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The map label for this gene is prpE [H]

Identifier: 119358361

GI number: 119358361

Start: 2971423

End: 2972115

Strand: Reverse

Name: prpE [H]

Synonym: Cpha266_2593

Alternate gene names: 119358361

Gene position: 2972115-2971423 (Counterclockwise)

Preceding gene: 119358362

Following gene: 119358360

Centisome position: 94.84

GC content: 45.17

Gene sequence:

>693_bases
ATGGGTTCTGTTTTGTCGGAAAATCGTCGCATTATTGCCATTGGTGATGTGCATGGCTGTTTGTGTTCGCTCAAGGAGCT
TCTTGCCCGAATCGTTCTCGAACCTGATGATCAACTGGTGTTTTCAGGCGATATGATTGATCGCGGGGCCTGTTCAAAAG
ATGTTGTTGAGTATCTTTTGGAGCTTGGTTCGCGGTTCTCCTGTTTTTTTCTTATGGGCAATCATGAGCTGATGCTTCTT
GATTATCTTGTTACTCGCGTACCTGATATGTGGTTTTTTGGCGGGGGAGAGGCTACTCTTCGTTCTTATAATAGTCCGGA
CGGATTTGATCTGCCTTCTGAGCATCTGGATTTTTTCAGGGGGTGTAGTTATTTTATTGAGACGGAGAACTTTTTTTTTA
CGCATGGAGGGCTTGATCCTGAGCTTTCCATCAGGGATAATCTTCGTTATTGCAAGCCGGAGGAGTTTTGTTGGCAGCGG
GCTCATATGCGGAGCTCTTTTCTTGAGAGAAATTCTTACAATTGGCAGAAAACGGTTGTTTGTGCTCATACGCCGGTTTC
TGAGCCGGTGTTGCTTGATCGGCTTATCGCTATTGATACCGGGTGCGTTTATAAGGATAATCCTTTGCTTGGTAAGTTGA
CGGCTGTTATTCTTCCGGAACGGCGGATTGTTCAGGTTTCGAATTGTGATTGA

Upstream 100 bases:

>100_bases
TTGTGATGGTTTTATGCGCTGCAATGACTATGCATGGAGGATTGATTTTTTTATTTTTTGCTTTTTCGGGGGAGCGTAAC
GTTATGTTTTTTGATACGTA

Downstream 100 bases:

>100_bases
GCGGGCGATTTGCTCGCTAATTGGGATGAAGAGACGAATGGGATGAAGAGGGGGAGTTTGCCCACGAATTGACACGAATT
TGCGCGAAAGGAAATTAAGG

Product: metallophosphoesterase

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MGSVLSENRRIIAIGDVHGCLCSLKELLARIVLEPDDQLVFSGDMIDRGACSKDVVEYLLELGSRFSCFFLMGNHELMLL
DYLVTRVPDMWFFGGGEATLRSYNSPDGFDLPSEHLDFFRGCSYFIETENFFFTHGGLDPELSIRDNLRYCKPEEFCWQR
AHMRSSFLERNSYNWQKTVVCAHTPVSEPVLLDRLIAIDTGCVYKDNPLLGKLTAVILPERRIVQVSNCD

Sequences:

>Translated_230_residues
MGSVLSENRRIIAIGDVHGCLCSLKELLARIVLEPDDQLVFSGDMIDRGACSKDVVEYLLELGSRFSCFFLMGNHELMLL
DYLVTRVPDMWFFGGGEATLRSYNSPDGFDLPSEHLDFFRGCSYFIETENFFFTHGGLDPELSIRDNLRYCKPEEFCWQR
AHMRSSFLERNSYNWQKTVVCAHTPVSEPVLLDRLIAIDTGCVYKDNPLLGKLTAVILPERRIVQVSNCD
>Mature_229_residues
GSVLSENRRIIAIGDVHGCLCSLKELLARIVLEPDDQLVFSGDMIDRGACSKDVVEYLLELGSRFSCFFLMGNHELMLLD
YLVTRVPDMWFFGGGEATLRSYNSPDGFDLPSEHLDFFRGCSYFIETENFFFTHGGLDPELSIRDNLRYCKPEEFCWQRA
HMRSSFLERNSYNWQKTVVCAHTPVSEPVLLDRLIAIDTGCVYKDNPLLGKLTAVILPERRIVQVSNCD

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 26252; Mature: 26121

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
4.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVLSENRRIIAIGDVHGCLCSLKELLARIVLEPDDQLVFSGDMIDRGACSKDVVEYLL
CCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
ELGSRFSCFFLMGNHELMLLDYLVTRVPDMWFFGGGEATLRSYNSPDGFDLPSEHLDFFR
HHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHEEECCCCCCCCCCCHHHHHHHH
GCSYFIETENFFFTHGGLDPELSIRDNLRYCKPEEFCWQRAHMRSSFLERNSYNWQKTVV
CCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
CAHTPVSEPVLLDRLIAIDTGCVYKDNPLLGKLTAVILPERRIVQVSNCD
EEECCCCCHHHHHHHHHHCCCCEEECCCCHHEEEEEEECCCEEEEECCCC
>Mature Secondary Structure 
GSVLSENRRIIAIGDVHGCLCSLKELLARIVLEPDDQLVFSGDMIDRGACSKDVVEYLL
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHHHHHHHH
ELGSRFSCFFLMGNHELMLLDYLVTRVPDMWFFGGGEATLRSYNSPDGFDLPSEHLDFFR
HHCCCEEEEEEECCCCCHHHHHHHHHCCCEEEECCCHHEEECCCCCCCCCCCHHHHHHHH
GCSYFIETENFFFTHGGLDPELSIRDNLRYCKPEEFCWQRAHMRSSFLERNSYNWQKTVV
CCCEEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEE
CAHTPVSEPVLLDRLIAIDTGCVYKDNPLLGKLTAVILPERRIVQVSNCD
EEECCCCCHHHHHHHHHHCCCCEEECCCCHHEEEEEEECCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]