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The map label for this gene is pyrB

Identifier: 119356553

GI number: 119356553

Start: 820575

End: 821501

Strand: Reverse

Name: pyrB

Synonym: Cpha266_0719

Alternate gene names: 119356553

Gene position: 821501-820575 (Counterclockwise)

Preceding gene: 119356560

Following gene: 119356552

Centisome position: 26.21

GC content: 52.21

Gene sequence:

>927_bases
GTGAAGCATCTTACCGGTTTATGTGAACTCCCTCTCTATGAAATCCAGCGTCTGCTTGATCTGGCCGCCGTTTTTAAAAA
AGAGCTGAGAACAAAAGCTCCCGCTTTTGCGCCGACACTGCTGTCAAAAAAAATTGCCCTCGCTTTTTTCGAAAACTCGA
CCCGAACCCGATTCTCCTTTGAAATCGCAGCAAAACACCTTGGAGCAAGCACGCTTAACTTTACCGCATCGTCAAGCAGC
GTCAGCAAAGGAGAAACGCTCGGCGACACCATCAAAAACCTCGAAGCCATGCAGGTGGATGCCTTTGTTATCAGGCATCC
CTCCTCAGGCGCAGCCGACCTTATTACGGGAATAACAAGCAAAAGCGTCATTAATGCGGGAGATGGATCGCATGAGCATC
CAACCCAGGCACTGCTCGACATGTTTACGCTCAAAGAACACTTCGGCAGTCTGGAGGGATTGAACGTCTTCATTCTTGGC
GATATTCTGCACAGCCGTGTTGCCCGATCAAATATTTTCGGCATGCTCACCCTTGGAGCAAACGTTGCGCTATGCAGCCC
GTCAACCCTTCTTCCTCCCGGCACCTCTGACCTCGGAATCCGCATTTTCACGGATCTTGATCAGGCAATCCAGTGGGCAG
ATGCGGCAATCGTGCTCCGTCTGCAACTTGAGCGCGCCACGGGCGGCTATCTTCCTTCGCTTGGAGAATATGCCGTACAC
TTCGGGCTTACCGACGAACGGCTCGAACGGATTCGCAAACATCTCCTGGTACTGCATCCCGGCCCGATAAACCGCGAAAT
CGAAATATCAAGCCGCGTTGCAGACCGTATGCAACCCCCCGGATTCTCAAGCAGTCTCCTGCTTCAACAGGTAACCAATG
GCATTGCAGTCCGAATGGCTGTTCTGCAAACGCTGCTTGCAGAGTGA

Upstream 100 bases:

>100_bases
ATCTGAAGTATCAGCGCAGGTAATGTAGTTCCTGATCAACAATTTTTTTGTACCTTTCAGCAAAATTCAATCCTTGAAAC
CGAGCAAAGCACGATCTCTG

Downstream 100 bases:

>100_bases
TTTTTAATTTTTTCATCAAACCAAACCAAAACAGAACGGTCATGAAACACAAAAGCATGATATGCCGGCTGCTCACCGTA
ACTGCAGCGCTCACCATCTC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKHLTGLCELPLYEIQRLLDLAAVFKKELRTKAPAFAPTLLSKKIALAFFENSTRTRFSFEIAAKHLGASTLNFTASSSS
VSKGETLGDTIKNLEAMQVDAFVIRHPSSGAADLITGITSKSVINAGDGSHEHPTQALLDMFTLKEHFGSLEGLNVFILG
DILHSRVARSNIFGMLTLGANVALCSPSTLLPPGTSDLGIRIFTDLDQAIQWADAAIVLRLQLERATGGYLPSLGEYAVH
FGLTDERLERIRKHLLVLHPGPINREIEISSRVADRMQPPGFSSSLLLQQVTNGIAVRMAVLQTLLAE

Sequences:

>Translated_308_residues
MKHLTGLCELPLYEIQRLLDLAAVFKKELRTKAPAFAPTLLSKKIALAFFENSTRTRFSFEIAAKHLGASTLNFTASSSS
VSKGETLGDTIKNLEAMQVDAFVIRHPSSGAADLITGITSKSVINAGDGSHEHPTQALLDMFTLKEHFGSLEGLNVFILG
DILHSRVARSNIFGMLTLGANVALCSPSTLLPPGTSDLGIRIFTDLDQAIQWADAAIVLRLQLERATGGYLPSLGEYAVH
FGLTDERLERIRKHLLVLHPGPINREIEISSRVADRMQPPGFSSSLLLQQVTNGIAVRMAVLQTLLAE
>Mature_308_residues
MKHLTGLCELPLYEIQRLLDLAAVFKKELRTKAPAFAPTLLSKKIALAFFENSTRTRFSFEIAAKHLGASTLNFTASSSS
VSKGETLGDTIKNLEAMQVDAFVIRHPSSGAADLITGITSKSVINAGDGSHEHPTQALLDMFTLKEHFGSLEGLNVFILG
DILHSRVARSNIFGMLTLGANVALCSPSTLLPPGTSDLGIRIFTDLDQAIQWADAAIVLRLQLERATGGYLPSLGEYAVH
FGLTDERLERIRKHLLVLHPGPINREIEISSRVADRMQPPGFSSSLLLQQVTNGIAVRMAVLQTLLAE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=280, Percent_Identity=35, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI38788445, Length=165, Percent_Identity=34.5454545454545, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367364, Length=320, Percent_Identity=34.6875, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1790703, Length=193, Percent_Identity=30.0518134715026, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=317, Percent_Identity=32.1766561514196, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6322331, Length=278, Percent_Identity=32.3741007194245, Blast_Score=122, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24642586, Length=324, Percent_Identity=33.9506172839506, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_CHLPD (A1BEE6)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911197.1
- ProteinModelPortal:   A1BEE6
- SMR:   A1BEE6
- STRING:   A1BEE6
- GeneID:   4569893
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0719
- NMPDR:   fig|290317.7.peg.756
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33452; Mature: 33452

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLTGLCELPLYEIQRLLDLAAVFKKELRTKAPAFAPTLLSKKIALAFFENSTRTRFSF
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEEH
EIAAKHLGASTLNFTASSSSVSKGETLGDTIKNLEAMQVDAFVIRHPSSGAADLITGITS
HHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCC
KSVINAGDGSHEHPTQALLDMFTLKEHFGSLEGLNVFILGDILHSRVARSNIFGMLTLGA
CCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHEEEEEECC
NVALCSPSTLLPPGTSDLGIRIFTDLDQAIQWADAAIVLRLQLERATGGYLPSLGEYAVH
EEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHEEEEEEEEECCCCCCCCCCCHHHEE
FGLTDERLERIRKHLLVLHPGPINREIEISSRVADRMQPPGFSSSLLLQQVTNGIAVRMA
CCCCHHHHHHHHHHEEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VLQTLLAE
HHHHHHCC
>Mature Secondary Structure
MKHLTGLCELPLYEIQRLLDLAAVFKKELRTKAPAFAPTLLSKKIALAFFENSTRTRFSF
CCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEECCCCCEEEEH
EIAAKHLGASTLNFTASSSSVSKGETLGDTIKNLEAMQVDAFVIRHPSSGAADLITGITS
HHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHHHCCC
KSVINAGDGSHEHPTQALLDMFTLKEHFGSLEGLNVFILGDILHSRVARSNIFGMLTLGA
CCEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHEEEEEECC
NVALCSPSTLLPPGTSDLGIRIFTDLDQAIQWADAAIVLRLQLERATGGYLPSLGEYAVH
EEEEECCCCCCCCCCCCCCEEEEECHHHHHHHHHHEEEEEEEEECCCCCCCCCCCHHHEE
FGLTDERLERIRKHLLVLHPGPINREIEISSRVADRMQPPGFSSSLLLQQVTNGIAVRMA
CCCCHHHHHHHHHHEEEECCCCCCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
VLQTLLAE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA