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The map label for this gene is pyc [H]

Identifier: 119356885

GI number: 119356885

Start: 1202745

End: 1204775

Strand: Reverse

Name: pyc [H]

Synonym: Cpha266_1063

Alternate gene names: 119356885

Gene position: 1204775-1202745 (Counterclockwise)

Preceding gene: 119356886

Following gene: 119356882

Centisome position: 38.44

GC content: 51.6

Gene sequence:

>2031_bases
ATGACCAATTCTGTCTTCGTCAAACCGGGAATGAGCCCGAAAGAAATCATCAATAACGTTCGTCATTTAGATGCGATAGC
CCTGACTTCGACCGGGATGAGAGACGCCGGACAGTCGGATTATAAAAACCGGCTGAGAATACGCGACCTCTCCACCCTTT
CTTCGAACTACAATGAAATGGGGCTGTTCAGTTCGGAGTGCCATGGCGGAGCGCGCTGGCATGTCGGGATCATGAACCGT
CGGGAAAGCCCGTTTGAAGAAATTGCGCTGCTCCGGGAAAAAATGCCGAACGTGCTTCTGCAAACGCTCGTCCGCGAAAC
CAACCTCTGGGGCTATCGTCCTTATCCTAAAAATATCATCGAACAGGTGATCGCCAAAGTCGATATCGATGTATGGCGCT
GTTTCTCGTTCCTCAACGACGTGCGCAACATGCGAACGGTAGCCGAAGTCGTTATGAAGCGGGGGCGCCTTTTCCAGCCA
GCCATATCCTTTACCCAGGCCGACTGGACAACGAACGGCTATTTTCTGAAACTCGTCGGGGAAATCGTCGATCTTTGCGG
CGGCACAAATGAAATCATTCTTTGCATCAAGGATATGGCGGGCGTTGGAAGCCCCCTGCGCATTCATTCACTCTTTGATG
CCATCAAACAGGCCTATCCCGATCTGGTTCTGCAATATCACCGACATGCCACCGACGGCCTTGCTCTTCCTGCCATACTT
GCCGCCGCACAGGCAGGAGCAAGCATCATCGATGTCGAAGAAGACTCCCTTACCCGTTTTTACGGCCAGGCCCCTCTTCT
GAGCGTAGCCTCCTTCCTCGAGGAATCGGGCATGAACGTCCATCTCAACACCAAAGAGGCGGATGAAGCCGTTCAGAAAG
TGCGCGACTGGATTCGCCACTACGAATGGGCAGAATCGCCGTTCAAAGGCTTCGATCATGGCGTCATCATGCACCGTATG
CCCGGCGGGGCCTTCCCAAGCTCCTTCGAACAGGCTCAAAAAGGTGGTTTCCTGCACCTGATGCCTGCGATTCTTAAAAT
GATGTCACTGTACAATCAGATCGTTAAATATTTTGACGTGACGCCCGGCTCACAGATCACATGGGTCACATGCAGCGGCA
TTGTGAACCATTACGACAAAGAGCGAGGCATGGCCGGCGTCAATCACGTCATCGATCTTCTGACACGATTTATCGAGGAG
AAAAAACAGGATTTCGATGCCATGCCTCCTGAAGATCAGCATGAACTTCTTCATCTTTTCAGGGGCGCACCGGGAGATTT
CAAAAATCTCATCGTCGGCAGTTACGGAAAACTGCCGATGGGATGGCCATCAGACTGGGTCTATACAAGCTGTTTCGGAG
ACGAGGGTCAGGAGAAAATCAGTCAGCGCCATGAAGACTCCCCGCTCGACGCCCTGCCGGACGAGGATTTAAAACGCCTG
CACCAGGAGCTCTCGGAAAAGCTTGACCGCACTCCGAGCGAAGAGGAGTTCGTTCTGTATCTCATGCATCCTAAAGACGC
CCTTGACTTCATCAAGTTCCGCGAAAAATACGGCGACACTCCGCTCGTTCTGCCAACCGATGTATGGAAATGTGGATTGC
AGAAAGCTGGTGCGAAAGTTGAGTTTGAGTATCGGGGCGTTCCTTATTCCATCGAGCGAGTCTCGATCGGTGCGGAACAT
GACGGCATTATTCACGCCGTCATGAAGGTTAACAATCAGATCCGGGTATTCAAAATCGAAACACCTCGCGCACGGAAAAC
CGAAATCAGAATAGCCAAAGGCCTTACCGATATTGGCGCTCCGATCAACGGAACCGTCTGGCGCATCGGAAATCCGGAAC
GCGGTCCAGTCAGGGCCGGCGACATTATTCACAAGGGCGAAGAGATCGCAAATCTCGAAGCCATGAAAATGGAAAACGCA
ATCTTCGCACCTTATAACGCTCAGATTACCGAAATCACGGTAAGACTTAACCAGATGGTTAAACAGGGTCAGCTTCTTTT
CGTTCTGGAAGAGATAAAGGAAAATGACTGA

Upstream 100 bases:

>100_bases
CCATGTTCCCGGAGGGCGACAGCGAAGAAGATGTGCTGAAAATCGCCAAATATATAGCTGAAATTTCTGCTCTTGGAACA
CCGAACTGGATTTAAGAACC

Downstream 100 bases:

>100_bases
CCGATAGCCGGGGGGCGACGGCAAATCGCCCCCTTCGTTCAACATGTCTGTCGCCAAACTGGCGGGTTTTTCGGAACCCG
CCGGAGTGCTGCGCTTTTCA

Product: biotin/lipoyl attachment domain-containing protein

Products: NA

Alternate protein names: Pyruvic carboxylase; PYC [H]

Number of amino acids: Translated: 676; Mature: 675

Protein sequence:

>676_residues
MTNSVFVKPGMSPKEIINNVRHLDAIALTSTGMRDAGQSDYKNRLRIRDLSTLSSNYNEMGLFSSECHGGARWHVGIMNR
RESPFEEIALLREKMPNVLLQTLVRETNLWGYRPYPKNIIEQVIAKVDIDVWRCFSFLNDVRNMRTVAEVVMKRGRLFQP
AISFTQADWTTNGYFLKLVGEIVDLCGGTNEIILCIKDMAGVGSPLRIHSLFDAIKQAYPDLVLQYHRHATDGLALPAIL
AAAQAGASIIDVEEDSLTRFYGQAPLLSVASFLEESGMNVHLNTKEADEAVQKVRDWIRHYEWAESPFKGFDHGVIMHRM
PGGAFPSSFEQAQKGGFLHLMPAILKMMSLYNQIVKYFDVTPGSQITWVTCSGIVNHYDKERGMAGVNHVIDLLTRFIEE
KKQDFDAMPPEDQHELLHLFRGAPGDFKNLIVGSYGKLPMGWPSDWVYTSCFGDEGQEKISQRHEDSPLDALPDEDLKRL
HQELSEKLDRTPSEEEFVLYLMHPKDALDFIKFREKYGDTPLVLPTDVWKCGLQKAGAKVEFEYRGVPYSIERVSIGAEH
DGIIHAVMKVNNQIRVFKIETPRARKTEIRIAKGLTDIGAPINGTVWRIGNPERGPVRAGDIIHKGEEIANLEAMKMENA
IFAPYNAQITEITVRLNQMVKQGQLLFVLEEIKEND

Sequences:

>Translated_676_residues
MTNSVFVKPGMSPKEIINNVRHLDAIALTSTGMRDAGQSDYKNRLRIRDLSTLSSNYNEMGLFSSECHGGARWHVGIMNR
RESPFEEIALLREKMPNVLLQTLVRETNLWGYRPYPKNIIEQVIAKVDIDVWRCFSFLNDVRNMRTVAEVVMKRGRLFQP
AISFTQADWTTNGYFLKLVGEIVDLCGGTNEIILCIKDMAGVGSPLRIHSLFDAIKQAYPDLVLQYHRHATDGLALPAIL
AAAQAGASIIDVEEDSLTRFYGQAPLLSVASFLEESGMNVHLNTKEADEAVQKVRDWIRHYEWAESPFKGFDHGVIMHRM
PGGAFPSSFEQAQKGGFLHLMPAILKMMSLYNQIVKYFDVTPGSQITWVTCSGIVNHYDKERGMAGVNHVIDLLTRFIEE
KKQDFDAMPPEDQHELLHLFRGAPGDFKNLIVGSYGKLPMGWPSDWVYTSCFGDEGQEKISQRHEDSPLDALPDEDLKRL
HQELSEKLDRTPSEEEFVLYLMHPKDALDFIKFREKYGDTPLVLPTDVWKCGLQKAGAKVEFEYRGVPYSIERVSIGAEH
DGIIHAVMKVNNQIRVFKIETPRARKTEIRIAKGLTDIGAPINGTVWRIGNPERGPVRAGDIIHKGEEIANLEAMKMENA
IFAPYNAQITEITVRLNQMVKQGQLLFVLEEIKEND
>Mature_675_residues
TNSVFVKPGMSPKEIINNVRHLDAIALTSTGMRDAGQSDYKNRLRIRDLSTLSSNYNEMGLFSSECHGGARWHVGIMNRR
ESPFEEIALLREKMPNVLLQTLVRETNLWGYRPYPKNIIEQVIAKVDIDVWRCFSFLNDVRNMRTVAEVVMKRGRLFQPA
ISFTQADWTTNGYFLKLVGEIVDLCGGTNEIILCIKDMAGVGSPLRIHSLFDAIKQAYPDLVLQYHRHATDGLALPAILA
AAQAGASIIDVEEDSLTRFYGQAPLLSVASFLEESGMNVHLNTKEADEAVQKVRDWIRHYEWAESPFKGFDHGVIMHRMP
GGAFPSSFEQAQKGGFLHLMPAILKMMSLYNQIVKYFDVTPGSQITWVTCSGIVNHYDKERGMAGVNHVIDLLTRFIEEK
KQDFDAMPPEDQHELLHLFRGAPGDFKNLIVGSYGKLPMGWPSDWVYTSCFGDEGQEKISQRHEDSPLDALPDEDLKRLH
QELSEKLDRTPSEEEFVLYLMHPKDALDFIKFREKYGDTPLVLPTDVWKCGLQKAGAKVEFEYRGVPYSIERVSIGAEHD
GIIHAVMKVNNQIRVFKIETPRARKTEIRIAKGLTDIGAPINGTVWRIGNPERGPVRAGDIIHKGEEIANLEAMKMENAI
FAPYNAQITEITVRLNQMVKQGQLLFVLEEIKEND

Specific function: Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second, leading to oxaloacetate production. Fulfills an anaplerotic functi

COG id: COG1038

COG function: function code C; Pyruvate carboxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=689, Percent_Identity=27.1407837445573, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI106049295, Length=689, Percent_Identity=27.1407837445573, Blast_Score=182, Evalue=1e-45,
Organism=Homo sapiens, GI106049292, Length=689, Percent_Identity=27.1407837445573, Blast_Score=182, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17562816, Length=677, Percent_Identity=27.178729689808, Blast_Score=189, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319695, Length=688, Percent_Identity=28.0523255813953, Blast_Score=200, Evalue=8e-52,
Organism=Saccharomyces cerevisiae, GI6321376, Length=685, Percent_Identity=27.5912408759124, Blast_Score=199, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24652212, Length=681, Percent_Identity=25.697503671072, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24652210, Length=681, Percent_Identity=25.697503671072, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24652214, Length=681, Percent_Identity=25.697503671072, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI19921944, Length=681, Percent_Identity=25.697503671072, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24652216, Length=681, Percent_Identity=25.697503671072, Blast_Score=192, Evalue=9e-49,
Organism=Drosophila melanogaster, GI281363050, Length=681, Percent_Identity=25.697503671072, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24652224, Length=681, Percent_Identity=25.697503671072, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24652222, Length=681, Percent_Identity=25.697503671072, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24652220, Length=681, Percent_Identity=25.697503671072, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24652218, Length=681, Percent_Identity=25.697503671072, Blast_Score=191, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR000089
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR003379
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR000891
- InterPro:   IPR005930
- InterPro:   IPR011054
- InterPro:   IPR011053 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00364 Biotin_lipoyl; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 76519; Mature: 76387

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSVFVKPGMSPKEIINNVRHLDAIALTSTGMRDAGQSDYKNRLRIRDLSTLSSNYNEM
CCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEHHHHHHHHCCCCCC
GLFSSECHGGARWHVGIMNRRESPFEEIALLREKMPNVLLQTLVRETNLWGYRPYPKNII
CCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
EQVIAKVDIDVWRCFSFLNDVRNMRTVAEVVMKRGRLFQPAISFTQADWTTNGYFLKLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHCCCCCCCCHHHHHHH
EIVDLCGGTNEIILCIKDMAGVGSPLRIHSLFDAIKQAYPDLVLQYHRHATDGLALPAIL
HHHHHHCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AAAQAGASIIDVEEDSLTRFYGQAPLLSVASFLEESGMNVHLNTKEADEAVQKVRDWIRH
HHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
YEWAESPFKGFDHGVIMHRMPGGAFPSSFEQAQKGGFLHLMPAILKMMSLYNQIVKYFDV
HHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
TPGSQITWVTCSGIVNHYDKERGMAGVNHVIDLLTRFIEEKKQDFDAMPPEDQHELLHLF
CCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RGAPGDFKNLIVGSYGKLPMGWPSDWVYTSCFGDEGQEKISQRHEDSPLDALPDEDLKRL
CCCCCCHHHHEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
HQELSEKLDRTPSEEEFVLYLMHPKDALDFIKFREKYGDTPLVLPTDVWKCGLQKAGAKV
HHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCEE
EFEYRGVPYSIERVSIGAEHDGIIHAVMKVNNQIRVFKIETPRARKTEIRIAKGLTDIGA
EEEECCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCHHHHCC
PINGTVWRIGNPERGPVRAGDIIHKGEEIANLEAMKMENAIFAPYNAQITEITVRLNQMV
CCCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
KQGQLLFVLEEIKEND
HCCCCEEEEHHHCCCC
>Mature Secondary Structure 
TNSVFVKPGMSPKEIINNVRHLDAIALTSTGMRDAGQSDYKNRLRIRDLSTLSSNYNEM
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCEEHHHHHHHHCCCCCC
GLFSSECHGGARWHVGIMNRRESPFEEIALLREKMPNVLLQTLVRETNLWGYRPYPKNII
CCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
EQVIAKVDIDVWRCFSFLNDVRNMRTVAEVVMKRGRLFQPAISFTQADWTTNGYFLKLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHHHHCCCCCCCCHHHHHHH
EIVDLCGGTNEIILCIKDMAGVGSPLRIHSLFDAIKQAYPDLVLQYHRHATDGLALPAIL
HHHHHHCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
AAAQAGASIIDVEEDSLTRFYGQAPLLSVASFLEESGMNVHLNTKEADEAVQKVRDWIRH
HHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
YEWAESPFKGFDHGVIMHRMPGGAFPSSFEQAQKGGFLHLMPAILKMMSLYNQIVKYFDV
HHHCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
TPGSQITWVTCSGIVNHYDKERGMAGVNHVIDLLTRFIEEKKQDFDAMPPEDQHELLHLF
CCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RGAPGDFKNLIVGSYGKLPMGWPSDWVYTSCFGDEGQEKISQRHEDSPLDALPDEDLKRL
CCCCCCHHHHEECCCCCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
HQELSEKLDRTPSEEEFVLYLMHPKDALDFIKFREKYGDTPLVLPTDVWKCGLQKAGAKV
HHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHCCCEE
EFEYRGVPYSIERVSIGAEHDGIIHAVMKVNNQIRVFKIETPRARKTEIRIAKGLTDIGA
EEEECCCCEEEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCHHHHCC
PINGTVWRIGNPERGPVRAGDIIHKGEEIANLEAMKMENAIFAPYNAQITEITVRLNQMV
CCCCEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHH
KQGQLLFVLEEIKEND
HCCCCEEEEHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]