=25.5639097744361, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24583023, Length=399, Percent_Identity=25.5639097744361, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281360481, Length=455, Percent_Identity=25.2747252747253, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI281360483, Length=455, Percent_Identity=25.2747252747253, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17137666, Length=455, Percent_Identity=25.2747252747253, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46480; Mature: 46349

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PNWSRSLFGQVCVALVLGVVAGFFAPEFAAKMKPLGDGFIKLIKMLIPVIVFCVVVHGI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
AGAGDLKKVGKVGVRAIIYFEIITTIALALGIAVAYLFGPGHGMNIDVSQLDASALSAYV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
ERADQVTSGGTVDFLMKLIPTTMISGFAQGDILQVLLVAILFGCALSLVGERGKPLLHMI
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ELTGDVTFKIMFFIVRLAPLGVFGAIAFTVGQYGIGSLAQLGYLVGLFYVTIAVFVFVVL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GFVLRLAGFSIFKLLRYLRTEVGIVAGTASSDAVLPQTMRKLEHLGIKDSTVGLVVPTGY
HHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEECCC
SFNLDAFSIYLTLAAVFIAQATNTPLSTTDLLAILGVALVTSKGAHGVPGSAIVILAATL
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
AAIPAIPAIGLVLVLSVDWFMGIARALGNYLGNCVATVVVASWVGDIDRDRARRVLEGED
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
MPTLDVLDEDTPMSEPEAQHG
CCCHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]