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The map label for this gene is pfp [H]

Identifier: 119356425

GI number: 119356425

Start: 659232

End: 660437

Strand: Reverse

Name: pfp [H]

Synonym: Cpha266_0589

Alternate gene names: 119356425

Gene position: 660437-659232 (Counterclockwise)

Preceding gene: 119356427

Following gene: 119356424

Centisome position: 21.07

GC content: 48.84

Gene sequence:

>1206_bases
GTGAAAAAAATCGGAATCCTGACCAGCGGCGGGGATTGCGGAGGACTCAATGCCGTGCTGAAAGGCGCAGCACTGATGGC
GCAGTCAAAAAATCTCGAAATGTACATTATTCCAAACGGGTATGCGGGATTGTACAACCTTATCGATCAGAACAGACTTG
TCAGACTTGACAGTGAACGTCTTGACAGATTTGACGCAAGTTTTGCCGGTTCCGAAGCTGGCCATTCAAGAGTTAAAATC
AAGGCTATCAGCAATCCGGAAAAATATGCCCGCATCAAGGCCGGTATGAAAAAATTCGAGCTTGACGCCCTCATCATCAG
CGGCGGAGATGATTCCGGAAGCGTTATGATTGATCTCAACCATAACGGTATTCAGTGTATTCATGCACCAAAAACGATGG
ACCTTGACCTGCAGACCTATTCGGTTGGCGGTGATTCAACCATCAATCGTATAGCCCAGTTTGTTCACGATCTCAAAACC
ACGGGAAAAACCCATAACAGGGTGCTGGTAACCGAAGTATTCGGACGATATGCCGGTCACACGGCATTCCGCAGTGGTAT
TGCCGCAGAAGCCGACTGTATTCTTATACCCGAGATTCCTGTTGACTGGAATGCTGTCTATGAGCATCTTGCAGAACGCT
TTACCCGCCGCATCAGAGAAAGCGACGTCCATAGCGGCACCTACACGATCGTTGTAGCAGAAGGAATGAAAAACGCAGAT
GGCACCGATATTGTTGATGAATCCGCAGGTCTCGATGCATTTGGACACAAAAAACTTGCCGGAGCCGGAAAATATACCTG
CCAGCAAATTCAGAAAAACATGAAGGCCGACCCTGCCATGCCGATCTTCATGAAAGAGACCGGCATGTTTGTGGAAGGCA
TTTATGAAATACCGGAAGTCCGTGAAATCCATCCCGGTCACCTTGTCCGTTCCGGAAACTCGTCAGCCTACGATATCAAC
TTCGGCTTTGAAGCAGGGGCTGCGGCAGTGCTTCTCCTTCTTGACGGCAAAACAGGCGTGACGATATCAAAAGTCAAGGG
TCGCAAAGTAGAATATATCGAATCAAGCAAAGCTATCATGCAGCGCTATGTCGACCTTGAGCATGTCGCCATGTACGAAG
CTCTCGGAATATGTTTCGGACGCAAACCCGTTGCCTACGATCCAATCCTCCGCGAAGTTGACGGCGTTTACGAACGCATC
TACTGA

Upstream 100 bases:

>100_bases
AAAATCTATCTTGAGTGCCGGAGAGGAAAACAGCTCTTCATGAAAATTTAAATAATTTCGGCCGTAAAAAAACTAACCTT
AGCAATCAAGGAGTGGAACA

Downstream 100 bases:

>100_bases
TTCTCCCCCCATCCTTTTAACCGGCCAGGCCCTGCCGCATCCCCGCGAAGGCCTGGCGCTGGTTACGCCTATGGCATCGA
TGCACTCAATCATAGACCAA

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: 6-phosphofructokinase, pyrophosphate dependent; PPi-dependent phosphofructokinase; PPi-PFK; Pyrophosphate-dependent 6-phosphofructose-1-kinase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKKIGILTSGGDCGGLNAVLKGAALMAQSKNLEMYIIPNGYAGLYNLIDQNRLVRLDSERLDRFDASFAGSEAGHSRVKI
KAISNPEKYARIKAGMKKFELDALIISGGDDSGSVMIDLNHNGIQCIHAPKTMDLDLQTYSVGGDSTINRIAQFVHDLKT
TGKTHNRVLVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWNAVYEHLAERFTRRIRESDVHSGTYTIVVAEGMKNAD
GTDIVDESAGLDAFGHKKLAGAGKYTCQQIQKNMKADPAMPIFMKETGMFVEGIYEIPEVREIHPGHLVRSGNSSAYDIN
FGFEAGAAAVLLLLDGKTGVTISKVKGRKVEYIESSKAIMQRYVDLEHVAMYEALGICFGRKPVAYDPILREVDGVYERI
Y

Sequences:

>Translated_401_residues
MKKIGILTSGGDCGGLNAVLKGAALMAQSKNLEMYIIPNGYAGLYNLIDQNRLVRLDSERLDRFDASFAGSEAGHSRVKI
KAISNPEKYARIKAGMKKFELDALIISGGDDSGSVMIDLNHNGIQCIHAPKTMDLDLQTYSVGGDSTINRIAQFVHDLKT
TGKTHNRVLVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWNAVYEHLAERFTRRIRESDVHSGTYTIVVAEGMKNAD
GTDIVDESAGLDAFGHKKLAGAGKYTCQQIQKNMKADPAMPIFMKETGMFVEGIYEIPEVREIHPGHLVRSGNSSAYDIN
FGFEAGAAAVLLLLDGKTGVTISKVKGRKVEYIESSKAIMQRYVDLEHVAMYEALGICFGRKPVAYDPILREVDGVYERI
Y
>Mature_401_residues
MKKIGILTSGGDCGGLNAVLKGAALMAQSKNLEMYIIPNGYAGLYNLIDQNRLVRLDSERLDRFDASFAGSEAGHSRVKI
KAISNPEKYARIKAGMKKFELDALIISGGDDSGSVMIDLNHNGIQCIHAPKTMDLDLQTYSVGGDSTINRIAQFVHDLKT
TGKTHNRVLVTEVFGRYAGHTAFRSGIAAEADCILIPEIPVDWNAVYEHLAERFTRRIRESDVHSGTYTIVVAEGMKNAD
GTDIVDESAGLDAFGHKKLAGAGKYTCQQIQKNMKADPAMPIFMKETGMFVEGIYEIPEVREIHPGHLVRSGNSSAYDIN
FGFEAGAAAVLLLLDGKTGVTISKVKGRKVEYIESSKAIMQRYVDLEHVAMYEALGICFGRKPVAYDPILREVDGVYERI
Y

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI266453768, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI266453748, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI4505749, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI266453619, Length=365, Percent_Identity=26.027397260274, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI48762920, Length=366, Percent_Identity=24.0437158469945, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI11321601, Length=272, Percent_Identity=25.3676470588235, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790350, Length=349, Percent_Identity=28.6532951289398, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012829
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.90 [H]

Molecular weight: Translated: 43948; Mature: 43948

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIGILTSGGDCGGLNAVLKGAALMAQSKNLEMYIIPNGYAGLYNLIDQNRLVRLDSER
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHCCCCEEEECHHH
LDRFDASFAGSEAGHSRVKIKAISNPEKYARIKAGMKKFELDALIISGGDDSGSVMIDLN
HHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCHHHEEEEEEEEECCCCCCEEEEEEC
HNGIQCIHAPKTMDLDLQTYSVGGDSTINRIAQFVHDLKTTGKTHNRVLVTEVFGRYAGH
CCCEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGIAAEADCILIPEIPVDWNAVYEHLAERFTRRIRESDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GTDIVDESAGLDAFGHKKLAGAGKYTCQQIQKNMKADPAMPIFMKETGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCC
REIHPGHLVRSGNSSAYDINFGFEAGAAAVLLLLDGKTGVTISKVKGRKVEYIESSKAIM
CCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEHHHHHHHHH
QRYVDLEHVAMYEALGICFGRKPVAYDPILREVDGVYERIY
HHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIGILTSGGDCGGLNAVLKGAALMAQSKNLEMYIIPNGYAGLYNLIDQNRLVRLDSER
CCCEEEEECCCCCCCHHHHHHHHHHEEECCCCEEEEEECCCHHHHHHHCCCCEEEECHHH
LDRFDASFAGSEAGHSRVKIKAISNPEKYARIKAGMKKFELDALIISGGDDSGSVMIDLN
HHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCHHHEEEEEEEEECCCCCCEEEEEEC
HNGIQCIHAPKTMDLDLQTYSVGGDSTINRIAQFVHDLKTTGKTHNRVLVTEVFGRYAGH
CCCEEEEECCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCH
TAFRSGIAAEADCILIPEIPVDWNAVYEHLAERFTRRIRESDVHSGTYTIVVAEGMKNAD
HHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
GTDIVDESAGLDAFGHKKLAGAGKYTCQQIQKNMKADPAMPIFMKETGMFVEGIYEIPEV
CCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCC
REIHPGHLVRSGNSSAYDINFGFEAGAAAVLLLLDGKTGVTISKVKGRKVEYIESSKAIM
CCCCCCCEEECCCCCEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEHHHHHHHHH
QRYVDLEHVAMYEALGICFGRKPVAYDPILREVDGVYERIY
HHHHCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10913106 [H]