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The map label for this gene is proB [H]

Identifier: 119357660

GI number: 119357660

Start: 2160053

End: 2161141

Strand: Direct

Name: proB [H]

Synonym: Cpha266_1864

Alternate gene names: 119357660

Gene position: 2160053-2161141 (Clockwise)

Preceding gene: 119357659

Following gene: 119357661

Centisome position: 68.93

GC content: 49.13

Gene sequence:

>1089_bases
ATGAATAATCAGGAACTACTGTACAAAAAAATAGTTGTCAAAGTCGGAACCAATGTCATCACGGACAAACATGGCGACCT
CGATCTTGACATTCTTAAAAAGCTCACCACCCAGATCGCACTGTTACAAAAGGAGGGGGTGCAGATTATTCTGGTTTCGT
CCGGTGCCGTCGGGGCAGGGCGCTCCATTGTAAAGCTGCCGGAAACCCTTTCGCCGGTTGCAACCCGTCAGGTTCTTGCC
GCTACCGGCCAGATAAAGCTCATCAATACCTACAATACGCTGTTTCTTGAACAGGGGCTCGTCAGTGCTCAGATTCTGGT
AACCAAGAGTGATTTCAGGGATCGACAACACTACCTGAACATGCGCACCTGTTTTACCTCTCTGCTGCAGCAACATATCA
TTCCTGTTGTCAACGAAAACGATGCGGTATCTGTAACCGAACTGATGTTTACCGACAATGACGAACTCTCGGGCCTCATT
GCTTCAATGATGCATGTTGAGGGCTATATCATACTGTCGCACGTTGACGGACTTTTTGACCTGAAAAACGGAACGGGCAA
GCTTATCACGGAAATTGACCCTGCAACGAAACACTTCAACCAACATATTATTCCCGGAAAATCAGAGTTCGGACGAGGGG
GGATGCTGACGAAATGCCACATAGCCCACAAGCTTTCGCAACTCGGCGTCACCGTTCATATTGCCAATGGGAAAACTCCC
GGCATTCTCCTCTCCCTGCTTCGCGGAGAAAAGGCCGGCACGAAATTTCTCGCCCGAAAAAAGCCTGTTTTAGGCCCTAA
ACTCTGGGTTGCACACAGCGAAGGGCTTGAAAAAGGGGCGGTAATCGTCAACGAAGGGGCCGAAAAGGTACTCTCCGACT
CCGACAGGGCAAACAGTCTGCTGCCGGTAGGCATCGATTCGGTTCAGGGAACGTTTAAAAAAGGGGACATTATAAAAATC
CTCGGCATCAACAAAAAGGTGATCGGCTATGGAATGGCGCAATACGGTTCCGAAAAAACCCGGACCCTTATGGGTCAACA
GGATCAGAAGCCGCTGATTCACTACGATTATCTCTATATCACGCACTGA

Upstream 100 bases:

>100_bases
GTTTGGCGAAGGAATCAACATCGTTAAAACAATTTCCCTGCTTTTTATTATTATCGGTGTGGTCGGGCTGCATTTCAGTC
AGGAACACCTCCAATGACTT

Downstream 100 bases:

>100_bases
AAAGCAGAACAACAGAACCTATCTGATAAACGAACCAATGCAGAGAATCATTACGGAAAAGCTCAAGGCGGTCCGGGAGG
CAAGCCGAAAGATCATTACT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MNNQELLYKKIVVKVGTNVITDKHGDLDLDILKKLTTQIALLQKEGVQIILVSSGAVGAGRSIVKLPETLSPVATRQVLA
ATGQIKLINTYNTLFLEQGLVSAQILVTKSDFRDRQHYLNMRTCFTSLLQQHIIPVVNENDAVSVTELMFTDNDELSGLI
ASMMHVEGYIILSHVDGLFDLKNGTGKLITEIDPATKHFNQHIIPGKSEFGRGGMLTKCHIAHKLSQLGVTVHIANGKTP
GILLSLLRGEKAGTKFLARKKPVLGPKLWVAHSEGLEKGAVIVNEGAEKVLSDSDRANSLLPVGIDSVQGTFKKGDIIKI
LGINKKVIGYGMAQYGSEKTRTLMGQQDQKPLIHYDYLYITH

Sequences:

>Translated_362_residues
MNNQELLYKKIVVKVGTNVITDKHGDLDLDILKKLTTQIALLQKEGVQIILVSSGAVGAGRSIVKLPETLSPVATRQVLA
ATGQIKLINTYNTLFLEQGLVSAQILVTKSDFRDRQHYLNMRTCFTSLLQQHIIPVVNENDAVSVTELMFTDNDELSGLI
ASMMHVEGYIILSHVDGLFDLKNGTGKLITEIDPATKHFNQHIIPGKSEFGRGGMLTKCHIAHKLSQLGVTVHIANGKTP
GILLSLLRGEKAGTKFLARKKPVLGPKLWVAHSEGLEKGAVIVNEGAEKVLSDSDRANSLLPVGIDSVQGTFKKGDIIKI
LGINKKVIGYGMAQYGSEKTRTLMGQQDQKPLIHYDYLYITH
>Mature_362_residues
MNNQELLYKKIVVKVGTNVITDKHGDLDLDILKKLTTQIALLQKEGVQIILVSSGAVGAGRSIVKLPETLSPVATRQVLA
ATGQIKLINTYNTLFLEQGLVSAQILVTKSDFRDRQHYLNMRTCFTSLLQQHIIPVVNENDAVSVTELMFTDNDELSGLI
ASMMHVEGYIILSHVDGLFDLKNGTGKLITEIDPATKHFNQHIIPGKSEFGRGGMLTKCHIAHKLSQLGVTVHIANGKTP
GILLSLLRGEKAGTKFLARKKPVLGPKLWVAHSEGLEKGAVIVNEGAEKVLSDSDRANSLLPVGIDSVQGTFKKGDIIKI
LGINKKVIGYGMAQYGSEKTRTLMGQQDQKPLIHYDYLYITH

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=297, Percent_Identity=34.6801346801347, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI62912457, Length=295, Percent_Identity=34.5762711864407, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786437, Length=357, Percent_Identity=34.1736694677871, Blast_Score=201, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=34.9090909090909, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=34.9090909090909, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=28.8135593220339, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=354, Percent_Identity=29.6610169491525, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=31.0104529616725, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39634; Mature: 39634

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNQELLYKKIVVKVGTNVITDKHGDLDLDILKKLTTQIALLQKEGVQIILVSSGAVGAG
CCCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RSIVKLPETLSPVATRQVLAATGQIKLINTYNTLFLEQGLVSAQILVTKSDFRDRQHYLN
CCEEECCHHHCHHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
MRTCFTSLLQQHIIPVVNENDAVSVTELMFTDNDELSGLIASMMHVEGYIILSHVDGLFD
HHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEE
LKNGTGKLITEIDPATKHFNQHIIPGKSEFGRGGMLTKCHIAHKLSQLGVTVHIANGKTP
ECCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEECCCCCH
GILLSLLRGEKAGTKFLARKKPVLGPKLWVAHSEGLEKGAVIVNEGAEKVLSDSDRANSL
HHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHCCCCCCCCE
LPVGIDSVQGTFKKGDIIKILGINKKVIGYGMAQYGSEKTRTLMGQQDQKPLIHYDYLYI
EEECCHHHCCCCCCCCEEEEEECCCHHHHCCHHHHCCHHHHHHCCCCCCCCEEEEEEEEE
TH
EC
>Mature Secondary Structure
MNNQELLYKKIVVKVGTNVITDKHGDLDLDILKKLTTQIALLQKEGVQIILVSSGAVGAG
CCCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
RSIVKLPETLSPVATRQVLAATGQIKLINTYNTLFLEQGLVSAQILVTKSDFRDRQHYLN
CCEEECCHHHCHHHHHHHHHCCCCEEEEECHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
MRTCFTSLLQQHIIPVVNENDAVSVTELMFTDNDELSGLIASMMHVEGYIILSHVDGLFD
HHHHHHHHHHHHCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEEECCCEEE
LKNGTGKLITEIDPATKHFNQHIIPGKSEFGRGGMLTKCHIAHKLSQLGVTVHIANGKTP
ECCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCEEEEECCCCCH
GILLSLLRGEKAGTKFLARKKPVLGPKLWVAHSEGLEKGAVIVNEGAEKVLSDSDRANSL
HHHHHHHHCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCEEEECCHHHHHCCCCCCCCE
LPVGIDSVQGTFKKGDIIKILGINKKVIGYGMAQYGSEKTRTLMGQQDQKPLIHYDYLYI
EEECCHHHCCCCCCCCEEEEEECCCHHHHCCHHHHCCHHHHHHCCCCCCCCEEEEEEEEE
TH
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901 [H]