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The map label for this gene is ispF

Identifier: 119356427

GI number: 119356427

Start: 661886

End: 662362

Strand: Reverse

Name: ispF

Synonym: Cpha266_0591

Alternate gene names: 119356427

Gene position: 662362-661886 (Counterclockwise)

Preceding gene: 119356429

Following gene: 119356425

Centisome position: 21.14

GC content: 49.27

Gene sequence:

>477_bases
ATGCGCACAGGTATAGGAATCGATGTACATCCGTTTGCAGAAGGACGCAAGCTTGTTATTGGCGGAGTAGAAATACCGTC
AGCAAAAGGTCTTGACGGCCACAGCGATGCCGATGTTCTTCTTCATGCCGTCAGTGACGCGCTGCTTGGTGCCGCAGCCC
TTGGAGATATTGGTCTGCATTTTCCCAACACCAGCCAGGAATTTAAGGATATCGACAGCATGATTCTTTTGAAGCATGTA
AAAAAGCTCCTTGACAAAGAGGGGTTCCGGATTGTAAATATCGATGCCATGCTGCTTCTTGAAGCGCCGAAAATCGCGTC
GTACATCAACGAGATGAGAAAGAATATTGCCCGCTGTCTCGGCCTTGAACTCAATGCCGTATCGGTGAAAGCAACAACAA
ACGAAAAGCTCGGCTACATCGGCAGAGAAGAAGGCGCCGCAGCTCATGCCGTCTGCCTTATTCAGGTAAAGCCCTAA

Upstream 100 bases:

>100_bases
AAAAATCGAGAACCGAGTCCATCCTTCCTGTTCTGTTTTTTCATGATTTATTCTATCTTAAAGCTCATTTTCTTGAAACA
CTTTAATCATCAGGAACTCC

Downstream 100 bases:

>100_bases
GCGCCGACCTCGCCGCGCAAAATACTTACCGGAAGATAACTGGTCGCCTTTCTTGCCGGGCTGAATGATGAAATTACGGC
AATAAGAACACCAAACACGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MRTGIGIDVHPFAEGRKLVIGGVEIPSAKGLDGHSDADVLLHAVSDALLGAAALGDIGLHFPNTSQEFKDIDSMILLKHV
KKLLDKEGFRIVNIDAMLLLEAPKIASYINEMRKNIARCLGLELNAVSVKATTNEKLGYIGREEGAAAHAVCLIQVKP

Sequences:

>Translated_158_residues
MRTGIGIDVHPFAEGRKLVIGGVEIPSAKGLDGHSDADVLLHAVSDALLGAAALGDIGLHFPNTSQEFKDIDSMILLKHV
KKLLDKEGFRIVNIDAMLLLEAPKIASYINEMRKNIARCLGLELNAVSVKATTNEKLGYIGREEGAAAHAVCLIQVKP
>Mature_158_residues
MRTGIGIDVHPFAEGRKLVIGGVEIPSAKGLDGHSDADVLLHAVSDALLGAAALGDIGLHFPNTSQEFKDIDSMILLKHV
KKLLDKEGFRIVNIDAMLLLEAPKIASYINEMRKNIARCLGLELNAVSVKATTNEKLGYIGREEGAAAHAVCLIQVKP

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=50.6493506493506, Blast_Score=139, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_CHLPD (A1BE20)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_911071.1
- ProteinModelPortal:   A1BE20
- SMR:   A1BE20
- STRING:   A1BE20
- GeneID:   4568711
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0591
- NMPDR:   fig|290317.7.peg.620
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16918; Mature: 16918

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTGIGIDVHPFAEGRKLVIGGVEIPSAKGLDGHSDADVLLHAVSDALLGAAALGDIGLH
CCCCCCCEECCCCCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
FPNTSQEFKDIDSMILLKHVKKLLDKEGFRIVNIDAMLLLEAPKIASYINEMRKNIARCL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEECHHHHHHHHHHHHHHHHHH
GLELNAVSVKATTNEKLGYIGREEGAAAHAVCLIQVKP
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MRTGIGIDVHPFAEGRKLVIGGVEIPSAKGLDGHSDADVLLHAVSDALLGAAALGDIGLH
CCCCCCCEECCCCCCCEEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
FPNTSQEFKDIDSMILLKHVKKLLDKEGFRIVNIDAMLLLEAPKIASYINEMRKNIARCL
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECEEEEEECHHHHHHHHHHHHHHHHHH
GLELNAVSVKATTNEKLGYIGREEGAAAHAVCLIQVKP
CCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA