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The map label for this gene is purH [H]

Identifier: 119356492

GI number: 119356492

Start: 748982

End: 750547

Strand: Direct

Name: purH [H]

Synonym: Cpha266_0657

Alternate gene names: 119356492

Gene position: 748982-750547 (Clockwise)

Preceding gene: 119356489

Following gene: 119356494

Centisome position: 23.9

GC content: 50.26

Gene sequence:

>1566_bases
ATGTCTGATCCTGTCATCAAACGGGCGCTGGTCTCTGTTTCCGACAAAACCGGTATTGTGGATTTCTGCCGGGAGCTTTC
GCTTCTCGGCGTTGAGGTGTTTTCAACGGGCGGAACCCTGAAGACTCTTCAGGATGCCGGAATAGCTGCGGCTTCTATTT
CGACCATCACCGGATTTCCGGAAATTATGGATGGGCGGGTCAAAACCCTCCATCCTAAAATACATGGAGGACTGCTCGCC
GTAAGGGAAAATCCTGATCATGTCAACCAGGCGAACGAAAACGGGATCAGCTTTATTGATCTTGTTGTTGTTAACCTTTA
TCCATTCGAGGCCACAGTTGCAAAACCGGACGTGACCTTCGAGGATGCCATAGAAAATATCGATATCGGCGGTCCCTCCA
TGCTTCGCAGTGCTGCCAAGAACAACGAATCGGTAACAGTGGTCACGGATAGCGCCGACTATGCGCTTGTGTTGCAGGAG
ATGCGTAATAATAACGGTGCGACGAAAAGGGAGACCCGGCTGGCGCTTGCTCTGAAGGTTTTTGAACTTACCTCTCGTTA
TGATCGCGCAATCGCCTCTTATCTTGCAGGAGCTCAGCATGAAGCAGATTCTTCCATGACGGTAAAACTTGAACGTGAGC
TCGATATGCGCTATGGCGAAAATCCTCATCAGAGCGCTGGGCTTTACCGCCTGACTGATGAGAACGGAACGCGTTCTTTT
AGCGATTATTTCGAGAAACTGCATGGCAAGGAGCTCTCTTACAACAATATGCTCGATATTGCCGCCGCAGTCTCCCTTAT
TGAGGAGTTCCGTGGTGAAGAGCCGACAGTAGTCATTATCAAACATACAAACCCCTGCGGTGTTGCGCAGGCCCCGACAC
TTGCCGAAGCATACCGGAGAGCATTCTCAACCGATACCCAGGCCCCTTTTGGCGGCATTATTGCCTTTAACCATCCTCTC
GACATGGAAGCGGCAACGGCGGTCAATGAGATTTTTACCGAGATTCTTATTGCTCCGGCATTTGAGGATGGCGTGCTTGA
GATGCTGATGAAGAAAAAAGATCGCAGGCTTGTGCGGCAGACGAGTGCCCTGCCCAAAGGTGGTTGGGAGTTCAAGTCTA
CTCCGTTCGGGATGCTTGTTCAGGAACGTGACAGCAAAATCGTCACAAAAGAGGATCTGACTGTTGTGACCAAACGGCAG
CCAACAGAAGAGGAGGTTGCAGACATGATGTTTGCCTGGAAAATCTGCAAGCACATCAAGTCAAACACGATTCTTTATGT
TAAAAATCGCCAGACCTTTGGAGTTGGTGCCGGTCAGATGTCCCGTGTTGACTCTTCAAAAATCGCGCGTTGGAAAGCTT
CTGAAGTCAATCTCGATCTGCATGGCTCGGTGGTTGCTTCAGATGCGTTTTTCCCGTTTGCCGATGGTCTTCTTGCCGCA
GCAGAAGCAGGCGTTACCGCAGTTATTCAGCCAGGCGGTTCGATCAGGGATAACGAGGTGATTGAAGCGGCAGACGCTAA
CAATCTTGCCATGGTTTTTACAGGAATGCGCCACTTCAAACACTGA

Upstream 100 bases:

>100_bases
GCTGTTTATTGCGTATTGTTGTCGCCTGAAATAATTGGTGATATTTTCTTAAATTCCGGCTTTAGAATCTGCGACTTAAC
GACAAATAAACTCTTTGCAA

Downstream 100 bases:

>100_bases
GGGCTTTCTCGATCAATACGTGAGTAATTCTGAAAAAAGGCGTACCGATGGTGCGCCTTTTTTTGTTGCTGCTTTATGGT
TTTATTTCGGTTCTGTTCAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSDPVIKRALVSVSDKTGIVDFCRELSLLGVEVFSTGGTLKTLQDAGIAAASISTITGFPEIMDGRVKTLHPKIHGGLLA
VRENPDHVNQANENGISFIDLVVVNLYPFEATVAKPDVTFEDAIENIDIGGPSMLRSAAKNNESVTVVTDSADYALVLQE
MRNNNGATKRETRLALALKVFELTSRYDRAIASYLAGAQHEADSSMTVKLERELDMRYGENPHQSAGLYRLTDENGTRSF
SDYFEKLHGKELSYNNMLDIAAAVSLIEEFRGEEPTVVIIKHTNPCGVAQAPTLAEAYRRAFSTDTQAPFGGIIAFNHPL
DMEAATAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVRQTSALPKGGWEFKSTPFGMLVQERDSKIVTKEDLTVVTKRQ
PTEEEVADMMFAWKICKHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVNLDLHGSVVASDAFFPFADGLLAA
AEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH

Sequences:

