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The map label for this gene is rne [H]

Identifier: 119385690

GI number: 119385690

Start: 135504

End: 138113

Strand: Direct

Name: rne [H]

Synonym: Pden_2965

Alternate gene names: 119385690

Gene position: 135504-138113 (Clockwise)

Preceding gene: 119385689

Following gene: 119385691

Centisome position: 7.83

GC content: 68.31

Gene sequence:

>2610_bases
ATGTCAAAGAAAATGCTGATCGACGCCACGCATTCCGAGGAGACTCGGGTTGTCGTGGTCGATGGAACCAAGGTCGAGGA
ATTTGATTTCGAGACGGTCAACAAGCGGCAGCTTGCTGGCAACATCTATCTGGCCAAGGTAACGCGGGTCGAACCCTCGC
TCCAGGCCGCTTTCGTGGATTACGGCGGCAACCGCCATGGCTTCCTGGCCTTTGCCGAGATCCATCCCGATTACTACCAG
ATCCCCGCCGCCGACCGTAAGGCGCTGCTGGAAGAGGAGCGCCAGGCCGCCGCGGCCGAGGCCGACGAAGGCGAGAAGCC
CAAGCGCAACCCGCGCCGCCGCAAGCCGGCCAAGGCCGCCGCCGCCGAAAGCGGCGATGCCGTCCTGGTCTCGGACGAGA
TCGCCGGGATGACCGTGGTGGACCCCTCGGAGGGCGAGGCCGTGCCGCTGGACGCCCCGCTTGCCGCTGATACGGCCGAA
GCCGCGGCGCCGGCAGCGGCTGCGGACAGCGCCGAGGATGCCCCGGCCGAGGAAGCCGCCGAGGACGACGGCATCGAATC
CGTCGCCGAGGAGGACATGGCCGAGGAGATCTCGGCCCCCAAGAAGCCGCGCGCCCGCCGCTACAAGATCCAGGAGGTGA
TCAAGGTCCGGCAGATCATGCTGGTCCAGGTCGTCAAGGAGGAACGCGGCAACAAGGGCGCCGCGCTGACCACCTATCTG
TCGCTGGCCGGCCGCTATTGCGTGCTGATGCCGAACACGGCGCGCGGCGGCGGGATTTCCCGCAAGATCACCAACGCCGC
CGACCGCAAGAAGCTGAAGGAGATCGCCAGCGAACTGGAGGTGCCGGAAGGCGCCGGGCTGATCATCCGCACCGCCGGCA
GCCAGCGCACCCGCACCGAGATCCGCCGCGACTACGAATACCTGATGCGGCTGTGGGAGCAGATCCGCGATCTGACCTTC
AAGTCCATCGCCCCGGCGCCGATCTATGAGGAAGGCGACCTGATCAAGCGCACGATCCGCGATCTCTACAGCAAGGAGAT
CGACGAGGTCCTGGTCGAGGGCGAGCGCGGCTATCGCACGGCCAAGGACTTCATGAAGATGATCATGCCGTCCCACGCCA
AGAACGTGAAGCACTACACCGACGCGATGCCGCTTTACGCGCGCTATCAGGTGGAAAGCTATCTTGGCGGCATGTTCAAC
CCGGTCGTGCAGCTGAAATCCGGCGGCTATATCGTCATCGGCGTGACCGAGGCGCTGGTCGCCATCGACGTGAACTCGGG
CCGGGCCACCAAGGAAGGCTCGATCGAGGACACCGCGCTCAAGACCAACCTGGAAGCGGCCGAGGAGATCGCGCGCCAGC
TGCGGCTGCGCGACCTGGCCGGGCTGATCGTCATCGACTTCATCGACATGGAGGAGCGCAAGAACAACGCCGCCGTCGAG
AAGCGCTTCAAGGACAAGCTCAAGACCGACCGGGCGCGGATCCAGGTCGGCCGCATCTCGGGCTTCGGCCTGATGGAGAT
GTCGCGCCAGCGCCTGCGGCCGGGCATGCTGGAATCGACCACGCAGCCCTGCCCGCATTGCCATGGCACCGGGCTGATCC
GCAGCGACGACAGCCTGGCGCTGACCATCCTGCGCGCCATCGAGGAAGAGGGCACCCGCAAGCGTTCGCGCGAGGTTCTG
GTCAAGGCGCCGGTCGCGGTGGCCAACTTCCTGATGAACGCCAAGCGCGAGCACATCGCCAATATCGAGGCACGCTATGG
GCTTTCGGTCCGGGTCGAGGCCGATCCGGCGCTGATCTCGCCCGATTACGCCATCGAGAAGTTCAAGACCGCGACCCGCA
ACGTGCCCGAGGTGGTTTCGCCCGTGGTTTCGGTCGATGCCCGCCTGATGGCGCAGATCGACGACGAGGAGCCGGCCGAG
GAAGAGGTCGAAGAGATCGCCGACGAGCCGGTCGAGGAGGATCGCGCCGAGAGCGGGGATGGCGAGAATGGCAGCAAGCG
CCGCCGTCGCCGTCGTCGTCGCCGGGGCGGCAAGAATGGCGAGGACGAAGCGCCTCATGCCGGGGACGATGCCGATGAGG
TGGCCTTGGAAACCGAGGAGGCCGACCAGCCTGCCGAGGTCGAGGTGATCGTGGAGGCGGTCGAGAGCGCGCCCGAGCCG
GTGGCGGAGGAGGCCGAAAAGCCGCGCCGCCGCACCCGCACGCGCCGCCGCAAGGCGGATGAGCCGGCCGAAGCCGTGGC
TGAGGCGGGGCCTGCCGAGGGGGCCCAGCCCGGAGAACCGGCCGAGACCGCCGCAGACGCGCCACAGCCTGTTGCCGAGG
CTTCGGAGGTCGAGCAACCTGCCGAGGCCGTCGAACCCGCCGTCGAGCCGGAACCGGAGGCCGCCGCCGCGGATGTGCCG
CCTGCCGACGAGGCTGTGGAGACCGTGCCGGCCGGCGAAACCGCCGGATCCGAGGCCGTTGCGCCCCAGTCCGAGGCCGA
CACGGCCGCGGAGCCCGCGCCTGCGCCGGAGCCCGAGGCCTTGCCCGCCGCAGTCCGGGTGCCGGAGCCCGCGGCGGCGA
ACGAGGAACCCGCGCGTCCCAAGCGCCGCGGCTGGTGGTCCCTGGGCTGA

Upstream 100 bases:

>100_bases
ATTGCTGTTGGCGGTGCGGCCTTCGATGTCCTCTCATGCGGCAGCCGGACCTTGCCCCCGCTAATCTGCGGGCCGGCTCG
GCTGCCCGAAAGACGGAAAC

Downstream 100 bases:

>100_bases
TCTGTCGCAGCCTTCCACGAAAGAGTGACGGGCGCGGGGCTTTCCCGCGCCCTTTCCTTGCGCCAAGGTGCCCGACATGT
TGGGAATCGAGCTTGCAGAT

Product: ribonuclease

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 869; Mature: 868

Protein sequence:

>869_residues
MSKKMLIDATHSEETRVVVVDGTKVEEFDFETVNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFAEIHPDYYQ
IPAADRKALLEEERQAAAAEADEGEKPKRNPRRRKPAKAAAAESGDAVLVSDEIAGMTVVDPSEGEAVPLDAPLAADTAE
AAAPAAAADSAEDAPAEEAAEDDGIESVAEEDMAEEISAPKKPRARRYKIQEVIKVRQIMLVQVVKEERGNKGAALTTYL
SLAGRYCVLMPNTARGGGISRKITNAADRKKLKEIASELEVPEGAGLIIRTAGSQRTRTEIRRDYEYLMRLWEQIRDLTF
KSIAPAPIYEEGDLIKRTIRDLYSKEIDEVLVEGERGYRTAKDFMKMIMPSHAKNVKHYTDAMPLYARYQVESYLGGMFN
PVVQLKSGGYIVIGVTEALVAIDVNSGRATKEGSIEDTALKTNLEAAEEIARQLRLRDLAGLIVIDFIDMEERKNNAAVE
KRFKDKLKTDRARIQVGRISGFGLMEMSRQRLRPGMLESTTQPCPHCHGTGLIRSDDSLALTILRAIEEEGTRKRSREVL
VKAPVAVANFLMNAKREHIANIEARYGLSVRVEADPALISPDYAIEKFKTATRNVPEVVSPVVSVDARLMAQIDDEEPAE
EEVEEIADEPVEEDRAESGDGENGSKRRRRRRRRRGGKNGEDEAPHAGDDADEVALETEEADQPAEVEVIVEAVESAPEP
VAEEAEKPRRRTRTRRRKADEPAEAVAEAGPAEGAQPGEPAETAADAPQPVAEASEVEQPAEAVEPAVEPEPEAAAADVP
PADEAVETVPAGETAGSEAVAPQSEADTAAEPAPAPEPEALPAAVRVPEPAAANEEPARPKRRGWWSLG

Sequences:

>Translated_869_residues
MSKKMLIDATHSEETRVVVVDGTKVEEFDFETVNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFAEIHPDYYQ
IPAADRKALLEEERQAAAAEADEGEKPKRNPRRRKPAKAAAAESGDAVLVSDEIAGMTVVDPSEGEAVPLDAPLAADTAE
AAAPAAAADSAEDAPAEEAAEDDGIESVAEEDMAEEISAPKKPRARRYKIQEVIKVRQIMLVQVVKEERGNKGAALTTYL
SLAGRYCVLMPNTARGGGISRKITNAADRKKLKEIASELEVPEGAGLIIRTAGSQRTRTEIRRDYEYLMRLWEQIRDLTF
KSIAPAPIYEEGDLIKRTIRDLYSKEIDEVLVEGERGYRTAKDFMKMIMPSHAKNVKHYTDAMPLYARYQVESYLGGMFN
PVVQLKSGGYIVIGVTEALVAIDVNSGRATKEGSIEDTALKTNLEAAEEIARQLRLRDLAGLIVIDFIDMEERKNNAAVE
KRFKDKLKTDRARIQVGRISGFGLMEMSRQRLRPGMLESTTQPCPHCHGTGLIRSDDSLALTILRAIEEEGTRKRSREVL
VKAPVAVANFLMNAKREHIANIEARYGLSVRVEADPALISPDYAIEKFKTATRNVPEVVSPVVSVDARLMAQIDDEEPAE
EEVEEIADEPVEEDRAESGDGENGSKRRRRRRRRRGGKNGEDEAPHAGDDADEVALETEEADQPAEVEVIVEAVESAPEP
VAEEAEKPRRRTRTRRRKADEPAEAVAEAGPAEGAQPGEPAETAADAPQPVAEASEVEQPAEAVEPAVEPEPEAAAADVP
PADEAVETVPAGETAGSEAVAPQSEADTAAEPAPAPEPEALPAAVRVPEPAAANEEPARPKRRGWWSLG
>Mature_868_residues
SKKMLIDATHSEETRVVVVDGTKVEEFDFETVNKRQLAGNIYLAKVTRVEPSLQAAFVDYGGNRHGFLAFAEIHPDYYQI
PAADRKALLEEERQAAAAEADEGEKPKRNPRRRKPAKAAAAESGDAVLVSDEIAGMTVVDPSEGEAVPLDAPLAADTAEA
AAPAAAADSAEDAPAEEAAEDDGIESVAEEDMAEEISAPKKPRARRYKIQEVIKVRQIMLVQVVKEERGNKGAALTTYLS
LAGRYCVLMPNTARGGGISRKITNAADRKKLKEIASELEVPEGAGLIIRTAGSQRTRTEIRRDYEYLMRLWEQIRDLTFK
SIAPAPIYEEGDLIKRTIRDLYSKEIDEVLVEGERGYRTAKDFMKMIMPSHAKNVKHYTDAMPLYARYQVESYLGGMFNP
VVQLKSGGYIVIGVTEALVAIDVNSGRATKEGSIEDTALKTNLEAAEEIARQLRLRDLAGLIVIDFIDMEERKNNAAVEK
RFKDKLKTDRARIQVGRISGFGLMEMSRQRLRPGMLESTTQPCPHCHGTGLIRSDDSLALTILRAIEEEGTRKRSREVLV
KAPVAVANFLMNAKREHIANIEARYGLSVRVEADPALISPDYAIEKFKTATRNVPEVVSPVVSVDARLMAQIDDEEPAEE
EVEEIADEPVEEDRAESGDGENGSKRRRRRRRRRGGKNGEDEAPHAGDDADEVALETEEADQPAEVEVIVEAVESAPEPV
AEEAEKPRRRTRTRRRKADEPAEAVAEAGPAEGAQPGEPAETAADAPQPVAEASEVEQPAEAVEPAVEPEPEAAAADVPP
ADEAVETVPAGETAGSEAVAPQSEADTAAEPAPAPEPEALPAAVRVPEPAAANEEPARPKRRGWWSLG

Specific function: Matures 5S rRNA from its precursors from all the rRNA genes. Major endoribonuclease participating in mRNA turnover. It initiates the decay of RNAs by cutting them internally near their 5'-end. It is able to remove poly(A) tails by an endonucleolytic proce

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1787325, Length=445, Percent_Identity=44.7191011235955, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI87082235, Length=399, Percent_Identity=34.3358395989975, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR021968
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF12111 PNPase_C; PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 94781; Mature: 94650

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKMLIDATHSEETRVVVVDGTKVEEFDFETVNKRQLAGNIYLAKVTRVEPSLQAAFVD
CCCCEEEECCCCCCCEEEEEECCEEECCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
YGGNRHGFLAFAEIHPDYYQIPAADRKALLEEERQAAAAEADEGEKPKRNPRRRKPAKAA
CCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
AAESGDAVLVSDEIAGMTVVDPSEGEAVPLDAPLAADTAEAAAPAAAADSAEDAPAEEAA
HCCCCCEEEEECCCCCEEEECCCCCCEECCCCCCCCCCHHHHCCCHHCCCCCCCCHHHHH
EDDGIESVAEEDMAEEISAPKKPRARRYKIQEVIKVRQIMLVQVVKEERGNKGAALTTYL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLAGRYCVLMPNTARGGGISRKITNAADRKKLKEIASELEVPEGAGLIIRTAGSQRTRTE
HHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
IRRDYEYLMRLWEQIRDLTFKSIAPAPIYEEGDLIKRTIRDLYSKEIDEVLVEGERGYRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AKDFMKMIMPSHAKNVKHYTDAMPLYARYQVESYLGGMFNPVVQLKSGGYIVIGVTEALV
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHEEECCCCEEEEEECCEEE
AIDVNSGRATKEGSIEDTALKTNLEAAEEIARQLRLRDLAGLIVIDFIDMEERKNNAAVE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCCHHHHH
KRFKDKLKTDRARIQVGRISGFGLMEMSRQRLRPGMLESTTQPCPHCHGTGLIRSDDSLA
HHHHHHHHCCHHHEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEECCCHHH
LTILRAIEEEGTRKRSREVLVKAPVAVANFLMNAKREHIANIEARYGLSVRVEADPALIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEEC
PDYAIEKFKTATRNVPEVVSPVVSVDARLMAQIDDEEPAEEEVEEIADEPVEEDRAESGD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCC
GENGSKRRRRRRRRRGGKNGEDEAPHAGDDADEVALETEEADQPAEVEVIVEAVESAPEP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHCCCCH
VAEEAEKPRRRTRTRRRKADEPAEAVAEAGPAEGAQPGEPAETAADAPQPVAEASEVEQP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHCCC
AEAVEPAVEPEPEAAAADVPPADEAVETVPAGETAGSEAVAPQSEADTAAEPAPAPEPEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LPAAVRVPEPAAANEEPARPKRRGWWSLG
CCCEEECCCCCCCCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SKKMLIDATHSEETRVVVVDGTKVEEFDFETVNKRQLAGNIYLAKVTRVEPSLQAAFVD
CCCEEEECCCCCCCEEEEEECCEEECCCCHHHHHHHHCCCEEEEEEEECCCCHHHHHHH
YGGNRHGFLAFAEIHPDYYQIPAADRKALLEEERQAAAAEADEGEKPKRNPRRRKPAKAA
CCCCCCCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHH
AAESGDAVLVSDEIAGMTVVDPSEGEAVPLDAPLAADTAEAAAPAAAADSAEDAPAEEAA
HCCCCCEEEEECCCCCEEEECCCCCCEECCCCCCCCCCHHHHCCCHHCCCCCCCCHHHHH
EDDGIESVAEEDMAEEISAPKKPRARRYKIQEVIKVRQIMLVQVVKEERGNKGAALTTYL
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
SLAGRYCVLMPNTARGGGISRKITNAADRKKLKEIASELEVPEGAGLIIRTAGSQRTRTE
HHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHH
IRRDYEYLMRLWEQIRDLTFKSIAPAPIYEEGDLIKRTIRDLYSKEIDEVLVEGERGYRT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AKDFMKMIMPSHAKNVKHYTDAMPLYARYQVESYLGGMFNPVVQLKSGGYIVIGVTEALV
HHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHEEECCCCEEEEEECCEEE
AIDVNSGRATKEGSIEDTALKTNLEAAEEIARQLRLRDLAGLIVIDFIDMEERKNNAAVE
EEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHCCHHHHH
KRFKDKLKTDRARIQVGRISGFGLMEMSRQRLRPGMLESTTQPCPHCHGTGLIRSDDSLA
HHHHHHHHCCHHHEEEECCCCCCHHHHHHHHCCCCHHHCCCCCCCCCCCCCEEECCCHHH
LTILRAIEEEGTRKRSREVLVKAPVAVANFLMNAKREHIANIEARYGLSVRVEADPALIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCEEC
PDYAIEKFKTATRNVPEVVSPVVSVDARLMAQIDDEEPAEEEVEEIADEPVEEDRAESGD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCCC
GENGSKRRRRRRRRRGGKNGEDEAPHAGDDADEVALETEEADQPAEVEVIVEAVESAPEP
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCCCHHHHHHHHHHHCCCCH
VAEEAEKPRRRTRTRRRKADEPAEAVAEAGPAEGAQPGEPAETAADAPQPVAEASEVEQP
HHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHCCC
AEAVEPAVEPEPEAAAADVPPADEAVETVPAGETAGSEAVAPQSEADTAAEPAPAPEPEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
LPAAVRVPEPAAANEEPARPKRRGWWSLG
CCCEEECCCCCCCCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]