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The map label for this gene is murF [H]

Identifier: 119358489

GI number: 119358489

Start: 3115823

End: 3117241

Strand: Reverse

Name: murF [H]

Synonym: Cpha266_2725

Alternate gene names: 119358489

Gene position: 3117241-3115823 (Counterclockwise)

Preceding gene: 119358490

Following gene: 119358488

Centisome position: 99.47

GC content: 54.69

Gene sequence:

>1419_bases
ATGCAAGGGGTGCTGAATATTGAAGAGCTATCGCTCAAATTCCCTGTAACCGTTGCCGGAAACGGCGATGTGCGCGGGAT
AGTAACGGATCCTGTTGTGGTTATTGACTCCAGAGCGGTCGTCGACGGCGCGATATTTGTTGCTCTGCAGGGAGAGCATA
CCGACGGGCATCAGTACATCGACGAGGTGTTTGCCAAAGGCGCTCATTGGGCGATGGTGAAAAAGGCATGGTATGAATCA
GAAGGGGCTCCCGCCGCGCCGGAAGGGTGCGGCTTTCTGGTCGTCGAGGATCCTGTCGCCGGACTTCAGCGGCTTGCGGC
CATCTACCGGCAAACCTTTTCGATTCCGGTTATTGCCGTCGGAGGCAGTAATGGAAAAACAACAACGAAAGAGATGGTTG
CGGCCGTTCTTGGTACGGGATTTACGGTTCAGACGAGCAAAGGGAACCTGAACAACCATCTTGGCGTCCCCTTGACCCTG
CTGCAGCTCCGTCGGCAAACCGGGATTGCCGTCGTCGAAATGGGTATCAATCATCCCGGCGAGATGGAACTGCTCCTTGA
ACTGGCCCGCCCGACCCATGGCCTTCTGACCAATATCGGCCACGAACATCTGGAGTTTCTTCTGAACCTCGAAGGCGTTG
CCGCCGAAGAAACCAGGCTTTTCAGATACCTTCAACAGCATGGAGGCACCTGCTTTGTCAATGCAGACGATCTCTGGTTG
AAGGAAGCCGCCGAAGGATTGACCGGCAGTTTTACATACGGAACTGCTGACGACAGCCAGATAGCCTGCCAAGCGCGCCA
TATTGCTGTCGACCGATCAGGACGAGTCTCCTTCGAGTTTGTTTCCGGCGGGTTGTCCGAAAGAATCGCCCTGCATTTTA
TAGGAAAACACAACGTCATCAACGCACTGGCCGCCGCGGCAGTGGGCACTTATTTCGGCCTTTCGCTTGAGCAGATCAAA
AAAGGGCTCGAAGCGCTGCGTCCATCCCCCGGCTGGAAACGCCTTGAAGTCCAGGACGCGGGAGGCGTCGTGCTGATCAA
CGATACCTACAACGCCAATTCAGACTCCATGCGTCTTGCCATCGATGCGCTTGTCGATATACCGTGCAGTGGCAAAAAAA
TCGCCGTGCTCGGCGATATGCTTGAACTTGGCGAAGCGGGAATAGTTGAACATGAAAAAACAGGCAGGTATATTCAGCAA
AGTCCGATCGACCTGCTGTTTACCTATGGGCGACATGCCCGGCTCTACGGTATTGAGGCCGGAGCCCGCTGCCACGGCCA
TTACGAAAATCGGCAAAAGCTGCTGGATGAGTTGAAGATGGTCGTTCAGCCGGGTGACGCCGTGCTGTTCAAGGCATCAA
GAAGTATGCGGCTTGAGCTGGTTGTCGAGGCGCTGATCAAAGAAAGAACAACAATGTAG

Upstream 100 bases:

>100_bases
ATCAGGAAACTGCCGGCGAAAAAAGCTTTTTTTCAGATCAGGAAACGCTTCGAAACGCTCTTGCTGAAGAAAATGCCGGT
GAGCCGGAAAAGGGGGCCGT

Downstream 100 bases:

>100_bases
AAAGGGCACGGCATGCTTTATTATCTTCTGAAATACATTAACGATGTGTTTGATCCCCCGGGGTTCGGCGTCATCGAATT
CCTGACGTTCAGGGCAAGCG

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MQGVLNIEELSLKFPVTVAGNGDVRGIVTDPVVVIDSRAVVDGAIFVALQGEHTDGHQYIDEVFAKGAHWAMVKKAWYES
EGAPAAPEGCGFLVVEDPVAGLQRLAAIYRQTFSIPVIAVGGSNGKTTTKEMVAAVLGTGFTVQTSKGNLNNHLGVPLTL
LQLRRQTGIAVVEMGINHPGEMELLLELARPTHGLLTNIGHEHLEFLLNLEGVAAEETRLFRYLQQHGGTCFVNADDLWL
KEAAEGLTGSFTYGTADDSQIACQARHIAVDRSGRVSFEFVSGGLSERIALHFIGKHNVINALAAAAVGTYFGLSLEQIK
KGLEALRPSPGWKRLEVQDAGGVVLINDTYNANSDSMRLAIDALVDIPCSGKKIAVLGDMLELGEAGIVEHEKTGRYIQQ
SPIDLLFTYGRHARLYGIEAGARCHGHYENRQKLLDELKMVVQPGDAVLFKASRSMRLELVVEALIKERTTM

Sequences:

>Translated_472_residues
MQGVLNIEELSLKFPVTVAGNGDVRGIVTDPVVVIDSRAVVDGAIFVALQGEHTDGHQYIDEVFAKGAHWAMVKKAWYES
EGAPAAPEGCGFLVVEDPVAGLQRLAAIYRQTFSIPVIAVGGSNGKTTTKEMVAAVLGTGFTVQTSKGNLNNHLGVPLTL
LQLRRQTGIAVVEMGINHPGEMELLLELARPTHGLLTNIGHEHLEFLLNLEGVAAEETRLFRYLQQHGGTCFVNADDLWL
KEAAEGLTGSFTYGTADDSQIACQARHIAVDRSGRVSFEFVSGGLSERIALHFIGKHNVINALAAAAVGTYFGLSLEQIK
KGLEALRPSPGWKRLEVQDAGGVVLINDTYNANSDSMRLAIDALVDIPCSGKKIAVLGDMLELGEAGIVEHEKTGRYIQQ
SPIDLLFTYGRHARLYGIEAGARCHGHYENRQKLLDELKMVVQPGDAVLFKASRSMRLELVVEALIKERTTM
>Mature_472_residues
MQGVLNIEELSLKFPVTVAGNGDVRGIVTDPVVVIDSRAVVDGAIFVALQGEHTDGHQYIDEVFAKGAHWAMVKKAWYES
EGAPAAPEGCGFLVVEDPVAGLQRLAAIYRQTFSIPVIAVGGSNGKTTTKEMVAAVLGTGFTVQTSKGNLNNHLGVPLTL
LQLRRQTGIAVVEMGINHPGEMELLLELARPTHGLLTNIGHEHLEFLLNLEGVAAEETRLFRYLQQHGGTCFVNADDLWL
KEAAEGLTGSFTYGTADDSQIACQARHIAVDRSGRVSFEFVSGGLSERIALHFIGKHNVINALAAAAVGTYFGLSLEQIK
KGLEALRPSPGWKRLEVQDAGGVVLINDTYNANSDSMRLAIDALVDIPCSGKKIAVLGDMLELGEAGIVEHEKTGRYIQQ
SPIDLLFTYGRHARLYGIEAGARCHGHYENRQKLLDELKMVVQPGDAVLFKASRSMRLELVVEALIKERTTM

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=454, Percent_Identity=34.1409691629956, Blast_Score=189, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51112; Mature: 51112

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGVLNIEELSLKFPVTVAGNGDVRGIVTDPVVVIDSRAVVDGAIFVALQGEHTDGHQYI
CCCCCCHHHEEEEEEEEEECCCCEEEEEECCEEEEECCEEECEEEEEEEECCCCCHHHHH
DEVFAKGAHWAMVKKAWYESEGAPAAPEGCGFLVVEDPVAGLQRLAAIYRQTFSIPVIAV
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
GGSNGKTTTKEMVAAVLGTGFTVQTSKGNLNNHLGVPLTLLQLRRQTGIAVVEMGINHPG
ECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
EMELLLELARPTHGLLTNIGHEHLEFLLNLEGVAAEETRLFRYLQQHGGTCFVNADDLWL
HHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHH
KEAAEGLTGSFTYGTADDSQIACQARHIAVDRSGRVSFEFVSGGLSERIALHFIGKHNVI
HHHHCCCCCCEEECCCCCCHHEEEEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHH
NALAAAAVGTYFGLSLEQIKKGLEALRPSPGWKRLEVQDAGGVVLINDTYNANSDSMRLA
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEE
IDALVDIPCSGKKIAVLGDMLELGEAGIVEHEKTGRYIQQSPIDLLFTYGRHARLYGIEA
EEEHEECCCCCCEEEEEHHHHHCCCCCCEECCCCCCHHHCCCCCEEEECCCCEEEEEECC
GARCHGHYENRQKLLDELKMVVQPGDAVLFKASRSMRLELVVEALIKERTTM
CCEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQGVLNIEELSLKFPVTVAGNGDVRGIVTDPVVVIDSRAVVDGAIFVALQGEHTDGHQYI
CCCCCCHHHEEEEEEEEEECCCCEEEEEECCEEEEECCEEECEEEEEEEECCCCCHHHHH
DEVFAKGAHWAMVKKAWYESEGAPAAPEGCGFLVVEDPVAGLQRLAAIYRQTFSIPVIAV
HHHHHCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCCCEEEEE
GGSNGKTTTKEMVAAVLGTGFTVQTSKGNLNNHLGVPLTLLQLRRQTGIAVVEMGINHPG
ECCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
EMELLLELARPTHGLLTNIGHEHLEFLLNLEGVAAEETRLFRYLQQHGGTCFVNADDLWL
HHHHHHHHHCCCHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHH
KEAAEGLTGSFTYGTADDSQIACQARHIAVDRSGRVSFEFVSGGLSERIALHFIGKHNVI
HHHHCCCCCCEEECCCCCCHHEEEEEEEEEECCCCEEEEEECCCCCCEEEEEEECCCHHH
NALAAAAVGTYFGLSLEQIKKGLEALRPSPGWKRLEVQDAGGVVLINDTYNANSDSMRLA
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCEEEE
IDALVDIPCSGKKIAVLGDMLELGEAGIVEHEKTGRYIQQSPIDLLFTYGRHARLYGIEA
EEEHEECCCCCCEEEEEHHHHHCCCCCCEECCCCCCHHHCCCCCEEEECCCCEEEEEECC
GARCHGHYENRQKLLDELKMVVQPGDAVLFKASRSMRLELVVEALIKERTTM
CCEECCCHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]