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The map label for this gene is queA

Identifier: 119356633

GI number: 119356633

Start: 913777

End: 914802

Strand: Direct

Name: queA

Synonym: Cpha266_0802

Alternate gene names: 119356633

Gene position: 913777-914802 (Clockwise)

Preceding gene: 119356632

Following gene: 119356634

Centisome position: 29.16

GC content: 50.0

Gene sequence:

>1026_bases
ATGAAGGTCAGTGATTTCGATTATGAACTTCCGGAGTCGCACATTGCCCGGTATCCTCCGGATGAGAGGGGCTCAACCAG
ACTGCTTGTACTGCAAAGATCGACAGGCGAAATTATGCACAGTCGCTATGCCGGGCTGCACGGCTTTTTGCGAAAGGGCG
ATCTTCTTGTGCTCAACAACAGCAGGGTAGTCAAGGCGAGGATGTTCGCCTTTAAGCCTACGGGAGGAAAGATTGAGCTT
GTGCTGCTCGAAAAACATGGAGATGAGCAGAACCTTGTCCTTTACAGGGGAAGCCTTAAAAAAGGAGATGCGCTTCTTGC
TTATGGTTGTGAATTTGGTGTTGAAGAGCTGGTCGGGCAGGGCGTTGCCGTGCTTTCAGTAAAGGGCGAGGGGACGGTTC
AGGATGTTTTTGAGCGTTATGCCGCGATGCCTATTCCTCCCTACCTGAAACGGGAGGCTGAGGAGATTGATCGTGAACGC
TATCAGACCGTTTTTGCTGAACAGCCCGGATCGGTTGCCGCTCCTACGGCTTCACTCAATATGACCGGCGAACTGTTTGA
CTCGCTTCGGCAAAAAGGAGTATCGATCGCCTCTATGACCCTGCATGTCGGCCTTGGAACTTTTCTGCCAATCCGAGTCG
ATCATTTTGATGAGCATGTCATGCATCGGGAGTTTTATGTGATTCCTGATGAAACGATCGCCCTGATTCGAACAACAAAA
AATAATGGAGGGCGAGTAATCGCTGTAGGGACAACGGTTACTCGGGCGCTTGAACATGCGGCAGACGTTGTTTTCAACAG
CAACGGTAACGGATCGGTATCCGGCGAAGCCGACATTTTTATCTATCCGGGATACCGGTTTCGGGTTATTGACGCTCTTC
TGACAAATTTTCATGCTCCGCGCTCGACGGTACTGATGCTGACAGCGGCTTTTGCCGGTGCAGAAAAGCTTTTTCATGCT
TATGCTGAGGCTCTTCGTTGGGAGTATGATTTTTTGAGCTATGGTGACAGCATGCTTATTCTTTAA

Upstream 100 bases:

>100_bases
AAAAACAGCTTCAGGAATGTGGTAAAAAAATTGCCGCATTTGCCGATTATGAGGGTCTTCAGGCTCATGCTGAGGCTGTC
CGCGTAAGACTTGATTCGTG

Downstream 100 bases:

>100_bases
GCGTTGCCCAACGATAGGTATGCTGTTCTGTCAGTCCCTTGCCGCTATCCTTTGAATGAACTCCTTTTCGACAAGTCGGG
AGATGTTGCGCCTGATGGAT

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MKVSDFDYELPESHIARYPPDERGSTRLLVLQRSTGEIMHSRYAGLHGFLRKGDLLVLNNSRVVKARMFAFKPTGGKIEL
VLLEKHGDEQNLVLYRGSLKKGDALLAYGCEFGVEELVGQGVAVLSVKGEGTVQDVFERYAAMPIPPYLKREAEEIDRER
YQTVFAEQPGSVAAPTASLNMTGELFDSLRQKGVSIASMTLHVGLGTFLPIRVDHFDEHVMHREFYVIPDETIALIRTTK
NNGGRVIAVGTTVTRALEHAADVVFNSNGNGSVSGEADIFIYPGYRFRVIDALLTNFHAPRSTVLMLTAAFAGAEKLFHA
YAEALRWEYDFLSYGDSMLIL

Sequences:

>Translated_341_residues
MKVSDFDYELPESHIARYPPDERGSTRLLVLQRSTGEIMHSRYAGLHGFLRKGDLLVLNNSRVVKARMFAFKPTGGKIEL
VLLEKHGDEQNLVLYRGSLKKGDALLAYGCEFGVEELVGQGVAVLSVKGEGTVQDVFERYAAMPIPPYLKREAEEIDRER
YQTVFAEQPGSVAAPTASLNMTGELFDSLRQKGVSIASMTLHVGLGTFLPIRVDHFDEHVMHREFYVIPDETIALIRTTK
NNGGRVIAVGTTVTRALEHAADVVFNSNGNGSVSGEADIFIYPGYRFRVIDALLTNFHAPRSTVLMLTAAFAGAEKLFHA
YAEALRWEYDFLSYGDSMLIL
>Mature_341_residues
MKVSDFDYELPESHIARYPPDERGSTRLLVLQRSTGEIMHSRYAGLHGFLRKGDLLVLNNSRVVKARMFAFKPTGGKIEL
VLLEKHGDEQNLVLYRGSLKKGDALLAYGCEFGVEELVGQGVAVLSVKGEGTVQDVFERYAAMPIPPYLKREAEEIDRER
YQTVFAEQPGSVAAPTASLNMTGELFDSLRQKGVSIASMTLHVGLGTFLPIRVDHFDEHVMHREFYVIPDETIALIRTTK
NNGGRVIAVGTTVTRALEHAADVVFNSNGNGSVSGEADIFIYPGYRFRVIDALLTNFHAPRSTVLMLTAAFAGAEKLFHA
YAEALRWEYDFLSYGDSMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=345, Percent_Identity=45.5072463768116, Blast_Score=286, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CHLPD (A1BEM6)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911277.1
- ProteinModelPortal:   A1BEM6
- SMR:   A1BEM6
- STRING:   A1BEM6
- GeneID:   4570221
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0802
- NMPDR:   fig|290317.7.peg.849
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   CLSK637367
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37881; Mature: 37881

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSDFDYELPESHIARYPPDERGSTRLLVLQRSTGEIMHSRYAGLHGFLRKGDLLVLNN
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECC
SRVVKARMFAFKPTGGKIELVLLEKHGDEQNLVLYRGSLKKGDALLAYGCEFGVEELVGQ
CEEEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHCC
GVAVLSVKGEGTVQDVFERYAAMPIPPYLKREAEEIDRERYQTVFAEQPGSVAAPTASLN
CEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
MTGELFDSLRQKGVSIASMTLHVGLGTFLPIRVDHFDEHVMHREFYVIPDETIALIRTTK
CCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCHHHHHHCCEEEEECCCEEEEEEEEC
NNGGRVIAVGTTVTRALEHAADVVFNSNGNGSVSGEADIFIYPGYRFRVIDALLTNFHAP
CCCCEEEEECHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCC
RSTVLMLTAAFAGAEKLFHAYAEALRWEYDFLSYGDSMLIL
CHHEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEC
>Mature Secondary Structure
MKVSDFDYELPESHIARYPPDERGSTRLLVLQRSTGEIMHSRYAGLHGFLRKGDLLVLNN
CCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECC
SRVVKARMFAFKPTGGKIELVLLEKHGDEQNLVLYRGSLKKGDALLAYGCEFGVEELVGQ
CEEEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHCC
GVAVLSVKGEGTVQDVFERYAAMPIPPYLKREAEEIDRERYQTVFAEQPGSVAAPTASLN
CEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECC
MTGELFDSLRQKGVSIASMTLHVGLGTFLPIRVDHFDEHVMHREFYVIPDETIALIRTTK
CCHHHHHHHHHCCCEEEEEEEEECCCCEEEEEECCHHHHHHCCEEEEECCCEEEEEEEEC
NNGGRVIAVGTTVTRALEHAADVVFNSNGNGSVSGEADIFIYPGYRFRVIDALLTNFHAP
CCCCEEEEECHHHHHHHHHHHHEEEECCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCC
RSTVLMLTAAFAGAEKLFHAYAEALRWEYDFLSYGDSMLIL
CHHEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA