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The map label for this gene is mak [H]

Identifier: 119358502

GI number: 119358502

Start: 3129175

End: 3130089

Strand: Reverse

Name: mak [H]

Synonym: Cpha266_2738

Alternate gene names: 119358502

Gene position: 3130089-3129175 (Counterclockwise)

Preceding gene: 119358503

Following gene: 119358501

Centisome position: 99.88

GC content: 53.77

Gene sequence:

>915_bases
ATGAGCCAACGATACTGGGGCATCGATCTTGGCGGAACAAAAATCGAGGTTGTTGTTACCGACAGGGCTTCCGTCCCGCT
GATTCGTCGACGGATAGCTACCGAAGCTCACAGAGGCTACCGTCACCTGCTTCTGAGGATCAGGCTGTTACTCGAAGTTG
TTTCAACAGAGAGCGGCTATCCCCTCCCCTTATGCATCGGAATGGGGACGCCGGGACGGTACGACCTGGCAACAGGAGAG
ATCAGGAACTCAAATACGACATGCCTTAACGGCCGCGATCTCAAGCGGGATCTTGAAGAACTTCTTCAAAGAGAGATGGT
GATCGAGAACGACGCGAACTGTTTTACACTTGCCGAATCAAAACTCGGATCGGGTATCGACTGCATGCGGATGCCGGGAA
AAACTGCATTTGGCATCATTCTCGGCACCGGTGTCGGCGGGGGAATCGTTTTCAGCGGTCAACTGATCCGCGGTGCACAT
GGAATTGCCGGCGAATGGGGACACAATGAACTCATTGAGGATGGAGATGCCTGCTACTGCGGGCGGAAAGGGTGCGTCGA
AACCGTTATCTCCGGCCCCGCGCTCGAACGGTATTATGGCACTCTTACCGGGAGCGTTAAACCGCTCGAAGAGATCGCCG
GATCGGTCGAAAGCGATCCGGCAGCAAGAGCGACGATCAGCCGTCTTCAGAACTATTTCGGAAAAGCGCTGGGTGCGGTA
ATCAATATACTTGATCCTGATATTTGCATCATCGGCGGTGGCGTCGGGCGCATAACGCAGCTTTATACAAGTCAAACCGT
TCGTGCGATTGAACGTCATCTATTCAACACGGAACTCCGAATTCCTTTACTACAACCCGGTCTTGGCGACAGCGCAGGTG
TTTTTGGTGCCGCGCTGTTGACTCACGGCAGATAA

Upstream 100 bases:

>100_bases
CGTTCATCGGCATATGCAGCGCGTTGTTGACCTATATTATCTGGTCATGGCTTTATAAAAATGTTATCAGGATGATGATC
ATGCGTCGGGAGTTGAAACC

Downstream 100 bases:

>100_bases
TTGCATGGCCAATGGAAAAAGAGCAGTACAGAAAAGGAGCGCTTATCGAGCTCAGCATTACCGATCTTGCCGAAAAGAAC
CAGTGCTTCGGGCGACTTGA

Product: N-acetylglucosamine kinase

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSQRYWGIDLGGTKIEVVVTDRASVPLIRRRIATEAHRGYRHLLLRIRLLLEVVSTESGYPLPLCIGMGTPGRYDLATGE
IRNSNTTCLNGRDLKRDLEELLQREMVIENDANCFTLAESKLGSGIDCMRMPGKTAFGIILGTGVGGGIVFSGQLIRGAH
GIAGEWGHNELIEDGDACYCGRKGCVETVISGPALERYYGTLTGSVKPLEEIAGSVESDPAARATISRLQNYFGKALGAV
INILDPDICIIGGGVGRITQLYTSQTVRAIERHLFNTELRIPLLQPGLGDSAGVFGAALLTHGR

Sequences:

>Translated_304_residues
MSQRYWGIDLGGTKIEVVVTDRASVPLIRRRIATEAHRGYRHLLLRIRLLLEVVSTESGYPLPLCIGMGTPGRYDLATGE
IRNSNTTCLNGRDLKRDLEELLQREMVIENDANCFTLAESKLGSGIDCMRMPGKTAFGIILGTGVGGGIVFSGQLIRGAH
GIAGEWGHNELIEDGDACYCGRKGCVETVISGPALERYYGTLTGSVKPLEEIAGSVESDPAARATISRLQNYFGKALGAV
INILDPDICIIGGGVGRITQLYTSQTVRAIERHLFNTELRIPLLQPGLGDSAGVFGAALLTHGR
>Mature_303_residues
SQRYWGIDLGGTKIEVVVTDRASVPLIRRRIATEAHRGYRHLLLRIRLLLEVVSTESGYPLPLCIGMGTPGRYDLATGEI
RNSNTTCLNGRDLKRDLEELLQREMVIENDANCFTLAESKLGSGIDCMRMPGKTAFGIILGTGVGGGIVFSGQLIRGAHG
IAGEWGHNELIEDGDACYCGRKGCVETVISGPALERYYGTLTGSVKPLEEIAGSVESDPAARATISRLQNYFGKALGAVI
NILDPDICIIGGGVGRITQLYTSQTVRAIERHLFNTELRIPLLQPGLGDSAGVFGAALLTHGR

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=270, Percent_Identity=31.1111111111111, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI298566325, Length=270, Percent_Identity=31.1111111111111, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI190014632, Length=270, Percent_Identity=31.1111111111111, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4885285, Length=270, Percent_Identity=31.1111111111111, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081733, Length=305, Percent_Identity=39.672131147541, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1787363, Length=311, Percent_Identity=32.1543408360129, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790522, Length=216, Percent_Identity=31.4814814814815, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786891, Length=202, Percent_Identity=25.7425742574257, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 32724; Mature: 32593

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRYWGIDLGGTKIEVVVTDRASVPLIRRRIATEAHRGYRHLLLRIRLLLEVVSTESGY
CCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPLCIGMGTPGRYDLATGEIRNSNTTCLNGRDLKRDLEELLQREMVIENDANCFTLAES
CCEEEEECCCCCCEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
KLGSGIDCMRMPGKTAFGIILGTGVGGGIVFSGQLIRGAHGIAGEWGHNELIEDGDACYC
HCCCCCCEEECCCCCEEEEEEECCCCCCEEEECEEEECCCCCCCCCCCCHHHCCCCEEEC
GRKGCVETVISGPALERYYGTLTGSVKPLEEIAGSVESDPAARATISRLQNYFGKALGAV
CCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
INILDPDICIIGGGVGRITQLYTSQTVRAIERHLFNTELRIPLLQPGLGDSAGVFGAALL
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEECEEECCCCCCCCHHHHHHHH
THGR
HCCC
>Mature Secondary Structure 
SQRYWGIDLGGTKIEVVVTDRASVPLIRRRIATEAHRGYRHLLLRIRLLLEVVSTESGY
CCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLPLCIGMGTPGRYDLATGEIRNSNTTCLNGRDLKRDLEELLQREMVIENDANCFTLAES
CCEEEEECCCCCCEECCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
KLGSGIDCMRMPGKTAFGIILGTGVGGGIVFSGQLIRGAHGIAGEWGHNELIEDGDACYC
HCCCCCCEEECCCCCEEEEEEECCCCCCEEEECEEEECCCCCCCCCCCCHHHCCCCEEEC
GRKGCVETVISGPALERYYGTLTGSVKPLEEIAGSVESDPAARATISRLQNYFGKALGAV
CCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
INILDPDICIIGGGVGRITQLYTSQTVRAIERHLFNTELRIPLLQPGLGDSAGVFGAALL
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEECEEECCCCCCCCHHHHHHHH
THGR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]