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The map label for this gene is hupR1 [H]

Identifier: 119385831

GI number: 119385831

Start: 292889

End: 294370

Strand: Direct

Name: hupR1 [H]

Synonym: Pden_3109

Alternate gene names: 119385831

Gene position: 292889-294370 (Clockwise)

Preceding gene: 119385830

Following gene: 119385832

Centisome position: 16.93

GC content: 66.4

Gene sequence:

>1482_bases
ATGCCCACGCCGCGCCCCTTTGTCCTGCTGGTGGATGACGAGCCGCATTCGCTTTCGGCGATGCGCATGGCGCTTGAGGA
TGAGTTTGAAATCCTGACCGCCCCGGATGCCGCCGCCGCCGCCCGGCTGATGGAAGAGGAATGGGTGCAGGTAGTGGTCT
GCGACCAGCGGATGCCCGGCCAGACCGGGGTGGAGTTCCTGACCGGGCTGCGCGAGCGCTGGCCGGAAACGGTGCGGCTT
CTGATAACCGGCTATACCGATCCCTCGGCCATCGTCGATGCGATCAATCTTGCCGGCATCCATCAGTTCATCGCCAAGCC
CTGGCATCCCGACCAGTTGCTGATGGCGGTGCGCAATGCGGCGCAGCTTTTCCATCTTGCGCGCGAGAATGAACGTCTGG
CGCTGGAGATGCGCCGGCTTGCCTCGACCTCCGAAACCCGGCTGGAAAAGCGCCGGCGGGCGTTGCGTCAGGGGCAGGGC
TTCGACAGCGTGCTGCGTGCGCCCAACTCGCCCATGAACGCCACGGTTGATGCGGCGCGGCATTATGCGGCCTTCGATGT
GCCGGTGCTGCTGACGGGCGAGCAGGGCACCGGCAAGGAGCAGCTTGCGCAGGCGATGCATCTGGCCTCGCTGAGGGCGG
ACCGGCCCTTTTACGCGCTGGATCTGACCGGAATGCCCGATGAGCTGGTCATGGTCGAGCTGCTGGGCGCGCGGCGCGGG
GCGGTGGCCGGGGGAACCAGCCGGATCGGCCTGATTCAAAAGGCGGATCGCGGGACGCTGTATCTGTCGGGGATCGAGAC
CATCACGCCCGAATTGCAGCTTGTGCTTTTGCGCATCCTGTCCGGGCGCGGCTTCACGCCGGTCGGCGGGCAAGAGACGC
TGACCAGCAATCTGCGCCTGATCGCCGGCGCCGGCACGGATTTGGCGGCTCTGGTCACCGAAGGGCGTTTTCGCGCCGAC
CTCTATTACGCTCTGGCGGTGGCCGAAATTGCGGTGCCGCCCCTGCGCGCTCGGCGCGGCGATATCGCCATGCTGGCCCA
GCACATGCTGGCCGAGCTGGCGGCCCGTCATGGCAAGCTGGTGCATGGTTTTGACGGGCCCGCGCTGGAATTTCTGGAAA
GTTACGACTGGCCCGGCAATCTGCGCGAATTGTCGAACGAGATCACCCGGATGCTGATCCTTGCGCAAGACAGCGTTCTG
GGCGCCGATCTGATCTCTCGCCCGATCCTGCAGGGTCCGCCGGGTGAGGATGGCGCCGGCCGTCTGACCCAGGCGGTGCT
TGCCGGCGTCGGCAGCCTGAAAGAGCGGGTCGAACTGATCGAAATGCGTATCCTGCGCGAGACGCTGACCCGCCATCGCT
GGAACAAAAGCCGCGCGGCGGCCGAGCTGGGTGTGTCGCGGGTGGGCCTGCGCGCCAAGCTGGACCGCTATGGCGTCAAG
GACCCGGCCGAGCGTCAGGCCACCCCGGAAGAGGAGGAATAG

Upstream 100 bases:

>100_bases
CCCCGGCATAGACATCCTGCGCCTTTCGGCCCGCACCGGCGAGGGGATGCAGGCCTGGATCGACTGGCTGCGTGCCGGTC
TGGCGACAAAGTCCTGAGCC

Downstream 100 bases:

>100_bases
CCATGTGTCTGGGAATTCCCGGTCGCATCATCGCCATGACCGATCCCGCGCGGATGATGGCCATGGCCGAGGTCTCGGGC
GTGCGCCGCGAGGTCAATAT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MPTPRPFVLLVDDEPHSLSAMRMALEDEFEILTAPDAAAAARLMEEEWVQVVVCDQRMPGQTGVEFLTGLRERWPETVRL
LITGYTDPSAIVDAINLAGIHQFIAKPWHPDQLLMAVRNAAQLFHLARENERLALEMRRLASTSETRLEKRRRALRQGQG
FDSVLRAPNSPMNATVDAARHYAAFDVPVLLTGEQGTGKEQLAQAMHLASLRADRPFYALDLTGMPDELVMVELLGARRG
AVAGGTSRIGLIQKADRGTLYLSGIETITPELQLVLLRILSGRGFTPVGGQETLTSNLRLIAGAGTDLAALVTEGRFRAD
LYYALAVAEIAVPPLRARRGDIAMLAQHMLAELAARHGKLVHGFDGPALEFLESYDWPGNLRELSNEITRMLILAQDSVL
GADLISRPILQGPPGEDGAGRLTQAVLAGVGSLKERVELIEMRILRETLTRHRWNKSRAAAELGVSRVGLRAKLDRYGVK
DPAERQATPEEEE

Sequences:

>Translated_493_residues
MPTPRPFVLLVDDEPHSLSAMRMALEDEFEILTAPDAAAAARLMEEEWVQVVVCDQRMPGQTGVEFLTGLRERWPETVRL
LITGYTDPSAIVDAINLAGIHQFIAKPWHPDQLLMAVRNAAQLFHLARENERLALEMRRLASTSETRLEKRRRALRQGQG
FDSVLRAPNSPMNATVDAARHYAAFDVPVLLTGEQGTGKEQLAQAMHLASLRADRPFYALDLTGMPDELVMVELLGARRG
AVAGGTSRIGLIQKADRGTLYLSGIETITPELQLVLLRILSGRGFTPVGGQETLTSNLRLIAGAGTDLAALVTEGRFRAD
LYYALAVAEIAVPPLRARRGDIAMLAQHMLAELAARHGKLVHGFDGPALEFLESYDWPGNLRELSNEITRMLILAQDSVL
GADLISRPILQGPPGEDGAGRLTQAVLAGVGSLKERVELIEMRILRETLTRHRWNKSRAAAELGVSRVGLRAKLDRYGVK
DPAERQATPEEEE
>Mature_492_residues
PTPRPFVLLVDDEPHSLSAMRMALEDEFEILTAPDAAAAARLMEEEWVQVVVCDQRMPGQTGVEFLTGLRERWPETVRLL
ITGYTDPSAIVDAINLAGIHQFIAKPWHPDQLLMAVRNAAQLFHLARENERLALEMRRLASTSETRLEKRRRALRQGQGF
DSVLRAPNSPMNATVDAARHYAAFDVPVLLTGEQGTGKEQLAQAMHLASLRADRPFYALDLTGMPDELVMVELLGARRGA
VAGGTSRIGLIQKADRGTLYLSGIETITPELQLVLLRILSGRGFTPVGGQETLTSNLRLIAGAGTDLAALVTEGRFRADL
YYALAVAEIAVPPLRARRGDIAMLAQHMLAELAARHGKLVHGFDGPALEFLESYDWPGNLRELSNEITRMLILAQDSVLG
ADLISRPILQGPPGEDGAGRLTQAVLAGVGSLKERVELIEMRILRETLTRHRWNKSRAAAELGVSRVGLRAKLDRYGVKD
PAERQATPEEEE

Specific function: Probable member of the two-component regulatory system involved in the regulation of the [NiFe] hydrogenase activity. HupR1 is probably phosphorylated by the sensory component and then acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=479, Percent_Identity=33.8204592901879, Blast_Score=243, Evalue=3e-65,
Organism=Escherichia coli, GI1788905, Length=473, Percent_Identity=31.7124735729387, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1790437, Length=471, Percent_Identity=31.4225053078556, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789233, Length=293, Percent_Identity=34.4709897610922, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1790299, Length=401, Percent_Identity=32.1695760598504, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=34.984520123839, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87082117, Length=304, Percent_Identity=36.1842105263158, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1789087, Length=315, Percent_Identity=34.9206349206349, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI87082152, Length=306, Percent_Identity=33.9869281045752, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787583, Length=320, Percent_Identity=32.1875, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786524, Length=308, Percent_Identity=31.1688311688312, Blast_Score=122, Evalue=6e-29,
Organism=Escherichia coli, GI87081858, Length=219, Percent_Identity=37.4429223744292, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789828, Length=326, Percent_Identity=30.6748466257669, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54190; Mature: 54059

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPRPFVLLVDDEPHSLSAMRMALEDEFEILTAPDAAAAARLMEEEWVQVVVCDQRMPG
CCCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEEEEECCCCCC
QTGVEFLTGLRERWPETVRLLITGYTDPSAIVDAINLAGIHQFIAKPWHPDQLLMAVRNA
CHHHHHHHHHHHHCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQLFHLARENERLALEMRRLASTSETRLEKRRRALRQGQGFDSVLRAPNSPMNATVDAAR
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHH
HYAAFDVPVLLTGEQGTGKEQLAQAMHLASLRADRPFYALDLTGMPDELVMVELLGARRG
HHHEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
AVAGGTSRIGLIQKADRGTLYLSGIETITPELQLVLLRILSGRGFTPVGGQETLTSNLRL
CCCCCCCCCCEEEECCCCEEEEECHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEE
IAGAGTDLAALVTEGRFRADLYYALAVAEIAVPPLRARRGDIAMLAQHMLAELAARHGKL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHCCCE
VHGFDGPALEFLESYDWPGNLRELSNEITRMLILAQDSVLGADLISRPILQGPPGEDGAG
EECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHH
RLTQAVLAGVGSLKERVELIEMRILRETLTRHRWNKSRAAAELGVSRVGLRAKLDRYGVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPAERQATPEEEE
CCHHHCCCCCCCC
>Mature Secondary Structure 
PTPRPFVLLVDDEPHSLSAMRMALEDEFEILTAPDAAAAARLMEEEWVQVVVCDQRMPG
CCCCCEEEEECCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHEEEEECCCCCC
QTGVEFLTGLRERWPETVRLLITGYTDPSAIVDAINLAGIHQFIAKPWHPDQLLMAVRNA
CHHHHHHHHHHHHCHHEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AQLFHLARENERLALEMRRLASTSETRLEKRRRALRQGQGFDSVLRAPNSPMNATVDAAR
HHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHH
HYAAFDVPVLLTGEQGTGKEQLAQAMHLASLRADRPFYALDLTGMPDELVMVELLGARRG
HHHEECCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCC
AVAGGTSRIGLIQKADRGTLYLSGIETITPELQLVLLRILSGRGFTPVGGQETLTSNLRL
CCCCCCCCCCEEEECCCCEEEEECHHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHCCCEE
IAGAGTDLAALVTEGRFRADLYYALAVAEIAVPPLRARRGDIAMLAQHMLAELAARHGKL
EEECCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHCCCE
VHGFDGPALEFLESYDWPGNLRELSNEITRMLILAQDSVLGADLISRPILQGPPGEDGAG
EECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCHH
RLTQAVLAGVGSLKERVELIEMRILRETLTRHRWNKSRAAAELGVSRVGLRAKLDRYGVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCC
DPAERQATPEEEE
CCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885559; 8497190; 2007559 [H]