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The map label for this gene is murE

Identifier: 119358490

GI number: 119358490

Start: 3117226

End: 3118764

Strand: Reverse

Name: murE

Synonym: Cpha266_2726

Alternate gene names: 119358490

Gene position: 3118764-3117226 (Counterclockwise)

Preceding gene: 119358491

Following gene: 119358489

Centisome position: 99.52

GC content: 54.39

Gene sequence:

>1539_bases
ATGTCGCTCGCGGATCTCATCCGGCAGATCCCCGTACTCGAAGCGGCAGGCGGCAGCACGACGTCCCTACCGGTGAGCAT
GATCAGCTCCGACTCAAGGGATGTTCTGCCCGGAGCGCTTTTTATTGCCGTACGAGGATATTCTGCGGATGGTCATCGAT
TTATCAAGAGCGCCGTTGAGCGAGGCGCTGCGGCGGTTTTATGCGAAGAGATTCCACCTGTGCTTCCCGACGCATCGCTC
CCTTATCTCAGAGTCGAGGATGCTCGTCAGGCCCTGGCGCTTGCCGCCCGCCAGTTTTACGGTTTTGCGTCAGACGCCCT
TCACGTTATCGGCGTGACGGGAACAAACGGCAAGACAACCACCGCAAGACTTATAACGGCAATGCTCAATGCCTGCGGCA
TTCGGACGGGCTACATCGGCACAAACCTCTGTACGTTTGGCGAACAGCAGATTCCTCTTGACCGGACTACCCCTGAAGCG
CATATTCTTCACGCCCTCTTCAGGAGGATGCTTGATAGCGGATGTTCCGTAGCCGTTATGGAAGTCTCCTCGCACGCCCT
GGTGCTGCAGAGGGTATATGGCATAACCTTTCATGCGGCGGTTTTTACCAATCTGAGCATGGAGCATCTTGATTTTCATA
AAACCATGCAGGAGTATGCCGGGGCAAAACGGCAGTTGTTCGAGCAGCTTTCTCCGCAAGGTTTTGCCGTTATTAACAGT
GACGATCCGCGGGCAGAGGAGATGGTATCGAAAATAAACCCTGAAAAGAGGTTCTGCTGCACGCTTGACCAGACAGGCCG
GGTTGCTGGAGATGCCTCCCGGAGGTTTTCGGCAGAGCTCATCGATCAATCGATTGCCTCAGCAACCATACGCCTCTGTT
TTCCTGACGCACAGCAGACGATCTCTACCGTTCTTCCCGGACAGTATAACGTGATGAACCTGCTTCAGGCTGCGGCTGTC
GGTTGCGGCATGGGTCTGGGTTCGCCGGTGGTTGCGGCAGCGCTCGACGCGTTGCCGGGGGTTGCTGGCCGAATGGAGCG
GATCATGGATCGGCGACGCAAGGAGTTCGTTTTTGTTGATTATGCCCATACTCCGGATGCACTTTTCAAAGCGCTTTCAA
CCTTGAACGCACTGAAATCGCCTGCCTCCCGCCTTATCGTGGTATTCGGCTGCGGAGGAAACAGAGACCGCCTGAAGAGG
CCGGAAATGGGTAGGATAGCTGTGGAAAACGCTGACCTGGTTATTCTGACCTCCGATAATCCGCGGGATGAAGATCCGGA
GATGATTATTGATGAAATCGAGAAAGGCATAAGAGTGAAAAATCATAAAAGAATAACCGATCGAGCTCAAGCCATCAGAA
CGGCAGTCGCCCTGCTTCAGCCGGGAGACGTCCTGCTTGTGGCAGGAAAAGGTCACGAACAATATCAGGAAACTGCCGGC
GAAAAAAGCTTTTTTTCAGATCAGGAAACGCTTCGAAACGCTCTTGCTGAAGAAAATGCCGGTGAGCCGGAAAAGGGGGC
CGTATGCAAGGGGTGCTGA