>Translated_521_residues
MSDPVIKRALVSVSDKTGIVDFCRELSLLGVEVFSTGGTLKTLQDAGIAAASISTITGFPEIMDGRVKTLHPKIHGGLLA
VRENPDHVNQANENGISFIDLVVVNLYPFEATVAKPDVTFEDAIENIDIGGPSMLRSAAKNNESVTVVTDSADYALVLQE
MRNNNGATKRETRLALALKVFELTSRYDRAIASYLAGAQHEADSSMTVKLERELDMRYGENPHQSAGLYRLTDENGTRSF
SDYFEKLHGKELSYNNMLDIAAAVSLIEEFRGEEPTVVIIKHTNPCGVAQAPTLAEAYRRAFSTDTQAPFGGIIAFNHPL
DMEAATAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVRQTSALPKGGWEFKSTPFGMLVQERDSKIVTKEDLTVVTKRQ
PTEEEVADMMFAWKICKHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVNLDLHGSVVASDAFFPFADGLLAA
AEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
>Mature_520_residues
SDPVIKRALVSVSDKTGIVDFCRELSLLGVEVFSTGGTLKTLQDAGIAAASISTITGFPEIMDGRVKTLHPKIHGGLLAV
RENPDHVNQANENGISFIDLVVVNLYPFEATVAKPDVTFEDAIENIDIGGPSMLRSAAKNNESVTVVTDSADYALVLQEM
RNNNGATKRETRLALALKVFELTSRYDRAIASYLAGAQHEADSSMTVKLERELDMRYGENPHQSAGLYRLTDENGTRSFS
DYFEKLHGKELSYNNMLDIAAAVSLIEEFRGEEPTVVIIKHTNPCGVAQAPTLAEAYRRAFSTDTQAPFGGIIAFNHPLD
MEAATAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVRQTSALPKGGWEFKSTPFGMLVQERDSKIVTKEDLTVVTKRQP
TEEEVADMMFAWKICKHIKSNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVNLDLHGSVVASDAFFPFADGLLAAA
EAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=492, Percent_Identity=36.7886178861789, Blast_Score=265, Evalue=8e-71,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=49.9058380414313, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71985564, Length=516, Percent_Identity=32.9457364341085, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI71985574, Length=346, Percent_Identity=26.878612716763, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323056, Length=484, Percent_Identity=33.4710743801653, Blast_Score=239, Evalue=7e-64,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=33.6082474226804, Blast_Score=239, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24649832, Length=489, Percent_Identity=36.8098159509202, Blast_Score=269, Evalue=3e-72,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57053; Mature: 56922

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPVIKRALVSVSDKTGIVDFCRELSLLGVEVFSTGGTLKTLQDAGIAAASISTITGFP
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCHHHHHHCCCCHHHHHHHCCCH
EIMDGRVKTLHPKIHGGLLAVRENPDHVNQANENGISFIDLVVVNLYPFEATVAKPDVTF
HHHCCCCEEECCCCCCCEEEEECCCCHHCCCCCCCCEEEEEEEEEEECCEEEECCCCCCH
EDAIENIDIGGPSMLRSAAKNNESVTVVTDSADYALVLQEMRNNNGATKRETRLALALKV
HHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
FELTSRYDRAIASYLAGAQHEADSSMTVKLERELDMRYGENPHQSAGLYRLTDENGTRSF
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCEEEEECCCCCCHH
SDYFEKLHGKELSYNNMLDIAAAVSLIEEFRGEEPTVVIIKHTNPCGVAQAPTLAEAYRR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AFSTDTQAPFGGIIAFNHPLDMEAATAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVRQ
HHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TSALPKGGWEFKSTPFGMLVQERDSKIVTKEDLTVVTKRQPTEEEVADMMFAWKICKHIK
HHCCCCCCCCCCCCCCCCEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHC
SNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVNLDLHGSVVASDAFFPFADGLLAA
CCEEEEEECCCEECCCCCHHHHCCHHHHHEEECCEECEEECCCEEECCCCCHHHHHHHHH
AEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
SDPVIKRALVSVSDKTGIVDFCRELSLLGVEVFSTGGTLKTLQDAGIAAASISTITGFP
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCEEEECCCCCHHHHHHCCCCHHHHHHHCCCH
EIMDGRVKTLHPKIHGGLLAVRENPDHVNQANENGISFIDLVVVNLYPFEATVAKPDVTF
HHHCCCCEEECCCCCCCEEEEECCCCHHCCCCCCCCEEEEEEEEEEECCEEEECCCCCCH
EDAIENIDIGGPSMLRSAAKNNESVTVVTDSADYALVLQEMRNNNGATKRETRLALALKV
HHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
FELTSRYDRAIASYLAGAQHEADSSMTVKLERELDMRYGENPHQSAGLYRLTDENGTRSF
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHHHHCCCCCCCCCCEEEEECCCCCCHH
SDYFEKLHGKELSYNNMLDIAAAVSLIEEFRGEEPTVVIIKHTNPCGVAQAPTLAEAYRR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHH
AFSTDTQAPFGGIIAFNHPLDMEAATAVNEIFTEILIAPAFEDGVLEMLMKKKDRRLVRQ
HHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TSALPKGGWEFKSTPFGMLVQERDSKIVTKEDLTVVTKRQPTEEEVADMMFAWKICKHIK
HHCCCCCCCCCCCCCCCCEEECCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHHHHHC
SNTILYVKNRQTFGVGAGQMSRVDSSKIARWKASEVNLDLHGSVVASDAFFPFADGLLAA
CCEEEEEECCCEECCCCCHHHHCCHHHHHEEECCEECEEECCCEEECCCCCHHHHHHHHH
AEAGVTAVIQPGGSIRDNEVIEAADANNLAMVFTGMRHFKH
HHCCCEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA