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The map label for this gene is nfo [H]

Identifier: 119357774

GI number: 119357774

Start: 2295039

End: 2295893

Strand: Direct

Name: nfo [H]

Synonym: Cpha266_1981

Alternate gene names: 119357774

Gene position: 2295039-2295893 (Clockwise)

Preceding gene: 119357772

Following gene: 119357778

Centisome position: 73.23

GC content: 51.81

Gene sequence:

>855_bases
ATGAAACGTGTCGGAGCACATGTCAGTATTGCGGGAGGCATCGAAAATGCTCCGCTGAACGCCAGCAGCATTGGCGCAAC
AGCATTCGCATGCTTTACAAAAAATCAGCGGCAGTGGAAATCTCCTGAAATCTCCCCGGCGTCAATAGAGCTATTCGAAA
AAAACTGCGCTACTGCCGGGATCAGGTCTGAACACATCCTCCCGCACGACAGCTACCTCATCAACCTCGGCAACCCCGAT
CCCGACAAACTGGAGCGATCAAGAAATGCGTTTATTGATGAAATTCGTCGGGCGGCAAGTCTCGGCCTCGTCATGCTGAA
CTTCCATCCGGGAAGCCACCTCAATCAGATTGATGAAAAGGGCTGTTTGCAGCTTATTGCCGATTCTGTTAACATCGCTC
TCGAAAAAACCTCCGGCGTCACAGCCGTCATCGAAAACACCGCCGGACAGGGAAGCAACCTCGGCAACAGCTTTGAACAA
CTTGCCTCGATCATTGATAAAATCGACGATAAATCCAGAGTCGGGGTTTGTCTTGACACCTGCCATCTTTTTGCGAGCGG
CTACGACCTGCAATCAAGCGAAGCCGTAACGGCAATGTTCGAAGCATTTGACCGGACTGTCGGGCTCTCCAACCTGAGAG
GAATGCACCTCAACGATGCGAAACACCCGCTCGGAAGCCATCTTGACCGACATGCCTGTATCGGAAAAGGAGCGATCGGG
CTCAGCGCCTTCGCATCTATCATGAACAGCCCATTCTGCGAGGAGATCCCCCTCATCCTTGAAACACCGGATGCGGAGGG
TTGGAAAGAGGAGATAGTTCTTCTCTATTCACTCATAGACCATCCGATCAAATAG

Upstream 100 bases:

>100_bases
TCCTTCCGGTTATTTGTTGGCAGTTGTCGATGCACTCGTTACCTTACCCCTCGGATCAGTTATCTCGCCGAAAATATCCG
CAAAACGTAAAACTCTTGTT

Downstream 100 bases:

>100_bases
CGATACCCGGGAGTGTATTGGCCCTATCGCTTTGAAACCAGCCTGAGATACTTGCTCACCGACAGCATGCTGCCAATAAC
GCCAAGCGACAGGCCAAGAA

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKRVGAHVSIAGGIENAPLNASSIGATAFACFTKNQRQWKSPEISPASIELFEKNCATAGIRSEHILPHDSYLINLGNPD
PDKLERSRNAFIDEIRRAASLGLVMLNFHPGSHLNQIDEKGCLQLIADSVNIALEKTSGVTAVIENTAGQGSNLGNSFEQ
LASIIDKIDDKSRVGVCLDTCHLFASGYDLQSSEAVTAMFEAFDRTVGLSNLRGMHLNDAKHPLGSHLDRHACIGKGAIG
LSAFASIMNSPFCEEIPLILETPDAEGWKEEIVLLYSLIDHPIK

Sequences:

>Translated_284_residues
MKRVGAHVSIAGGIENAPLNASSIGATAFACFTKNQRQWKSPEISPASIELFEKNCATAGIRSEHILPHDSYLINLGNPD
PDKLERSRNAFIDEIRRAASLGLVMLNFHPGSHLNQIDEKGCLQLIADSVNIALEKTSGVTAVIENTAGQGSNLGNSFEQ
LASIIDKIDDKSRVGVCLDTCHLFASGYDLQSSEAVTAMFEAFDRTVGLSNLRGMHLNDAKHPLGSHLDRHACIGKGAIG
LSAFASIMNSPFCEEIPLILETPDAEGWKEEIVLLYSLIDHPIK
>Mature_284_residues
MKRVGAHVSIAGGIENAPLNASSIGATAFACFTKNQRQWKSPEISPASIELFEKNCATAGIRSEHILPHDSYLINLGNPD
PDKLERSRNAFIDEIRRAASLGLVMLNFHPGSHLNQIDEKGCLQLIADSVNIALEKTSGVTAVIENTAGQGSNLGNSFEQ
LASIIDKIDDKSRVGVCLDTCHLFASGYDLQSSEAVTAMFEAFDRTVGLSNLRGMHLNDAKHPLGSHLDRHACIGKGAIG
LSAFASIMNSPFCEEIPLILETPDAEGWKEEIVLLYSLIDHPIK

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788483, Length=275, Percent_Identity=59.2727272727273, Blast_Score=331, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17531193, Length=277, Percent_Identity=43.6823104693141, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322735, Length=276, Percent_Identity=42.0289855072464, Blast_Score=236, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307 [H]

Pfam domain/function: PF01261 AP_endonuc_2 [H]

EC number: =3.1.21.2 [H]

Molecular weight: Translated: 30674; Mature: 30674

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00729 AP_NUCLEASE_F2_1 ; PS00730 AP_NUCLEASE_F2_2 ; PS00731 AP_NUCLEASE_F2_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVGAHVSIAGGIENAPLNASSIGATAFACFTKNQRQWKSPEISPASIELFEKNCATAG
CCCCCCEEEEECCCCCCCCCCHHHCHHHHEEECCCCHHCCCCCCCCHHHHHHHHHHHHCC
IRSEHILPHDSYLINLGNPDPDKLERSRNAFIDEIRRAASLGLVMLNFHPGSHLNQIDEK
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
GCLQLIADSVNIALEKTSGVTAVIENTAGQGSNLGNSFEQLASIIDKIDDKSRVGVCLDT
HHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
CHLFASGYDLQSSEAVTAMFEAFDRTVGLSNLRGMHLNDAKHPLGSHLDRHACIGKGAIG
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
LSAFASIMNSPFCEEIPLILETPDAEGWKEEIVLLYSLIDHPIK
HHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRVGAHVSIAGGIENAPLNASSIGATAFACFTKNQRQWKSPEISPASIELFEKNCATAG
CCCCCCEEEEECCCCCCCCCCHHHCHHHHEEECCCCHHCCCCCCCCHHHHHHHHHHHHCC
IRSEHILPHDSYLINLGNPDPDKLERSRNAFIDEIRRAASLGLVMLNFHPGSHLNQIDEK
CCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHH
GCLQLIADSVNIALEKTSGVTAVIENTAGQGSNLGNSFEQLASIIDKIDDKSRVGVCLDT
HHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
CHLFASGYDLQSSEAVTAMFEAFDRTVGLSNLRGMHLNDAKHPLGSHLDRHACIGKGAIG
HHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHH
LSAFASIMNSPFCEEIPLILETPDAEGWKEEIVLLYSLIDHPIK
HHHHHHHHCCCCHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA