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The map label for this gene is gltX

Identifier: 119356265

GI number: 119356265

Start: 471873

End: 473381

Strand: Direct

Name: gltX

Synonym: Cpha266_0427

Alternate gene names: 119356265

Gene position: 471873-473381 (Clockwise)

Preceding gene: 119356261

Following gene: 119356266

Centisome position: 15.06

GC content: 48.51

Gene sequence:

>1509_bases
ATGGTTGCTCAAAGGTATAGAACCAGGTTCGCTCCTTCCCCGACAGGGTATCTGCATGTAGGCGGATTGCGGACAGCCCT
GTATAATTACCTGTTTGTTAAAAAGATGAAAGGTGACTTCGTGCTGAGAATTGAAGATACCGATCGGAGCCGCAGAGTTG
AGGGGGCACAGCAAAATTTGCTTAAAACCCTTGAATGGGCGGGAATTGTGCCTGACGAAAGTCCTGAGCTCGGAGGTGAT
TTCGGTCCATATATCCAGTCCGAAAGGCTTGAAATCTACAAGAAATATTGTGACGAGCTCCTTGAAGGGAAATATGCTTA
TTACTGTTTCGCTACATCAGAGGAGCTTGAGGAGAATCGGCAGCTTCAGCTCAAGCAGGGGCTTCAGCCAAAATATAACA
GAAAATGGCTGCCCGAAGAGATGGGGGGATCGATGCCTTCATCGGAAATAAGAAAGAAAATGGAGCAGAACGCCCCCTAT
GTTATTCGCATGAAGGTGCCGGATTATGTTTCCGTCTGGTTTGAAGATCTTATCAGAGGGCCGGTCGAGTTTGATTCATC
GACCATTGACGATCAGGTGCTGATGAAGTCCGACGGGTTTCCGACCTATCACTTTGCCAGTGTTATTGACGATCATCTCA
TGGAGTTTACCCATATCATCAGGGGTGAAGAGTGGCTTCCTTCCATGCCGAAACATCTCCTGCTGTACGAGTTTTTCGGA
TGGGAGCCTCCAAAATTTGCCCATCTTCCCCTGTTGCTCAATCCGGACCGATCAAAACTCAGCAAGCGTCAGGGTGACGT
CGCCGTTGAGGACTATATTCGGAAAGGGTACAGTGCTGAGGCGATCGTGAATTTTGTTGCCATGCTCGGCTGGAACGAAG
GCGAGGGAACCGAGCAGGAAGTATACAGCCTTGAACAGCTTGTTGAGAGGTTTTCGCTTGAACGAGTCGGCAAGGCCGGT
GCCATATTCAATGTCGACAAGCTCAACTGGCTTGAAAAACAGTATATCAAAAGCCGTTCTGCCGATCGTATTATTGCCGT
TATCAAGCCGTTGCTCATGGCGGAACTGGAAAAAAGGCCGACAGCTATGCATGAATCGGTTATTACCAGTGATCTGTATC
TTGAGCAGGTCATAGAGCTGATGCGTGAGCGTGTCGGGTTTGAGCATGAATTCGTGACCTTTTCGCCATACTTTTTCTTT
GACCCAGAGACTTACGAAGAGGAGGGAGTACGCAAAAGATGGACGGCCGAAACCAACACTCTTTTACGTGAGTTTGTAAC
GGTTCTCGCATCTCTTGATGCTTTCACTGCCGAAGCGATTGAAACGGAGCTGAAGGCTTTTGTCGCACCCAAAGGGCTTA
AAGCAGCGGCATTGATCCATCCTCTCAGGATTCTCTGTTCAGGGGTTAGTTTTGGACCAAGTCTCTATCATATGCTTGAG
GTTCTTGGAAAAGATGCCGTTATTCGCCGGATTTCCAGGGGTATTGAAACGGTGGTCTGCCTCTCGTGA

Upstream 100 bases:

>100_bases
GTGCTGTTATTTATGCTGCAAAAACCGGTGAGTTTTTTTAAATTCCCTCCTTCTGACCGTGATCGGCCCTTTATAATAAT
AAACAGTACGGTATCACATT

Downstream 100 bases:

>100_bases
ATAAATGATGATTGGCGGAGTTGCGGTTGAAGCGTTTTTGTTTTGACTTCATTGTAAAATTGCTTATTATAAGCGCAACT
CTTAAGGACAGATAGCTCAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MVAQRYRTRFAPSPTGYLHVGGLRTALYNYLFVKKMKGDFVLRIEDTDRSRRVEGAQQNLLKTLEWAGIVPDESPELGGD
FGPYIQSERLEIYKKYCDELLEGKYAYYCFATSEELEENRQLQLKQGLQPKYNRKWLPEEMGGSMPSSEIRKKMEQNAPY
VIRMKVPDYVSVWFEDLIRGPVEFDSSTIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYIRKGYSAEAIVNFVAMLGWNEGEGTEQEVYSLEQLVERFSLERVGKAG
AIFNVDKLNWLEKQYIKSRSADRIIAVIKPLLMAELEKRPTAMHESVITSDLYLEQVIELMRERVGFEHEFVTFSPYFFF
DPETYEEEGVRKRWTAETNTLLREFVTVLASLDAFTAEAIETELKAFVAPKGLKAAALIHPLRILCSGVSFGPSLYHMLE
VLGKDAVIRRISRGIETVVCLS

Sequences:

>Translated_502_residues
MVAQRYRTRFAPSPTGYLHVGGLRTALYNYLFVKKMKGDFVLRIEDTDRSRRVEGAQQNLLKTLEWAGIVPDESPELGGD
FGPYIQSERLEIYKKYCDELLEGKYAYYCFATSEELEENRQLQLKQGLQPKYNRKWLPEEMGGSMPSSEIRKKMEQNAPY
VIRMKVPDYVSVWFEDLIRGPVEFDSSTIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYIRKGYSAEAIVNFVAMLGWNEGEGTEQEVYSLEQLVERFSLERVGKAG
AIFNVDKLNWLEKQYIKSRSADRIIAVIKPLLMAELEKRPTAMHESVITSDLYLEQVIELMRERVGFEHEFVTFSPYFFF
DPETYEEEGVRKRWTAETNTLLREFVTVLASLDAFTAEAIETELKAFVAPKGLKAAALIHPLRILCSGVSFGPSLYHMLE
VLGKDAVIRRISRGIETVVCLS
>Mature_502_residues
MVAQRYRTRFAPSPTGYLHVGGLRTALYNYLFVKKMKGDFVLRIEDTDRSRRVEGAQQNLLKTLEWAGIVPDESPELGGD
FGPYIQSERLEIYKKYCDELLEGKYAYYCFATSEELEENRQLQLKQGLQPKYNRKWLPEEMGGSMPSSEIRKKMEQNAPY
VIRMKVPDYVSVWFEDLIRGPVEFDSSTIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYIRKGYSAEAIVNFVAMLGWNEGEGTEQEVYSLEQLVERFSLERVGKAG
AIFNVDKLNWLEKQYIKSRSADRIIAVIKPLLMAELEKRPTAMHESVITSDLYLEQVIELMRERVGFEHEFVTFSPYFFF
DPETYEEEGVRKRWTAETNTLLREFVTVLASLDAFTAEAIETELKAFVAPKGLKAAALIHPLRILCSGVSFGPSLYHMLE
VLGKDAVIRRISRGIETVVCLS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=36.5461847389558, Blast_Score=310, Evalue=1e-84,
Organism=Escherichia coli, GI1788743, Length=498, Percent_Identity=32.5301204819277, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI226510925, Length=311, Percent_Identity=30.8681672025724, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1786895, Length=361, Percent_Identity=22.7146814404432, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=498, Percent_Identity=37.14859437751, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6324540, Length=513, Percent_Identity=35.2826510721248, Blast_Score=304, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321192, Length=321, Percent_Identity=25.5451713395639, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=38.1526104417671, Blast_Score=340, Evalue=2e-93,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_CHLPD (A1BDK8)

Other databases:

- EMBL:   CP000492
- RefSeq:   YP_910909.1
- ProteinModelPortal:   A1BDK8
- SMR:   A1BDK8
- STRING:   A1BDK8
- GeneID:   4570981
- GenomeReviews:   CP000492_GR
- KEGG:   cph:Cpha266_0427
- NMPDR:   fig|290317.7.peg.438
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57877; Mature: 57877

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 262-262

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAQRYRTRFAPSPTGYLHVGGLRTALYNYLFVKKMKGDFVLRIEDTDRSRRVEGAQQNL
CCCHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LKTLEWAGIVPDESPELGGDFGPYIQSERLEIYKKYCDELLEGKYAYYCFATSEELEENR
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHH
QLQLKQGLQPKYNRKWLPEEMGGSMPSSEIRKKMEQNAPYVIRMKVPDYVSVWFEDLIRG
HHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PVEFDSSTIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
CCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHC
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYIRKGYSAEAIVNFVAMLGWNEGEGTEQE
CCCCCCCCCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
VYSLEQLVERFSLERVGKAGAIFNVDKLNWLEKQYIKSRSADRIIAVIKPLLMAELEKRP
HHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TAMHESVITSDLYLEQVIELMRERVGFEHEFVTFSPYFFFDPETYEEEGVRKRWTAETNT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCHHHHCHHHHHCHHHHH
LLREFVTVLASLDAFTAEAIETELKAFVAPKGLKAAALIHPLRILCSGVSFGPSLYHMLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGKDAVIRRISRGIETVVCLS
HHCHHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MVAQRYRTRFAPSPTGYLHVGGLRTALYNYLFVKKMKGDFVLRIEDTDRSRRVEGAQQNL
CCCHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
LKTLEWAGIVPDESPELGGDFGPYIQSERLEIYKKYCDELLEGKYAYYCFATSEELEENR
HHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECHHHHHHHH
QLQLKQGLQPKYNRKWLPEEMGGSMPSSEIRKKMEQNAPYVIRMKVPDYVSVWFEDLIRG
HHHHHHCCCCCCCCCCCCHHHCCCCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCC
PVEFDSSTIDDQVLMKSDGFPTYHFASVIDDHLMEFTHIIRGEEWLPSMPKHLLLYEFFG
CCCCCCCCCCCHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHHHC
WEPPKFAHLPLLLNPDRSKLSKRQGDVAVEDYIRKGYSAEAIVNFVAMLGWNEGEGTEQE
CCCCCCCCCCEEECCCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHH
VYSLEQLVERFSLERVGKAGAIFNVDKLNWLEKQYIKSRSADRIIAVIKPLLMAELEKRP
HHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
TAMHESVITSDLYLEQVIELMRERVGFEHEFVTFSPYFFFDPETYEEEGVRKRWTAETNT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEECCCCHHHHCHHHHHCHHHHH
LLREFVTVLASLDAFTAEAIETELKAFVAPKGLKAAALIHPLRILCSGVSFGPSLYHMLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
VLGKDAVIRRISRGIETVVCLS
HHCHHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA