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The map label for this gene is dctD [H]

Identifier: 119385689

GI number: 119385689

Start: 133728

End: 135053

Strand: Direct

Name: dctD [H]

Synonym: Pden_2964

Alternate gene names: 119385689

Gene position: 133728-135053 (Clockwise)

Preceding gene: 119385688

Following gene: 119385690

Centisome position: 7.73

GC content: 66.37

Gene sequence:

>1326_bases
ATGAGCCGCACTTTGAAGATAGCCGTCGTGGACGACGAACCGGACATGCGCGAATCGATCAGCCAGTGGCTGGCGCTTTC
CGGCTTCGACGCCGAGACCTTCGCCACCGCCGAAGAGGCGCTGAAGGCGATCGGCGCCGACTGGCCGGGGGTGGTGATCT
CGGACATCCGCATGCCCGGCATGGACGGGATGGCTTTCCTCAAGCGGCTGATGGGCATCGATTCGGGCCTGCCCGTCATC
ATGATCACCGGCCATGGCGACGTGCCGATGGCGGTCGAGGCGATGCGCATCGGCGCCATGGACTTCATGGAAAAGCCCTT
CAACCCCGAGCGGCTGACCGAGCTGGTCAAGAAGGCCACCCAGGCCCGGCGCATGACGCTGGACAACCGCGCCCTGCGCC
GCGACCTGTCCGAAGGCCAGCAGGTCATGTCCAAGCTGATCGGGTCGAGCCCAGTCATGGATCGGCTGCGCGAGGACATC
CTGGACCTGGGCCAGGCCGACGGCCATGTGCTGATCGACGGCGAGACCGGCACCGGCAAGACGCTGGTGGCGCACGCGCT
GCATGCCGTGGGGCCGCGTGCCTCGAAGAAATTCGTGCCGATATCCTGCGCCGCCTATAACGACGAGGTCCTGTCGGCCA
AGCTGTTCGGCCCGGTCGAGAACGGGCTGCCCGCGGTCGAGGAAGCCCGCGGCGGCACGCTTTGCCTTGAGGATATCGAG
GCGCTGTCCGATGCCTTGCAGGCGCGGCTCCTGGCCTTCATCGCCGAGCAGGGCAGCCCGGCCGAGACCCGGATCATCGC
CATTTCCAACGCCCGCGGCGAAGGGCGCCGGGCGGAGGATTCGCTGCGTCCCGACCTGTTCTATCGCCTGACCGCGCTGA
AGATCACCCTGCCGCCGCTGCGCTCGCGCGGCGAGGATATCCTGACGCTTTTCACCCGCATGACCGAGCAATTCGCCGAG
GAATACGGCTGCGAGCCGCCGCAGGTGACGGCGCAGGAGGCGGCGCAGCTGTTGCAGGCGCCCTGGCCGGGCAATGTGCG
CCAGCTCATCAACGTGGCCGAGCGGGCCGTGCTGCAGAACCGTCGCGGCTCGGGTTCGATCACCTCGCTGCTGATGGCGG
ATGGCGAGGATACGCAGGAGGCGACGACGACCGAGGGCAAGCCGCTCAAGGAATATGTCGAAAGCTTCGAAAAGATGTTG
ATCGACAATACGATGCGCCGCCACAAGGGCAGCATCGCCGCGGTCATGGACGAGCTTTGCCTGCCGCGCCGGACGCTGAA
CGAGAAGATGGCGAAATACGGGCTGAGCCGCAGCGATTACCTGTAG

Upstream 100 bases:

>100_bases
GGCAGGGGCGCCGTATTCGAACTTGAACTGCCGTTGCACGATCCCGGCCAGAAGGCGGGGCCGCGGCTGGCGGCGGAATG
ATGAAAAGAGGTAAGGGCCC

Downstream 100 bases:

>100_bases
GCGGTCGGGGCAGGCGGCGCCCCGGGTCGGCAAATCCCGGCTCTCTGACTAAAAACAGATTGTAACGGCGCGCCCTTCCC
CGCTATCTTGCCCGAGTAGG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSRTLKIAVVDDEPDMRESISQWLALSGFDAETFATAEEALKAIGADWPGVVISDIRMPGMDGMAFLKRLMGIDSGLPVI
MITGHGDVPMAVEAMRIGAMDFMEKPFNPERLTELVKKATQARRMTLDNRALRRDLSEGQQVMSKLIGSSPVMDRLREDI
LDLGQADGHVLIDGETGTGKTLVAHALHAVGPRASKKFVPISCAAYNDEVLSAKLFGPVENGLPAVEEARGGTLCLEDIE
ALSDALQARLLAFIAEQGSPAETRIIAISNARGEGRRAEDSLRPDLFYRLTALKITLPPLRSRGEDILTLFTRMTEQFAE
EYGCEPPQVTAQEAAQLLQAPWPGNVRQLINVAERAVLQNRRGSGSITSLLMADGEDTQEATTTEGKPLKEYVESFEKML
IDNTMRRHKGSIAAVMDELCLPRRTLNEKMAKYGLSRSDYL

Sequences:

>Translated_441_residues
MSRTLKIAVVDDEPDMRESISQWLALSGFDAETFATAEEALKAIGADWPGVVISDIRMPGMDGMAFLKRLMGIDSGLPVI
MITGHGDVPMAVEAMRIGAMDFMEKPFNPERLTELVKKATQARRMTLDNRALRRDLSEGQQVMSKLIGSSPVMDRLREDI
LDLGQADGHVLIDGETGTGKTLVAHALHAVGPRASKKFVPISCAAYNDEVLSAKLFGPVENGLPAVEEARGGTLCLEDIE
ALSDALQARLLAFIAEQGSPAETRIIAISNARGEGRRAEDSLRPDLFYRLTALKITLPPLRSRGEDILTLFTRMTEQFAE
EYGCEPPQVTAQEAAQLLQAPWPGNVRQLINVAERAVLQNRRGSGSITSLLMADGEDTQEATTTEGKPLKEYVESFEKML
IDNTMRRHKGSIAAVMDELCLPRRTLNEKMAKYGLSRSDYL
>Mature_440_residues
SRTLKIAVVDDEPDMRESISQWLALSGFDAETFATAEEALKAIGADWPGVVISDIRMPGMDGMAFLKRLMGIDSGLPVIM
ITGHGDVPMAVEAMRIGAMDFMEKPFNPERLTELVKKATQARRMTLDNRALRRDLSEGQQVMSKLIGSSPVMDRLREDIL
DLGQADGHVLIDGETGTGKTLVAHALHAVGPRASKKFVPISCAAYNDEVLSAKLFGPVENGLPAVEEARGGTLCLEDIEA
LSDALQARLLAFIAEQGSPAETRIIAISNARGEGRRAEDSLRPDLFYRLTALKITLPPLRSRGEDILTLFTRMTEQFAEE
YGCEPPQVTAQEAAQLLQAPWPGNVRQLINVAERAVLQNRRGSGSITSLLMADGEDTQEATTTEGKPLKEYVESFEKMLI
DNTMRRHKGSIAAVMDELCLPRRTLNEKMAKYGLSRSDYL

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=465, Percent_Identity=32.9032258064516, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790299, Length=468, Percent_Identity=32.0512820512821, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788905, Length=452, Percent_Identity=29.4247787610619, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1790437, Length=375, Percent_Identity=32.5333333333333, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1787583, Length=326, Percent_Identity=29.4478527607362, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87082117, Length=367, Percent_Identity=32.425068119891, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789233, Length=324, Percent_Identity=32.4074074074074, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1789087, Length=337, Percent_Identity=31.4540059347181, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082152, Length=298, Percent_Identity=31.8791946308725, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1786524, Length=255, Percent_Identity=33.7254901960784, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI87081872, Length=238, Percent_Identity=32.3529411764706, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081858, Length=288, Percent_Identity=29.1666666666667, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789809, Length=117, Percent_Identity=30.7692307692308, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=114, Percent_Identity=30.7017543859649, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789828, Length=350, Percent_Identity=26, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788191, Length=119, Percent_Identity=36.9747899159664, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48387; Mature: 48256

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTLKIAVVDDEPDMRESISQWLALSGFDAETFATAEEALKAIGADWPGVVISDIRMPG
CCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
MDGMAFLKRLMGIDSGLPVIMITGHGDVPMAVEAMRIGAMDFMEKPFNPERLTELVKKAT
CCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QARRMTLDNRALRRDLSEGQQVMSKLIGSSPVMDRLREDILDLGQADGHVLIDGETGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
TLVAHALHAVGPRASKKFVPISCAAYNDEVLSAKLFGPVENGLPAVEEARGGTLCLEDIE
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCHHCCCCCHHHCCCCEEHHHHHH
ALSDALQARLLAFIAEQGSPAETRIIAISNARGEGRRAEDSLRPDLFYRLTALKITLPPL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHEEECCCC
RSRGEDILTLFTRMTEQFAEEYGCEPPQVTAQEAAQLLQAPWPGNVRQLINVAERAVLQN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
RRGSGSITSLLMADGEDTQEATTTEGKPLKEYVESFEKMLIDNTMRRHKGSIAAVMDELC
CCCCCCHHHHEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LPRRTLNEKMAKYGLSRSDYL
CCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SRTLKIAVVDDEPDMRESISQWLALSGFDAETFATAEEALKAIGADWPGVVISDIRMPG
CCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCC
MDGMAFLKRLMGIDSGLPVIMITGHGDVPMAVEAMRIGAMDFMEKPFNPERLTELVKKAT
CCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
QARRMTLDNRALRRDLSEGQQVMSKLIGSSPVMDRLREDILDLGQADGHVLIDGETGTGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
TLVAHALHAVGPRASKKFVPISCAAYNDEVLSAKLFGPVENGLPAVEEARGGTLCLEDIE
HHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHCCCHHCCCCCHHHCCCCEEHHHHHH
ALSDALQARLLAFIAEQGSPAETRIIAISNARGEGRRAEDSLRPDLFYRLTALKITLPPL
HHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHEEECCCC
RSRGEDILTLFTRMTEQFAEEYGCEPPQVTAQEAAQLLQAPWPGNVRQLINVAERAVLQN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
RRGSGSITSLLMADGEDTQEATTTEGKPLKEYVESFEKMLIDNTMRRHKGSIAAVMDELC
CCCCCCHHHHEEECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LPRRTLNEKMAKYGLSRSDYL
CCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]