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The map label for this gene is odhL [H]

Identifier: 119356560

GI number: 119356560

Start: 827746

End: 828450

Strand: Reverse

Name: odhL [H]

Synonym: Cpha266_0726

Alternate gene names: 119356560

Gene position: 828450-827746 (Counterclockwise)

Preceding gene: 119356561

Following gene: 119356553

Centisome position: 26.44

GC content: 52.91

Gene sequence:

>705_bases
TTGAAAAAAAGCTCTCAAGGACATGAAAAAACAACCGAAAAGGGAGTCAGGGTAACCTATGCCGGCGACCAGGGCTCTGA
ACATGTACTTGAATGCGACAAGCTGATTGTTTCTGTTGGTCGGATACCTGACACCGAAAAACTCAACCTTGCGGGCATCG
GGCTGCAAATCGACGAACGGGGATTTATTCCTGTAAACGACCATTGCGCTACTGTTGCGGAAGGTGTATACGCTATCGGG
GATGTTGTACGGGGCCCGATGCTGGCGCACAAGGCTGAAGACGAGGGCGTCATGGTCGCAGAACTCATCGCCGGCCAGAA
CCCTCGTCTTGATTATAACAGCATGCCCCGGGTTATCTACACAACCCCTGAAATTGCCTGGGCAGGAAAAACCGAACAGC
AACTCAGAGCGGAGGGACGTGACTACAAAACAGGTCAGTTTCCCTTTGCGGCCAACGGTCGGGCACTGGGCCCTGGCGAT
GCTGAAGGGTTTATAAAAATGCTTGCCGACGCCAAAACCGACGAAATCCTCGGCGTGCATATTATCGGAGCAAATGCTTC
AGAACTGATTGCCGAAGCAGCTCTGGCCCTGGAGTTCAGGGCTGCGGCCGAAGACATCGCTCGAACATGCCATCCTCATC
CGAGTCTCTCGGAAGTCATGCGAGAAGCCGCGCTTGCTCTTGAAAAACGGGCATTGAATATATAG

Upstream 100 bases:

>100_bases
GTTATAGTAAAGGAACCCGCTCTCCTGCCCCACAGCCTGATCGCCATCGGTTGTTACCCCGGCCACCCTCTTTCCGGCAA
AACATACCGGTTTAATCTTT

Downstream 100 bases:

>100_bases
GATATCGAACATCGGCACCTCTTTGAAACTCACCTGACGAATGAAGTTCAGAGAGGTGCGCAAGGCATCAGCAAATCCGT
GGAAGCTGCTCCCCAAGGGG

Product: pyridine nucleotide-disulfide oxidoreductase dimerisation region

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKKSSQGHEKTTEKGVRVTYAGDQGSEHVLECDKLIVSVGRIPDTEKLNLAGIGLQIDERGFIPVNDHCATVAEGVYAIG
DVVRGPMLAHKAEDEGVMVAELIAGQNPRLDYNSMPRVIYTTPEIAWAGKTEQQLRAEGRDYKTGQFPFAANGRALGPGD
AEGFIKMLADAKTDEILGVHIIGANASELIAEAALALEFRAAAEDIARTCHPHPSLSEVMREAALALEKRALNI

Sequences:

>Translated_234_residues
MKKSSQGHEKTTEKGVRVTYAGDQGSEHVLECDKLIVSVGRIPDTEKLNLAGIGLQIDERGFIPVNDHCATVAEGVYAIG
DVVRGPMLAHKAEDEGVMVAELIAGQNPRLDYNSMPRVIYTTPEIAWAGKTEQQLRAEGRDYKTGQFPFAANGRALGPGD
AEGFIKMLADAKTDEILGVHIIGANASELIAEAALALEFRAAAEDIARTCHPHPSLSEVMREAALALEKRALNI
>Mature_234_residues
MKKSSQGHEKTTEKGVRVTYAGDQGSEHVLECDKLIVSVGRIPDTEKLNLAGIGLQIDERGFIPVNDHCATVAEGVYAIG
DVVRGPMLAHKAEDEGVMVAELIAGQNPRLDYNSMPRVIYTTPEIAWAGKTEQQLRAEGRDYKTGQFPFAANGRALGPGD
AEGFIKMLADAKTDEILGVHIIGANASELIAEAALALEFRAAAEDIARTCHPHPSLSEVMREAALALEKRALNI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=206, Percent_Identity=54.3689320388349, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI50301238, Length=192, Percent_Identity=29.6875, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI22035672, Length=221, Percent_Identity=30.316742081448, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI148277071, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI33519430, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33519428, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI33519426, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI148277065, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI291045266, Length=225, Percent_Identity=27.1111111111111, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786307, Length=216, Percent_Identity=39.8148148148148, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI87082354, Length=200, Percent_Identity=37.5, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI87081717, Length=200, Percent_Identity=29.5, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1789915, Length=200, Percent_Identity=29.5, Blast_Score=88, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI32565766, Length=202, Percent_Identity=58.4158415841584, Blast_Score=255, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17557007, Length=213, Percent_Identity=29.1079812206573, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321091, Length=204, Percent_Identity=50.4901960784314, Blast_Score=219, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6325240, Length=207, Percent_Identity=36.231884057971, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21358499, Length=203, Percent_Identity=56.1576354679803, Blast_Score=239, Evalue=8e-64,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 25171; Mature: 25171

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSSQGHEKTTEKGVRVTYAGDQGSEHVLECDKLIVSVGRIPDTEKLNLAGIGLQIDER
CCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCC
GFIPVNDHCATVAEGVYAIGDVVRGPMLAHKAEDEGVMVAELIAGQNPRLDYNSMPRVIY
CCEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCEEEE
TTPEIAWAGKTEQQLRAEGRDYKTGQFPFAANGRALGPGDAEGFIKMLADAKTDEILGVH
ECCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
IIGANASELIAEAALALEFRAAAEDIARTCHPHPSLSEVMREAALALEKRALNI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKSSQGHEKTTEKGVRVTYAGDQGSEHVLECDKLIVSVGRIPDTEKLNLAGIGLQIDER
CCCCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEECCC
GFIPVNDHCATVAEGVYAIGDVVRGPMLAHKAEDEGVMVAELIAGQNPRLDYNSMPRVIY
CCEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEEEEHHHCCCCCCCCCCCCCEEEE
TTPEIAWAGKTEQQLRAEGRDYKTGQFPFAANGRALGPGDAEGFIKMLADAKTDEILGVH
ECCCEEECCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEE
IIGANASELIAEAALALEFRAAAEDIARTCHPHPSLSEVMREAALALEKRALNI
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]