Upstream 100 bases:

>100_bases
CTGTCGACAAGGAAAGCATAATCAGAGTAAAGCTTGCCATGAAAACGGTGAAGAGGACATCCCTGTGAAAAGCCTGCGCA
CTGATGATGGAAATCGGGCG

Downstream 100 bases:

>100_bases
ATATTGAAGAGCTATCGCTCAAATTCCCTGTAACCGTTGCCGGAAACGGCGATGTGCGCGGGATAGTAACGGATCCTGTT
GTGGTTATTGACTCCAGAGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSLADLIRQIPVLEAAGGSTTSLPVSMISSDSRDVLPGALFIAVRGYSADGHRFIKSAVERGAAAVLCEEIPPVLPDASL
PYLRVEDARQALALAARQFYGFASDALHVIGVTGTNGKTTTARLITAMLNACGIRTGYIGTNLCTFGEQQIPLDRTTPEA
HILHALFRRMLDSGCSVAVMEVSSHALVLQRVYGITFHAAVFTNLSMEHLDFHKTMQEYAGAKRQLFEQLSPQGFAVINS
DDPRAEEMVSKINPEKRFCCTLDQTGRVAGDASRRFSAELIDQSIASATIRLCFPDAQQTISTVLPGQYNVMNLLQAAAV
GCGMGLGSPVVAAALDALPGVAGRMERIMDRRRKEFVFVDYAHTPDALFKALSTLNALKSPASRLIVVFGCGGNRDRLKR
PEMGRIAVENADLVILTSDNPRDEDPEMIIDEIEKGIRVKNHKRITDRAQAIRTAVALLQPGDVLLVAGKGHEQYQETAG
EKSFFSDQETLRNALAEENAGEPEKGAVCKGC

Sequences:

>Translated_512_residues
MSLADLIRQIPVLEAAGGSTTSLPVSMISSDSRDVLPGALFIAVRGYSADGHRFIKSAVERGAAAVLCEEIPPVLPDASL
PYLRVEDARQALALAARQFYGFASDALHVIGVTGTNGKTTTARLITAMLNACGIRTGYIGTNLCTFGEQQIPLDRTTPEA
HILHALFRRMLDSGCSVAVMEVSSHALVLQRVYGITFHAAVFTNLSMEHLDFHKTMQEYAGAKRQLFEQLSPQGFAVINS
DDPRAEEMVSKINPEKRFCCTLDQTGRVAGDASRRFSAELIDQSIASATIRLCFPDAQQTISTVLPGQYNVMNLLQAAAV
GCGMGLGSPVVAAALDALPGVAGRMERIMDRRRKEFVFVDYAHTPDALFKALSTLNALKSPASRLIVVFGCGGNRDRLKR
PEMGRIAVENADLVILTSDNPRDEDPEMIIDEIEKGIRVKNHKRITDRAQAIRTAVALLQPGDVLLVAGKGHEQYQETAG
EKSFFSDQETLRNALAEENAGEPEKGAVCKGC
>Mature_511_residues
SLADLIRQIPVLEAAGGSTTSLPVSMISSDSRDVLPGALFIAVRGYSADGHRFIKSAVERGAAAVLCEEIPPVLPDASLP
YLRVEDARQALALAARQFYGFASDALHVIGVTGTNGKTTTARLITAMLNACGIRTGYIGTNLCTFGEQQIPLDRTTPEAH
ILHALFRRMLDSGCSVAVMEVSSHALVLQRVYGITFHAAVFTNLSMEHLDFHKTMQEYAGAKRQLFEQLSPQGFAVINSD
DPRAEEMVSKINPEKRFCCTLDQTGRVAGDASRRFSAELIDQSIASATIRLCFPDAQQTISTVLPGQYNVMNLLQAAAVG
CGMGLGSPVVAAALDALPGVAGRMERIMDRRRKEFVFVDYAHTPDALFKALSTLNALKSPASRLIVVFGCGGNRDRLKRP
EMGRIAVENADLVILTSDNPRDEDPEMIIDEIEKGIRVKNHKRITDRAQAIRTAVALLQPGDVLLVAGKGHEQYQETAGE
KSFFSDQETLRNALAEENAGEPEKGAVCKGC

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=473, Percent_Identity=37.6321353065539, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI1786274, Length=366, Percent_Identity=27.5956284153005, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CHLPD (A1BJY3)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_913134.1
- ProteinModelPortal:   A1BJY3
- SMR:   A1BJY3
- STRING:   A1BJY3
- GeneID:   4570700
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_2726
- NMPDR:   fig|290317.7.peg.2847
- eggNOG:   COG0769
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   CLSK2320859
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 55402; Mature: 55271

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: BINDING 32-32 BINDING 183-183 BINDING 191-191 BINDING 395-395 BINDING 469-469 BINDING 473-473

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLADLIRQIPVLEAAGGSTTSLPVSMISSDSRDVLPGALFIAVRGYSADGHRFIKSAVE
CCHHHHHHHCCCEECCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
RGAAAVLCEEIPPVLPDASLPYLRVEDARQALALAARQFYGFASDALHVIGVTGTNGKTT
CCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
TARLITAMLNACGIRTGYIGTNLCTFGEQQIPLDRTTPEAHILHALFRRMLDSGCSVAVM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
EVSSHALVLQRVYGITFHAAVFTNLSMEHLDFHKTMQEYAGAKRQLFEQLSPQGFAVINS
EHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECC
DDPRAEEMVSKINPEKRFCCTLDQTGRVAGDASRRFSAELIDQSIASATIRLCFPDAQQT
CCCHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHH
ISTVLPGQYNVMNLLQAAAVGCGMGLGSPVVAAALDALPGVAGRMERIMDRRRKEFVFVD
HHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEE
YAHTPDALFKALSTLNALKSPASRLIVVFGCGGNRDRLKRPEMGRIAVENADLVILTSDN
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCEEEECCCEEEEECCC
PRDEDPEMIIDEIEKGIRVKNHKRITDRAQAIRTAVALLQPGDVLLVAGKGHEQYQETAG
CCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCC
EKSFFSDQETLRNALAEENAGEPEKGAVCKGC
CHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLADLIRQIPVLEAAGGSTTSLPVSMISSDSRDVLPGALFIAVRGYSADGHRFIKSAVE
CHHHHHHHCCCEECCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHH
RGAAAVLCEEIPPVLPDASLPYLRVEDARQALALAARQFYGFASDALHVIGVTGTNGKTT
CCHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHH
TARLITAMLNACGIRTGYIGTNLCTFGEQQIPLDRTTPEAHILHALFRRMLDSGCSVAVM
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEE
EVSSHALVLQRVYGITFHAAVFTNLSMEHLDFHKTMQEYAGAKRQLFEQLSPQGFAVINS
EHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECC
DDPRAEEMVSKINPEKRFCCTLDQTGRVAGDASRRFSAELIDQSIASATIRLCFPDAQQT
CCCHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCHHHH
ISTVLPGQYNVMNLLQAAAVGCGMGLGSPVVAAALDALPGVAGRMERIMDRRRKEFVFVD
HHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCEEEEE
YAHTPDALFKALSTLNALKSPASRLIVVFGCGGNRDRLKRPEMGRIAVENADLVILTSDN
ECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHCCCCCCCCEEEECCCEEEEECCC
PRDEDPEMIIDEIEKGIRVKNHKRITDRAQAIRTAVALLQPGDVLLVAGKGHEQYQETAG
CCCCCHHHHHHHHHHCCEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHCC
EKSFFSDQETLRNALAEENAGEPEKGAVCKGC
CHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA